Abstract：
:Lepidopteran nucleopolyhedroviruses have distinct viral tissue tropisms in host larvae. We previously identified the Bm8 gene of Bombyx mori nucleopolyhedrovirus (BmNPV), the product of which inhibits viral propagation in the middle silk gland (MSG). However, it is unknown whether this inhibitory function of the Bm8 protein is specific to MSGs. Here we generated a Bm8-disrupted recombinant BmNPV expressing green fluorescent protein (GFP) and examined viral propagation in B. mori cultured cells and larvae. We found that Bm8-disrupted BmNPV produced fewer budded viruses and more occlusion bodies (OBs) than the wild-type virus in both cultured cells and larvae. Microscopic observation of OB production and GFP expression revealed that Bm8 disruption accelerated the progression of viral infection in various larval tissues. Furthermore, quantitative reverse transcription-polymerase chain reaction experiments showed that the loss of Bm8 enhanced viral gene expression in BmNPV-infected larval tissues. These results indicate that the Bm8 protein suppresses viral propagation to varying degrees in each larval tissue, which may establish BmNPV tissue tropisms in B. mori larvae.
journal_name
Virus Resjournal_title
Virus researchauthors
Hikida H,Kokusho R,Kobayashi J,Shimada T,Katsuma Sdoi
10.1016/j.virusres.2018.03.011subject
Has Abstractpub_date
2018-04-02 00:00:00pages
124-131eissn
0168-1702issn
1872-7492pii
S0168-1702(17)30774-8journal_volume
249pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract：:The uptake of porcine parvovirus (PPV) into host cells, permissive or nonpermissive for PPV replication, was monitored by autoradiography, immunofluorescent microscopy, and dual parameter flow cytometry. While both permissive and nonpermissive cells selectively took up the light (activated) form of the virus from a mi...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(88)90054-8
更新日期：1988-04-01 00:00:00
abstract：:By Western blot analyzes of expression of avian reovirus proteins, one unknown fragment was detected by an anti-sigmaA monoclonal antibody in virus-infected cells lysate. It was interesting to note that RNA interference against sigmaA resulted in the suppression of the unknown fragment. Using various lengths of sigmaA...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2010.01.003
更新日期：2010-04-01 00:00:00
abstract：:A simple and sensitive method for the detection of measles virus genome was developed, amplifying the regions encoding the nucleocapsid (N) protein and hemagglutinin (H) protein of measles virus by reverse transcriptase-polymerase chain reaction (RT-PCR). We examined a variety of measles patients: 28 patients with nat...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(94)00074-m
更新日期：1995-01-01 00:00:00
abstract：:Immunisation of goats, mice, rats, rabbits, guinea pigs, and hamsters with the recombinant ectodomain of the porcine endogenous retrovirus (PERV) transmembrane envelope (TM) protein (p15E) induced binding and neutralising immune antibodies in all animals. In contrast, no antibodies were induced when pigs were immunise...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2015.05.019
更新日期：2015-10-02 00:00:00
abstract：:Transplacental viral infections are dependent upon complex interactions between feto-placental and maternal immune responses and the stage of fetal development at which the infection occurs. Bovine viral diarrhea virus (BVDV) has the ability to cross the placenta and infect the fetus. Infection early in gestation with...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.05.018
更新日期：2012-08-01 00:00:00
abstract：:Swine influenza virus (SIV) is not only an important respiratory pathogen in pigs but also a threat to human health. The pandemic influenza A(H1N1)pdm09 virus likely originated in swine through reassortment between a North American triple reassortant and Eurasian avian-like SIV. The North American triple reassortant v...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.01.004
更新日期：2013-05-01 00:00:00
abstract：:Cricket paralysis virus (Dicistroviridae: Cripavirus) (CrPV) naturally has a wide range of insect hosts which is reflected in its ability to infect several cultured insect cell lines. The expression of viral gene products is controlled by two kinds of internal ribosome entry site (IRES) elements, 5' and intergenic (IG...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(03)00139-4
更新日期：2003-08-01 00:00:00
abstract：:Mouse Hepatitis Virus (MHV) is a single-stranded positive sense RNA virus with the ability to promote acute and chronic diseases in mice. The MHV spike protein (S) is a major virulence determinant which in addition to binding to cellular receptors to mediate cell entry and facilitate virus spread to adjacent cells by ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.05.023
更新日期：2014-08-30 00:00:00
abstract：:Four species of Diuris temperate terrestrial orchids from wild and captive populations were tested for the presence of polyadenylated RNA viruses. The genomes of three exotic viruses were determined: two potyviruses, Bean yellow mosaic virus and Ornithogalum mosaic virus, and the polerovirus Turnip yellows virus. The ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.10.003
更新日期：2013-01-01 00:00:00
abstract：:We have studied which steps are enhanced in the infectious cycle of influenza A virus in Madin-Darby canine kidney (MDCK) cells, a cell line investigated for use in the production of an influenza vaccine because of its ability to yield high levels of virus. We have confirmed that MDCK had the highest production levels...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.04.028
更新日期：2008-09-01 00:00:00
abstract：:To examine the regulatory properties of feline immunodeficiency virus (FIV) long terminal repeat (LTR) integrated into host chromatin, Crandell feline kidney cells were stably transfected with the FIV LTR that directs the bacterial chloramphenicol acetyltransferase (CAT) gene. Using these cells, we examined the effect...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(96)01284-1
更新日期：1996-04-01 00:00:00
abstract：:Cytoplasmic RNA granules consist of microscopic agglomerates of mRNAs and proteins and occur when the translation is reversibly and temporally halted (stress granules, SGs) or mRNAs are targeted for decapping (processing bodies, PBs). The induction of RNA granules formation by virus infection is a common feature of ma...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2019.197823
更新日期：2020-01-15 00:00:00
abstract：:A temperature-sensitive mutant of the Moloney murine leukemia virus-TB, ts1, causes hindlimb paralysis and immunodeficiency in mice. At the restrictive temperature, the envelope precursor polyprotein, gPr80env, is inefficiently processed intracellularly, and this is associated with the neurovirulence of ts1. To test t...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(91)90096-e
更新日期：1991-03-01 00:00:00
abstract：:The nucleotide sequence of the matrix gene (M) of a recently identified morbillivirus, phocid distemper virus (PDV), was determined and the amino acid composition deduced. The M gene of PDV shared many characteristics with the corresponding gene in other morbilliviruses. The nucleotide homology with the closely relate...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(92)90064-g
更新日期：1992-04-01 00:00:00
abstract：:Equine rhinitis A virus (ERAV) is a picornavirus which causes an acute respiratory infection in horses worldwide, and virus neutralization (VN) has been the standard method for the detection of ERAV antibody in horse serum. Previous studies have identified recombinant virion protein VP1 (rVP1) purified under native co...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.03.003
更新日期：2009-07-01 00:00:00
abstract：:Recent reports indicated presence of two antigenic and genetic groups (genotypes VI and VII) of foot-and-mouth disease virus (FMDV) type A in India and are divergent from the vaccine strains. In order to choose suitable field isolate as candidate vaccine strain, anti-sera against representative isolates from both the ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2005.03.021
更新日期：2005-09-01 00:00:00
abstract：:Bovine enteroviruses, which belong to the Picornaviridae family, can cause clinical symptoms in cattle and are excreted in feces. In this study, a cytolytic virus was isolated from Madin-Darby bovine kidney (MDBK) cells from fecal samples of bovine with severe diarrhea and hemorrhagic intestinal mucosa that had been o...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.07.032
更新日期：2014-10-13 00:00:00
abstract：:Recent evidence has accumulated which definitively shows that chemokine receptors CCR5 and CXCR4 play an essential role as coreceptors for human immunodeficiency virus type 1 (HIV-1) infection. Flow cytometric analysis permitted us to detect CD38, a surface marker of early differentiation, as well as activation of T c...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(00)00220-3
更新日期：2001-01-01 00:00:00
abstract：:The pathogenesis of chorioallantoic membrane (CAM) infection with herpes simplex virus 1 and 2 (HSV-1 and HSV-2) as well as chick embryo fibroblast (CEF) passaged HSV-1 was studied. It was found that HSV-2 is at least a million-fold more virulent than HSV-1 as measured by pfu/LD50 ratios for the embryo. Serial passage...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(86)90017-1
更新日期：1986-08-01 00:00:00
abstract：:Infectious spleen and kidney necrosis virus (ISKNV) is the etiological agent that causes a pandemic and severe disease in fish characteristic of enlarged and damaged spleen and kidney. To identify viral proteins involved in infection and pathogenesis, we characterized five open reading frames (ORFs) of the ISKNV genom...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2006.09.003
更新日期：2007-02-01 00:00:00
abstract：:The E2 glycoprotein of classical swine fever virus (CSFV) is involved in viral infection and induction of neutralizing antibodies. Little is known about how E2 interacts with host cells. To understand the cellular factors involved in viral replication cycle, E2 was used as bait in yeast two-hybrid screens, resulting i...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.10.016
更新日期：2014-01-22 00:00:00
abstract：:The A antigen gene from a very virulent strain of Marek's disease virus, RB-1B, has been cloned and the nucleotide sequence determined. The predicted amino acid sequence showed 99% identity to that determined for the MDV GA A antigen (Coussens and Velicer, J. Virol. 62, 2373-2379, 1988) over all but the carboxy-termin...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90094-4
更新日期：1989-04-01 00:00:00
abstract：:Okra leaf curl disease (OLCD) is the most important viral disease of okra in West Africa. In this study, a complex of begomoviruses and associated DNA satellites were identified in symptomatic okra plants from southwestern Cameroon. Sequence analyses showed that two of the plants (Lik1 and Njo5) were infected with a b...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.03.010
更新日期：2013-06-01 00:00:00
abstract：:The main cellular target for African swine fever virus (ASFV) is the porcine macrophage. However, existing data about the early phases of infection were previously characterized in non-leukocyte cells such as Vero cells. Here, we report that ASFV enters the natural host cell using dynamin-dependent and clathrin-mediat...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2015.01.022
更新日期：2015-03-16 00:00:00
abstract：:Prion diseases are fatal neurodegenerative disorders by which the native cellular prion protein (PrPC) is misfolded into an accumulating, disease-associated isoform (PrPD). To improve the understanding of prion pathogenesis and develop effective treatments, it is essential to elucidate factors contributing to cellular...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2017.07.016
更新日期：2017-08-15 00:00:00
abstract：:Tomato spotted wilt virus (TSWV) is a plant-infecting member of the family Bunyaviridae. TSWV encodes two envelope glycoproteins, G(N) and G(C), which are required for virus infection of the arthropod vector. Other members of the Bunyaviridae enter host cells by pH-dependent endocytosis. During this process, the glyco...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2005.01.007
更新日期：2005-06-01 00:00:00
abstract：:Human parainfluenza virus type 3 (HPIV3) fuses the viral envelope with the host cell membrane through the concerted action of the fusion (F) protein and the hemagglutinin-neuraminidase (HN). Upon HN binding to sialic acid (SA), the F protein in a metastable prefusion form is activated to undergo a series of structural...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2019.197791
更新日期：2020-01-02 00:00:00
abstract：:The complete cDNA sequence corresponding to the genomic RNA of a South American strain of potato virus X (PVXc) is reported. The sequence (6432 nucleotides) contains five open reading frames coding for polypeptides with molecular weights of 165.3, 24.3, 12.3, 7.6 and 25.0 and displays an overall homology of 77.4% with...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(90)90054-f
更新日期：1990-07-01 00:00:00
abstract：:The disulphide-linked GP2/3/4 spike of equine arteritis virus (EAV) is essential for virus entry. We showed recently that in transfected cells carbohydrates attached adjacent to the signal peptide of GP3 inhibit cleavage. Here we confirm this unique phenomenon in recombinant viruses with disabled glycosylation sites. ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.02.005
更新日期：2014-04-01 00:00:00
abstract：:To gain more insights into the epidemiology of hantaviruses in China, the hantaviral S, M and L segment sequences were recovered from two Microtus fortis captured in Shenyang and four M. fortis trapped in Yuanjiang in China. Genetic analysis revealed that Shenyang sequences are closely related to the sequences of Fuso...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.06.012
更新日期：2008-10-01 00:00:00
