当前位置： SCI文献检索 > VIRUS RESEARCH期刊下所有文献 > Signal peptide cleavage from GP3 enabled by removal of adjacent glycosylation sites does not impair replication of equine arteritis virus in cell culture, but the hydrophobic C-terminus is essential.

Signal peptide cleavage from GP3 enabled by removal of adjacent glycosylation sites does not impair replication of equine arteritis virus in cell culture, but the hydrophobic C-terminus is essential.

Abstract：

:The disulphide-linked GP2/3/4 spike of equine arteritis virus (EAV) is essential for virus entry. We showed recently that in transfected cells carbohydrates attached adjacent to the signal peptide of GP3 inhibit cleavage. Here we confirm this unique phenomenon in recombinant viruses with disabled glycosylation sites. Surprisingly, the infectivity of EAV containing GP3 with cleaved signal peptide was not impaired and GP3 with cleaved signal peptide associates with GP2/4 in virus particles. In contrast, viruses containing GP3 with deleted hydrophobic C-terminus rapidly reverted back to wild type. The data support our model that the signal peptide is exposed to the lumen of the ER and the C-terminus peripherally attaches GP3 to membranes.

journal_name

Virus Res

journal_title

Virus research

authors

Matczuk AK,Veit M

doi

10.1016/j.virusres.2014.02.005

subject

Has Abstract

pub_date

2014-04-01 00:00:00

pages

107-11

eissn

0168-1702

issn

1872-7492

pii

S0168-1702(14)00058-6

journal_volume

183

pub_type

杂志文章

相关文献

VIRUS RESEARCH文献大全
  • Phylogenetic and cluster analysis of human rhinovirus species A (HRV-A) isolated from children with acute respiratory infections in Yamagata, Japan.

    abstract：:We performed phylogenetic and cluster analysis of human rhinovirus species A (HRV-A) isolated from 76 children with acute respiratory infection in Yamagata prefecture, Japan during the period 2003-2007. Phylogenetic trees based on the nucleotide and amino acid sequences of the VP4/VP2 coding region showed that the pre...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2009.11.011

    authors： Mizuta K,Hirata A,Suto A,Aoki Y,Ahiko T,Itagaki T,Tsukagoshi H,Morita Y,Obuchi M,Akiyama M,Okabe N,Noda M,Tashiro M,Kimura H

    更新日期：2010-02-01 00:00:00

  • Detection of intersubtype recombinants with respect to env and nef genes of HIV-1 among female sex workers in Calcutta, India.

    abstract：:HIV-1 detected among female sex workers in Calcutta, India was characterized in respect to env and nef genes. A total of 39 HIV-1 seropositive samples were used in the study. Phylogenetic analysis of the nucleotide sequences of respective regions showed that 22 out of 39 samples (56.4%) were infected with subtype C wi...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2007.06.013

    authors： Bhanja P,Sengupta S,Banerjee D,Sarkar K,Jana S,Chakrabarti S

    更新日期：2007-12-01 00:00:00

  • The gene encoding the late nonstructural 36K protein of vaccinia virus is essential for virus reproduction.

    abstract：:Two genetic markers--the thymidine kinase gene of herpes simplex virus, and the beta-galactosidase gene of Escherichia coli--were incorporated into the 36K protein gene (IL1 gene according to the nomenclature of the Copenhagen strain of vaccinia virus; Goebel et al., 1990) from the HindIII-P DNA fragment of the LIVP s...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(93)90027-k

    authors： Shchelkunov SN,Ryazankina OI,Gashnikov PV

    更新日期：1993-06-01 00:00:00

  • Cleavage of the respiratory syncytial virus fusion protein is required for its surface expression: role of furin.

    abstract：:The fusion (F) glycoprotein of respiratory syncytial virus (RSV) is synthesized as a nonfusogenic precursor protein (F(0)), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F(1) and F(2) subunits. In the present study, soluble secreted human furin produced by a recombin...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/s0168-1702(00)00149-0

    authors： Bolt G,Pedersen LO,Birkeslund HH

    更新日期：2000-06-01 00:00:00

  • Early intranuclear replication of African swine fever virus genome modifies the landscape of the host cell nucleus.

    abstract：:Although African swine fever virus (ASFV) replicates in viral cytoplasmic factories, the presence of viral DNA within the host cell nucleus has been previously reported to be essential for productive infection. Herein, we described, for the first time, the intranuclear distribution patterns of viral DNA replication ev...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2015.07.006

    authors： Simões M,Martins C,Ferreira F

    更新日期：2015-12-02 00:00:00

  • RNA structure analysis of alphacoronavirus terminal genome regions.

    abstract：:Coronavirus genome replication is mediated by a multi-subunit protein complex that is comprised of more than a dozen virally encoded and several cellular proteins. Interactions of the viral replicase complex with cis-acting RNA elements located in the 5' and 3'-terminal genome regions ensure the specific replication o...

    journal_title：Virus research

    pub_type： 杂志文章,评审

    doi：10.1016/j.virusres.2014.10.001

    authors： Madhugiri R,Fricke M,Marz M,Ziebuhr J

    更新日期：2014-12-19 00:00:00

  • Molecular phylogeny of all human enterovirus serotypes based on comparison of sequences at the 5' end of the region encoding VP2.

    abstract：:Sixty-six human enterovirus serotypes have been described using antibody neutralization, with antigenic variants defined within several serotypes. Despite the availability of sequence data for numerous enteroviruses, the molecular basis of serotype is unknown. Previous studies by others have identified four major phyl...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/s0168-1702(98)00101-4

    authors： Oberste MS,Maher K,Pallansch MA

    更新日期：1998-11-01 00:00:00

  • MicroRNA-4331-5p promotes FMDV replication through inhibiting interferon pathways in PK-15 cells.

    abstract：:MicroRNAs play vital roles in regulating the battle between pathogens and host cells during viral challenging. MiR-4331 aggravates transmissible gastroenteritis virus (TGEV) -induced mitochondrial damage, also suppresses transcription of TGEV gene 7 via targeting cellular CDCA7. Otherwise, miR-4331-5p affects H1N1/200...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2020.198064

    authors： Ren T,Wang Y,Chen H,Wang K,Gao X,Liu L,Zhang Y,Sun Y

    更新日期：2020-09-01 00:00:00

  • Computational predictions suggest that structural similarity in viral polymerases may lead to comparable allosteric binding sites.

    abstract：:The identification of ligand-binding sites is often the first step in drug targeting and design. To date there are numerous computational tools available to predict ligand binding sites. These tools can guide or mitigate the need for experimental methods to identify binding sites, which often require significant resou...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2016.05.029

    authors： Brown JA,Espiritu MV,Abraham J,Thorpe IF

    更新日期：2016-08-15 00:00:00

  • Four distinct isolates of Helminthosporium victoriae virus 190S identified from Bipolaris maydis.

    abstract：:Helminthosporium victoriae virus 190S (HvV190S) is the type species of the genus Victorivirus under the family Totiviridae. To date, HvV190S has never been found in places outside of the USA and has Helminthosporium victoriae as its only know natural host fungus in the field. Here, we report the identification of 4 do...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2020.197941

    authors： Wu R,Yang Y,Duan X,An H,Du Z,Zhang S,Zhang X

    更新日期：2020-08-01 00:00:00

  • Region coding for subgroup specificity of envelope of avian retroviruses does not determine lymphomagenicity.

    abstract：:The purpose of this study was to test whether the region coding for subgroup envelope specificity was a determinant of oncogenic potential. An avian retrovirus recombinant was constructed to contain the envelope gene (env) determinant for subgroup A of an avian RNA tumor virus in one of the non-oncogenic, endogenous a...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(86)90048-1

    authors： Wright SE,Bennett DD

    更新日期：1986-11-01 00:00:00

  • A sensitive and rapid RNA silencing suppressor activity assay based on alfalfa mosaic virus expression vector.

    abstract：:Plant viruses express RNA silencing suppressor (RSS) proteins to counteract plant defence mechanisms. Here, we describe a method to assess the RSS activity based on an alfalfa mosaic virus (AMV) RNA 3 expression vector and transgenic Nicotiana tabacum plants that express the P1 and P2 subunits of the AMV replicase (P1...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2019.197733

    authors： Martínez-Pérez M,Navarro JA,Pallás V,Sánchez-Navarro JA

    更新日期：2019-10-15 00:00:00

  • Induction of insert-specific immune response in mice by hamster polyomavirus VP1 derived virus-like particles carrying LCMV GP33 CTL epitope.

    abstract：:Hamster polyomavirus (HaPyV) major capsid protein VP1 based chimeric virus-like particles (VLPs) carrying model GP33 CTL epitope derived from Lymphocytic choriomeningitis virus (LCMV) were generated in yeast and examined for their capability to induce CTL response in mice. Chimeric VP1-GP33 VLPs were effectively proce...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2011.08.003

    authors： Mazeike E,Gedvilaite A,Blohm U

    更新日期：2012-01-01 00:00:00

  • Reversion to wildtype of a mutated and nonfunctional coxsackievirus B3CRE(2C).

    abstract：:The cis-acting replication element (CRE) in the 2C protein coding region [CRE(2C)] of enteroviruses (EV) facilitates the addition of two uridine residues (uridylylation) onto the virus-encoded protein VPg in order for it to serve as the RNA replication primer. We demonstrated that coxsackievirus B3 (CVB3) is replicati...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2016.04.016

    authors： Smithee S,Tracy S,Chapman NM

    更新日期：2016-07-15 00:00:00

  • Beta-defensin 2 enhances immunogenicity and protection of an adenovirus-based H5N1 influenza vaccine at an early time.

    abstract：:Reports of human infections with highly pathogenic H5N1 avian influenza viruses in many countries in Asia and Africa with varying case fatality rates highlight the pandemic potential of these viruses. In order to contain a rapidly spreading influenza virus in a pandemic scenario, a vaccine which can induce rapid and r...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2013.09.013

    authors： Vemula SV,Amen O,Katz JM,Donis R,Sambhara S,Mittal SK

    更新日期：2013-12-26 00:00:00

  • Molecular characterization of a novel bat-associated circovirus with a poly-T tract in the 3' intergenic region.

    abstract：:The family Circoviridae comprises a large group of small circular single-stranded DNA viruses with several members causing severe pig and poultry diseases. In recent years the number of new viruses within the family has had an explosive increase showing a high level of genetic diversity and a broad host range. In this...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2018.04.012

    authors： Zhu A,Jiang T,Hu T,Mi S,Zhao Z,Zhang F,Feng J,Fan Q,He B,Tu C

    更新日期：2018-05-02 00:00:00

  • Use of pseudotyped retroviral vectors to analyze the receptor-binding pocket of hemagglutinin from a pathogenic avian influenza A virus (H7 subtype).

    abstract：:The hemagglutinin (HA) protein of influenza virus binds to terminal sialic acid residues present on cell surface glycoproteins and glycolipids. The specific amino acids involved in this interaction have been identified for a H3 subtype HA from the human non-pathogenic virus, A/Aichi/2/68, by both crystallographic and ...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/s0168-1702(01)00407-5

    authors： Lin AH,Cannon PM

    更新日期：2002-02-26 00:00:00

  • Subcellular localization and membrane association of SARS-CoV 3a protein.

    abstract：:SARS-CoV 3a protein was a unique protein of SARS coronavirus (SARS-CoV), which was identified in SARS-CoV infected cells and SARS patients' specimen. Recent studies revealed that 3a could interact specifically with many SARS-CoV structural proteins, such as M, E and S protein. Expressed 3a protein was reported to loca...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2005.01.001

    authors： Yuan X,Li J,Shan Y,Yang Z,Zhao Z,Chen B,Yao Z,Dong B,Wang S,Chen J,Cong Y

    更新日期：2005-05-01 00:00:00

  • Strand-specific compositional asymmetries in double-stranded DNA viruses.

    abstract：:Analysis of 22 complete sequences of double-stranded DNA viruses reveals striking compositional asymmetries between leading and lagging, and between transcribed and non-transcribed strands. In all bi-directionally replicated genomes analyzed, the observed leading strand GC skew (measuring relative excess of guanines v...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/s0168-1702(98)00139-7

    authors： Grigoriev A

    更新日期：1999-03-01 00:00:00

  • The deficiency in nuclear localization signal of Neodiprion lecontei nucleopolyhedrovirus DNA polymerase prevents rescue of viral DNA replication and virus production in dnapol-null Autographa californica multiple nucleopolyhedrovirus.

    abstract：:DNA polymerase (DNApol) is highly conserved in baculovirus and is required for viral DNA replication. However, little is known about gammabaculovirus DNApol. Here DNApol of the gammabaculovirus Neodiprion lecontei nucleopolyhedrovirus (NeleNPV) was cloned into a dnapol-null alphabaculovirus AcMNPV bacmid, creating Bac...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2019.04.005

    authors： Chen G,Fang Y,Yan Q,Li P,Wu L,Feng G

    更新日期：2019-06-01 00:00:00

  • Cervical and anal HPV infections in HIV positive women and men.

    abstract：:The goal of this review is to summarize recently published epidemiological information that contribute to understanding the natural history of cervical and human papillomavirus (HPV) infection and their associated lesions among human immunodeficiency virus (HIV) infected women and men. HIV-positive women and men are m...

    journal_title：Virus research

    pub_type： 杂志文章,评审

    doi：10.1016/s0168-1702(02)00189-2

    authors： de Sanjosé S,Palefsky J

    更新日期：2002-11-01 00:00:00

  • Functionality of the 5'- and intergenic IRES elements of cricket paralysis virus in a range of insect cell lines, and its relationship with viral activities.

    abstract：:Cricket paralysis virus (Dicistroviridae: Cripavirus) (CrPV) naturally has a wide range of insect hosts which is reflected in its ability to infect several cultured insect cell lines. The expression of viral gene products is controlled by two kinds of internal ribosome entry site (IRES) elements, 5' and intergenic (IG...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/s0168-1702(03)00139-4

    authors： Masoumi A,Hanzlik TN,Christian PD

    更新日期：2003-08-01 00:00:00

  • USP7 manipulation by viral proteins.

    abstract：:Ubiquitin Specific Protease 7 (USP7) is a deubiquitinating enzyme (DUB) that plays critical roles in the regulation of many cellular processes including epigenetics, tumour suppression, oncogenesis, DNA damage response, immunity and viral infection. USP7 was the first DUB associated with viral infection. Since then ot...

    journal_title：Virus research

    pub_type： 杂志文章,评审

    doi：10.1016/j.virusres.2020.198076

    authors： Bojagora A,Saridakis V

    更新日期：2020-09-01 00:00:00

  • Expression and regulation of the BKRF2, BKRF3 and BKRF4 genes of Epstein-Barr virus.

    abstract：:The BKRF2, BKRF3 and BKRF4 genes of Epstein-Barr virus (EBV) are located close together in the viral genome, which encode glycoprotein L, uracil-DNA glycosylase and a tegument protein, respectively. Here, we demonstrate that the BKRF2 gene behaves as a true-late lytic gene, whereas the BKRF3 and BKRF4 genes belong to ...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2018.08.005

    authors： Chen LW,Hung CH,Wang SS,Yen JB,Liu AC,Hung YH,Chang PJ

    更新日期：2018-09-02 00:00:00

  • The Ep152R ORF of African swine fever virus strain Georgia encodes for an essential gene that interacts with host protein BAG6.

    abstract：:African swine fever virus (ASFV) is the etiological agent of a contagious and often lethal disease of domestic pigs that has significant economic consequences for the swine industry. The viral genome encodes for more than 150 genes, and only a select few of these genes have been studied in some detail. Here we report ...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2016.07.013

    authors： Borca MV,O'Donnell V,Holinka LG,Rai DK,Sanford B,Alfano M,Carlson J,Azzinaro PA,Alonso C,Gladue DP

    更新日期：2016-09-02 00:00:00

  • Computational prediction and identification of dengue virus-specific CD4(+) T-cell epitopes.

    abstract：:In this study, we tried to identify dengue virus-specific CD4(+) T-cell epitopes, which can induce PBMC (peripheral blood mononuclear cells) isolated from DF convalescent patients (dengue virus type 1 infection) to secrete IFN-gamma. PBMC of DF convalescent patients were stimulated in vitro with dengue virus-derived p...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2007.10.010

    authors： Wen JS,Jiang LF,Zhou JM,Yan HJ,Fang DY

    更新日期：2008-03-01 00:00:00

  • Occurrence, sequence polymorphism and population structure of Circulifer tenellus virus 1 in a field population of the beet leafhopper.

    abstract：:Circulifer tenellus virus 1 (CiTV1) is the prototypical example of an unusual group of dsRNA viruses associated with insects and for which ecological data are lacking. A San Joaquin Valley (SJV), California population of the beet leafhopper (BLH; Circulifer tenellus [Baker]) was sampled for CiTV1 in 2010. Among 365 BL...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2013.06.017

    authors： Spear A,Yokomi R,French R,Stenger DC

    更新日期：2013-09-01 00:00:00

  • Increase of virus yields and releases of Borna disease virus from persistently infected cells.

    abstract：:Borna disease virus grows to low titres in persistently infected cells with an infectious particle to cell ratio of 0.01 to 0.05. Inclusion of n-butyrate in the growth medium enhances infectivity yields up to 1 log. This effect is time and concentration dependent. In hypertonic medium with an excess of NaCl, KCl or Na...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(85)90057-7

    authors： Pauli G,Ludwig H

    更新日期：1985-02-01 00:00:00

  • Conditional gene expression in hepatitis C virus transgenic mice without induction of severe liver injury using a non-inflammatory Cre-expressing adenovirus.

    abstract：:We previously established inducible-hepatitis C virus (HCV) transgenic mice, which expressed the HCV gene (nucleotides 294-3435) encoding the core, E1, E2, and NS2 proteins. The expression of these proteins is regulated by the Cre/loxP system and an adenovirus vector (AdV) that expresses Cre DNA recombinase (Cre) cont...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2011.05.019

    authors： Chiyo T,Sekiguchi S,Hayashi M,Tobita Y,Kanegae Y,Saito I,Kohara M

    更新日期：2011-09-01 00:00:00

  • Interferon-mediated inhibition of retroviral infection: use of a defective retrovirus carrying a drug-resistance gene.

    abstract：:We here report the results of an investigation of the effect of interferon on the establishment of new infections by a retrovirus. For this study, we used an infectious but replication-incompetent retrovirus carrying a drug-resistance gene and assayed for infectivity by measuring drug-resistant colony formation. Mouse...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(89)90075-0

    authors： Kumar R,Sen GC

    更新日期：1989-08-01 00:00:00