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Computational prediction and identification of dengue virus-specific CD4(+) T-cell epitopes.

Abstract：

:In this study, we tried to identify dengue virus-specific CD4(+) T-cell epitopes, which can induce PBMC (peripheral blood mononuclear cells) isolated from DF convalescent patients (dengue virus type 1 infection) to secrete IFN-gamma. PBMC of DF convalescent patients were stimulated in vitro with dengue virus-derived peptides, which were prepared based on the prediction of dengue virus-specific CD4(+) T-cell epitopes by using RANKpep online software. Subsequently, the frequency of IFN-gamma producing T cells and percentage of IFN-gamma(+) CD4(+) T cells were measured by using ELISPOT assay and ICS assay (intracellular cytokine straining), respectively. The positive response of PBMC by ELISPOT showed that the numbers of SFC (spots forming cells) ranged from 50 to 310 SFC/1x10(6) PBMC. The positive response of PBMC by ICS assay showed that the percentage of IFN-gamma(+) CD4(+) T cells ranged from 0.03 to 0.27%. As a result, C(45-57) (KLVMAFIAFLRFL), E(396-408) (SSIGKMFEATARG), NS3(23-35) (YRILQRGLLGRSQ), and NS3(141-155) (NREGKIVGLYGNGVV) were identified as dengue virus-specific CD4(+) T-cell epitopes.

journal_name

Virus Res

journal_title

Virus research

authors

Wen JS,Jiang LF,Zhou JM,Yan HJ,Fang DY

doi

10.1016/j.virusres.2007.10.010

subject

Has Abstract

pub_date

2008-03-01 00:00:00

pages

42-8

issue

1-2

eissn

0168-1702

issn

1872-7492

pii

S0168-1702(07)00373-5

journal_volume

132

pub_type

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