Abstract:
:The knowledge of parainfluenza type 5 (PIV-5) virion morphology is essentially based on the observation of negatively stained preparations in conventional transmission electron microscopy (CTEM). In this study, the ultrastructure of frozen-hydrated intact PIV-5 was examined by cryo-electron microscopy (cryo-EM). Cryo-EM revealed a majority of spherical virions (70%), with a lower pleiomorphy than originally observed in CTEM. Phospholipid bilayer thickness, spike length and glycoprotein spikes density were measured. About 2000 glycoprotein spikes were present in an average-sized spherical virion. Altogether, these data depict a more precise view of PIV-5 morphology.
journal_name
Virus Resjournal_title
Virus researchauthors
Terrier O,Rolland JP,Rosa-Calatrava M,Lina B,Thomas D,Moules Vdoi
10.1016/j.virusres.2008.12.017subject
Has Abstractpub_date
2009-06-01 00:00:00pages
200-3issue
1-2eissn
0168-1702issn
1872-7492pii
S0168-1702(09)00011-2journal_volume
142pub_type
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