Abstract：
:The adenovirus type 2 protease (EP) was expressed by infecting insect cells with a recombinant baculovirus. Immunoblot and activity analysis showed EP to be present in both the nucleus and cytoplasm. While the insect cell expressed EP was more soluble than the Escherichia coli expressed EP, its activity was one quarter of the latter, suggesting that eukaryotic postsynthetic modifications are not essential for enzyme activity. EP inactivated a cytoplasmic cathepsin-like baculovirus-encoded cysteine protease which carries a single EP cleavage site and which was capable of digesting most adenovirus structural proteins in vitro. In addition to cleavage of the baculovirus protease, the adenovirus EP was also able to cleave ovalbumin and canine adenovirus protein pre-VII, in the absence of activating peptide. EP activation therefore may occur by means of factors other than the specific activating peptide.
journal_name
Virus Resjournal_title
Virus researchauthors
Keyvani-Amineh H,Labrecque P,Cai F,Carstens EB,Weber JMdoi
10.1016/0168-1702(95)00018-lsubject
Has Abstractpub_date
1995-07-01 00:00:00pages
87-97issue
2eissn
0168-1702issn
1872-7492pii
0168-1702(95)00018-Ljournal_volume
37pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract：:Nearly 100 years after its discovery poliovirus remains one of most thoroughly studied and best understood virus models for the molecular virologist. While poliovirus has been of vital importance for our insight into picornavirus biology at the cellular and biochemical level, it is ironic to note that, due to the earl...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2005.04.008
更新日期：2005-08-01 00:00:00
abstract：:A disease of Rudbeckia hirta (Black-eyed Susan), characterized by severe flower deformation, was observed in Minnesota during 2010-2016. A previously undescribed virus species, named Rudbeckia flower distortion virus (RuFDV, family Caulimoviridae, genus unassigned), was determined to be the causal agent of the disease...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2017.05.012
更新日期：2017-09-15 00:00:00
abstract：:A recent study has shown that the reovirus lambda1 protein can unwind double-stranded nucleic acid molecules, a process energetically coupled to the hydrolysis of nucleoside 5'-triphosphates. In the present study, it was demonstrated that lambda1, expressed as a fusion protein with the Escherichia coli maltose-binding...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(97)00092-0
更新日期：1997-10-01 00:00:00
abstract：:I have observed a relationship between GC content in coding sequences of RNA viruses and their genome polarity. Positive-stranded RNA viruses have significantly higher GC contents than negative-stranded RNA viruses. Coding sequences of all negative-stranded RNA viruses are biased toward high A in coding strands (high ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.10.004
更新日期：2005-04-01 00:00:00
abstract：:To gain more insights into the epidemiology of hantaviruses in China, the hantaviral S, M and L segment sequences were recovered from two Microtus fortis captured in Shenyang and four M. fortis trapped in Yuanjiang in China. Genetic analysis revealed that Shenyang sequences are closely related to the sequences of Fuso...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.06.012
更新日期：2008-10-01 00:00:00
abstract：:To characterize the genomic mutations of hepatitis E virus (HEV) during consecutive passages associated with adaptation to growth in cell culture, a cloned genotype 3 HEV [pJE03-1760F/wt, starting virus (SV)] was passaged 10 times in A549 cells, and the entire genomic sequence of the passage 10 (P10) progeny was deter...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.07.011
更新日期：2016-09-02 00:00:00
abstract：:West Nile virus (WNV) and Japanese encephalitis virus (JEV), the members of JEV serocomplex group are pathogens of global health concern. The co-circulation of these viruses poses challenges in effective diagnostics due to antigenic similarity between the E-protein of these viruses. The present study aimed to design c...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2011.08.008
更新日期：2012-01-01 00:00:00
abstract：:A panel of 6 neutralizing monoclonal antibodies (MAbs) raised against A/Moscow/IIV01/2009 (H1N1) virus isolated during the 2009 pandemic was used for the selection of 26 escape mutants. The mutants were characterized in immune cross-reactions with the panel of MAbs. The sequencing of the mutant HA genes revealed 5 ami...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.03.003
更新日期：2012-06-01 00:00:00
abstract：:A fragment of DNA from within the minimum transforming region (mtr-1) of herpes simplex virus type 1 (HSV-1) is known to raise the mutation frequency of cells. This activity has been attributed to a viral protein whose properties are largely unknown. Antiserum was raised to a synthetic peptide of a predicted amino aci...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(94)90093-0
更新日期：1994-11-01 00:00:00
abstract：:In mice Theiler's murine encephalomyelitis virus (TMEV) persists in macrophages that eventually undergo apoptosis. TMEV infection of macrophages in culture induces apoptosis through the intrinsic pathway, restricting virus yields. We show that inhibition of TMEV-induced apoptosis leads to phosphorylation of receptor i...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.10.017
更新日期：2015-01-02 00:00:00
abstract：:In this study, we tried to identify dengue virus-specific CD4(+) T-cell epitopes, which can induce PBMC (peripheral blood mononuclear cells) isolated from DF convalescent patients (dengue virus type 1 infection) to secrete IFN-gamma. PBMC of DF convalescent patients were stimulated in vitro with dengue virus-derived p...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2007.10.010
更新日期：2008-03-01 00:00:00
abstract：:Two lines of LLC-MK2 cells persistently infected with human parainfluenza virus 3 (HPIV-3) have been maintained in culture for approximately 3 years. Subgenomic RNAs (putative defective interfering particle genomes) were detected in virions released from both persistently infected cultures. In one of the persistently ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(90)90039-e
更新日期：1990-04-01 00:00:00
abstract：:Herpes simplex virus type 1 (HSV-1) establishes a lifelong latency in the neurons of its host. Sporadically, the latent virus reactivates and spreads back to the original site of infection and causes recrudescent diseases. The only gene actively transcribed during neuronal latency is the latency associated transcript ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.08.011
更新日期：2005-03-01 00:00:00
abstract：:A study of the evolutionary relationships of GLRaV-4,-5,-6 and -9, and two new Ampelovirus isolates (GLRaV-Pr and -De) related to grapevine leafroll disease was conducted based on molecular variability, positive selection analysis and maximum likelihood phylogenetic reconstructions. Sequences corresponding to the N-te...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.02.015
更新日期：2008-07-01 00:00:00
abstract：:The disulphide-linked GP2/3/4 spike of equine arteritis virus (EAV) is essential for virus entry. We showed recently that in transfected cells carbohydrates attached adjacent to the signal peptide of GP3 inhibit cleavage. Here we confirm this unique phenomenon in recombinant viruses with disabled glycosylation sites. ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.02.005
更新日期：2014-04-01 00:00:00
abstract：:Zika virus is an emergent arbovirus that has caused a public health emergency in South America. Zika virus infection is known to cause microcephaly and other congenital defects and Guillain-Barré syndrome. Unfortunately no direct antiviral treatments are available at present. IFN-stimulated gene 15 (ISG15) is one of t...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2020.198087
更新日期：2020-10-02 00:00:00
abstract：:The fidelity of poliovirus RNA-dependent RNA polymerase (3Dpol) was determined using a modified alpha-complementation assay. Several polymerases have been analyzed by this approach allowing comparisons to be drawn. Various conditions including high and low MgCl(2), replacing MgCl(2) with MnCl(2), skewed nucleotide poo...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.10.006
更新日期：2010-01-01 00:00:00
abstract：:Immunisation of goats, mice, rats, rabbits, guinea pigs, and hamsters with the recombinant ectodomain of the porcine endogenous retrovirus (PERV) transmembrane envelope (TM) protein (p15E) induced binding and neutralising immune antibodies in all animals. In contrast, no antibodies were induced when pigs were immunise...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2015.05.019
更新日期：2015-10-02 00:00:00
abstract：:The complete nucleotide sequence has been determined for the S RNA of Aino virus, a member of the Simbu serogroup (Bunyavirus genus, family Bunyaviridae). The S RNA is 850 nucleotides long (2.76 X 10(5) daltons) and in the viral complementary sequence has a short 5' non-coding region of 34 nucleotides and a more exten...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(84)90034-0
更新日期：1984-01-01 00:00:00
abstract：:Tick-borne encephalitis (TBE) is a serious human neurological disease caused by TBE virus (TBEV). However, the mechanisms of TBEV-caused pathogenesis remain unclear. The endoplasmic reticulum (ER) stress response, also defined as the unfolded protein response (UPR), is an important conserved molecular signaling pathwa...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.10.012
更新日期：2013-12-26 00:00:00
abstract：:Hepatitis delta virus (HDV) is an RNA virus and eight clades of HDV have been identified. HDV clade 3 (HDV-3) is isolated only in the northern area of South America. The outcome of HDV-3 infection is associated with severe fulminant hepatitis. Variations in the large delta antigen (LDAg) between HDV clade 1 (HDV-1) an...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.08.021
更新日期：2012-12-01 00:00:00
abstract：:When the hepatitis B virus (HBV) enters target cells, there are complex trans-regulatory mechanisms involved in the interactions between the virus and the target cells. In the present study, a new gene screened from the hepatoblastoma cell line HepG2 using suppression subtractive hybridization, referred to as lncRNA H...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2020.198104
更新日期：2020-10-02 00:00:00
abstract：:Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, is a causative agent of porcine enteric disease characterized by acute watery diarrhea and dehydration in sucking piglet. Similar to other coronaviruses, PEDV spike protein mediates its cell entry by binding to cellular receptors and induc...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.07.022
更新日期：2014-10-13 00:00:00
abstract：:A flow cytometric assay that measures binding of human T-lymphotropic virus type 1 (HTLV-1) virions to target cells was used to investigate the binding process and to screen for compounds affecting viral binding. Results showed that adenosine receptor type 2 antagonists effectively inhibit viral binding at concentrati...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(03)00048-0
更新日期：2003-05-01 00:00:00
abstract：:Recent evidence has accumulated which definitively shows that chemokine receptors CCR5 and CXCR4 play an essential role as coreceptors for human immunodeficiency virus type 1 (HIV-1) infection. Flow cytometric analysis permitted us to detect CD38, a surface marker of early differentiation, as well as activation of T c...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(00)00220-3
更新日期：2001-01-01 00:00:00
abstract：:Prion diseases are fatal neurodegenerative disorders by which the native cellular prion protein (PrPC) is misfolded into an accumulating, disease-associated isoform (PrPD). To improve the understanding of prion pathogenesis and develop effective treatments, it is essential to elucidate factors contributing to cellular...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2017.07.016
更新日期：2017-08-15 00:00:00
abstract：:The use of potyvirus-specific primers and subsequent application of the RACE procedure allowed the cloning of the 3' terminal 1088 nucleotides of the genomic RNA of the Taiwan isolate of sweetpotato latent virus (SPLV-T) and the 3' genomic 1085 nucleotides of a SPLV-like virus from China (SPLV-CH). The sequence of an ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(97)01456-1
更新日期：1997-05-01 00:00:00
abstract：:The purpose of this study was to generate complete genome sequences of Aravan (ARAV), Khujand (KHUV), Irkut (IRKV) and West Caucasian bat (WCBV) viruses, and to compare them with genomes of other lyssaviruses. We focused on RNA-dependent RNA-polymerase (L) and non-coding regions, because other genes of these viruses h...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.04.021
更新日期：2008-09-01 00:00:00
abstract：:The extensive nucleotide sequence heterogeneity among independent genotypes of wild polioviruses permits the systematic design of genotype-specific molecular reagents. We have prepared two sets of polymerase chain reaction (PCR) primer pairs specific for the genotype of wild poliovirus type 3 recently endemic to Mexic...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(92)90124-r
更新日期：1992-08-01 00:00:00
abstract：:Human recombinant alpha interferon, A/D, significantly reduced the replication and cell fusion induced by herpes simplex virus type 1 in monkey cells. Thin-section electron microscopy of interferon-treated monkey cells showed distinct assembly of nucleocapsids within the nucleus. Analysis of virus-specific proteins by...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90051-8
更新日期：1989-01-01 00:00:00
