Clathrin- and serine proteases-dependent uptake of porcine epidemic diarrhea virus into Vero cells.

Abstract:

:Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, is a causative agent of porcine enteric disease characterized by acute watery diarrhea and dehydration in sucking piglet. Similar to other coronaviruses, PEDV spike protein mediates its cell entry by binding to cellular receptors and inducing membrane fusion between viral envelopes and cellular membranes. However, the entry mechanism of PEDV is not studied. Here, we determined the entry mechanism of PEDV into Vero cells. Our data confirmed that PEDV entry followed clathrin-mediated endocytosis independence of caveolae-coated pit assembly. The internalized PEDV was co-localized with the clathrin-mediated endocytic marker, but not with the caveolae-mediated endocytic marker. In addition, cells treated with lysosomotropic agents and serine protease inhibitors were resistant to PEDV. Our data revealed that PEDV entry followed clathrin-mediated endocytosis and was dependent on a low pH and serine proteolysis for successful entry into cells.

journal_name

Virus Res

journal_title

Virus research

authors

Park JE,Cruz DJ,Shin HJ

doi

10.1016/j.virusres.2014.07.022

subject

Has Abstract

pub_date

2014-10-13 00:00:00

pages

21-9

eissn

0168-1702

issn

1872-7492

pii

S0168-1702(14)00300-1

journal_volume

191

pub_type

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