Abstract：
:To study the process of feline immunodeficiency virus (FIV) assembly, we examined the suitability of the vaccinia vector system to reproduce FIV particle formation. To this end, we constructed a recombinant vaccinia virus carrying the FIV gag gene. Biochemical and electron microscopy analyses of cells infected with this recombinant virus showed that the FIV Gag polyprotein self-assembled into lentivirus-like particles that were released into the culture medium. As a first step in the identification of molecular determinants in FIV Gag that are involved in virus assembly, we performed a site-directed mutagenesis analysis of the N-terminal matrix (MA) domain of the FIV Gag precursor. To this end, a series of amino acid substitutions and small in-frame deletions were introduced into the FIV MA and the mutated FIV gag gene constructs were expressed by means of the vaccinia system. Characterization of the assembly phenotype of these FIV Gag mutants led to the identification of amino acidic regions within the MA domain that are necessary for efficient transport of the Gag precursor to the plasma membrane and particle assembly. Our results reveal the role that the FIV MA plays in virus morphogenesis and contribute to the understanding of the assembly process in non-primate lentiviruses.
journal_name
Virus Resjournal_title
Virus researchauthors
Manrique ML,Celma CC,González SA,Affranchino JLdoi
10.1016/s0168-1702(01)00249-0subject
Has Abstractpub_date
2001-07-01 00:00:00pages
103-13issue
1eissn
0168-1702issn
1872-7492pii
S0168-1702(01)00249-0journal_volume
76pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract：:Plasmids containing the varicella zoster virus (VZV) open reading frames (ORFs) 61 and 62 were used in a transient co-transfection assay to test for trans-activation of the VZV and herpes simplex virus type 1 (HSV-1) thymidine kinase (tk) promoters. The trans-activating potential of the polypeptides encoded by these V...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(90)90013-2
更新日期：1990-01-01 00:00:00
abstract：:Tick-borne encephalitis (TBE) is a serious human neurological disease caused by TBE virus (TBEV). However, the mechanisms of TBEV-caused pathogenesis remain unclear. The endoplasmic reticulum (ER) stress response, also defined as the unfolded protein response (UPR), is an important conserved molecular signaling pathwa...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.10.012
更新日期：2013-12-26 00:00:00
abstract：:Severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) is recognized as one of the life-threatening viruses causing the most destructive pandemic in this century. The genesis of this virus is still unknown. To elucidate its molecular evolution and regulation of gene expression, the knowledge of codon usage is a...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2020.198113
更新日期：2020-10-15 00:00:00
abstract：:Influenza virus rgVac1sub-P0 (H5N1) (rgVac1-P0), in which a pair of dibasic amino acid residues was introduced at the cleavage site of the HA of a reassortant of H5N2 and H7N1 viruses of duck origin, was low pathogenic in chickens. Vac1sub-P3 (H5N1) (Vac1-P3) was selected as a highly pathogenic avian influenza virus b...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.02.001
更新日期：2013-05-01 00:00:00
abstract：:Porcine reproductive and respiratory syndrome virus (PRRSV) induces apoptosis in cultured cells and in infected tissues of pig, and the GP5 protein was reported to be responsible. To study the role of apoptosis during PRRSV infection, we established a subline of HeLa cells stably expressing the PRRSV GP5 protein under...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.03.001
更新日期：2004-08-01 00:00:00
abstract：:Cell lines were constructed which permanently express influenza virus-specific RNA. Two approaches were followed. C127 cells were transformed with bovine papilloma virus (BPV) vectors and the resulting cell lines were found to inhibit the replication of influenza virus at low multiplicity of infection (MOI 0.05). Howe...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90035-x
更新日期：1989-10-01 00:00:00
abstract：:The ultrastructure of the equine morbillivirus (EMV) which was implicated in the death of one human and fourteen horses in Queensland, Australia during September 1994 and a 36 year old man from Queensland in October 1995 is described. The ultrastructure of the virus and the intracellular virus-specific structures are ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(96)01307-x
更新日期：1996-07-01 00:00:00
abstract：:Although a significant progress has been achieved on dsRNA mediated anti-virus strategy development, there is still no effective means to control the virulent white spot syndrome virus (WSSV). Six double-stranded RNAs specific to different essential genes of WSSV (ie1, ie3, pol (DNA polymerase), rr2 (ribonucleotide re...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.06.018
更新日期：2009-10-01 00:00:00
abstract：:Certain neurotropic viruses can invade the nervous system of their hosts and spread in chains of synaptically connected neurons. Consequently, it is possible to identify entire hierarchically connected circuits within an animal. In this review, we discuss the use of neurotropic herpesviruses as neuronal tract tracers....
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2005.04.012
更新日期：2005-08-01 00:00:00
abstract：:Outbreaks caused by the highly pathogenic H5N1 avian influenza virus (A/H5N1) devastated the poultry industry in several countries and posed a significant pandemic threat. In addition to culling of infected poultry and vaccination, amantadine has been applied in poultry in some countries to control the spread of the v...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.11.032
更新日期：2017-01-15 00:00:00
abstract：:Subtype C is the most prevalent HIV-1 subtype globally and a common circulating recombinant form virus, CRF_BC, is predominant in China. It is unclear whether subtype C-based vaccines are capable of neutralizing CRF_BC viruses or can be evaluated using a neutralization assay based on CRF_BC pseudoviruses. Phylogenetic...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2010.09.012
更新日期：2011-01-01 00:00:00
abstract：:We have developed a system for the preparation of La Crosse virus (LAC) and Hantaan virus (HTN) pseudotypes using a murine leukemia virus vector. After concentration, the pseudotypes were present in quantities sufficient to analyze cell tropism and neutralization. Cells resistant to LAC could not be infected with the ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(99)00070-2
更新日期：1999-10-01 00:00:00
abstract：:A cell-free system was developed to study the replication of simian rotavirus SA11. The components of the system included (i) subviral particles prepared from infected cells to template the synthesis of viral RNA and (ii) an mRNA-dependent rabbit reticulocyte lysate to support protein synthesis. Based upon nuclease-se...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(86)90071-7
更新日期：1986-12-01 00:00:00
abstract：:Felid herpesvirus 1 (FHV-1) mutants were constructed using two-step Red-mediated recombination techniques based on a virulent full-length FHV-1 BAC clone. The individual mutant viruses generated were deficient in glycoprotein C (gC), glycoprotein E (gE), US3 serine/threonine protein kinase (PK), or both gE and thymidi...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.05.001
更新日期：2016-08-02 00:00:00
abstract：:Cytopathic bovine viral diarrhoea virus (cp BVDV) induces apoptosis in bovine cell cultures. This also seems to be a prominent feature in the pathogenesis of mucosal disease. To gain an insight into the molecular pathways of the cell alterations, the involvement of different members of the apoptotic cascade was analyz...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(02)00150-8
更新日期：2002-12-01 00:00:00
abstract：:Squirrel monkey retrovirus (SMRV) is a New World primate type D retrovirus. It was shown that SMRV-related sequences could be detected in another New World species, the skunk. It was further suggested that SMRV and an Old World primate type C retrovirus, baboon endogenous virus (BaEV), may have exchanged their env gen...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(03)00178-3
更新日期：2003-10-01 00:00:00
abstract：:Diagnostic methods allowing for rapid identification of pathogens are crucial for controlling and preventing dissemination after disease outbreaks as well as for use in surveillance programs. For arboviruses, detection of the presence of virus in their arthropod hosts is important for monitoring of viral activity and ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.07.019
更新日期：2012-10-01 00:00:00
abstract：:Hand, foot and mouth disease (HFMD) in humans is caused mainly by Enterovirus 71(EV71) and Coxsackievirus A16 (CVA16). EV71 is associated with severe HFMD cases but not CVA16. Use of IgM-capture enzyme-linked immunosorbent assay (ELISA) is important for the early diagnosis of EV71 infection, but cross-reactivity of th...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.04.015
更新日期：2013-09-01 00:00:00
abstract：:PCR amplification of proviral DNA extracted from peripheral blood lymphocytes of three Chinese pigs (Banna minipig inbreed (BMI), Wu-Zhi-Shan pig (WZSP) and Neijiang pig (NJP)), using primers corresponding to highly conserved regions of reverse transcriptase (RT) of pol gene and nucleocapsid sequence of gag gene. PCR ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.05.007
更新日期：2004-10-01 00:00:00
abstract：:Hepatitis C virus (HCV) is a modern-day pandemic; 2-3% of the world's population are thought to be infected with the virus and are subsequently at risk of developing end-stage liver diseases. The traditional standard of care (SOC) for HCV-infected patients has been limited to a regimen of pegylated-interferon alpha (p...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2012.09.007
更新日期：2012-12-01 00:00:00
abstract：:Several potexviruses (Family Alphaflexiviridae) have been reported infecting cassava (Manihot esculenta Crantz) in the Americas. They were isolated from severely diseased plants during the last 30-40 years and include: Cassava common mosaic virus (CsCMV), Cassava Caribbean mosaic virus (CsCaMV), Cassava Colombian symp...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2017.03.019
更新日期：2017-09-15 00:00:00
abstract：:Currently, pseudorabies virus (PRV) variant strains are outbreaking in China; these variants belong to genotype II PRV. The traditional Bartha-K61 vaccine has failed to provide complete protection against the emergent variant strains. Therefore, rapid attenuation of current epidemic strains is needed for effective PRV...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.09.004
更新日期：2016-10-02 00:00:00
abstract：:The mechanism by which hepatitis B virus (HBV) infection causes severe inflammatory liver diseases is multifactorial and related to interactions with cell signaling pathways and the ensuing inflammatory response. Activation of JAK/STAT/SOCS signaling is essential for the induction of cellular antiviral responses, cont...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.12.003
更新日期：2010-03-01 00:00:00
abstract：:A study of the evolutionary relationships of GLRaV-4,-5,-6 and -9, and two new Ampelovirus isolates (GLRaV-Pr and -De) related to grapevine leafroll disease was conducted based on molecular variability, positive selection analysis and maximum likelihood phylogenetic reconstructions. Sequences corresponding to the N-te...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.02.015
更新日期：2008-07-01 00:00:00
abstract：:Employing the yeast two-hybrid system, the Tat protein of the human immunodeficiency virus (HIV) was shown to interact with a region spanning the EGF-like repeats 1-6 of the mouse Notch1, the human Notch2 and the Drosophila Notch. This observation was confirmed in mammalian cells by demonstrating an interaction betwee...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2003.08.016
更新日期：2003-12-01 00:00:00
abstract：:Human recombinant alpha interferon, A/D, significantly reduced the replication and cell fusion induced by herpes simplex virus type 1 in monkey cells. Thin-section electron microscopy of interferon-treated monkey cells showed distinct assembly of nucleocapsids within the nucleus. Analysis of virus-specific proteins by...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90051-8
更新日期：1989-01-01 00:00:00
abstract：:A simple and sensitive method for the detection of measles virus genome was developed, amplifying the regions encoding the nucleocapsid (N) protein and hemagglutinin (H) protein of measles virus by reverse transcriptase-polymerase chain reaction (RT-PCR). We examined a variety of measles patients: 28 patients with nat...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(94)00074-m
更新日期：1995-01-01 00:00:00
abstract：:Consensus patterns of baculovirus sequences upstream from the translational initiation sites have been analyzed and a web tool, Local Alignment Promoter Predictor (LAPP), for the prediction of baculovirus promoter sequences has also been developed. Potential consensus sequences, i.e., TCATTGT, TCTTGTA, CTCGTAA, TCCATT...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2005.04.016
更新日期：2005-10-01 00:00:00
abstract：:Seneca Valley virus (SVV) is a novel Picornaviridae that is closely associated with porcine idiopathic vesicular disease (PIVD). Here, a novel SVV strain (CH-GX-01-2019) was detected and isolated from swine in Guangxi Province, China. The complete genomic sequence of CH-GX-01-2019 exhibited 93.3-98.9 % identify with o...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2020.198177
更新日期：2021-01-02 00:00:00
abstract：:Transcription of human immunodeficiency virus (HIV-1) is activated by viral Tat protein which regulates HIV-long terminal repeat (LTR) transcription and elongation. HIV-1 Tat protein is a substrate for the deacetylase activity of sirtuin 1 (SIRT1). Here we investigate the signaling pathway involved in Tat-induced HIV-...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.08.005
更新日期：2009-12-01 00:00:00
