Abstract：
:We have developed a system for the preparation of La Crosse virus (LAC) and Hantaan virus (HTN) pseudotypes using a murine leukemia virus vector. After concentration, the pseudotypes were present in quantities sufficient to analyze cell tropism and neutralization. Cells resistant to LAC could not be infected with the MLV (LAC) pseudotypes, and the pseudotypes were sensitive to neutralizing monoclonal antibodies prepared against LAC glycoproteins, as well as to inhibition by a soluble form of the virus cell-attachment protein, G1. Perhaps because of lower expression of the HTN glycoproteins at the cell surface, MLV (HTN) pseudotypes were present at lower titers. However, they were also sensitive to appropriate neutralizing antibodies. This pseudotype system will be useful for analysis of the entry process of the Bunyaviridae, and for neutralization studies with some Bunyaviruses whose high virulence normally requires specialized containment facilities.
journal_name
Virus Resjournal_title
Virus researchauthors
Ma M,Kersten DB,Kamrud KI,Wool-Lewis RJ,Schmaljohn C,González-Scarano Fdoi
10.1016/s0168-1702(99)00070-2subject
Has Abstractpub_date
1999-10-01 00:00:00pages
23-32issue
1eissn
0168-1702issn
1872-7492pii
S0168-1702(99)00070-2journal_volume
64pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract：:Human respiratory syncytial virus (HRSV) P protein, 241 amino acid long, is a structural homotetrameric phosphoprotein. Viral transcription and replication processes are dependent on functional P protein interactions inside viral ribonucleoprotein complexes (RNPs). Binding capacity to RNPs proteins and transcription a...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2007.11.013
更新日期：2008-03-01 00:00:00
abstract：:Entomopoxviruses are an important group of viruses infecting only insects. They belong to Poxviridae which infect both invertebrates and vertebrates, including humans. Protein kinases are known to have roles at virus morphogenesis, host selectivity, the regulation of cell division and apoptosis in some vertebrate poxv...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2018.02.007
更新日期：2018-03-15 00:00:00
abstract：:Transposable elements are valuable for somatic and germ-line transformation. However, long interspersed nuclear elements (LINEs) have not been used because of poor information on the transposition mechanism. We have developed a novel gene delivery system combining baculovirus AcNPV and two silkworm LINEs, SART1 and R1...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2007.03.014
更新日期：2007-07-01 00:00:00
abstract：:Genome organization and gene expression of Borna disease virus (BDV) are remarkable for the overlap of open reading frames, transcription units and transcription signals, readthrough of transcription termination signals, differential use of translation initiation codons, and exploitation of the cellular splicing machi...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.03.016
更新日期：2009-09-01 00:00:00
abstract：:DNAzyme is known to selectively cleave RNA at predetermined site. Transfection of Autographa californica nucleopolyhedrovirus (AcNPV) infected Sf9 cells with serine/threonine kinase (pk1) mRNA specific DNAzymes, DZ1 and DZ2 to cleave the viral coded (pk1) mRNA in between 87th and 88th, and 250th and 251st nucleotide, ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.02.002
更新日期：2008-07-01 00:00:00
abstract：:HIV-1 detected among female sex workers in Calcutta, India was characterized in respect to env and nef genes. A total of 39 HIV-1 seropositive samples were used in the study. Phylogenetic analysis of the nucleotide sequences of respective regions showed that 22 out of 39 samples (56.4%) were infected with subtype C wi...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2007.06.013
更新日期：2007-12-01 00:00:00
abstract：:Human recombinant alpha interferon, A/D, significantly reduced the replication and cell fusion induced by herpes simplex virus type 1 in monkey cells. Thin-section electron microscopy of interferon-treated monkey cells showed distinct assembly of nucleocapsids within the nucleus. Analysis of virus-specific proteins by...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90051-8
更新日期：1989-01-01 00:00:00
abstract：:It has recently been reported that the xenotropic murine leukemia virus-related virus (XMRV) derives from a laboratory recombinant. However, sequences with characteristics of the 5' half of XMRV (termed PreXMRV-2) have been identified in several laboratory mouse genomes and cell lines suggesting parts of the XMRV geno...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.06.026
更新日期：2012-10-01 00:00:00
abstract：:The A antigen gene from a very virulent strain of Marek's disease virus, RB-1B, has been cloned and the nucleotide sequence determined. The predicted amino acid sequence showed 99% identity to that determined for the MDV GA A antigen (Coussens and Velicer, J. Virol. 62, 2373-2379, 1988) over all but the carboxy-termin...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90094-4
更新日期：1989-04-01 00:00:00
abstract：:The genomes of eight hepatitis A virus (HAV) strains originating from far distant geographic regions such as Europe, North Africa, Middle and North America, Australia and The People's Republic of China were compared by RNase T1 oligonucleotide mapping. For this purpose, the viruses were propagated in cell cultures and...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(85)90020-6
更新日期：1985-12-01 00:00:00
abstract：:We previously prepared neutralizing monoclonal antibody (MAb)-resistant (mar) mutant viruses using a laboratory strain feline infectious peritonitis virus (FIPV) 79-1146 (Kida et al., 1999). Mar mutant viruses are mutated several amino acids of the neutralizing epitope of Spike protein, compared with the parent strain...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2010.12.020
更新日期：2011-03-01 00:00:00
abstract：:A comprehensive phylogenetic study based on the hemagglutinin (H) protein of all known African measles virus (MV) isolates is presented. The study includes 64 new H gene sequences from Ghana. Nigeria and South Africa as well as viruses from Zambia and The Gambia for which only incomplete sequencing data were available...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(99)00072-6
更新日期：1999-07-01 00:00:00
abstract：:The human poliovirus receptor (PVR) is a cell surface protein with a multitude of functions in human biology. PVR was initially identified as the receptor for the human poliovirus and recent discoveries have given a greater insight into both its morphology and its function. Alternative splicing of the PVR gene results...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2017.09.001
更新日期：2017-10-15 00:00:00
abstract：:Recombinant clones containing all or portions of the bovine papillomavirus (BPV) upstream regulatory region (URR) were constructed. When analyzed by polyacrylamide gel electrophoresis, the cloned URR sequences exhibited electrophoretic properties characteristic of DNA containing bend sites. Two distinct bend centers w...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90083-x
更新日期：1989-05-01 00:00:00
abstract：:Infectious salmon anaemia virus (ISAV) is a piscine orthomyxovirus causing a serious disease in farmed Atlantic salmon (Salmo salar L.). The virus surface glycoprotein hemagglutinin-esterase (HE) is responsible for both viral attachment and release. Similarity to bovine and porcine torovirus hemagglutinin-esterase (BT...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2010.03.020
更新日期：2010-08-01 00:00:00
abstract：:In this study, we tried to identify dengue virus-specific CD4(+) T-cell epitopes, which can induce PBMC (peripheral blood mononuclear cells) isolated from DF convalescent patients (dengue virus type 1 infection) to secrete IFN-gamma. PBMC of DF convalescent patients were stimulated in vitro with dengue virus-derived p...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2007.10.010
更新日期：2008-03-01 00:00:00
abstract：:All lepidopteran baculovirus genomes sequenced to date encode a viral fibroblast growth factor homolog (vFGF). Recently, we generated a Bombyx mori nucleopolyhedrovirus (BmNPV) mutant lacking functional vfgf and found that BmNPV vfgf contributes to virus virulence in B. mori larvae. However, the steps at which BmNPV v...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.06.002
更新日期：2008-10-01 00:00:00
abstract：:Lepidopteran nucleopolyhedroviruses have distinct viral tissue tropisms in host larvae. We previously identified the Bm8 gene of Bombyx mori nucleopolyhedrovirus (BmNPV), the product of which inhibits viral propagation in the middle silk gland (MSG). However, it is unknown whether this inhibitory function of the Bm8 p...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2018.03.011
更新日期：2018-04-02 00:00:00
abstract：:Viruses of the genus 'Norwalk-like viruses' (NLVs) detected in humans have been genetically classified into two major genetic groups, genogroups I and II (GI and GII), which together are made up of at least 14 genetic subgroups. However, a comparable classification of NLVs in other species remains to be carried out. W...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(02)00107-7
更新日期：2002-08-01 00:00:00
abstract：:To examine the regulatory properties of feline immunodeficiency virus (FIV) long terminal repeat (LTR) integrated into host chromatin, Crandell feline kidney cells were stably transfected with the FIV LTR that directs the bacterial chloramphenicol acetyltransferase (CAT) gene. Using these cells, we examined the effect...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(96)01284-1
更新日期：1996-04-01 00:00:00
abstract：:We describe a rapid method for genetic characterisation of influenza virus genes using reverse transcription and amplification by polymerase chain reaction (RT/PCR) of all virus segments simultaneously (multiplex RT/PCR) using primers based on the conserved terminal sequences. The product has been shown to be suitable...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(94)90087-6
更新日期：1994-06-01 00:00:00
abstract：:Influenza B viruses isolated in southern Africa during the period from 1998 to 2001 were analysed by sequence analysis of the viral haemagglutinin HA1 subunit and the phylogenetic relationships were determined. Influenza B activity varied considerably in South Africa during the 4-year study period with no activity det...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.02.014
更新日期：2004-07-01 00:00:00
abstract：:Nervous necrosis virus (NNV) belongs to the genus Betanodavirus (Nodaviridae). It is highly pathogenic to various marine fishes. Here, we investigated the antigenicity changes of cultured NNV suspensions during 14days of dialyses using a dialysis tube at 1.4×10(4) molecular weight cut off (MWCO) in three different buf...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.07.002
更新日期：2016-09-02 00:00:00
abstract：:Viruses and virus-like particles (VLPs) of insect parasitoids modify host-parasite interactions. The Drosophila wasp, Leptopilina heterotoma, produce 300 nm spiked VLPs that bind to the host's blood cells via surface projections. L. heterotoma is a generalist wasp that attacks over a dozen Drosophila species. Oviposit...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2011.06.005
更新日期：2011-09-01 00:00:00
abstract：:Squirrel monkey retrovirus (SMRV) is a New World primate type D retrovirus. It was shown that SMRV-related sequences could be detected in another New World species, the skunk. It was further suggested that SMRV and an Old World primate type C retrovirus, baboon endogenous virus (BaEV), may have exchanged their env gen...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(03)00178-3
更新日期：2003-10-01 00:00:00
abstract：:Vesicular stomatitis virus (VSV) is a prototypic nonsegmented negative-strand RNA virus. VSV's broad cell tropism makes it a popular model virus for many basic research applications. In addition, a lack of preexisting human immunity against VSV, inherent oncotropism and other features make VSV a widely used platform f...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2013.06.003
更新日期：2013-09-01 00:00:00
abstract：:A recent study has shown that the reovirus lambda1 protein can unwind double-stranded nucleic acid molecules, a process energetically coupled to the hydrolysis of nucleoside 5'-triphosphates. In the present study, it was demonstrated that lambda1, expressed as a fusion protein with the Escherichia coli maltose-binding...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(97)00092-0
更新日期：1997-10-01 00:00:00
abstract：:Mouse Hepatitis Virus (MHV) is a single-stranded positive sense RNA virus with the ability to promote acute and chronic diseases in mice. The MHV spike protein (S) is a major virulence determinant which in addition to binding to cellular receptors to mediate cell entry and facilitate virus spread to adjacent cells by ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.05.023
更新日期：2014-08-30 00:00:00
abstract：:We have used vesicular stomatitis virus (VSV) to determine the cost of antiserum resistance during escape from a polyclonal immune response. Replication of VSV in the presence of polyclonal antiserum resulted in the selection of antibody-escape mutants, as shown by increased fitness in the presence of antiserum and by...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.06.007
更新日期：2005-01-01 00:00:00
abstract：:Sixty-six human enterovirus serotypes have been described using antibody neutralization, with antigenic variants defined within several serotypes. Despite the availability of sequence data for numerous enteroviruses, the molecular basis of serotype is unknown. Previous studies by others have identified four major phyl...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(98)00101-4
更新日期：1998-11-01 00:00:00
