Abstract：
:Currently, pseudorabies virus (PRV) variant strains are outbreaking in China; these variants belong to genotype II PRV. The traditional Bartha-K61 vaccine has failed to provide complete protection against the emergent variant strains. Therefore, rapid attenuation of current epidemic strains is needed for effective PRV control. In this study, we report a rapid method for editing the PRV genome using the CRISPR-Cas9 system. We developed a triple gE/gI/TK gene-inactivated HeN1 PRV strain, because mice were more susceptible to PRV infection, we then evaluated the attenuation of PRV in the mice and demonstrated that modified PRV was fully attenuated. Furthermore, the attenuated strain also induced immune protection in response to a parental PRV challenge. Overall, we showed that PRVs can be rapidly attenuated using CRISPR-Cas9 technology, which will be critical for PRV control, especially when new variant PRV strains emerge.
journal_name
Virus Resjournal_title
Virus researchauthors
Tang YD,Liu JT,Wang TY,An TQ,Sun MX,Wang SJ,Fang QQ,Hou LL,Tian ZJ,Cai XHdoi
10.1016/j.virusres.2016.09.004subject
Has Abstractpub_date
2016-10-02 00:00:00pages
33-39eissn
0168-1702issn
1872-7492pii
S0168-1702(16)30186-1journal_volume
225pub_type
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