Abstract：
:In the early 1970s, the Chinese Equine Infectious Anemia Virus (EIAV) vaccine, EIAV(DLA), was developed through successive passages of a wild-type virulent virus (EIAV(L)) in donkeys in vivo and then in donkey macrophages in vitro. EIAV attenuation and cell tropism adaptation are associated with changes in both envelope and long terminal repeat (LTR). However, specific LTR changes during Chinese EIAV attenuation have not been demonstrated. In this study, we compared LTR sequences from both virulent and attenuated EIAV strains and documented the diversities of LTR sequence from in vivo and in vitro infections. We found that EIAV LTRs of virulent strains were homologous, while EIAV vaccine have variable LTRs. Interestingly, experimental inoculation of EIAV(DLA) into a horse resulted in a restriction of the LTR variation. Furthermore, LTRs from EIAV(DLA) showed higher Tat transactivated activity than LTRs from virulent strains. By using chimeric clones of wild-type LTR and vaccine LTR, the main difference of activity was mapped to the changes of R region, rather than U3 region.
journal_name
Virus Resjournal_title
Virus researchauthors
Zhou T,Yuan XF,Hou SH,Tu YB,Peng JM,Wen JX,Qiu HJ,Wu DL,Chen HC,Wang XJ,Tong GZdoi
10.1016/j.virusres.2007.04.005subject
Has Abstractpub_date
2007-09-01 00:00:00pages
58-64issue
1-2eissn
0168-1702issn
1872-7492pii
S0168-1702(07)00123-2journal_volume
128pub_type
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