当前位置： SCI文献检索 > VIRUS RESEARCH期刊下所有文献 > A modified viral satellite DNA-based gene silencing vector is effective in association with heterologous begomoviruses.

A modified viral satellite DNA-based gene silencing vector is effective in association with heterologous begomoviruses.

Abstract：

:We have previously reported effective gene silencing of a transgene and endogenous plant genes in tobacco and tomato plants using a modified viral satellite DNA associated with Tomato yellow leaf curl China virus (TYLCCNV). In this study, we constructed a similar gene silencing vector (DNADeltaC12beta) based on the satellite DNAbeta associated with Tobacco curly shoot virus (TbCSV) by replacing its betaC1 gene with a multiple cloning site. Strong and stable silencing of cognate genes was achieved when this vector, carrying a fragment of the green fluorescent protein (GFP) transgene or a sulfur (Su) endogenous gene encoding one unit of the chloroplast enzyme magnesium chelatase required for chlorophyll II production, was co-agroinoculated with TbCSV used as a helper virus. GFP silenced transgenic Nicotiana benthamiana plants appear red under UV illumination due to loss of green fluorescence, while the Su silenced plants appear white as a result of failure to synthesize chlorophyll. Our results show that the efficiency of Su silencing is independent of the insert orientation in both N. benthamiana and N. glutinosa plants. Most significant however, is the observation that in association with heterologous begomoviruses, such as TYLCCNV or Malvastrum yellow vein virus, the DNADeltaC12beta vector could still effectively induce transgene and endogenous gene silencing in tobacco plants. These observations suggest that the modified viral satellite DNA vector can be applied as a reverse genetics tool for the study, analysis and discovery of gene function in more plants.

journal_name

Virus Res

journal_title

Virus research

authors

Qian Y,Mugiira RB,Zhou X

doi

10.1016/j.virusres.2005.12.003

subject

Has Abstract

pub_date

2006-06-01 00:00:00

pages

136-42

issue

1-2

eissn

0168-1702

issn

1872-7492

pii

S0168-1702(05)00385-0

journal_volume

118

pub_type

杂志文章

相关文献

VIRUS RESEARCH文献大全
  • The fibroblast growth factor homolog of Bombyx mori nucleopolyhedrovirus enhances systemic virus propagation in B. mori larvae.

    abstract：:All lepidopteran baculovirus genomes sequenced to date encode a viral fibroblast growth factor homolog (vFGF). Recently, we generated a Bombyx mori nucleopolyhedrovirus (BmNPV) mutant lacking functional vfgf and found that BmNPV vfgf contributes to virus virulence in B. mori larvae. However, the steps at which BmNPV v...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2008.06.002

    authors： Katsuma S,Horie S,Shimada T

    更新日期：2008-10-01 00:00:00

  • Ozone therapy in COVID-19: A narrative review.

    abstract：:The main objective of this narrative review is to describe the available evidence on the possible antiviral activity of ozone in patients with COVID-19 and its therapeutic applicability through hospital protocols. Amongst different possible therapies for SARS-CoV-2 pneumonia, ozone therapy seems to have an immunologic...

    journal_title：Virus research

    pub_type： 杂志文章,评审

    doi：10.1016/j.virusres.2020.198207

    authors： Cattel F,Giordano S,Bertiond C,Lupia T,Corcione S,Scaldaferri M,Angelone L,De Rosa FG

    更新日期：2021-01-02 00:00:00

  • Major amino acid sequence variants of viral vascular endothelial growth factor are functionally equivalent during Orf virus infection of sheep skin.

    abstract：:Orf virus infection causes a contagious pustular dermatitis characterized by extensive vascular changes that have been linked to a virally encoded vascular endothelial growth factor (VEGF). The VEGF genes of different strains of orf virus can vary extensively in amino acid sequence. Functional analyses of two major va...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2007.04.018

    authors： Wise LM,Savory LJ,Dryden NH,Whelan EM,Fleming SB,Mercer AA

    更新日期：2007-09-01 00:00:00

  • Escape mutants of pandemic influenza A/H1N1 2009 virus: variations in antigenic specificity and receptor affinity of the hemagglutinin.

    abstract：:A panel of 6 neutralizing monoclonal antibodies (MAbs) raised against A/Moscow/IIV01/2009 (H1N1) virus isolated during the 2009 pandemic was used for the selection of 26 escape mutants. The mutants were characterized in immune cross-reactions with the panel of MAbs. The sequencing of the mutant HA genes revealed 5 ami...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2012.03.003

    authors： Rudneva I,Ignatieva A,Timofeeva T,Shilov A,Kushch A,Masalova O,Klimova R,Bovin N,Mochalova L,Kaverin N

    更新日期：2012-06-01 00:00:00

  • Recombination analyses between two strains of porcine reproductive and respiratory syndrome virus in vivo.

    abstract：:Porcine reproductive and respiratory syndrome virus (PRRSV) is characteristic of genetically extensive variation. In this study, five SPF pigs were co-infected with two strains of PRRSV (JXwn06-81c and HB-1/3.9c), and 352 viruses were cloned by plaque assay from the sera of the infected pigs on days 3, 5, 7, 10, 14, 2...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2010.12.003

    authors： Liu D,Zhou R,Zhang J,Zhou L,Jiang Q,Guo X,Ge X,Yang H

    更新日期：2011-02-01 00:00:00

  • Cleavage of the respiratory syncytial virus fusion protein is required for its surface expression: role of furin.

    abstract：:The fusion (F) glycoprotein of respiratory syncytial virus (RSV) is synthesized as a nonfusogenic precursor protein (F(0)), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F(1) and F(2) subunits. In the present study, soluble secreted human furin produced by a recombin...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/s0168-1702(00)00149-0

    authors： Bolt G,Pedersen LO,Birkeslund HH

    更新日期：2000-06-01 00:00:00

  • KSHV ORF67 encoded lytic protein localizes on the nuclear membrane and alters emerin distribution.

    abstract：:p29, a newly identified Kaposi's sarcoma-associated herpesvirus (KSHV) protein, is the product of ORF67, the positional homolog of the conserved herpesvirus protein UL34. Like its homologues in other herpesviruses, p29 is expressed early during viral lytic cycle, and is localized on the nuclear rim. Upon chemical indu...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2013.04.001

    authors： Farina A,Santarelli R,Bloise R,Gonnella R,Granato M,Bei R,Modesti A,Cirone M,Bengtsson L,Angeloni A,Faggioni A

    更新日期：2013-08-01 00:00:00

  • Localisation of the temperature-sensitive defect in the nucleoprotein of an influenza A/FPV/Rostock/34 virus.

    abstract：:The nucleotide sequences of the nucleoprotein (NP) genes of fowl plague virus (FPV) and of a temperature-sensitive (ts) mutant (ts81) derived therefrom have been determined. The ts81-NP nucleotide sequence possesses a single nucleotide substitution in comparison to the wild type. This causes an amino acid exchange at ...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(89)90058-0

    authors： Mandler J,Scholtissek C

    更新日期：1989-02-01 00:00:00

  • Beta-actin interacts with the E2 protein and is involved in the early replication of classical swine fever virus.

    abstract：:The E2 glycoprotein of classical swine fever virus (CSFV) is involved in viral infection and induction of neutralizing antibodies. Little is known about how E2 interacts with host cells. To understand the cellular factors involved in viral replication cycle, E2 was used as bait in yeast two-hybrid screens, resulting i...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2013.10.016

    authors： He F,Ling L,Liao Y,Li S,Han W,Zhao B,Sun Y,Qiu HJ

    更新日期：2014-01-22 00:00:00

  • Strand-specific compositional asymmetries in double-stranded DNA viruses.

    abstract：:Analysis of 22 complete sequences of double-stranded DNA viruses reveals striking compositional asymmetries between leading and lagging, and between transcribed and non-transcribed strands. In all bi-directionally replicated genomes analyzed, the observed leading strand GC skew (measuring relative excess of guanines v...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/s0168-1702(98)00139-7

    authors： Grigoriev A

    更新日期：1999-03-01 00:00:00

  • Molecular mechanisms of influenza virus resistance to neuraminidase inhibitors.

    abstract：:A wide use of inhibitors of influenza virus neuraminidase (NAIs) to control influenza in humans demands a better understanding of the mechanisms involved in the resistance emergence. In vitro studies demonstrate that both neuraminidase (NA) and hemagglutinin (HA) influence virus susceptibility to NAIs. Drug resistance...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2004.02.034

    authors： Gubareva LV

    更新日期：2004-07-01 00:00:00

  • Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus.

    abstract：:We have previously described the efficient homologous recombination system between 5' subgenomic RNA3a (sgRNA3a) and genomic RNA3 of Brome mosaic virus (BMV) in barley protoplasts (Sztuba-Solińska et al., 2011a). Here, we demonstrated that sequence alterations in the coat protein (CP)-binding cis-acting RNA motifs, th...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2012.10.001

    authors： Sztuba-Solińska J,Fanning SW,Horn JR,Bujarski JJ

    更新日期：2012-12-01 00:00:00

  • Regulation of HIV-1 transcription by NF-IL6 in activated Jurkat T cells.

    abstract：:To examine the mechanism of HIV-1 regulation by NF-IL6 in activated human cells, we selected a Jurkat cell line that did not contain endogenous NF-IL6. In this cellular environment, we evaluated the effect of exogenous NF-IL6 on transcription mediated by native and deleted LTR sequences. In Jurkat cells stimulated wit...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/s0168-1702(02)00110-7

    authors： Buckner AE,Tesmer VM,Bina M

    更新日期：2002-10-01 00:00:00

  • F gene recombination between genotype II and VII Newcastle disease virus.

    abstract：:A velogenic Newcastle disease virus (NDV) strain, designated as SRZ03, was isolated from an egg layer flock with NDV vaccine immunization failure in China in 2003. Recombination was found in the F gene of SRZ03. Complete genome sequences analysis indicated that the N-terminal of SRZ03 F gene originated from a genotype...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2007.10.001

    authors： Qin Z,Sun L,Ma B,Cui Z,Zhu Y,Kitamura Y,Liu W

    更新日期：2008-02-01 00:00:00

  • Identification of the interaction domains of white spot syndrome virus envelope proteins VP28 and VP24.

    abstract：:VP28 and VP24 are two major envelope proteins of white spot syndrome virus (WSSV). The direct interaction between VP28 and VP24 has been described in previous studies. In this study, we confirmed this interaction and mapped the interaction domains of VP28 and VP24 by constructing a series of deletion mutants. By co-im...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2015.01.017

    authors： Li Z,Chen W,Xu L,Li F,Yang F

    更新日期：2015-03-16 00:00:00

  • Porcine circovirus type 2 and its associated diseases in Korea.

    abstract：:This review describes the characterization of porcine circovirus type 2 (PCV2), the field situation of porcine circovirus-associated disease (PCVAD) and the PCV2 vaccine in Korea. PCVAD has been considered the most devastating disease in Korean livestock history since its first outbreak in 1999. Postweaning multisyste...

    journal_title：Virus research

    pub_type： 杂志文章,评审

    doi：10.1016/j.virusres.2011.10.013

    authors： Chae C

    更新日期：2012-03-01 00:00:00

  • A novel, highly divergent ssDNA virus identified in Brazil infecting apple, pear and grapevine.

    abstract：:Fruit trees of temperate and tropical climates are of great economical importance worldwide and several viruses have been reported affecting their productivity and longevity. Fruit trees of different Brazilian regions displaying virus-like symptoms were evaluated for infection by circular DNA viruses. Seventy-four fru...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2015.07.005

    authors： Basso MF,da Silva JC,Fajardo TV,Fontes EP,Zerbini FM

    更新日期：2015-12-02 00:00:00

  • Some epidemiological approaches to the control of aphid-borne virus diseases in seed potato crops in northern Europe.

    abstract：:An account is given of progress during the last 30 years in knowledge of the epidemiology of diseases caused by aphid-borne viruses in seed potato production areas of northern Europe. During this period, Potato leafroll virus (PLRV) and strains of Potato virus Y (PVY(O), PVY(N)) were consistently the most prevalent an...

    journal_title：Virus research

    pub_type： 杂志文章,评审

    doi：10.1016/s0168-1702(00)00186-6

    authors： Robert Y,Woodford JA,Ducray-Bourdin DG

    更新日期：2000-11-01 00:00:00

  • Antigenic diversity of Akabane virus detected by monoclonal antibodies.

    abstract：:The antigenic properties of 21 Japanese field isolates and two Australian strains of Akabane (AKA) virus (Simbu serogroup, bunyavirus) isolated from 1959 to 1990 were compared by enzyme-linked immunosorbent assay (ELISA), plaque-reduction neutralization (PRNT) and hemagglutination inhibition (HI) tests using monoclona...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/s0168-1702(96)01415-3

    authors： Akashi H,Inaba Y

    更新日期：1997-02-01 00:00:00

  • Expression of the mutagenic peptide of herpes simplex virus type 1 in virus-infected cells.

    abstract：:A fragment of DNA from within the minimum transforming region (mtr-1) of herpes simplex virus type 1 (HSV-1) is known to raise the mutation frequency of cells. This activity has been attributed to a viral protein whose properties are largely unknown. Antiserum was raised to a synthetic peptide of a predicted amino aci...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(94)90093-0

    authors： Das CM,Zhang S,Shillitoe EJ

    更新日期：1994-11-01 00:00:00

  • Porcine adenoviruses types 1, 2 and 3 have short and simple early E-3 regions.

    abstract：:The nucleotide sequence of the E-3 region genes, the hexon associated protein pVIII genes, and part of the fiber genes coding for the N-terminal tail regions, of porcine adenovirus (PAV) types 1 and 2 were determined. The sizes of the E-3 regions were found to be 1162 and 1222 bp, respectively. The five open reading f...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(96)01336-6

    authors： Reddy PS,Idamakanti N,Derbyshire JB,Nagy E

    更新日期：1996-08-01 00:00:00

  • Recent emergence of the Arctic rabies virus lineage.

    abstract：:The rabies viruses that circulate in Arctic countries and in much of northern and central Asia are phylogenetically closely related and collectively referred to as the Arctic/Arctic-like (AL) lineage. The emergence and spread of this lineage is of significant interest given that rabies remains a serious zoonotic disea...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2011.10.026

    authors： Nadin-Davis SA,Sheen M,Wandeler AI

    更新日期：2012-01-01 00:00:00

  • Conflicting selection pressures target the NS3 protein in hepatitis C virus genotypes 1a and 1b.

    abstract：:Analysis of complete polyprotein-encoding sequences of the two most prevalent genotypes of hepatitis C virus (HCV-1a and HCV-1b) revealed evidence of abundant, slightly deleterious nonsynonymous variants subject to ongoing purifying selection. In the case of both HCV-1a and HCV-1b, the NS3 protein demonstrated a high ...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2009.11.001

    authors： Irausquin SJ,Hughes AL

    更新日期：2010-02-01 00:00:00

  • Maturation efficiency of viral glycoproteins in the ER impacts the production of influenza A virus.

    abstract：:We have studied which steps are enhanced in the infectious cycle of influenza A virus in Madin-Darby canine kidney (MDCK) cells, a cell line investigated for use in the production of an influenza vaccine because of its ability to yield high levels of virus. We have confirmed that MDCK had the highest production levels...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2008.04.028

    authors： Ueda M,Yamate M,Du A,Daidoji T,Okuno Y,Ikuta K,Nakaya T

    更新日期：2008-09-01 00:00:00

  • In vitro characterization of felid herpesvirus 1 (FHV-1) mutants generated by recombineering in a recombinant BAC vector.

    abstract：:Felid herpesvirus 1 (FHV-1) mutants were constructed using two-step Red-mediated recombination techniques based on a virulent full-length FHV-1 BAC clone. The individual mutant viruses generated were deficient in glycoprotein C (gC), glycoprotein E (gE), US3 serine/threonine protein kinase (PK), or both gE and thymidi...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2016.05.001

    authors： Tai SH,Holz C,Engstrom MD,Cheng HH,Maes RK

    更新日期：2016-08-02 00:00:00

  • Avian influenza and Newcastle disease viruses from northern pintail in Japan: isolation, characterization and inter-annual comparisons during 2006-2008.

    abstract：:Since wild ducks constitute a vital element in the epizootiology of avian influenza viruses (AIVs) as well as avian paramyxoviruses (APMVs) and play a key role in the ecology and inter-species transmission of these viruses, it is crucial to elucidate the diversity and prevalence of these viruses within these bird popu...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2009.02.018

    authors： Jahangir A,Ruenphet S,Ueda S,Ueno Y,Shoham D,Shindo J,Okamura M,Nakamura M,Takehara K

    更新日期：2009-07-01 00:00:00

  • Post-translational modification of baculovirus-encoded proteins.

    abstract：:Post-translational modifications (PTMs) are the chemical modifications of proteins after translation, and are very important to guarantee the proper biological functions of these proteins. Baculoviruses are pathogenic viruses that infect invertebrates and have large circular double-stranded DNA genomes. Many proteins ...

    journal_title：Virus research

    pub_type： 杂志文章,评审

    doi：10.1016/j.virusres.2020.197865

    authors： Chen N,Kong X,Zhao S,Xiaofeng W

    更新日期：2020-04-02 00:00:00

  • Selection and characterization of peptides specifically binding to HIV-1 psi (psi) RNA.

    abstract：:The packaging of HIV genomic RNA is mediated by a specific interaction between a nucleocapsid (NC) protein and packaging signal (psi, psi) RNA sequence. However, this interaction can be inhibited by the presence of peptides or proteins that specifically bind to the psi sequence. The 125-base-long psi RNA comprises a s...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2004.05.012

    authors： Park MY,Kwon J,Lee S,You J,Myung H

    更新日期：2004-11-01 00:00:00

  • A century of plant virus management in the Salinas valley of California, 'East of Eden'.

    abstract：:The mild climate of the Salinas Valley, CA lends itself well to a diverse agricultural industry. However, the diversity of weeds, crops and insect and fungal vectors also provide favorable conditions for plant virus disease development. This paper considers the incidence and management of several plant viruses that ha...

    journal_title：Virus research

    pub_type： 杂志文章,评审

    doi：10.1016/s0168-1702(00)00196-9

    authors： Wisler GC,Duffus JE

    更新日期：2000-11-01 00:00:00

  • Amino acid at position 95 of the matrix protein is a cytopathic determinant of rabies virus.

    abstract：:The molecular mechanism involved in cytopathogenicity of rabies virus has not been fully elucidated yet. A fixed rabies virus Nishigahara strain does not induce clear cytopathic effect (CPE) in mouse neuroblastoma (NA) cells, whereas Ni-CE strain, which was established after 100 passages of Nishigahara strain in chick...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2008.05.011

    authors： Mita T,Shimizu K,Ito N,Yamada K,Ito Y,Sugiyama M,Minamoto N

    更新日期：2008-10-01 00:00:00