Abstract:
:A wide use of inhibitors of influenza virus neuraminidase (NAIs) to control influenza in humans demands a better understanding of the mechanisms involved in the resistance emergence. In vitro studies demonstrate that both neuraminidase (NA) and hemagglutinin (HA) influence virus susceptibility to NAIs. Drug resistance conferred due to changes in the NA could be monitored in the NA inhibition assays. Zanamivir-selected viruses acquired the NA substitutions at residues 119 and 292; oseltamivir-selected--at 274 and 292; peramivir-selected--at 292; and A-315675-selected--at 119. The HA binding efficiency and therefore susceptibility to NAIs are affected by the amino acids forming the HA receptor-binding site, the location and number of oligosaccharide chains, and structure of the neuraminic acid-containing cellular receptors. The lack of suitable cell culture-based assays hampers the assessment of virus susceptibility in humans. Emergence of the viruses with the NAI-induced substitutions in the NA is uncommon in drug-treated humans, however a compromised state of the immune system promotes emergence of drug resistance. In vivo, the zanamivir-selected mutant contained a substitution at 152 (B/NA); the oseltamivir-selected mutants-at residues 119 (A/N2), 198 (B/NA), 274 (A/N1), and 292 (A/N2). Substitutions in the NA were often accompanied by impairment of virus infectivity and virulence in animal models. Because of complexity of mechanisms of virus resistance, further analysis of the viruses recovered from the drug-treated humans is warranted.
journal_name
Virus Resjournal_title
Virus researchauthors
Gubareva LVdoi
10.1016/j.virusres.2004.02.034subject
Has Abstractpub_date
2004-07-01 00:00:00pages
199-203issue
1-2eissn
0168-1702issn
1872-7492pii
S0168170204001339journal_volume
103pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract::We selected Chinese hamster ovary (CHO) cell-adapted strains of human enterovirus 71 (HEV71) belonging to sub-genogroups B5 (HEV71-B5) and C2 (HEV71-C2) by serial passage in CHO cells at a high multiplicity of infection. During the course of CHO cell passage, virus growth improved significantly, with increasing virus ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.02.009
更新日期:2012-05-01 00:00:00
abstract::A double-stranded (ds) RNA, approximately 9.5kb in size; was identified in the MVC86 isolate of Valsa ceratosperma. Complete sequence of the dsRNA revealed a 9543-bp segment (excluding the 3' poly-A tail) that is predicted to encode a single large protein (P330). P330 has 63%, 49%, and 55% amino acid sequence identiti...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.02.008
更新日期:2012-05-01 00:00:00
abstract::Phylogenetic analysis has been extensively used as a tool for the reconstruction of epidemiological relations for research or for forensic purposes. It was our objective to assess the sensitivity of different phylogenetic methods and various phylogenetic programs to reconstruct epidemiological links among HIV-1 infect...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.03.002
更新日期:2012-06-01 00:00:00
abstract::Herpes simplex virus glycoprotein B (HSV gB) is essential for penetration of virus into cells, for cell-to-cell spread of virus, and for cell-cell fusion. Every member of the family Herpesviridae has a gB homolog, underlining its importance. The antigenic structure of gB has been studied extensively, but little is kno...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(98)00030-6
更新日期:1998-05-01 00:00:00
abstract::The BKRF2, BKRF3 and BKRF4 genes of Epstein-Barr virus (EBV) are located close together in the viral genome, which encode glycoprotein L, uracil-DNA glycosylase and a tegument protein, respectively. Here, we demonstrate that the BKRF2 gene behaves as a true-late lytic gene, whereas the BKRF3 and BKRF4 genes belong to ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2018.08.005
更新日期:2018-09-02 00:00:00
abstract::Oryctes rhinoceros nudivirus (OrNV) has been an effective biocontrol agent against the insect pest Oryctes rhinoceros (Coleoptera: Scarabaeidae) for decades, but there is evidence that resistance could be evolving in some host populations. We detected OrNV infection in O. rhinoceros from Solomon Islands and used Oxfor...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2020.197864
更新日期:2020-03-01 00:00:00
abstract::An insect High-Five cell line was generated constitutively and stably expressing the VP2 protein of rotavirus RF strain leading to the formation of core-like particles. The integration of the VP2 gene was confirmed by PCR, Real-time PCR and Southern blot, and the protein expression was confirmed by Western blot and im...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.12.015
更新日期:2013-03-01 00:00:00
abstract::A flow cytometric assay that measures binding of human T-lymphotropic virus type 1 (HTLV-1) virions to target cells was used to investigate the binding process and to screen for compounds affecting viral binding. Results showed that adenosine receptor type 2 antagonists effectively inhibit viral binding at concentrati...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(03)00048-0
更新日期:2003-05-01 00:00:00
abstract::There are currently no effective drugs to treat serious complications caused by WNV infection. The inhibition of WNV by the pluripotent immunomodulator AS101 [ammonium trichloro(dioxyethylene-0-0')tellurate] was evaluated in vitro and in vivo, and its mechanism was explored. Adding AS101 to Vero cells 1h or 5 min befo...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.03.004
更新日期:2012-06-01 00:00:00
abstract::A temperature-sensitive mutant of the Moloney murine leukemia virus-TB, ts1, causes hindlimb paralysis and immunodeficiency in mice. At the restrictive temperature, the envelope precursor polyprotein, gPr80env, is inefficiently processed intracellularly, and this is associated with the neurovirulence of ts1. To test t...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(91)90096-e
更新日期:1991-03-01 00:00:00
abstract::A transmissible disease of the plant pathogenic fungus Helminthosporium victoriae, the causal agent of Victoria blight of oats, was reported more than 50 years ago. Diseased, but not normal, isolates, of H. victoriae contain two distinct viruses designated according to their sedimentation values as victorivirus Helmin...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2015.12.011
更新日期:2016-02-02 00:00:00
abstract::The nucleotide sequence of the matrix gene (M) of a recently identified morbillivirus, phocid distemper virus (PDV), was determined and the amino acid composition deduced. The M gene of PDV shared many characteristics with the corresponding gene in other morbilliviruses. The nucleotide homology with the closely relate...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(92)90064-g
更新日期:1992-04-01 00:00:00
abstract::Highly pathogenic avian H5N1 influenza A viruses occasionally infect humans and cause severe respiratory disease and fatalities. Currently, these viruses are not efficiently transmitted from person to person, although limited human-to-human transmission may have occurred. Nevertheless, further adaptation of avian H5N1...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2013.07.017
更新日期:2013-12-05 00:00:00
abstract::In recent years, the spread of Duck Hepatitis A Viruses (DHAVs) has represented a serious threat and significant economic impact in duck industry of China. The sixteen reported DHAV isolates (15 DHAV-1 strains and one DHAV-3) were identified from infected ducks with clinical symptoms in China between 2009 and 2012. In...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2013.10.007
更新日期:2013-12-26 00:00:00
abstract::Citrus psorosis (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV) belong to the family Ophioviridae, plant viruses with filamentous nucleocapsids and segmented genomes of negative polarity, causing the worldwide distributed citrus psorosis and lettuce big-vein diseases, respectively. To gain insight into the replic...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.08.005
更新日期:2012-12-01 00:00:00
abstract::Dengue (Den) viruses cause apoptosis in mammalian cells, but usually result in high progeny yields without evident damage in mosquito cells. By using subtractive hybridization, 13 potentially virus-induced genes were selected in Den-2 virus-infected Aedes albopictus C6/36 cells. Based on semi-quantitative and real-tim...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2006.11.002
更新日期:2007-03-01 00:00:00
abstract::Infectious salmon anaemia virus (ISAV) is an orthomyxovirus causing anaemia and circulatory disease with high mortality in farmed Atlantic salmon (Salmo salar). Orthomyxoviruses are unusual as RNA viruses as they replicate in the nucleus and some viral transcripts undergo splicing. The nuclear replication necessitates...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2013.07.001
更新日期:2013-10-01 00:00:00
abstract::Transplacental viral infections are dependent upon complex interactions between feto-placental and maternal immune responses and the stage of fetal development at which the infection occurs. Bovine viral diarrhea virus (BVDV) has the ability to cross the placenta and infect the fetus. Infection early in gestation with...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.05.018
更新日期:2012-08-01 00:00:00
abstract::SARS coronavirus (CoV) papain-like protease (PLpro) reportedly induced the production of TGF-β1 through p38 MAPK/STAT3-meidated Egr-1-dependent activation (Sci. Rep. 6, 25754). This study investigated the correlation of PLpro-induced TGF-β1 with the expression of Type I collagen in human lung epithelial cells and mous...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2017.04.008
更新日期:2017-05-02 00:00:00
abstract::In the early 1970s, the Chinese Equine Infectious Anemia Virus (EIAV) vaccine, EIAV(DLA), was developed through successive passages of a wild-type virulent virus (EIAV(L)) in donkeys in vivo and then in donkey macrophages in vitro. EIAV attenuation and cell tropism adaptation are associated with changes in both envelo...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2007.04.005
更新日期:2007-09-01 00:00:00
abstract::Analysis of complete polyprotein-encoding sequences of the two most prevalent genotypes of hepatitis C virus (HCV-1a and HCV-1b) revealed evidence of abundant, slightly deleterious nonsynonymous variants subject to ongoing purifying selection. In the case of both HCV-1a and HCV-1b, the NS3 protein demonstrated a high ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2009.11.001
更新日期:2010-02-01 00:00:00
abstract::Monocotyledonous plant infecting mastreviruses (family Geminiviridae) have been found in the Old World. The greatest diversity of these viruses has been found in Africa but this may simply reflect the more extensive sampling that has been done there. To provide a better understanding of mastrevirus diversity in Austra...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.07.018
更新日期:2012-10-01 00:00:00
abstract::In adenovirus type 12 (Ad12)-induced tumor cells, in Ad12-transformed cells and in continuously passaged cell lines from these sources, the viral DNA is integrated in multiple copies, usually at a single chromosomal location. In different tumors or cell lines, the sites of integration of Ad12 DNA are all different. Ra...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(98)00131-2
更新日期:1999-01-01 00:00:00
abstract::The nucleotide sequence of the E-3 region genes, the hexon associated protein pVIII genes, and part of the fiber genes coding for the N-terminal tail regions, of porcine adenovirus (PAV) types 1 and 2 were determined. The sizes of the E-3 regions were found to be 1162 and 1222 bp, respectively. The five open reading f...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(96)01336-6
更新日期:1996-08-01 00:00:00
abstract::Emergent agricultural pathogens cause severe damage worldwide and their invasive potential is significantly increased by global trade, crop intensification and climate change. Standard surveillance and diagnostic protocols need to be evaluated and implemented, particularly with diseases caused by a wide range of patho...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2020.197959
更新日期:2020-08-01 00:00:00
abstract::Crimean-Congo haemorrhagic fever virus (CCHFv) is a member of the genus Nairovirus in the family Bunyaviridae. It possesses a tripartite, single stranded RNA genome of negative polarity consisting of large (L), medium (M) and small (S) segments. CCHF virus is enzootic in life stock and wild animals in many parts of th...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2003.12.035
更新日期:2004-06-15 00:00:00
abstract::To identify the parts of retroviral integrase that interact with its DNA substrates, we compared the patterns of target site usage by chimeric enzymes and protein fragments in assays that reveal integrase's non-specific nuclease activities. The central region of 12 chimeric proteins between the human immunodeficiency ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(99)00126-4
更新日期:2000-01-01 00:00:00
abstract::The mild climate of the Salinas Valley, CA lends itself well to a diverse agricultural industry. However, the diversity of weeds, crops and insect and fungal vectors also provide favorable conditions for plant virus disease development. This paper considers the incidence and management of several plant viruses that ha...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/s0168-1702(00)00196-9
更新日期:2000-11-01 00:00:00
abstract::Double-stranded RNA viruses infecting bacterial hosts belong to the Cystoviridae family. Bacteriophage phi6 is one of the best characterized dsRNA viruses and shares structural as well as functional similarities with other well-studied eukaryotic dsRNA viruses (e.g. L-A, rotavirus, bluetongue virus, and reovirus). The...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2003.12.009
更新日期:2004-04-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV), an arterivirus, belongs to a group of RNA viruses that are cytopathic for macrophages and establish persistent infections. Apoptosis is the presumed mechanism of cell death in monkey kidney cell lines and porcine alveolar macrophages after infection with Eur...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(02)00029-1
更新日期:2002-05-10 00:00:00