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Development of a stable insect cell line constitutively expressing rotavirus VP2.

Abstract：

:An insect High-Five cell line was generated constitutively and stably expressing the VP2 protein of rotavirus RF strain leading to the formation of core-like particles. The integration of the VP2 gene was confirmed by PCR, Real-time PCR and Southern blot, and the protein expression was confirmed by Western blot and immunofluorescence microscopy. Integrity and self assembly of VP2 as core-like particles was demonstrated by electron microscopy. The High-Five cell system stably expressing rotavirus core-like particles can be applied to the study of viral protein structure and functions; it may be useful for vaccine development, gene therapy and drug delivery.

journal_name

Virus Res

journal_title

Virus research

authors

Shoja Z,Tagliamonte M,Jalilvand S,Mokhtari-Azad T,Hamkar R,Shahmahmoodi S,Rezaei F,Tornesello M,Buonaguro FM,Buonaguro L,Nategh R

doi

10.1016/j.virusres.2012.12.015

subject

Has Abstract

pub_date

2013-03-01 00:00:00

pages

66-74

issue

1-2

eissn

0168-1702

issn

1872-7492

pii

S0168-1702(12)00477-7

journal_volume

172

pub_type

杂志文章

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