Abstract：
:African horse sickness (AHS) is a devastating viral disease affecting equines and has resulted in many disastrous epizootics. To date, no successful therapeutic treatment exists for AHS, and commercially used live-attenuated vaccines have various undesirable side effects. Previous studies have shown that mice inoculated with insoluble African horse sickness virus (AHSV) VP7 crystals are protected from live challenge with a lethal dose of AHSV. This study investigates the humoral and cell-mediated immune responses in guinea-pigs to a safer monovalent vaccine alternative based on AHSV-5 VP7 quasi-crystals produced in plants. Guinea-pigs received prime- and boost-inoculations of between 10 and 50 μg of purified plant-produced AHSV VP7. Western immunoblot analysis of the humoral response showed stimulation of high titres of anti-VP7 antibodies 28 days after the boost-inoculation in sera from three of the five experimental animals. In addition, RNA-seq transcriptome profiling of guinea-pig spleen-derived RNA highlighted thirty significantly (q ≤ 0.05) differentially expressed genes involved in innate and adaptive immunity. Differential expression of genes involved in Th1, Th2 and Th17 cell differentiation suggest a cell-mediated immune response to AHSV-5 VP7. Upregulation of several important cytokines and cytokine receptors were noted, including TNFSF14, CX3CR1, IFNLR1 and IL17RA. Upregulation of IL17RA suggests a Th17 response which has been reported as a key component in AHSV immunity. While further investigation is needed to validate these findings, these results suggest that AHSV-5 VP7 quasi-crystals produced in N. benthamiana are immunogenic and induce both humoral and cell-mediated responses.
journal_name
Virus Resjournal_title
Virus researchauthors
Fearon SH,Dennis SJ,Hitzeroth II,Rybicki EP,Meyers AEdoi
10.1016/j.virusres.2020.198284subject
Has Abstractpub_date
2021-01-06 00:00:00pages
198284eissn
0168-1702issn
1872-7492pii
S0168-1702(20)31191-6journal_volume
294pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract：:Bovine herpesvirus-1 infected cell protein 27 (BICP27) was detected predominantly in the nucleolus. The open reading frame of BICP27 was fused with the enhanced yellow fluorescent protein (EYFP) gene to investigate its subcellular localization in live cells and BICP27 was able to direct monomeric, dimeric or trimeric ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.07.024
更新日期：2009-11-01 00:00:00
abstract：:Cucurbit chlorotic yellows virus (CCYV) is a new member of the genus Crinivirus (family Closteroviridae) with a bi-partite genome. CCYV RNA 1-encoded p22 has recently been reported to be a weak local suppressor of RNA silencing for which an interaction with cucumber SKP1LB1 through an F-box-like motif was demonstrated...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2020.197887
更新日期：2020-04-02 00:00:00
abstract：:Despite the fact that cells infected with wild type vaccinia virus synthesize viral DNA and assemble progeny virus particles within the cytoplasm, the host cell nucleus is required for a productive infection. Recent evidence suggests that vaccinia virus selectively recruits components from the host cell nucleus into t...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/0168-1702(87)90014-1
更新日期：1987-09-01 00:00:00
abstract：:Baculoviruses are a promising gene delivery vector. They have the ability to express large transgenes in mammalian cells without displaying pathogenicity in humans; however, little is known about their transduction mechanisms in target cells. In this study, we use colocalization and live-cell imaging studies to elucid...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.01.006
更新日期：2014-03-06 00:00:00
abstract：:In this study, we tried to identify dengue virus-specific CD4(+) T-cell epitopes, which can induce PBMC (peripheral blood mononuclear cells) isolated from DF convalescent patients (dengue virus type 1 infection) to secrete IFN-gamma. PBMC of DF convalescent patients were stimulated in vitro with dengue virus-derived p...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2007.10.010
更新日期：2008-03-01 00:00:00
abstract：:Recent scientific advances are providing an opportunity to revisit strategies for cervical cancer prevention. How to invest health resources wisely, such that clinical benefits are maximized-and opportunity costs are minimized-is a critical question in the setting of new technology. In addition to an intervention's ef...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/s0168-1702(02)00199-5
更新日期：2002-11-01 00:00:00
abstract：:Porcine epidemic diarrhea virus (PEDV) has plagued the domestic swine industry in Korea causing significant economic impacts on pig production nationwide. In the present study, we determined the complete nucleotide sequences of the spike (S) glycoprotein genes of seven Korean PEDV isolates. The entire S genes of all i...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2010.01.015
更新日期：2010-05-01 00:00:00
abstract：:Primers corresponding to conserved regions in the RNA-dependent RNA polymerase and the RACE procedure led to the cloning of the complete sweetpotato mild mottle virus (SPMMV) RNA genome. The assembled SPMMV genomic sequence was 10,818 nucleotides in length with a polyadenylated tract at the 3' terminus. The structure ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(97)00148-2
更新日期：1998-02-01 00:00:00
abstract：:Cricket paralysis virus (Dicistroviridae: Cripavirus) (CrPV) naturally has a wide range of insect hosts which is reflected in its ability to infect several cultured insect cell lines. The expression of viral gene products is controlled by two kinds of internal ribosome entry site (IRES) elements, 5' and intergenic (IG...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(03)00139-4
更新日期：2003-08-01 00:00:00
abstract：:The sub-cellular location and mechanism of membrane association of NS3 and NS2-3 polypeptides of bovine viral diarrhoea virus (BVDV) have been examined. Both NS3 and NS2-3 proteins were detected in post-nuclear membrane fractions but not in cytosolic fractions of BVDV infected cells; a proportion of NS3, but not NS2-3...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(03)00223-5
更新日期：2003-11-01 00:00:00
abstract：:Raccoons (Procyon lotor), which are not native to Japan, have been suspected to transmit various pathogens by frequent intrusion into agricultural and residential areas. To determine influenza A virus seropositivity in raccoons in Japan, we examined a total of 634 raccoons captured in 19 towns (A-S) from 2009 to 2012....
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.05.004
更新日期：2014-08-30 00:00:00
abstract：:Vesicular stomatitis virus (VSV) is a prototypic nonsegmented negative-strand RNA virus. VSV's broad cell tropism makes it a popular model virus for many basic research applications. In addition, a lack of preexisting human immunity against VSV, inherent oncotropism and other features make VSV a widely used platform f...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2013.06.003
更新日期：2013-09-01 00:00:00
abstract：:White spot syndrome virus immediate early (IE) gene wsv249 encodes an E3 ubiquitin ligase that can interact with a shrimp ubiquitin-conjugating enzyme to mediate ubiquitination. In this study, to understand the transcriptional regulation of wsv249, a serial of 5'-truncated mutations were made on its promoter and the a...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2018.05.012
更新日期：2018-07-02 00:00:00
abstract：:The protein and glycoprotein composition of a sucrose gradient fraction from vaccinia infected cells treated with rifampicin was studied. This particulate fraction contained cytoplasmic membranes and pleomorphic membranous structures. The glycoproteins (89, 42 and 20-23 kDa, respectively) were identified as the same g...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(90)90077-o
更新日期：1990-08-01 00:00:00
abstract：:Tissue culture adaptation of infectious bursal disease virus (IBDV) results in alternation of three residues on its major capsid protein VP2 and these residues may engage in receptor binding. Although the key of successful infection of tissue culture adapted IBDV in tissue cultures was defined as the virus entering st...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2006.08.001
更新日期：2007-01-01 00:00:00
abstract：:Nucleic acid probes prepared directly from bluetongue virus (BTV) genomic double-stranded RNA (dsRNA) have been used to identify the functionally equivalent genome segments from six distinct isolates of BTV after their separation in both agarose and polyacrylamide gel electrophoresis systems. Variations in the rate, a...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(88)90078-0
更新日期：1988-06-01 00:00:00
abstract：:Tobacco (cv. Xanthi nn) plants were watered with allopurinol [4-hydroxypyrazolo (3,4-d) pyrimidine, HPP], a xanthine oxidoreductase (XOR) inhibitor, to investigate its effects on infection by Tobacco mosaic virus engineered to express the green fluorescent protein (TMV.GFP). TMV.GFP infection was monitored by examinat...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.05.018
更新日期：2008-11-01 00:00:00
abstract：:There are currently no effective drugs to treat serious complications caused by WNV infection. The inhibition of WNV by the pluripotent immunomodulator AS101 [ammonium trichloro(dioxyethylene-0-0')tellurate] was evaluated in vitro and in vivo, and its mechanism was explored. Adding AS101 to Vero cells 1h or 5 min befo...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.03.004
更新日期：2012-06-01 00:00:00
abstract：:Epstein-Barr virus (EBV), a human herpesvirus, is linked to both epithelial and lymphoid malignancies. Induction of EBV reactivation is a potential therapeutic strategy for EBV-associated tumors. In this study, we assessed the effects of rapamycin on EBV reactivation in gastric carcinoma cells. We found that rapamycin...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2017.11.021
更新日期：2018-01-15 00:00:00
abstract：:The interaction between UV-B irradiation and cutaneous human papillomaviruses (HPV) has been suggested to be of relevance for the development of non-melanoma skin cancers. We investigated the activity within the upstream regulatory region (URR) of the HPV types 8, 38, 92, 93 and 96, as well as their responsiveness to ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.07.013
更新日期：2008-11-01 00:00:00
abstract：:The uptake of porcine parvovirus (PPV) into host cells, permissive or nonpermissive for PPV replication, was monitored by autoradiography, immunofluorescent microscopy, and dual parameter flow cytometry. While both permissive and nonpermissive cells selectively took up the light (activated) form of the virus from a mi...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(88)90054-8
更新日期：1988-04-01 00:00:00
abstract：:We performed phylogenetic and cluster analysis of human rhinovirus species A (HRV-A) isolated from 76 children with acute respiratory infection in Yamagata prefecture, Japan during the period 2003-2007. Phylogenetic trees based on the nucleotide and amino acid sequences of the VP4/VP2 coding region showed that the pre...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.11.011
更新日期：2010-02-01 00:00:00
abstract：:Adenoviruses, types 2 and 12 induce amplification of SV40 DNA sequences in cells of the SV40-transformed human newborn kidney cell line, NB-E. Similarly, integrated hepatitis B virus DNA sequences in the human hepatoma cell line, PLC/*PRF/5, and bovine papillomavirus (BPV) DNA sequences in BPV-transformed mouse cells ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(90)90079-q
更新日期：1990-08-01 00:00:00
abstract：:Bovine enteroviruses, which belong to the Picornaviridae family, can cause clinical symptoms in cattle and are excreted in feces. In this study, a cytolytic virus was isolated from Madin-Darby bovine kidney (MDBK) cells from fecal samples of bovine with severe diarrhea and hemorrhagic intestinal mucosa that had been o...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.07.032
更新日期：2014-10-13 00:00:00
abstract：:Utilizing the reverse transcriptase-polymerase chain reaction (RT-PCR) procedure, we have synthesized full-length copies of segment 10 from African horsesickness virus (AHSV) serotypes 1, 4 and 8. The genes were cloned, sequenced and compared with the sequence of the cognate gene from AHSV serotypes 3 and 9. Sequences...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(94)90052-3
更新日期：1994-08-01 00:00:00
abstract：:Bats are reservoir hosts of many zoonotic viruses and identification of viruses that they carry is important. This study aimed to use high throughput screening to identify the viruses in fecal guano of Taiwanese insectivorous bats caves in order to obtain more information on bat-derived pathogenic viruses in East Asia...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2018.10.020
更新日期：2019-01-02 00:00:00
abstract：:Satellite RNAs (satRNAs) and viroids belong to the group called subviral agents and are the smallest pathogens of plants. In general, small satRNAs and viroids are 300-400 nt in size and do not encode any functional proteins; they are thus regarded as so-called long noncoding RNAs (lncRNAs). These lncRNAs are receivin...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2015.06.016
更新日期：2016-01-02 00:00:00
abstract：:Hepatitis C virus (HCV) is a major cause of global morbidity and mortality, with an estimated 130-150 million infected individuals worldwide. HCV is a leading cause of chronic liver diseases including cirrhosis and hepatocellular carcinoma. Current treatment options in developing countries involve pegylated interferon...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.07.008
更新日期：2016-09-02 00:00:00
abstract：:The mammalian reoviruses possess a genome composed of 10 double stranded RNA segments. The serotype 1 strain Lang M1 segment was sequenced and compared to the published type 3 sequence. Both segments were 2304 base-pairs long coding for the mu 2 protein predicted to be 736 amino acids long. The sequences were highly c...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(92)90042-8
更新日期：1992-02-01 00:00:00
abstract：:Single-cell sequencing (SCS) has emerged as a valuable tool to study cellular heterogeneity in diverse fields, including virology. By studying the viral and cellular genome and/or transcriptome, the dynamics of viral infection can be investigated at single cell level. Most studies have explored the impact of cell-to-c...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2016.10.016
更新日期：2017-07-15 00:00:00
