Abstract:
:The sub-cellular location and mechanism of membrane association of NS3 and NS2-3 polypeptides of bovine viral diarrhoea virus (BVDV) have been examined. Both NS3 and NS2-3 proteins were detected in post-nuclear membrane fractions but not in cytosolic fractions of BVDV infected cells; a proportion of NS3, but not NS2-3, could be dissociated from the membranes with 800 mM KCl or at pH 11. Following extraction with 1% Triton X-114, NS3 was predominantly present in the aqueous phase, but NS2-3 was only recovered in the detergent phase. Confocal microscopy showed that in BVDV infected cells, NS3 and/or NS2-3 co-localise with the endoplasmic reticulum (ER) protein, ERP60, but not Golgi or lysosomal proteins. Sub-cellular fractionation analysis demonstrated that NS2-3 was almost exclusively associated with the rough ER membrane but a significant proportion of NS3 was present in the smooth ER membrane fractions in addition to the rough ER membrane. These differences in the distribution of NS2-3 and NS3 on ER membranes in cells infected with cytopathogenic (CP) strains of BVDV were also observed using confocal microscopy and antibodies that are specific to either NS2 or NS3. This distinct distribution of NS3 and NS2-3 on the ER membrane has revealed a further difference between CP and non-cytopathogenic (NCP) strains of BVDV.
journal_name
Virus Resjournal_title
Virus researchauthors
Zhang G,Flick-Smith H,McCauley JWdoi
10.1016/s0168-1702(03)00223-5subject
Has Abstractpub_date
2003-11-01 00:00:00pages
89-102issue
2eissn
0168-1702issn
1872-7492pii
S0168170203002235journal_volume
97pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract::The complete nucleotide sequence has been determined for the S RNA of Aino virus, a member of the Simbu serogroup (Bunyavirus genus, family Bunyaviridae). The S RNA is 850 nucleotides long (2.76 X 10(5) daltons) and in the viral complementary sequence has a short 5' non-coding region of 34 nucleotides and a more exten...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(84)90034-0
更新日期:1984-01-01 00:00:00
abstract::The DNA polymerase from Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) was identified and sequenced, and its amino acid sequence was compared with other viral DNA polymerases to identify conserved regions and to reconstruct a phylogenetic tree. The sequence analysis of the AgMNPV DNA polymerase gene revealed the ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2005.01.010
更新日期:2005-06-01 00:00:00
abstract::The genomes of eight hepatitis A virus (HAV) strains originating from far distant geographic regions such as Europe, North Africa, Middle and North America, Australia and The People's Republic of China were compared by RNase T1 oligonucleotide mapping. For this purpose, the viruses were propagated in cell cultures and...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(85)90020-6
更新日期:1985-12-01 00:00:00
abstract::The A antigen gene from a very virulent strain of Marek's disease virus, RB-1B, has been cloned and the nucleotide sequence determined. The predicted amino acid sequence showed 99% identity to that determined for the MDV GA A antigen (Coussens and Velicer, J. Virol. 62, 2373-2379, 1988) over all but the carboxy-termin...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(89)90094-4
更新日期:1989-04-01 00:00:00
abstract::A simple and sensitive method for the detection of measles virus genome was developed, amplifying the regions encoding the nucleocapsid (N) protein and hemagglutinin (H) protein of measles virus by reverse transcriptase-polymerase chain reaction (RT-PCR). We examined a variety of measles patients: 28 patients with nat...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(94)00074-m
更新日期:1995-01-01 00:00:00
abstract::With the aim to detect what kind of cells, in addition to erythroid progenitors, could be involved in the pathogenesis of B19 infection in some connective tissue diseases, primary cultures of human fibroblasts (HF) and endothelial cells (HUVEC) were exposed to a B19 positive serum (350 genome copies/cell). The presenc...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2005.05.003
更新日期:2005-12-01 00:00:00
abstract::Noroviruses belong to the Caliciviridae family of single-stranded positive-sense RNA viruses. The genus Norovirus includes seven genogroups (designated GI-GVII), of which GI, GII and GIV infect humans. Human noroviruses are responsible for widespread outbreaks of acute gastroenteritis and represent one of the most com...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2016.12.018
更新日期:2017-04-15 00:00:00
abstract::Positive-strand RNA viruses exist as a quasi-species due to the incorporation of mutations into the viral genome during replication by the virus-encoded RNA-dependent RNA polymerase (RdRP). Therefore, the RdRP is often described as a low-fidelity enzyme. However, until recently, a complete description of the kinetic, ...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2004.11.004
更新日期:2005-02-01 00:00:00
abstract::In this study we showed that the virulent Moscow strain of Ectromelia virus (ECTV-MOS) infection leads to induction of apoptosis in the BALB/c mouse central nervous system. ECTV-MOS-infected cells and inflammation sites were found in brain parenchyma between 5 and 15 days after footpad infection with ECTV-MOS. Infecte...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2005.08.001
更新日期:2006-02-01 00:00:00
abstract::Recently, a novel deoxyribonucleic acid (DNA) virus, designated SEN virus (SENV), was discovered and strong associations between the two SENV variants (SENV-D and SENV-H) and non-A to E hepatitis were reported. To clarify the character of SENV infection in children, we investigated the detection rates of serum SENV DN...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2003.12.028
更新日期:2004-03-15 00:00:00
abstract::We have previously identified an inhibitory, 57 nt AU-rich sequence in the HPV-1 late 3' UTR, termed as the HPV-1 AU-rich element (h1ARE). It contains two types of functionally important motifs: two AUUUA sequences and three UUUUU sequences. We have shown that the h1ARE interacts with heterogeneous nuclear ribonucleop...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(00)00238-0
更新日期:2001-03-01 00:00:00
abstract::Prior studies on PRRSV strain VR-2332 non-structural protein 2 (nsp2) had shown that as much as 403 amino acids could be removed from the hypervariable region without losing virus viability in vitro. We utilized selected nsp2 deletion mutants to examine in vivo growth. Young swine (4 pigs/group; 5 control swine) were ...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2010.07.024
更新日期:2010-12-01 00:00:00
abstract::Subtype C is the most prevalent HIV-1 subtype globally and a common circulating recombinant form virus, CRF_BC, is predominant in China. It is unclear whether subtype C-based vaccines are capable of neutralizing CRF_BC viruses or can be evaluated using a neutralization assay based on CRF_BC pseudoviruses. Phylogenetic...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2010.09.012
更新日期:2011-01-01 00:00:00
abstract::Variants assigned to GI-23 lineage of infectious bronchitis virus (IBV), formerly called Var2, have circulated for nearly 20 years only in countries of the Middle East. Strains of this lineage were first identified in Israel in 1998. More severe form of the virus appeared in 2006, when the second wave of Var2 epidemic...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2017.09.010
更新日期:2017-10-15 00:00:00
abstract::Translation initiation of the coxsackievirus B3 (CVB3) RNA occurs by internal ribosomal entry. The internal ribosomal entry site (IRES) of the virus has been mapped to the 5' untranslated region (5' UTR) of the genome. As well, the 5' UTR has been suggested to play roles in determining the tissue tropism and infectivi...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(02)00138-7
更新日期:2002-12-01 00:00:00
abstract::A disease of Rudbeckia hirta (Black-eyed Susan), characterized by severe flower deformation, was observed in Minnesota during 2010-2016. A previously undescribed virus species, named Rudbeckia flower distortion virus (RuFDV, family Caulimoviridae, genus unassigned), was determined to be the causal agent of the disease...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2017.05.012
更新日期:2017-09-15 00:00:00
abstract::Hamster polyomavirus (HaPyV) major capsid protein VP1 based chimeric virus-like particles (VLPs) carrying model GP33 CTL epitope derived from Lymphocytic choriomeningitis virus (LCMV) were generated in yeast and examined for their capability to induce CTL response in mice. Chimeric VP1-GP33 VLPs were effectively proce...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2011.08.003
更新日期:2012-01-01 00:00:00
abstract::A wide use of inhibitors of influenza virus neuraminidase (NAIs) to control influenza in humans demands a better understanding of the mechanisms involved in the resistance emergence. In vitro studies demonstrate that both neuraminidase (NA) and hemagglutinin (HA) influence virus susceptibility to NAIs. Drug resistance...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2004.02.034
更新日期:2004-07-01 00:00:00
abstract::The complete sequence was obtained for two variants of raspberry vein chlorosis virus (RVCV), confirming that this virus is a rhabdovirus most closely related to the cytorhabdoviruses alfalfa dwarf virus and strawberry crinkle virus. The two RVCV variants share only a 68% nucleotide sequence identity so that the previ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2019.03.004
更新日期:2019-05-01 00:00:00
abstract::The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac78 gene is one of the baculovirus core genes. Recent studies showed that ac78 is essential for budded virion (BV) production and the embedding of occlusion-derived virion (ODV) into occlusion body during the AcMNPV life cycle. Here, we report that an ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2014.07.019
更新日期:2014-10-13 00:00:00
abstract::Human recombinant alpha interferon, A/D, significantly reduced the replication and cell fusion induced by herpes simplex virus type 1 in monkey cells. Thin-section electron microscopy of interferon-treated monkey cells showed distinct assembly of nucleocapsids within the nucleus. Analysis of virus-specific proteins by...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(89)90051-8
更新日期:1989-01-01 00:00:00
abstract::The human respiratory coronavirus OC43 was grown on a human rectal tumor cell line and was isotopically labeled with amino acids, glucosamine, and orthophosphate to analyze virion structural proteins. Four major protein species were resolved by electrophoresis and many of their properties were deduced from digestion s...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(86)90013-4
更新日期:1986-08-01 00:00:00
abstract::From the sera of patients with advanced cancer, a novel factor called SDF (serum-derived factor) was partially purified. SDF was shown to stimulate transcription from the long terminal repeat (LTR) of human immunodeficiency virus type 1 (HIV-1) by transient CAT assay. It did not stimulate gene expression of various co...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(93)90065-u
更新日期:1993-09-01 00:00:00
abstract::Viruses frequently spread among cells or hosts in groups, with multiple viral genomes inside the same infectious unit. These collective infectious units can consist of multiple viral genomes inside the same virion, or multiple virions inside a larger structure such as a vesicle. Collective infectious units deliver mul...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2019.03.013
更新日期:2019-05-01 00:00:00
abstract::The beta genus comprises more than 50 beta human papillomavirus (HPV) types that are suspected to be involved, together with ultraviolet (UV) irradiation, in the development of non-melanoma skin cancer (NMSC), the most common form of human cancer. Two members of the genus beta, HPV5 and HPV8, were first identified in ...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2016.11.013
更新日期:2017-03-02 00:00:00
abstract::In alfalfa samples analyzed by hightroughput sequencing, four de novo assembled contigs encoding gene products showing identities to alphapartitiviruses proteins were found based on BlastX analysis. The predicted amino acid (aa) sequences of two contigs presented 99-100% identity to the RNA-dependent RNA polymerase (R...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2019.03.021
更新日期:2019-05-01 00:00:00
abstract::The aim of the present study was to analyze the influence of virus origin, mammalian or mosquito cell-derived, on antiviral susceptibility of DENV-2 to entry inhibitors and the association of this effect with any alteration in the mode of entry into the cell. To this end, ten serial passages of DENV-2 were performed i...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2014.02.011
更新日期:2014-05-12 00:00:00
abstract::Nineteen distinct measles virus (MV) strains associated with nine different genotypes were identified in five Australian states (Victoria, New South Wales, Queensland, Northern Territory and Western Australia) between 1999 and 2001. One of the strains identified is likely to represent a new genotype within the clade D...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(02)00273-3
更新日期:2003-02-01 00:00:00
abstract::Despite the fact that cells infected with wild type vaccinia virus synthesize viral DNA and assemble progeny virus particles within the cytoplasm, the host cell nucleus is required for a productive infection. Recent evidence suggests that vaccinia virus selectively recruits components from the host cell nucleus into t...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/0168-1702(87)90014-1
更新日期:1987-09-01 00:00:00
abstract::Highly pathogenic H5N1 influenza viruses have been isolated from a number of avian and mammalian species. Despite intensive control measures the number of human and animal cases continues to increase. A more complete understanding of susceptible species and of contributing environmental and molecular factors is crucia...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2013.09.004
更新日期:2013-12-05 00:00:00