Abstract：
:Baculoviruses are a promising gene delivery vector. They have the ability to express large transgenes in mammalian cells without displaying pathogenicity in humans; however, little is known about their transduction mechanisms in target cells. In this study, we use colocalization and live-cell imaging studies to elucidate the internalization and intracellular trafficking pathways of baculoviruses through direct visualization of VP39-GFP-labeled viral particles and various endocytic structures within target cells. Drug inhibition and confocal microscopy results suggested that baculoviruses enter the cells via clathrin-mediated endocytosis in a dynamin-dependent manner. Viral particles were shown to traffic through early endosomes, triggering a low-pH-dependent endosomal fusion process of viruses. Suppressed autophagy activity enhanced viral transduction and overexpression of autophagosomes reduced viral transduction, suggesting that autophagy is involved in degradation process of viral particles. Actin filaments were involved in the viral transduction, while microtubules negatively regulated viral transduction by facilitating the fusion of autophagosomes with lysosomes to form autolysosomes, where degradation of viral particles occurs. These results shed some light on the essential cellular factors limiting viral transduction, which can be used to improve the use of baculoviral vectors in cell and gene therapy.
journal_name
Virus Resjournal_title
Virus researchauthors
Liu Y,Joo KI,Lei Y,Wang Pdoi
10.1016/j.virusres.2014.01.006subject
Has Abstractpub_date
2014-03-06 00:00:00pages
81-91eissn
0168-1702issn
1872-7492pii
S0168-1702(14)00008-2journal_volume
181pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract：:We have studied which steps are enhanced in the infectious cycle of influenza A virus in Madin-Darby canine kidney (MDCK) cells, a cell line investigated for use in the production of an influenza vaccine because of its ability to yield high levels of virus. We have confirmed that MDCK had the highest production levels...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.04.028
更新日期：2008-09-01 00:00:00
abstract：:The A antigen gene from a very virulent strain of Marek's disease virus, RB-1B, has been cloned and the nucleotide sequence determined. The predicted amino acid sequence showed 99% identity to that determined for the MDV GA A antigen (Coussens and Velicer, J. Virol. 62, 2373-2379, 1988) over all but the carboxy-termin...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90094-4
更新日期：1989-04-01 00:00:00
abstract：:Double-stranded RNA viruses infecting bacterial hosts belong to the Cystoviridae family. Bacteriophage phi6 is one of the best characterized dsRNA viruses and shares structural as well as functional similarities with other well-studied eukaryotic dsRNA viruses (e.g. L-A, rotavirus, bluetongue virus, and reovirus). The...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2003.12.009
更新日期：2004-04-01 00:00:00
abstract：:Persisting DNA of parvovirus H-1 could be demonstrated in cells of two human lymphoma cell lines, the Burkitt lymphoma cell line BL2 and the T-cell leukemia cell line Jurkat which survived infection with parvovirus H-1. Persistence of H-1 DNA rendered the cells resistant to a second H-1 infection. This resistance to H...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(90)90024-6
更新日期：1990-06-01 00:00:00
abstract：:Porcine reproductive and respiratory syndrome (PRRS) is a major disease affecting pigs worldwide and resulting in considerable economic losses. While PRRS is a global phenomenon, the causative viruses PRRSV-1 (first detected in Europe) and PRRSV-2 (isolated in North America) are genetically and biologically distinct. ...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2014.12.026
更新日期：2015-04-16 00:00:00
abstract：:Infectious salmon anaemia virus (ISAV) is a piscine orthomyxovirus causing a serious disease in farmed Atlantic salmon (Salmo salar L.). The virus surface glycoprotein hemagglutinin-esterase (HE) is responsible for both viral attachment and release. Similarity to bovine and porcine torovirus hemagglutinin-esterase (BT...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2010.03.020
更新日期：2010-08-01 00:00:00
abstract：:RNA species of plant tripartite RNA viruses show distinct translational activities in vitro when the viral RNA concentration is high. However, it is not known what causes the differential translation of virion RNAs. Using an in vitro wheat germ translation system, we investigated the translation efficiencies and compe...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(00)00120-9
更新日期：2000-02-01 00:00:00
abstract：:African horse sickness (AHS) is a devastating viral disease affecting equines and has resulted in many disastrous epizootics. To date, no successful therapeutic treatment exists for AHS, and commercially used live-attenuated vaccines have various undesirable side effects. Previous studies have shown that mice inoculat...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2020.198284
更新日期：2021-01-06 00:00:00
abstract：:The pathogenesis of chorioallantoic membrane (CAM) infection with herpes simplex virus 1 and 2 (HSV-1 and HSV-2) as well as chick embryo fibroblast (CEF) passaged HSV-1 was studied. It was found that HSV-2 is at least a million-fold more virulent than HSV-1 as measured by pfu/LD50 ratios for the embryo. Serial passage...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(86)90017-1
更新日期：1986-08-01 00:00:00
abstract：:The immune response to a naked plasmid DNA encoding the nucleoprotein (N protein) of porcine transmissible gastroenteritis virus (TGEV) was investigated in this study. A complementary DNA of the entire N gene was amplified by RT-PCR, and inserted into a mammalian expression vector (pcDNA3.1) to construct a recombinant...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(01)00333-1
更新日期：2001-11-28 00:00:00
abstract：:Avian orthoreoviruses cause various diseases in wild birds and domesticated poultry. In this study we report the detection and genomic characterization of a partridge (Perdix perdix) origin reovirus strain, D1007/2008. The virus was isolated on cell culture from acute pneumonia and infra-orbital sinusitis. The 23,497 ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2015.11.018
更新日期：2016-04-02 00:00:00
abstract：:In situ hybridization and virus titration were used to characterize early stages of rat virus (RV) infection of rat pups after oronasal inoculation. Results suggest that virus enters through the lung and that early viremia leads rapidly to pantropic infection. Cells derived from all three germ layers were infected wit...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(93)90085-2
更新日期：1993-04-01 00:00:00
abstract：BACKGROUND:HTLV-2 infections are almost always asymptomatic, and diseases associated with the infection are rarely reported. Little information is available on the relationship between HTLV-2 proviral load and gender or expression of disease, especially among patients with HIV-1 co-infection. METHODS:We studied 77 HTL...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.01.015
更新日期：2008-07-01 00:00:00
abstract：:This review focuses on new or improved technologies currently being applied, or likely to be applied in the future, to worldwide research on plant virus epidemiology. Recent technological advances and innovations provide many opportunities to improve understanding of the way diverse types of plant virus epidemics deve...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2013.11.003
更新日期：2014-06-24 00:00:00
abstract：:The intercellular adhesion molecule-1 (ICAM-1) receptor was expressed in primary chicken embryo cells using a retroviral vector and shown to specifically bind major group human rhinoviruses (HRVs). A truncated, membrane-bound ICAM-1 protein containing N-terminal domains 1, 2, and 3 retained the ability to bind virus w...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(92)90005-t
更新日期：1992-07-01 00:00:00
abstract：:An Olive latent virus 1 isolate designated GM6, obtained from a Portuguese olive tree, was characterized and the coat protein gene sequenced and analysed. The purified virus particles showed to be isometric with ca. 30 nm in diameter and contained a single-stranded RNA species with ca. 3.7 kb. The dsRNA profile obtain...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.09.007
更新日期：2005-03-01 00:00:00
abstract：:Damage to the Kupffer and endothelial cells of the liver sinusoids induced by the administration of sublethal doses of frog virus 3 (FV 3) renders A/J mice which are genetically resistant to mouse hepatitis virus type 3 (MHV 3) highly susceptible to this virus. Liver histopathology of these animals revealed typical ne...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(84)90013-3
更新日期：1984-10-01 00:00:00
abstract：:Fowlpox virus (FWPV) is currently used as a vector to express foreign genes of various poultry and mammalian pathogens. However, due to limited information available about the primary structure of FWPV promoters required for an optimal transcriptional efficiency, the full potential of FWPV as an expression vector has ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2005.08.017
更新日期：2006-03-01 00:00:00
abstract：:Infected cell protein 10 (ICP10) or antigen 4 (Ag4) and infected cell-specific protein 11/12 (ICSP11/12) have been suggested as specific antigenic markers for cervical carcinoma. Experiments were designed to determine whether ICP10 and ICSP11/12 are distinct antigens and to determine the cellular localization of ICP10...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(85)90012-7
更新日期：1985-04-01 00:00:00
abstract：:The purpose of this study was to generate complete genome sequences of Aravan (ARAV), Khujand (KHUV), Irkut (IRKV) and West Caucasian bat (WCBV) viruses, and to compare them with genomes of other lyssaviruses. We focused on RNA-dependent RNA-polymerase (L) and non-coding regions, because other genes of these viruses h...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.04.021
更新日期：2008-09-01 00:00:00
abstract：:Crimean-Congo haemorrhagic fever virus (CCHFv) is a member of the genus Nairovirus in the family Bunyaviridae. It possesses a tripartite, single stranded RNA genome of negative polarity consisting of large (L), medium (M) and small (S) segments. CCHF virus is enzootic in life stock and wild animals in many parts of th...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2003.12.035
更新日期：2004-06-15 00:00:00
abstract：:Felid herpesvirus 1 (FHV-1) mutants were constructed using two-step Red-mediated recombination techniques based on a virulent full-length FHV-1 BAC clone. The individual mutant viruses generated were deficient in glycoprotein C (gC), glycoprotein E (gE), US3 serine/threonine protein kinase (PK), or both gE and thymidi...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.05.001
更新日期：2016-08-02 00:00:00
abstract：:The uptake of porcine parvovirus (PPV) into host cells, permissive or nonpermissive for PPV replication, was monitored by autoradiography, immunofluorescent microscopy, and dual parameter flow cytometry. While both permissive and nonpermissive cells selectively took up the light (activated) form of the virus from a mi...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(88)90054-8
更新日期：1988-04-01 00:00:00
abstract：:A double-stranded (ds) RNA, approximately 9.5kb in size; was identified in the MVC86 isolate of Valsa ceratosperma. Complete sequence of the dsRNA revealed a 9543-bp segment (excluding the 3' poly-A tail) that is predicted to encode a single large protein (P330). P330 has 63%, 49%, and 55% amino acid sequence identiti...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.02.008
更新日期：2012-05-01 00:00:00
abstract：:Marek's disease virus (MDV) is a lymphotropic alphaherpesvirus and causes Marek's disease (MD) in chickens. RLORF4 is an MDV-specific gene located in the repeat long (RL) regions of the genome and is directly involved in attenuation. In this report, we generated recombinant (r)MDVs in which eGFP or mRFP was inserted i...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2015.02.012
更新日期：2015-04-02 00:00:00
abstract：:Human recombinant alpha interferon, A/D, significantly reduced the replication and cell fusion induced by herpes simplex virus type 1 in monkey cells. Thin-section electron microscopy of interferon-treated monkey cells showed distinct assembly of nucleocapsids within the nucleus. Analysis of virus-specific proteins by...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90051-8
更新日期：1989-01-01 00:00:00
abstract：:The complete cDNA sequence corresponding to the genomic RNA of a South American strain of potato virus X (PVXc) is reported. The sequence (6432 nucleotides) contains five open reading frames coding for polypeptides with molecular weights of 165.3, 24.3, 12.3, 7.6 and 25.0 and displays an overall homology of 77.4% with...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(90)90054-f
更新日期：1990-07-01 00:00:00
abstract：:The ultrastructure of the equine morbillivirus (EMV) which was implicated in the death of one human and fourteen horses in Queensland, Australia during September 1994 and a 36 year old man from Queensland in October 1995 is described. The ultrastructure of the virus and the intracellular virus-specific structures are ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(96)01307-x
更新日期：1996-07-01 00:00:00
abstract：:St. Louis encephalitis virus (SLEV), a flavivirus transmitted to humans by Culex mosquitoes, causes clinical symptoms ranging from acute febrile disorder to encephalitis. To reach the central nervous system (CNS) from circulating blood, the pathogen must cross the blood-brain barrier formed by endothelial cells and as...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.03.005
更新日期：2016-06-02 00:00:00
abstract：:Human papillomaviruses (HPV) are the known cause for a variety of cancers including cervical and epithelial cancers. The cottontail-rabbit papillomavirus (CRPV) serves as a suitable animal model to study the development of these cancers in vivo. We have previously demonstrated that CRPV-induced skin carcinomas express...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.09.007
更新日期：2009-01-01 00:00:00
