当前位置： SCI文献检索 > VIRUS RESEARCH期刊下所有文献 > Structural and functional analysis of the hemagglutinin-esterase of infectious salmon anaemia virus.

Structural and functional analysis of the hemagglutinin-esterase of infectious salmon anaemia virus.

Abstract：

:Infectious salmon anaemia virus (ISAV) is a piscine orthomyxovirus causing a serious disease in farmed Atlantic salmon (Salmo salar L.). The virus surface glycoprotein hemagglutinin-esterase (HE) is responsible for both viral attachment and release. Similarity to bovine and porcine torovirus hemagglutinin-esterase (BToV HE, PToV HE), bovine coronavirus HE (BCoV HE) and influenza C hemagglutinin-esterase-fusion (InfC HEF) proteins were exploited in a computational homology-based structure analysis of ISAV HE. The analysis resolved structural aspects of the protein and identified important features of relevance to ISAV HE activity. By recombinant expression and purification of secretory HE (recHE) proteins, receptor-binding and quantitative analyses of enzymatic activities displayed by ISAV HE molecules are presented for the first time. Three different recHE molecules were constructed: one representing a high virulent isolate, one a low virulent, while in the third a Ser(32) to Ala(32) amino acid substitution was introduced in the enzymatic catalytic site as inferred from the model. The three amino acid differences between the high and low virulent variants, of which two localized to the putative receptor-binding domain and one in the esterase domain, had no impact on receptor-binding or -release activities. In contrast, the Ser(32) amino acid substitution totally abolished enzymatic activity while receptor binding increased, as observed by agglutination of Atlantic salmon red blood cells. This demonstrates the essential role of a serine in the enzyme's catalytic site. In conclusion, structural analysis of ISAV HE in combination with selected recHE proteins gave insights into structure-function relationships and opens up for further studies aiming at dissecting molecular determinants of ISAV virulence.

journal_name

Virus Res

journal_title

Virus research

authors

Müller A,Markussen T,Drabløs F,Gjøen T,Jørgensen TØ,Solem ST,Mjaaland S

doi

10.1016/j.virusres.2010.03.020

subject

Has Abstract

pub_date

2010-08-01 00:00:00

pages

131-41

issue

2

eissn

0168-1702

issn

1872-7492

pii

S0168-1702(10)00123-1

journal_volume

151

pub_type

杂志文章

相关文献

VIRUS RESEARCH文献大全
  • Specific interactions of HeLa cell proteins with Coxsackievirus B3 RNA: La autoantigen binds differentially to multiple sites within the 5' untranslated region.

    abstract：:Translation initiation of the coxsackievirus B3 (CVB3) RNA occurs by internal ribosomal entry. The internal ribosomal entry site (IRES) of the virus has been mapped to the 5' untranslated region (5' UTR) of the genome. As well, the 5' UTR has been suggested to play roles in determining the tissue tropism and infectivi...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/s0168-1702(02)00138-7

    authors： Cheung P,Zhang M,Yuan J,Chau D,Yanagawa B,McManus B,Yang D

    更新日期：2002-12-01 00:00:00

  • The genome sequence and proteome of bacteriophage ΦCPV1 virulent for Clostridium perfringens.

    abstract：:Application of bacteriophages and their lytic enzymes to control Clostridium perfringens is one potential approach to reduce the pathogen on poultry farms and in poultry-processing facilities. Bacteriophages lytic for C. perfringens were isolated from sewage, feces and broiler intestinal contents and ΦCPV1, a virulent...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2010.11.012

    authors： Volozhantsev NV,Verevkin VV,Bannov VA,Krasilnikova VM,Myakinina VP,Zhilenkov EL,Svetoch EA,Stern NJ,Oakley BB,Seal BS

    更新日期：2011-02-01 00:00:00

  • Identification of a murine cell line that distinguishes virulent from attenuated isolates of the morbillivirus Peste des Petits Ruminants, a promising tool for virulence studies.

    abstract：:Comprehensive pathogenesis studies on Peste des Petits Ruminants virus (PPRV) have been delayed so far by the absence of a small animal model reproducing the disease or an in vitro biological system revealing virulence differences. In this study, a mouse 10T1/2 cell line has been identified as presenting different sus...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2020.198035

    authors： Comerlato J,Albina E,Puech C,Franco AC,Minet C,Eloiflin RJ,Rodrigues V,Servan de Almeida R

    更新日期：2020-09-01 00:00:00

  • The peptide encoded by a novel putative lncRNA HBVPTPAP inducing the apoptosis of hepatocellular carcinoma cells by modulating JAK/STAT signaling pathways.

    abstract：:When the hepatitis B virus (HBV) enters target cells, there are complex trans-regulatory mechanisms involved in the interactions between the virus and the target cells. In the present study, a new gene screened from the hepatoblastoma cell line HepG2 using suppression subtractive hybridization, referred to as lncRNA H...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2020.198104

    authors： Lun YZ,Pan ZP,Liu SA,Sun J,Han M,Liu B,Dong W,Pan LH,Cheng J

    更新日期：2020-10-02 00:00:00

  • Detection of RNA in purified cytomegalovirus virions.

    abstract：:Five viral RNA transcripts have recently been detected in purified virions of human cytomegalovirus (HCMV) strain AD169, a well-characterized member of the family Herpesviridae [Science 288 (2000) 2373]. While the function of these transcripts and/or the proteins they encode remains to be elucidated, it is not known w...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2004.03.008

    authors： Sarcinella E,Brown M,Tellier R,Petric M,Mazzulli T

    更新日期：2004-09-01 00:00:00

  • Conditional gene expression in hepatitis C virus transgenic mice without induction of severe liver injury using a non-inflammatory Cre-expressing adenovirus.

    abstract：:We previously established inducible-hepatitis C virus (HCV) transgenic mice, which expressed the HCV gene (nucleotides 294-3435) encoding the core, E1, E2, and NS2 proteins. The expression of these proteins is regulated by the Cre/loxP system and an adenovirus vector (AdV) that expresses Cre DNA recombinase (Cre) cont...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2011.05.019

    authors： Chiyo T,Sekiguchi S,Hayashi M,Tobita Y,Kanegae Y,Saito I,Kohara M

    更新日期：2011-09-01 00:00:00

  • Genetic characterization of Rabies virus isolated from cattle between 1997 and 2002 in an epizootic area in the state of São Paulo, Brazil.

    abstract：:The biogeographical history of rabies can be reconstructed using molecular data. This work describes the genetic characterization of the Rabies virus variant that circulates in the Desmodus rotundus (vampire bat) population in an epizootic area and is transmitted to herbivorous livestock. The N and G genes of this vir...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2009.05.001

    authors： Carnieli P Jr,Castilho JG,Fahl Wde O,Véras NM,Timenetsky Mdo C

    更新日期：2009-09-01 00:00:00

  • Proteins of duck influenza virus responsible for acquisition of pathogenicity in chickens.

    abstract：:Influenza virus rgVac1sub-P0 (H5N1) (rgVac1-P0), in which a pair of dibasic amino acid residues was introduced at the cleavage site of the HA of a reassortant of H5N2 and H7N1 viruses of duck origin, was low pathogenic in chickens. Vac1sub-P3 (H5N1) (Vac1-P3) was selected as a highly pathogenic avian influenza virus b...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2013.02.001

    authors： Yamamoto N,Soda K,Sakoda Y,Okamatsu M,Kida H

    更新日期：2013-05-01 00:00:00

  • Characterization of full genome sequences of chicken anemia viruses circulating in Egypt reveals distinct genetic diversity and evidence of recombination.

    abstract：:Chicken anemia virus (CAV) is one of the commercially important diseases of poultry worldwide. In Egypt, CAV has been reported to be a potential threat to the commercial poultry sectors. Hence, this study was aimed at isolation and full genomic analysis of CAVs circulating in chicken populations in different geographi...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2018.05.008

    authors： Erfan AM,Selim AA,Naguib MM

    更新日期：2018-06-02 00:00:00

  • Live attenuated pseudorabies virus developed using the CRISPR/Cas9 system.

    abstract：:Currently, pseudorabies virus (PRV) variant strains are outbreaking in China; these variants belong to genotype II PRV. The traditional Bartha-K61 vaccine has failed to provide complete protection against the emergent variant strains. Therefore, rapid attenuation of current epidemic strains is needed for effective PRV...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2016.09.004

    authors： Tang YD,Liu JT,Wang TY,An TQ,Sun MX,Wang SJ,Fang QQ,Hou LL,Tian ZJ,Cai XH

    更新日期：2016-10-02 00:00:00

  • The influenza C virus NS gene: evidence for a spliced mRNA and a second NS gene product (NS2 protein).

    abstract：:It has previously been shown that the shortest RNA (RNA 7) of influenza C viruses codes for a nonstructural (NSI) protein (Nakada et al. (1985) J. Virol. 56, 221-226). Experiments reported here indicate that RNA 7 also directs the synthesis of a second nonstructural protein via a spliced mRNA. The amino terminal 62 co...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(86)90005-5

    authors： Nakada S,Graves PN,Palese P

    更新日期：1986-05-01 00:00:00

  • Early intranuclear replication of African swine fever virus genome modifies the landscape of the host cell nucleus.

    abstract：:Although African swine fever virus (ASFV) replicates in viral cytoplasmic factories, the presence of viral DNA within the host cell nucleus has been previously reported to be essential for productive infection. Herein, we described, for the first time, the intranuclear distribution patterns of viral DNA replication ev...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2015.07.006

    authors： Simões M,Martins C,Ferreira F

    更新日期：2015-12-02 00:00:00

  • A natural tetranortriterpenoid with immunomodulating properties as a potential anti-HSV agent.

    abstract：:Meliacine (MA), an antiviral principle present in partially purified leaf extracts of Melia azedarach L., prevents the development of herpetic stromal keratitis (HSK) in mice by diminishing the viral load in the eye and the severity of lesions caused by a virus-induced immunopathological reaction. The tetranortriterpe...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2008.12.013

    authors： Bueno CA,Barquero AA,Di Cónsoli H,Maier MS,Alché LE

    更新日期：2009-04-01 00:00:00

  • The impacts of viral inactivating methods on quantitative RT-PCR for COVID-19.

    abstract：OBJECTIVE:This paper aims to explore the effect of four virus inactivation methods on the rapid detection results of COVID-19 nucleic acid. METHODS:Collected samples of nasopharyngeal swabs from 2 patients diagnosed with COVID-19 at the First People's Hospital of Zhaoqing City, each of sample was divided into 5 groups...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2020.197988

    authors： Wang Y,Song W,Zhao Z,Chen P,Liu J,Li C

    更新日期：2020-08-01 00:00:00

  • Detection of measles virus genome directly from clinical samples by reverse transcriptase-polymerase chain reaction and genetic variability.

    abstract：:A simple and sensitive method for the detection of measles virus genome was developed, amplifying the regions encoding the nucleocapsid (N) protein and hemagglutinin (H) protein of measles virus by reverse transcriptase-polymerase chain reaction (RT-PCR). We examined a variety of measles patients: 28 patients with nat...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(94)00074-m

    authors： Nakayama T,Mori T,Yamaguchi S,Sonoda S,Asamura S,Yamashita R,Takeuchi Y,Urano T

    更新日期：1995-01-01 00:00:00

  • Expression cloning and antigenic analysis of the nucleocapsid protein of equine arteritis virus.

    abstract：:A series of recombinant fusion proteins derived from equine arteritis virus (EAV) open reading frame (ORF) 7 have been used to define the immunoreactive region of the viral nucleocapsid (N) protein. Reactivities of recombinant N fusion proteins with post-infection equine sera in immunoblots and ELISAs indicate that th...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(95)00098-4

    authors： Chirnside ED,Francis PM,Mumford JA

    更新日期：1995-12-01 00:00:00

  • In vitro synthesis of Japanese encephalitis virus (JEV) RNA: membrane and nuclear fractions of JEV-infected cells possess high levels of virus-specific RNA polymerase activity.

    abstract：:Japanese encephalitis virus (JEV)-specific RNAs (including 42S RNA) were synthesized in subcellular fractions prepared from infected C6/36 cells. This in vitro RNA synthesis essentially required Mg2+ and four ribonucleotides, and it was enhanced by K+. The amounts of RNA synthesized in vitro (in extracts from JEV-infe...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(89)90078-6

    authors： Takegami T,Hotta S

    更新日期：1989-08-01 00:00:00

  • Molecular epidemiology of rabies in Indonesia.

    abstract：:In order to clarify the genetic relationships and dynamics of rabies viruses that are epidemic in Indonesia, we determined and analyzed 1307 nucleotides of nucleoprotein genes of 34 rabies field isolates collected from Sumatra, Java, Kalimantan, Sulawesi and Flores islands. Results of phylogenetic analysis indicated t...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2008.03.001

    authors： Susetya H,Sugiyama M,Inagaki A,Ito N,Mudiarto G,Minamoto N

    更新日期：2008-07-01 00:00:00

  • Elevated antigen-specific Th2 type response is associated with the poor prognosis of hand, foot and mouth disease.

    abstract：:The immunopathogenesis of severe hand, foot and mouth disease (HFMD) remains elusive. This study revealed that enterovirus 71 (EV71) epitope-specific CD4+ T cell responses of HFMD patients were skewed toward a Th2 cytokine profile. Patients that demonstrated higher levels of IL-4 expression in their CD4 T cells follow...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2013.07.009

    authors： Wei R,Xu L,Zhang N,Zhu K,Yang J,Yang C,Deng C,Zhu Z,De Groot AS,Altmeyer R,Zeng M,Leng Q

    更新日期：2013-10-01 00:00:00

  • Complete genome sequence of a raccoon rabies virus isolate.

    abstract：:The entire genome of a mid-Atlantic raccoon strain rabies virus (RRV) isolated in Canada was sequenced; this is the second North American wildlife rabies virus isolate to be fully characterized. The overall organization and length of the genome was similar to that of other lyssaviruses. The nucleotide sequence identit...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2008.04.029

    authors： Szanto AG,Nadin-Davis SA,White BN

    更新日期：2008-09-01 00:00:00

  • Impaired cardiac microvascular endothelial cells function induced by Coxsackievirus B3 infection and its potential role in cardiac fibrosis.

    abstract：:CVB3 virus tropism and tissue access are modulated by cardiac microvascular endothelial cells (CMVECs) in the context of microvasculature. This study was designed to examine biological behaviors of CMVECs following CVB3 infection and its possible effects on cardiac remodeling. Data demonstrated that CVB3 increased cas...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2012.07.027

    authors： Xie Y,Liao J,Li M,Wang X,Yang Y,Ge J,Chen R,Chen H

    更新日期：2012-10-01 00:00:00

  • Australian monocot-infecting mastrevirus diversity rivals that in Africa.

    abstract：:Monocotyledonous plant infecting mastreviruses (family Geminiviridae) have been found in the Old World. The greatest diversity of these viruses has been found in Africa but this may simply reflect the more extensive sampling that has been done there. To provide a better understanding of mastrevirus diversity in Austra...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2012.07.018

    authors： Kraberger S,Thomas JE,Geering AD,Dayaram A,Stainton D,Hadfield J,Walters M,Parmenter KS,van Brunschot S,Collings DA,Martin DP,Varsani A

    更新日期：2012-10-01 00:00:00

  • The pathogenic and vaccine strains of equine infectious anemia virus differentially induce cytokine and chemokine expression and apoptosis in macrophages.

    abstract：:The attenuated equine infectious anemia virus (EIAV) vaccine was the first attenuated lentivirus vaccine to be used in a large-scale application and has been used to successfully control the spread of equine infectious anemia (EIA) in China. To better understand the potential role of cytokines in the pathogenesis of E...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2011.06.028

    authors： Lin YZ,Cao XZ,Li L,Li L,Jiang CG,Wang XF,Ma J,Zhou JH

    更新日期：2011-09-01 00:00:00

  • Beta-actin interacts with the E2 protein and is involved in the early replication of classical swine fever virus.

    abstract：:The E2 glycoprotein of classical swine fever virus (CSFV) is involved in viral infection and induction of neutralizing antibodies. Little is known about how E2 interacts with host cells. To understand the cellular factors involved in viral replication cycle, E2 was used as bait in yeast two-hybrid screens, resulting i...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2013.10.016

    authors： He F,Ling L,Liao Y,Li S,Han W,Zhao B,Sun Y,Qiu HJ

    更新日期：2014-01-22 00:00:00

  • USP7 manipulation by viral proteins.

    abstract：:Ubiquitin Specific Protease 7 (USP7) is a deubiquitinating enzyme (DUB) that plays critical roles in the regulation of many cellular processes including epigenetics, tumour suppression, oncogenesis, DNA damage response, immunity and viral infection. USP7 was the first DUB associated with viral infection. Since then ot...

    journal_title：Virus research

    pub_type： 杂志文章,评审

    doi：10.1016/j.virusres.2020.198076

    authors： Bojagora A,Saridakis V

    更新日期：2020-09-01 00:00:00

  • Structure of hepatitis E viral particle.

    abstract：:Hepatitis E is acute hepatitis caused by infection of hepatitis E virus (HEV) via a fecal-to-oral or zoonotic route. HEV is a small, non-enveloped virus containing positive strand RNA as a genome. Recently, the three-dimensional structures of the HEV-like particles and spike domain protruded from the surface of the pa...

    journal_title：Virus research

    pub_type： 杂志文章,评审

    doi：10.1016/j.virusres.2011.03.015

    authors： Mori Y,Matsuura Y

    更新日期：2011-10-01 00:00:00

  • Increase of virus yields and releases of Borna disease virus from persistently infected cells.

    abstract：:Borna disease virus grows to low titres in persistently infected cells with an infectious particle to cell ratio of 0.01 to 0.05. Inclusion of n-butyrate in the growth medium enhances infectivity yields up to 1 log. This effect is time and concentration dependent. In hypertonic medium with an excess of NaCl, KCl or Na...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(85)90057-7

    authors： Pauli G,Ludwig H

    更新日期：1985-02-01 00:00:00

  • Mutation of neutralizing/antibody-dependent enhancing epitope on spike protein and 7b gene of feline infectious peritonitis virus: influences of viral replication in monocytes/macrophages and virulence in cats.

    abstract：:We previously prepared neutralizing monoclonal antibody (MAb)-resistant (mar) mutant viruses using a laboratory strain feline infectious peritonitis virus (FIPV) 79-1146 (Kida et al., 1999). Mar mutant viruses are mutated several amino acids of the neutralizing epitope of Spike protein, compared with the parent strain...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2010.12.020

    authors： Takano T,Tomiyama Y,Katoh Y,Nakamura M,Satoh R,Hohdatsu T

    更新日期：2011-03-01 00:00:00

  • Complete genome analysis of dengue virus type 3 isolated from the 2013 dengue outbreak in Yunnan, China.

    abstract：:In the past few decades, dengue has spread rapidly and is an emerging disease in China. An unexpected dengue outbreak occurred in Xishuangbanna, Yunnan, China, resulting in 1331 patients in 2013. In order to obtain the complete genome information and perform mutation and evolutionary analysis of causative agent relate...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2017.06.015

    authors： Wang X,Ma D,Huang X,Li L,Li D,Zhao Y,Qiu L,Pan Y,Chen J,Xi J,Shan X,Sun Q

    更新日期：2017-06-15 00:00:00

  • Characterization of self-assembled virus-like particles of dromedary camel hepatitis e virus generated by recombinant baculoviruses.

    abstract：:Dromedary camel hepatitis E virus (DcHEV), a novel hepatitis E virus, has been identified in dromedary camels in Dubai, United Arab Emirates. The antigenicity, pathogenicity and epidemiology of this virus have been unclear. Here we first used a recombinant baculovirus expression system to express the 13 and 111 N-term...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2015.06.022

    authors： Zhou X,Kataoka M,Liu Z,Takeda N,Wakita T,Li TC

    更新日期：2015-12-02 00:00:00