Abstract:
:A series of recombinant fusion proteins derived from equine arteritis virus (EAV) open reading frame (ORF) 7 have been used to define the immunoreactive region of the viral nucleocapsid (N) protein. Reactivities of recombinant N fusion proteins with post-infection equine sera in immunoblots and ELISAs indicate that the major nucleocapsid protein epitope is located within amino acid residues 1-69. In ELISAs two recombinant nucleocapsid fusion proteins containing residues 1-69 (rN1-69) and 1-28 (rN1-28) discriminated between pre- and post-infection, and pre- and post-vaccination serum samples. Additionally rN1-69 and rN1-28 detected seroconversions following vaccination with a killed virus preparation, even in the absence of a detectable virus neutralising response. Although a good correlation existed between virus neutralising antibody and rN1-69 ELISA positive values in post-infection sera, all the rN proteins failed to induce any virus neutralising response in immunised rabbits.
journal_name
Virus Resjournal_title
Virus researchauthors
Chirnside ED,Francis PM,Mumford JAdoi
10.1016/0168-1702(95)00098-4subject
Has Abstractpub_date
1995-12-01 00:00:00pages
277-88issue
2-3eissn
0168-1702issn
1872-7492pii
0168-1702(95)00098-4journal_volume
39pub_type
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