Abstract：
:Japanese encephalitis virus (JEV)-specific RNAs (including 42S RNA) were synthesized in subcellular fractions prepared from infected C6/36 cells. This in vitro RNA synthesis essentially required Mg2+ and four ribonucleotides, and it was enhanced by K+. The amounts of RNA synthesized in vitro (in extracts from JEV-infected cells) increased as a function of time after infection. The RNA-synthetic activity in nuclear fractions was the highest among three kinds of subcellular fractions. Our data showed that nonstructural proteins NS3 and NS5 were membrane-associated proteins. In particular, NS3 was found almost exclusively in the nuclear and membrane fractions. Our results suggest that NS5 and NS3 may play specific role(s) in flavivirus RNA replication.
journal_name
Virus Resjournal_title
Virus researchauthors
Takegami T,Hotta Sdoi
10.1016/0168-1702(89)90078-6subject
Has Abstractpub_date
1989-08-01 00:00:00pages
337-50issue
4eissn
0168-1702issn
1872-7492pii
0168-1702(89)90078-6journal_volume
13pub_type
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