Abstract：
:The CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9) system has been widely used for viral genome editing, transcription regulation and chromosomal localization in eukaryotic cells. In this study, a guide RNA (gRNA) that specifically recognizes HSV-1 viral genomes was used in the CRISPR-Cas9 system to inhibit viral replication. This inhibition could be achieved with both wild type Cas9 protein and Cas9 nickase (D10A). By targeting viral genomes containing sequences recognized by the gRNA, the CRISPR-Cas9 system distinguished between different viral genome sequences and provided single nucleotide-specific selection pressure to significantly change the proportions of viruses in a mixed viral pool. This finding indicates the utility of this tool for virus selection without the need for antibiotics or reporter genes, which could potentially save time compared to other methods used for screening and purifying mutant viruses.
journal_name
Virus Resjournal_title
Virus researchauthors
Li Z,Bi Y,Xiao H,Sun L,Ren Y,Li Y,Chen C,Cun Wdoi
10.1016/j.virusres.2017.03.010subject
Has Abstractpub_date
2018-01-15 00:00:00pages
286-295eissn
0168-1702issn
1872-7492pii
S0168-1702(16)30694-3journal_volume
244pub_type
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