AP-1 and ERK1 but not p38 nor JNK is required for CRPV early protein 2-dependent MMP-9 promoter activation in rabbit epithelial cells.


:Human papillomaviruses (HPV) are the known cause for a variety of cancers including cervical and epithelial cancers. The cottontail-rabbit papillomavirus (CRPV) serves as a suitable animal model to study the development of these cancers in vivo. We have previously demonstrated that CRPV-induced skin carcinomas express high levels of MMP-9, a metalloproteinase contributing to cancer progression by extracellular matrix remodelling. Based on our previously reported finding that CRPV early protein 2 can activate a truncated human 670bp MMP-9 promoter fragment, we hypothesized that MMP-9 expression in the rabbit carcinomas is a consequence of activation of the rabbit MMP-9 promoter in-trans by CRPV early protein 2. Further elucidation of the mechanism revealed the requirement for both a proximal and distal AP-1 transcription factor binding site in the rabbit MMP-9 promoter and the AP-1 complex as demonstrated by the inhibitory effect of TAM67, a trans-activation deficient c-jun mutant. The characterization of signal-transduction requirements revealed predominantly ERK1 to be required for CRPV early protein 2-dependent MMP-9 promoter activation, but not JNK nor p38. In summary CRPV early protein 2 activates the expression of MMP-9 in-trans through AP-1 and ERK1 and may contribute to cancer development and progression via this mechanism within the animal model.


Virus Res


Virus research


Mühlen S,Behren A,Iftner T,Plinkert PK,Simon C




Has Abstract


2009-01-01 00:00:00














  • Effect of recombinant hybrid human interferon on replication and morphogenesis of HSV-1 in monkey cells.

    abstract::Human recombinant alpha interferon, A/D, significantly reduced the replication and cell fusion induced by herpes simplex virus type 1 in monkey cells. Thin-section electron microscopy of interferon-treated monkey cells showed distinct assembly of nucleocapsids within the nucleus. Analysis of virus-specific proteins by...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Chatterjee S,Whitley RJ

    更新日期:1989-01-01 00:00:00

  • Rapamycin enhances lytic replication of Epstein-Barr virus in gastric carcinoma cells by increasing the transcriptional activities of immediate-early lytic promoters.

    abstract::Epstein-Barr virus (EBV), a human herpesvirus, is linked to both epithelial and lymphoid malignancies. Induction of EBV reactivation is a potential therapeutic strategy for EBV-associated tumors. In this study, we assessed the effects of rapamycin on EBV reactivation in gastric carcinoma cells. We found that rapamycin...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Wang M,Wu W,Zhang Y,Yao G,Gu B

    更新日期:2018-01-15 00:00:00

  • Induction of the intrinsic apoptotic pathway in cells infected with cytopathic bovine virus diarrhoea virus.

    abstract::Cytopathic bovine viral diarrhoea virus (cp BVDV) induces apoptosis in bovine cell cultures. This also seems to be a prominent feature in the pathogenesis of mucosal disease. To gain an insight into the molecular pathways of the cell alterations, the involvement of different members of the apoptotic cascade was analyz...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Grummer B,Bendfeldt S,Wagner B,Greiser-Wilke I

    更新日期:2002-12-01 00:00:00

  • Bovine herpesvirus-1: Genetic diversity of field strains from cattle with respiratory disease, genital, fetal disease and systemic neonatal disease and their relationship to vaccine strains.

    abstract::Bovine herpesvirus-1 (BoHV-1) causes disease in cattle with varied clinical forms. In the U.S. there are two BoHV1 subtypes, BoHV-1.1 and BoHV-1.2b. Control programs in North America incorporate modified live (MLV) or killed (KV) viral vaccines. However, BoHV-1 strains continue to be isolated from diseased animals or ...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Fulton RW,d'Offay JM,Dubovi EJ,Eberle R

    更新日期:2016-09-02 00:00:00

  • The pathogenic and vaccine strains of equine infectious anemia virus differentially induce cytokine and chemokine expression and apoptosis in macrophages.

    abstract::The attenuated equine infectious anemia virus (EIAV) vaccine was the first attenuated lentivirus vaccine to be used in a large-scale application and has been used to successfully control the spread of equine infectious anemia (EIA) in China. To better understand the potential role of cytokines in the pathogenesis of E...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Lin YZ,Cao XZ,Li L,Li L,Jiang CG,Wang XF,Ma J,Zhou JH

    更新日期:2011-09-01 00:00:00

  • Temporal evolutionary analysis of re-emerging recombinant GII.P16_GII.2 norovirus with acute gastroenteritis in patients from Hubei Province of China, 2017.

    abstract::Norovirus (NoV) is a major pathogenic agent of human acute viral gastroenteritis that occurs worldwide. In March 2017, a series of acute NoV-associated gastroenteritis outbreaks occurred in Hubei Province in central China. Here, we sought to better understand the main genotypes and potential evolutionary advantages of...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Li J,Zhang T,Cai K,Jiang Y,Guan X,Zhan J,Zou W,Yang Z,Xing X,Wu Y,Song Y,Yu X,Xu J

    更新日期:2018-04-02 00:00:00

  • Conservation and polymorphism of EBV RPMS1 gene in EBV-associated tumors and healthy individuals from endemic and non-endemic nasopharyngeal carcinoma areas in China.

    abstract::As a member of the BamHI-A rightward transcripts family of the Epstein-Barr virus (EBV), RPMS1 expression has been confirmed in all EBV-associated tumors. However, few studies have investigated the single-nucleotide polymorphisms (SNPs) of RPMS1, and only one SNP site (g155391a) has been reported to be associated with...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Wu S,Liu W,Li H,Zhao Z,Yang Y,Xiao H,Song Y,Luo B

    更新日期:2018-05-02 00:00:00

  • A natural tetranortriterpenoid with immunomodulating properties as a potential anti-HSV agent.

    abstract::Meliacine (MA), an antiviral principle present in partially purified leaf extracts of Melia azedarach L., prevents the development of herpetic stromal keratitis (HSK) in mice by diminishing the viral load in the eye and the severity of lesions caused by a virus-induced immunopathological reaction. The tetranortriterpe...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Bueno CA,Barquero AA,Di Cónsoli H,Maier MS,Alché LE

    更新日期:2009-04-01 00:00:00

  • Characterization of a new megabirnavirus that confers hypovirulence with the aid of a co-infecting partitivirus to the host fungus, Rosellinia necatrix.

    abstract::A new virus termed Rosellinia necatrix megabirnavirus 2 (RnMBV2) was molecularly and biologically characterized. RnMBV2 was originally harbored in isolate W8 of R. necatrix co-infected with the previously reported virus Rosellinia necatrix partitivirus 1 (RnPV1). RnMBV2 has molecular features similar and different fro...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Sasaki A,Nakamura H,Suzuki N,Kanematsu S

    更新日期:2016-07-02 00:00:00

  • Detection of measles virus genome directly from clinical samples by reverse transcriptase-polymerase chain reaction and genetic variability.

    abstract::A simple and sensitive method for the detection of measles virus genome was developed, amplifying the regions encoding the nucleocapsid (N) protein and hemagglutinin (H) protein of measles virus by reverse transcriptase-polymerase chain reaction (RT-PCR). We examined a variety of measles patients: 28 patients with nat...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Nakayama T,Mori T,Yamaguchi S,Sonoda S,Asamura S,Yamashita R,Takeuchi Y,Urano T

    更新日期:1995-01-01 00:00:00

  • Incorporation fidelity of the viral RNA-dependent RNA polymerase: a kinetic, thermodynamic and structural perspective.

    abstract::Positive-strand RNA viruses exist as a quasi-species due to the incorporation of mutations into the viral genome during replication by the virus-encoded RNA-dependent RNA polymerase (RdRP). Therefore, the RdRP is often described as a low-fidelity enzyme. However, until recently, a complete description of the kinetic, ...

    journal_title:Virus research

    pub_type: 杂志文章,评审


    authors: Castro C,Arnold JJ,Cameron CE

    更新日期:2005-02-01 00:00:00

  • Evolutionary and phylodynamic analyses of Dengue virus serotype I in Guangdong Province, China, between 1985 and 2015.

    abstract::To determine the evolutionary and phylodynamic history of DENV-1 in Guangdong, the strains detected between 1985 and 2015 were determined with phylogenetic and Bayesian analyses of the E gene. Three DENV-1 genotypes (I, V, and VI) were circulating in Guangdong, and genotype I was detected most frequently. The evolutio...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Bai Z,Liu LC,Jiang L,Luo L,Feng H,Lin P,Jing Q,Xiao X,Zhou H,Su W,Cao Y,Li Y,Cao Q,Chen W,Di B,Yang Z

    更新日期:2018-09-02 00:00:00

  • Beta-actin interacts with the E2 protein and is involved in the early replication of classical swine fever virus.

    abstract::The E2 glycoprotein of classical swine fever virus (CSFV) is involved in viral infection and induction of neutralizing antibodies. Little is known about how E2 interacts with host cells. To understand the cellular factors involved in viral replication cycle, E2 was used as bait in yeast two-hybrid screens, resulting i...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: He F,Ling L,Liao Y,Li S,Han W,Zhao B,Sun Y,Qiu HJ

    更新日期:2014-01-22 00:00:00

  • Analysis of codon usage bias in potato virus Y non-recombinant strains.

    abstract::Potato virus Y (PVY) is a member of the genus Potyvirus, family Potyviridae, is considered one of the most devastating pest affecting economically important crops, such as potato, tobacco, tomato and pepper, representing a serious threat due to high incidence and worldwide distribution. Its economic significance as we...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Gómez MM,de Mello Volotão E,Assandri IR,Peyrou M,Cristina J

    更新日期:2020-09-01 00:00:00

  • New developments in tracing neural circuits with herpesviruses.

    abstract::Certain neurotropic viruses can invade the nervous system of their hosts and spread in chains of synaptically connected neurons. Consequently, it is possible to identify entire hierarchically connected circuits within an animal. In this review, we discuss the use of neurotropic herpesviruses as neuronal tract tracers....

    journal_title:Virus research

    pub_type: 杂志文章,评审


    authors: Song CK,Enquist LW,Bartness TJ

    更新日期:2005-08-01 00:00:00

  • Molecular analysis of double-stranded RNAs reveals complex infection of grapevines with multiple viruses.

    abstract::The table grape variety "Waltham Cross" was infected with Leafroll and Shiraz Disease. To reveal specific viruses that are associated with the diseased plants, we used an RT-PCR-based strategy to determine partial genome sequences of these viruses. Upon cloning and sequencing of the RT-PCR products, we detected seven ...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Prosser SW,Goszczynski DE,Meng B

    更新日期:2007-03-01 00:00:00

  • Plant subviral RNAs as a long noncoding RNA (lncRNA): Analogy with animal lncRNAs in host-virus interactions.

    abstract::Satellite RNAs (satRNAs) and viroids belong to the group called subviral agents and are the smallest pathogens of plants. In general, small satRNAs and viroids are 300-400 nt in size and do not encode any functional proteins; they are thus regarded as so-called long noncoding RNAs (lncRNAs). These lncRNAs are receivin...

    journal_title:Virus research

    pub_type: 杂志文章,评审


    authors: Shimura H,Masuta C

    更新日期:2016-01-02 00:00:00

  • Mutation of neutralizing/antibody-dependent enhancing epitope on spike protein and 7b gene of feline infectious peritonitis virus: influences of viral replication in monocytes/macrophages and virulence in cats.

    abstract::We previously prepared neutralizing monoclonal antibody (MAb)-resistant (mar) mutant viruses using a laboratory strain feline infectious peritonitis virus (FIPV) 79-1146 (Kida et al., 1999). Mar mutant viruses are mutated several amino acids of the neutralizing epitope of Spike protein, compared with the parent strain...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Takano T,Tomiyama Y,Katoh Y,Nakamura M,Satoh R,Hohdatsu T

    更新日期:2011-03-01 00:00:00

  • Naturally occurring NS3 resistance-associated variants in hepatitis C virus genotype 1: Their relevance for developing countries.

    abstract::Hepatitis C virus (HCV) is a major cause of global morbidity and mortality, with an estimated 130-150 million infected individuals worldwide. HCV is a leading cause of chronic liver diseases including cirrhosis and hepatocellular carcinoma. Current treatment options in developing countries involve pegylated interferon...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Echeverría N,Betancour G,Gámbaro F,Hernández N,López P,Chiodi D,Sánchez A,Boschi S,Fajardo A,Sóñora M,Moratorio G,Cristina J,Moreno P

    更新日期:2016-09-02 00:00:00

  • Human group C rotavirus: completion of the genome sequence and gene coding assignments of a non-cultivatable rotavirus.

    abstract::Genome segments 1 and 2 of human group C rotavirus 'Bristol' strain were sequenced and their gene-protein coding properties assigned. This work completed the genome sequence of a human group C rotavirus (17,910 bp) and allowed the full gene-protein coding assignment of the 11 segments of dsRNA. Gene 1 is 3309 bp in si...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Chen Z,Lambden PR,Lau J,Caul EO,Clarke IN

    更新日期:2002-02-26 00:00:00

  • Interferon-mediated inhibition of retroviral infection: use of a defective retrovirus carrying a drug-resistance gene.

    abstract::We here report the results of an investigation of the effect of interferon on the establishment of new infections by a retrovirus. For this study, we used an infectious but replication-incompetent retrovirus carrying a drug-resistance gene and assayed for infectivity by measuring drug-resistant colony formation. Mouse...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Kumar R,Sen GC

    更新日期:1989-08-01 00:00:00

  • A contribution to the systematization of bovine herpesvirus 1 based on genomic mapping by restriction fragment pattern analysis.

    abstract::Fourteen isolates of bovine herpesvirus 1 (BHV-1) found representative of more than 100 isolates studied, were compared by restriction fragment pattern analyses and molecularly characterized. A number of evolutionary links between the variants originally associated with infectious bovine rhinotracheitis and the varian...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Christensen LS,Madsen KG,Nylin B,Rønsholt L

    更新日期:1996-12-01 00:00:00

  • Genome analysis of an orange stem pitting citrus tristeza virus isolate reveals a novel recombinant genotype.

    abstract::An orange stem pitting citrus tristeza virus (CTV) isolate CTV-B165 was found to be symptomatically similar to other known CTV-VT isolates however molecular methods failed to classify it as an identifiable CTV genotype. The sequence variation of the Indian CTV-B165 isolate was compared to the three well known CTV geno...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Roy A,Brlansky RH

    更新日期:2010-08-01 00:00:00

  • Identification of a protein encoded in the herpes simplex virus type 1 latency associated transcript promoter region.

    abstract::Herpes simplex virus type 1 (HSV-1) establishes a lifelong latency in the neurons of its host. Sporadically, the latent virus reactivates and spreads back to the original site of infection and causes recrudescent diseases. The only gene actively transcribed during neuronal latency is the latency associated transcript ...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Naito J,Mukerjee R,Mott KR,Kang W,Osorio N,Fraser NW,Perng GC

    更新日期:2005-03-01 00:00:00

  • Determination of the mutation rate of poliovirus RNA-dependent RNA polymerase.

    abstract::The fidelity of poliovirus RNA-dependent RNA polymerase (3D(pol)) was determined using a system based on the fidelity of synthesis of the alpha-lac gene which codes for a subunit of beta-galactosidase. Synthesis products are screened for mutations by an alpha-complementation assay, in which the protein product from al...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Wells VR,Plotch SJ,DeStefano JJ

    更新日期:2001-04-01 00:00:00

  • Expression cloning and antigenic analysis of the nucleocapsid protein of equine arteritis virus.

    abstract::A series of recombinant fusion proteins derived from equine arteritis virus (EAV) open reading frame (ORF) 7 have been used to define the immunoreactive region of the viral nucleocapsid (N) protein. Reactivities of recombinant N fusion proteins with post-infection equine sera in immunoblots and ELISAs indicate that th...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Chirnside ED,Francis PM,Mumford JA

    更新日期:1995-12-01 00:00:00

  • Heterogeneity in spike protein genes of porcine epidemic diarrhea viruses isolated in Korea.

    abstract::Porcine epidemic diarrhea virus (PEDV) has plagued the domestic swine industry in Korea causing significant economic impacts on pig production nationwide. In the present study, we determined the complete nucleotide sequences of the spike (S) glycoprotein genes of seven Korean PEDV isolates. The entire S genes of all i...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Lee DK,Park CK,Kim SH,Lee C

    更新日期:2010-05-01 00:00:00

  • African swine fever virus-induced polypeptides in Vero cells.

    abstract::African swine fever virus induces in Vero cells 81 acid and 14 basic polypeptides which account for most of the information content of the virus DNA. The kinetics and the cytosine arabinoside sensitivity of the synthesis of the virus-induced polypeptides showed the existence of three classes of proteins, two early and...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: Santarén JF,Viñuela E

    更新日期:1986-09-01 00:00:00

  • RNA recombination in hepatitis delta virus: implications regarding the abilities of mammalian RNA polymerases.

    abstract::Hepatitis delta virus (HDV) requires the surface antigens of hepatitis B virus (HBV) for packaging and transmission, but replicates its RNA in an HBV-independent fashion. HDV contains a 1.7-kb circular RNA genome that is folded into an unbranched rod-like structure via intramolecular base-pairing, and possesses ribozy...

    journal_title:Virus research

    pub_type: 杂志文章,评审


    authors: Chao M

    更新日期:2007-08-01 00:00:00

  • The cytoplasmic tail of the neuraminidase protein of influenza A virus does not play an important role in the packaging of this protein into viral envelopes.

    abstract::We have rescued a transfectant influenza virus, NA/TAIL(-), whose neuraminidase (NA) protein lacks the predicted cytoplasmic tail. The virus was attenuated (one log10 reduction) both in tissue culture and in mouse lungs. Attenuation correlated with a 50% reduction of the level of NA in infected cells and levels of inc...

    journal_title:Virus research

    pub_type: 杂志文章


    authors: García-Sastre A,Palese P

    更新日期:1995-06-01 00:00:00