Abstract:
:Fowlpox virus (FWPV) is currently used as a vector to express foreign genes of various poultry and mammalian pathogens. However, due to limited information available about the primary structure of FWPV promoters required for an optimal transcriptional efficiency, the full potential of FWPV as an expression vector has not been completely realized. To dissect such transcriptional regulatory elements at the molecular level, we selected two FWPV promoters dictating contrasting levels of expression of acidic-type inclusion body protein gene (P190) and A15L vaccinia virus homolog of FWPV (P180) for site-directed mutagenesis studies. The transcriptional activity of mutated promoters was analyzed based on their ability to transcribe a reporter gene, lacZ, and translation of the resultant mRNA into functional protein. Replacement of the spacer sequences of P180 with those of P190 resulted in a five-fold increase in mRNA and a 17.6-fold increase in protein over those with its parental promoter, P180. Similarly, replacement of a thymidine after the start codon with guanosine resulted in a 2.3-fold increase in lacZ mRNA and a seven-fold increase in protein. Combining these substitutions in P180SG produced a maximum increase in mRNA and protein of 6.7- and 29.9-fold, respectively, over concentrations with its parental P180 promoter. The promoter activity of P180SG was comparable to that of the strongest natural promoter, P190. The amount of protein per transcript generated by the mutated promoters of P180 increased to at least three times that with the parental P180. In contrast, similar replacements in P190 resulted in a 40-50% reduction in mRNA and protein in all the mutated promoters. We discuss the significance of spacer sequence and the purine after the start codon in the context of a high level of expression.
journal_name
Virus Resjournal_title
Virus researchauthors
Srinivasan V,Schnitzlein WM,Tripathy DNdoi
10.1016/j.virusres.2005.08.017subject
Has Abstractpub_date
2006-03-01 00:00:00pages
85-90issue
1-2eissn
0168-1702issn
1872-7492pii
S0168-1702(05)00267-4journal_volume
116pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract::Raccoons (Procyon lotor), which are not native to Japan, have been suspected to transmit various pathogens by frequent intrusion into agricultural and residential areas. To determine influenza A virus seropositivity in raccoons in Japan, we examined a total of 634 raccoons captured in 19 towns (A-S) from 2009 to 2012....
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2014.05.004
更新日期:2014-08-30 00:00:00
abstract::Persistent infections with mumps virus were established in human B-lymphoid cell line Akata and in the human chronic myelogenous leukaemia cell line K562. Even after IFN treatment a drastic decrease in STAT-1alpha (signal transducers and activators of transcription-1alpha), STAT-2 and p48 (ISGF-3gamma: IFN-stimulated ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(99)00114-8
更新日期:1999-12-15 00:00:00
abstract::Pseudorabies virus (PrV), a member of herpesviridae alphaherpes subfamily, can infect human cells in vitro. However, the transmission to Old World primates including humans is strongly restricted. In this study, we report the neutralizing activity of normal human serum against PrV grown in CPK cells derived from pig. ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2003.09.008
更新日期:2004-01-01 00:00:00
abstract::Primers corresponding to conserved regions in the RNA-dependent RNA polymerase and the RACE procedure led to the cloning of the complete sweetpotato mild mottle virus (SPMMV) RNA genome. The assembled SPMMV genomic sequence was 10,818 nucleotides in length with a polyadenylated tract at the 3' terminus. The structure ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(97)00148-2
更新日期:1998-02-01 00:00:00
abstract::Herpes simplex virus glycoprotein B (HSV gB) is essential for penetration of virus into cells, for cell-to-cell spread of virus, and for cell-cell fusion. Every member of the family Herpesviridae has a gB homolog, underlining its importance. The antigenic structure of gB has been studied extensively, but little is kno...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(98)00030-6
更新日期:1998-05-01 00:00:00
abstract::Translation initiation of the coxsackievirus B3 (CVB3) RNA occurs by internal ribosomal entry. The internal ribosomal entry site (IRES) of the virus has been mapped to the 5' untranslated region (5' UTR) of the genome. As well, the 5' UTR has been suggested to play roles in determining the tissue tropism and infectivi...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(02)00138-7
更新日期:2002-12-01 00:00:00
abstract::I have observed a relationship between GC content in coding sequences of RNA viruses and their genome polarity. Positive-stranded RNA viruses have significantly higher GC contents than negative-stranded RNA viruses. Coding sequences of all negative-stranded RNA viruses are biased toward high A in coding strands (high ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2004.10.004
更新日期:2005-04-01 00:00:00
abstract::To evaluate the mechanism by which a large outbreak of porcine epidemic diarrhea (PED) occurred in Japan, where the majority of sows are vaccinated, we isolated two new strains of PED virus (PEDV) from the intestines of piglets and found that they showed greater similarity to US isolates (group II PEDV) than to the Ja...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2016.06.006
更新日期:2016-08-15 00:00:00
abstract::Tomato spotted wilt virus (TSWV) is a plant-infecting member of the family Bunyaviridae. TSWV encodes two envelope glycoproteins, G(N) and G(C), which are required for virus infection of the arthropod vector. Other members of the Bunyaviridae enter host cells by pH-dependent endocytosis. During this process, the glyco...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2005.01.007
更新日期:2005-06-01 00:00:00
abstract::An account is given of progress during the last 30 years in knowledge of the epidemiology of diseases caused by aphid-borne viruses in seed potato production areas of northern Europe. During this period, Potato leafroll virus (PLRV) and strains of Potato virus Y (PVY(O), PVY(N)) were consistently the most prevalent an...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/s0168-1702(00)00186-6
更新日期:2000-11-01 00:00:00
abstract::Puumala (PUU) virus causes a form of hemorrhagic fever with renal syndrome (HFRS), called nephropathia epidemica (NE), in Europe. HFRS is characterized by an increased capillary permeability, which we hypothesize is caused by hyperactivation of the host immune system, especially cellular immune responses. To identify ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(01)00416-6
更新日期:2002-03-20 00:00:00
abstract::A study of the evolutionary relationships of GLRaV-4,-5,-6 and -9, and two new Ampelovirus isolates (GLRaV-Pr and -De) related to grapevine leafroll disease was conducted based on molecular variability, positive selection analysis and maximum likelihood phylogenetic reconstructions. Sequences corresponding to the N-te...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2008.02.015
更新日期:2008-07-01 00:00:00
abstract::Murine cytomegalovirus (MCMV) m137 null mutants, Deltam137A and Deltam137B, were generated by inserting a gpt cassette into a deleted region of the open reading frame. A polyclonal antiserum produced to an Escherichia coli expressed gst-m137 fusion protein was used to show that a 38 kDa polypeptide corresponding to th...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(02)00024-2
更新日期:2002-03-20 00:00:00
abstract::The T-helper response to the measles virus nucleoprotein (NP) has been studied in mice. The T-cell proliferative response was measured in lymphocytes from mice immunized with either a vaccinia measles-NP recombinant virus or a mouse neuro-adapted measles virus. A T-cell response was obtained with lymphocytes from H2d ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(92)90088-q
更新日期:1992-01-01 00:00:00
abstract::Noroviruses belong to the Caliciviridae family of single-stranded positive-sense RNA viruses. The genus Norovirus includes seven genogroups (designated GI-GVII), of which GI, GII and GIV infect humans. Human noroviruses are responsible for widespread outbreaks of acute gastroenteritis and represent one of the most com...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2016.12.018
更新日期:2017-04-15 00:00:00
abstract::As a member of the BamHI-A rightward transcripts family of the Epstein-Barr virus (EBV), RPMS1 expression has been confirmed in all EBV-associated tumors. However, few studies have investigated the single-nucleotide polymorphisms (SNPs) of RPMS1, and only one SNP site (g155391a) has been reported to be associated with...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2018.04.009
更新日期:2018-05-02 00:00:00
abstract::Recent scientific advances are providing an opportunity to revisit strategies for cervical cancer prevention. How to invest health resources wisely, such that clinical benefits are maximized-and opportunity costs are minimized-is a critical question in the setting of new technology. In addition to an intervention's ef...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/s0168-1702(02)00199-5
更新日期:2002-11-01 00:00:00
abstract::A temperature-sensitive mutant of the Moloney murine leukemia virus-TB, ts1, causes hindlimb paralysis and immunodeficiency in mice. At the restrictive temperature, the envelope precursor polyprotein, gPr80env, is inefficiently processed intracellularly, and this is associated with the neurovirulence of ts1. To test t...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(91)90096-e
更新日期:1991-03-01 00:00:00
abstract::The coat protein (CP) is the only structural protein present in the polyprotein of bean common mosaic virus. The well known characteristics of the CP are self-oligomerization and nucleic acid binding activity. The studies of the coat protein mutants revealed that the oligomeric property of CP solely depends on the ami...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2019.197755
更新日期:2019-11-01 00:00:00
abstract::Here we document that the gene VI product of peanut chlorotic streak virus (PClSV), a newly characterized member of the group, transactivates the translation of dicistronic transcripts. Dicistronic expression units have been analyzed both in protoplast transient expression experiments and in transgenic tobacco plants....
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(98)00088-4
更新日期:1998-10-01 00:00:00
abstract::Highly pathogenic avian H5N1 influenza A viruses occasionally infect humans and cause severe respiratory disease and fatalities. Currently, these viruses are not efficiently transmitted from person to person, although limited human-to-human transmission may have occurred. Nevertheless, further adaptation of avian H5N1...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2013.07.017
更新日期:2013-12-05 00:00:00
abstract::Northern blot analysis revealed the presence of varicella-zoster virus (VZV) gene 27 transcripts in infected cells. The Sal I-G DNA fragment, located in the unique long segment of the VZV genome and containing overlapping genes 26 and 27, was analyzed in an in vitro transcription-translation system. Translation of RNA...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(88)90015-9
更新日期:1988-05-01 00:00:00
abstract::Fourteen isolates of bovine herpesvirus 1 (BHV-1) found representative of more than 100 isolates studied, were compared by restriction fragment pattern analyses and molecularly characterized. A number of evolutionary links between the variants originally associated with infectious bovine rhinotracheitis and the varian...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(96)01383-4
更新日期:1996-12-01 00:00:00
abstract::Echovirus 18 (EV18) is one of the echovirus serotypes associated with human diseases and in particular aseptic meningitis. To facilitate studies of the molecular epidemiology of EV18 and the evolution of enteroviruses in general, the complete nucleotide (nt) sequence was determined for the echovirus 18 prototype strai...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(02)00019-9
更新日期:2002-04-23 00:00:00
abstract::Severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) is recognized as one of the life-threatening viruses causing the most destructive pandemic in this century. The genesis of this virus is still unknown. To elucidate its molecular evolution and regulation of gene expression, the knowledge of codon usage is a...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2020.198113
更新日期:2020-10-15 00:00:00
abstract::Comprehensive pathogenesis studies on Peste des Petits Ruminants virus (PPRV) have been delayed so far by the absence of a small animal model reproducing the disease or an in vitro biological system revealing virulence differences. In this study, a mouse 10T1/2 cell line has been identified as presenting different sus...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2020.198035
更新日期:2020-09-01 00:00:00
abstract::Viruses are strict intracellular pathogens that require the cellular environment to complete a successful infection. Among them, African swine fever virus (ASFV) is an evolutionary ancient DNA virus, endemic in Africa, which is nowadays causing an emergent disease in Europe with a potential high economic impact in the...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2015.05.026
更新日期:2015-11-02 00:00:00
abstract::Hepatitis B virus (HBV) surface antigen (HBsAg), which is encoded by the HBV S gene, is conventionally classified into 4 serological subtypes, adw, adr, ayw and ayr. To determine the relationship between the HBsAg seroreactivity and the nucleotide sequence diversity of the HBV S gene, the nucleotide sequences of S gen...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(95)00069-0
更新日期:1995-11-01 00:00:00
abstract::The inner capsid protein VP6 of group A rotavirus possesses group and subgroup epitope specificities. Avian rotaviruses have a unique VP6 that is antigenically different from its mammalian counterpart. The lack of information on the VP6 protein of chicken rotavirus strain, CH2, at the genetic and antigenic level was a...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2008.07.008
更新日期:2008-11-01 00:00:00
abstract::Viral replication and neutralization of hepatitis C viruses (HCV) have been studied using the infectious molecular clone JFH-1. By passaging JFH-1 in hepatoma cells in the absence or presence of HCV neutralizing antibodies (nAbs), we investigated the molecular mechanisms of cell-culture adaptation and sensitivity to n...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.07.022
更新日期:2012-10-01 00:00:00