Abstract：
:Echovirus 18 (EV18) is one of the echovirus serotypes associated with human diseases and in particular aseptic meningitis. To facilitate studies of the molecular epidemiology of EV18 and the evolution of enteroviruses in general, the complete nucleotide (nt) sequence was determined for the echovirus 18 prototype strain (Metcalf, EV18M). Excluding the poly A sequence, the genome consists of 7410 nt divided into a 740 nt 5' untranslated region (5' UTR), a 6567 nt long open reading frame coding for a 2189 amino acid (aa) polyprotein and a 103 nt 3' UTR. Molecular analysis of the EV18M genome showed a typical enterovirus-like organization. Phylogenetic analysis of the structural and non-structural genes revealed a pattern of different relationships to other echo- and coxsackieviruses. Similarity analysis demonstrated that the Hill strain of echovirus 9 is most likely the result of a previous recombination event between ancestors of the echovirus 9 strain Barty (5' half of the genome) and EV18M (3' half). Using a maximum likelihood approach, the recombination point was mapped to the 2C gene.
journal_name
Virus Resjournal_title
Virus researchauthors
Andersson P,Edman K,Lindberg AMdoi
10.1016/s0168-1702(02)00019-9subject
Has Abstractpub_date
2002-04-23 00:00:00pages
71-83issue
1eissn
0168-1702issn
1872-7492pii
S0168170202000199journal_volume
85pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract：:Coronavirus genome replication is mediated by a multi-subunit protein complex that is comprised of more than a dozen virally encoded and several cellular proteins. Interactions of the viral replicase complex with cis-acting RNA elements located in the 5' and 3'-terminal genome regions ensure the specific replication o...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2014.10.001
更新日期：2014-12-19 00:00:00
abstract：:The epitopes of six monoclonal antibodies generated against type A12 foot-and-mouth disease virus (FMDV) VP1 or its largest cyanogen bromide fragment (13 kd) were characterized. Five of these monoclonal antibodies neutralized viral infectivity. Solid-phase and competitive antigen binding assays using virion-derived an...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(84)90006-6
更新日期：1984-09-01 00:00:00
abstract：:The immune response to a naked plasmid DNA encoding the nucleoprotein (N protein) of porcine transmissible gastroenteritis virus (TGEV) was investigated in this study. A complementary DNA of the entire N gene was amplified by RT-PCR, and inserted into a mammalian expression vector (pcDNA3.1) to construct a recombinant...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(01)00333-1
更新日期：2001-11-28 00:00:00
abstract：:Tobacco (cv. Xanthi nn) plants were watered with allopurinol [4-hydroxypyrazolo (3,4-d) pyrimidine, HPP], a xanthine oxidoreductase (XOR) inhibitor, to investigate its effects on infection by Tobacco mosaic virus engineered to express the green fluorescent protein (TMV.GFP). TMV.GFP infection was monitored by examinat...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.05.018
更新日期：2008-11-01 00:00:00
abstract：:Central nervous system (CNS) infection with herpes simplex virus (HSV)-1 triggers neuroinflammatory responses leading to peripheral immune cell infiltration into the brain. Previous in vitro studies from our laboratory, using primary human brain cells, implicated microglia as the cellular source of infection-induced c...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2006.03.009
更新日期：2006-10-01 00:00:00
abstract：:The positive sense genomes of members of the genus Flavivirus in the family Flaviviridae are ∼ 11 kb in length and have a 5' type I cap but no 3' poly-A. The 3' and 5' terminal regions contain short conserved sequences that are proposed to be repeated remnants of an ancient sequence. However, the functions of most of ...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2015.02.006
更新日期：2015-08-03 00:00:00
abstract：:Phylogenetic analysis has been extensively used as a tool for the reconstruction of epidemiological relations for research or for forensic purposes. It was our objective to assess the sensitivity of different phylogenetic methods and various phylogenetic programs to reconstruct epidemiological links among HIV-1 infect...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.03.002
更新日期：2012-06-01 00:00:00
abstract：:The nucleotide sequences of the nucleoprotein (NP) genes of fowl plague virus (FPV) and of a temperature-sensitive (ts) mutant (ts81) derived therefrom have been determined. The ts81-NP nucleotide sequence possesses a single nucleotide substitution in comparison to the wild type. This causes an amino acid exchange at ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90058-0
更新日期：1989-02-01 00:00:00
abstract：:The variability of the Apple stem pitting virus (ASPV) coat protein (CP) gene was investigated. The CP gene of ten virus isolates from apple and pear trees was sequenced. Comparison of all sequenced virus isolates revealed high diversity of the CP gene (70.7-93.5% at the nucleotide level and 77.8-98.7% at the amino ac...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2011.03.003
更新日期：2011-06-01 00:00:00
abstract：:Enterovirus 71 (EV71) is a single-stranded RNA virus that belongs to Picornaviridae family. It causes the hand-foot-and-mouth disease and fatal neurological diseases in young children and infants. The mechanism of EV71 pathogenesis remains obscure. The intestinal tract is the initial site of EV71 replication, but no o...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.05.002
更新日期：2013-09-01 00:00:00
abstract：:An insect High-Five cell line was generated constitutively and stably expressing the VP2 protein of rotavirus RF strain leading to the formation of core-like particles. The integration of the VP2 gene was confirmed by PCR, Real-time PCR and Southern blot, and the protein expression was confirmed by Western blot and im...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.12.015
更新日期：2013-03-01 00:00:00
abstract：:We have determined the post-translational modifications of the major capsid protein, L1 of human papillomavirus (HPV) type 6b. Since this virus cannot be cultured in the laboratory to obtain sufficient material for a study, a recombinant L1 protein produced in a vaccinia virus expression system was used in this invest...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(98)00117-8
更新日期：1999-04-01 00:00:00
abstract：:Infectious salmon anaemia virus (ISAV) is an orthomyxovirus causing anaemia and circulatory disease with high mortality in farmed Atlantic salmon (Salmo salar). Orthomyxoviruses are unusual as RNA viruses as they replicate in the nucleus and some viral transcripts undergo splicing. The nuclear replication necessitates...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.07.001
更新日期：2013-10-01 00:00:00
abstract：:DNA clones representing the small genomic segment of Nephropathia epidemica virus strain Hällnäs B1 (NEV) and Hantaan virus strain 76-118 (HTV) encoding their nucleocapsid proteins were inserted into the E. coli vector pIN-III-ompA for secretion of proteins into the periplasmic space. The complete HTV and NEV nucleoca...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(91)90090-i
更新日期：1991-03-01 00:00:00
abstract：:Hepatitis B virus (HBV) surface antigen (HBsAg), which is encoded by the HBV S gene, is conventionally classified into 4 serological subtypes, adw, adr, ayw and ayr. To determine the relationship between the HBsAg seroreactivity and the nucleotide sequence diversity of the HBV S gene, the nucleotide sequences of S gen...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(95)00069-0
更新日期：1995-11-01 00:00:00
abstract：:The identification of ligand-binding sites is often the first step in drug targeting and design. To date there are numerous computational tools available to predict ligand binding sites. These tools can guide or mitigate the need for experimental methods to identify binding sites, which often require significant resou...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.05.029
更新日期：2016-08-15 00:00:00
abstract：:Bovine enteroviruses, which belong to the Picornaviridae family, can cause clinical symptoms in cattle and are excreted in feces. In this study, a cytolytic virus was isolated from Madin-Darby bovine kidney (MDBK) cells from fecal samples of bovine with severe diarrhea and hemorrhagic intestinal mucosa that had been o...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.07.032
更新日期：2014-10-13 00:00:00
abstract：:The nucleoprotein (N), phosphoprotein (P) and glycoprotein (G) genes of Irkut and West Caucasian bat viruses (WCBV) were sequenced and compared with those of other lyssaviruses. N gene nucleotide identities provided unequivocal separation of all lyssavirus genotypes with an identity threshold of 82%. On this basis, Ir...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2005.03.008
更新日期：2005-07-01 00:00:00
abstract：:The pathogenesis of chorioallantoic membrane (CAM) infection with herpes simplex virus 1 and 2 (HSV-1 and HSV-2) as well as chick embryo fibroblast (CEF) passaged HSV-1 was studied. It was found that HSV-2 is at least a million-fold more virulent than HSV-1 as measured by pfu/LD50 ratios for the embryo. Serial passage...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(86)90017-1
更新日期：1986-08-01 00:00:00
abstract：:The disulphide-linked GP2/3/4 spike of equine arteritis virus (EAV) is essential for virus entry. We showed recently that in transfected cells carbohydrates attached adjacent to the signal peptide of GP3 inhibit cleavage. Here we confirm this unique phenomenon in recombinant viruses with disabled glycosylation sites. ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.02.005
更新日期：2014-04-01 00:00:00
abstract：:Human herpesvirus 6 (HHV-6) is now classified into two distinct variants such as HHV-6 variant A(HHV-6A) and B(HHV-6B) (Ablashi et al., Arch. Virol. 129, 1993, 1-4) and the DNA of HHV-6A strain U1102 was completely sequenced (Gompels et al., Virology 209, 1995, 29-51). We have sequenced a 30-kilobase pair (kbp) (genom...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(97)00099-3
更新日期：1997-11-01 00:00:00
abstract：:A flow cytometric assay that measures binding of human T-lymphotropic virus type 1 (HTLV-1) virions to target cells was used to investigate the binding process and to screen for compounds affecting viral binding. Results showed that adenosine receptor type 2 antagonists effectively inhibit viral binding at concentrati...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(03)00048-0
更新日期：2003-05-01 00:00:00
abstract：:Currently, pseudorabies virus (PRV) variant strains are outbreaking in China; these variants belong to genotype II PRV. The traditional Bartha-K61 vaccine has failed to provide complete protection against the emergent variant strains. Therefore, rapid attenuation of current epidemic strains is needed for effective PRV...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.09.004
更新日期：2016-10-02 00:00:00
abstract：:In recent years, the spread of Duck Hepatitis A Viruses (DHAVs) has represented a serious threat and significant economic impact in duck industry of China. The sixteen reported DHAV isolates (15 DHAV-1 strains and one DHAV-3) were identified from infected ducks with clinical symptoms in China between 2009 and 2012. In...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.10.007
更新日期：2013-12-26 00:00:00
abstract：:The subcellular distribution of influenza polymerase PA subunit has been studied using a SV40-recombinant virus (SVPA76), which allows the expression and accumulation of this protein in COS-1 cells. In contrast to the complete nuclear localization observed for the PA subunit several hours after influenza virus infecti...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(92)90031-4
更新日期：1992-06-01 00:00:00
abstract：:We have investigated the infection of Aedes albopictus (mosquito) cell clones by Sindbis virus. Variation in the multiplicity of infection (MOI) from ranges of 50-0.00005 pfu/cell was determined to have no effect on the progression of the infection to high acute phase titer, suggesting that intracellular factors alone...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(97)01461-5
更新日期：1997-07-01 00:00:00
abstract：:The ultrastructure of the equine morbillivirus (EMV) which was implicated in the death of one human and fourteen horses in Queensland, Australia during September 1994 and a 36 year old man from Queensland in October 1995 is described. The ultrastructure of the virus and the intracellular virus-specific structures are ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(96)01307-x
更新日期：1996-07-01 00:00:00
abstract：:Two genetic markers--the thymidine kinase gene of herpes simplex virus, and the beta-galactosidase gene of Escherichia coli--were incorporated into the 36K protein gene (IL1 gene according to the nomenclature of the Copenhagen strain of vaccinia virus; Goebel et al., 1990) from the HindIII-P DNA fragment of the LIVP s...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(93)90027-k
更新日期：1993-06-01 00:00:00
abstract：:There are over 350 million people chronically infected with the Hepatitis B virus (HBV); chronic HBV infections are associated with the development of hepatocellular carcinoma (HCC). While the precise mechanism of HBV-associated HCC remains undefined, it is believed to involve a combination of the host immune response...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2010.09.023
更新日期：2011-01-01 00:00:00
abstract：:A disease of Rudbeckia hirta (Black-eyed Susan), characterized by severe flower deformation, was observed in Minnesota during 2010-2016. A previously undescribed virus species, named Rudbeckia flower distortion virus (RuFDV, family Caulimoviridae, genus unassigned), was determined to be the causal agent of the disease...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2017.05.012
更新日期：2017-09-15 00:00:00
