Abstract:
:The core proteins of epizootic haemorrhagic disease virus (EHDV) have important roles to perform in maintaining the structure and function of the virus. A complete genetic and phylogenetic analysis was therefore performed on these proteins (and the genes that code for them) to allow comparison of the selective pressures acting on each. Accession numbers, gene and protein sizes, ORF positions, G+C contents, terminal hexanucleotides, start and stop codons and phylogenetic relationships are all presented. The inner core proteins (VP1, VP3, VP4 and VP6) were characterised by high levels of sequence conservation, and the ability to topotype isolates very strongly into eastern or western groups. This is particularly evident in genome segment 9 (VP6) which exists as two different sized homologues. VP7 did not topotype, but rather exhibited a more random, radial phylogeny suggestive of genetic drift. With the exception of VP6, all of the core proteins also showed high numbers of synonymous mutations in the third base position, suggesting they have been evolving for a long period of time. Interestingly, VP6 did not show this, and possible reasons for this are discussed.
journal_name
Virus Resjournal_title
Virus researchauthors
Anthony SJ,Maan N,Maan S,Sutton G,Attoui H,Mertens PPdoi
10.1016/j.virusres.2009.07.011subject
Has Abstractpub_date
2009-11-01 00:00:00pages
187-99issue
2eissn
0168-1702issn
1872-7492pii
S0168-1702(09)00246-9journal_volume
145pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract::A cloned cDNA (1.65 kb) containing the complete glycoprotein gene of the Josiah strain of Lassa virus was inserted into the thymidine kinase (TK) gene of the New York Board of Health (WYETH) strain of vaccinia virus. The Lassa virus glycoprotein precursor, GPC, and the posttranslational cleavage products, G1 and G2, w...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(88)90033-0
更新日期:1988-02-01 00:00:00
abstract::Forty Chinese PCV2 strains collected between 2004 and 2008 were sequenced and their genetic variations were analyzed together with nine previous PCV2 isolates. Phylogenetic analysis indicated that these Chinese PCV2 strains could be divided into four genotypes (PCV-2a, PCV-2b, PCV-2d and PCV-2e), and the genotype PCV-...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2009.05.015
更新日期:2009-10-01 00:00:00
abstract::Hantaviruses are the etiologic agents of Hemorrhagic Fever with Renal Syndrome (HFRS) in Old World, and Hantavirus Pulmonary Syndrome (HPS)/Hantavirus Cardiopulmonary Syndrome (HCPS), in the New World. Serological methods are the most common approach used for laboratory diagnosis of HCPS, however theses methods do not...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2015.07.022
更新日期:2015-12-02 00:00:00
abstract::The mechanism by which hepatitis B virus (HBV) infection causes severe inflammatory liver diseases is multifactorial and related to interactions with cell signaling pathways and the ensuing inflammatory response. Activation of JAK/STAT/SOCS signaling is essential for the induction of cellular antiviral responses, cont...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2009.12.003
更新日期:2010-03-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) replication and transcription activator (RTA) is necessary and sufficient for the switch from KSHV latency to lytic replication. RTA activates promoters by several mechanisms. RTA can bind to sequences in viral promoters and activate transcription. In addition, RTA intera...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2007.07.019
更新日期:2008-01-01 00:00:00
abstract::Fifteen stable mouse spleen cell myeloma hybrids (hybridomas) producing monoclonal antibodies to rinderpest virus proteins were produced. The specificity of these monoclonal antibodies was established by radioimmunoprecipitation followed by polyacrylamide gel analysis and immunofluorescence. Nine antibodies were speci...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(89)90091-9
更新日期:1989-04-01 00:00:00
abstract::A series of recombinant fusion proteins derived from equine arteritis virus (EAV) open reading frame (ORF) 7 have been used to define the immunoreactive region of the viral nucleocapsid (N) protein. Reactivities of recombinant N fusion proteins with post-infection equine sera in immunoblots and ELISAs indicate that th...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(95)00098-4
更新日期:1995-12-01 00:00:00
abstract::Quasispecies dynamics mediates adaptability of RNA viruses through a number of mechanisms reviewed in the present article, with emphasis on the medical implications for the hepatitis viruses. We discuss replicative and non-replicative molecular mechanisms of genome variation, modulating effects of mutant spectra, and ...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2007.02.001
更新日期:2007-08-01 00:00:00
abstract::Viruses and virus-like particles (VLPs) of insect parasitoids modify host-parasite interactions. The Drosophila wasp, Leptopilina heterotoma, produce 300 nm spiked VLPs that bind to the host's blood cells via surface projections. L. heterotoma is a generalist wasp that attacks over a dozen Drosophila species. Oviposit...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2011.06.005
更新日期:2011-09-01 00:00:00
abstract::This study reports the detection by RT-PCR and molecular characterization of partial RdRp gene of picobirnavirus (PBV) dsRNA in fecal samples (n = 100) from a meat sheep flock in southern Brazil. The analysis of the results allowed the identification of two important characteristics of PBV infection. The first was the...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2018.06.016
更新日期:2018-08-15 00:00:00
abstract::Murine cytomegalovirus (MCMV) m137 null mutants, Deltam137A and Deltam137B, were generated by inserting a gpt cassette into a deleted region of the open reading frame. A polyclonal antiserum produced to an Escherichia coli expressed gst-m137 fusion protein was used to show that a 38 kDa polypeptide corresponding to th...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(02)00024-2
更新日期:2002-03-20 00:00:00
abstract::We previously prepared neutralizing monoclonal antibody (MAb)-resistant (mar) mutant viruses using a laboratory strain feline infectious peritonitis virus (FIPV) 79-1146 (Kida et al., 1999). Mar mutant viruses are mutated several amino acids of the neutralizing epitope of Spike protein, compared with the parent strain...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2010.12.020
更新日期:2011-03-01 00:00:00
abstract::Here we document that the gene VI product of peanut chlorotic streak virus (PClSV), a newly characterized member of the group, transactivates the translation of dicistronic transcripts. Dicistronic expression units have been analyzed both in protoplast transient expression experiments and in transgenic tobacco plants....
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(98)00088-4
更新日期:1998-10-01 00:00:00
abstract::The extensive nucleotide sequence heterogeneity among independent genotypes of wild polioviruses permits the systematic design of genotype-specific molecular reagents. We have prepared two sets of polymerase chain reaction (PCR) primer pairs specific for the genotype of wild poliovirus type 3 recently endemic to Mexic...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(92)90124-r
更新日期:1992-08-01 00:00:00
abstract::The resurgence of Chikungunya virus (CHIKV) in the form of unprecedented explosive epidemic after a gap of 32 years in India is a point of major public health concern. In 2007 again there was outbreak in Kerala, India, affecting more than 25,000 cases with many reported mortalities. To understand the molecular basis o...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2008.02.004
更新日期:2008-07-01 00:00:00
abstract::Acute hemorrhagic conjunctivitis (AHC) is a self-limiting viral infection of the eyes but having epidemic potential. In winter 2004-2005, an outbreak of acute hemorrhagic conjunctivitis (AHC) occurred in Islamabad, Pakistan. The etiological agent was confirmed as coxsackievirus A24 variant (CA24v) by virus isolation a...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2008.06.011
更新日期:2008-10-01 00:00:00
abstract::Coronaviruses are responsible for a growing economic, social and mortality burden, as the causative agent of diseases such as severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), avian infectious bronchitis virus (IBV) and COVID-19. However, there is a lack of effective antiviral agents f...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2020.197989
更新日期:2020-07-15 00:00:00
abstract::Nucleic acid probes prepared directly from bluetongue virus (BTV) genomic double-stranded RNA (dsRNA) have been used to identify the functionally equivalent genome segments from six distinct isolates of BTV after their separation in both agarose and polyacrylamide gel electrophoresis systems. Variations in the rate, a...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(88)90078-0
更新日期:1988-06-01 00:00:00
abstract::Our understanding of picornavirus RNA replication has improved over the past 10 years, due in large part to the discovery of cis-active RNA elements (CREs) within picornavirus RNA genomes. CREs function as templates for the conversion of VPg, the Viral Protein of the genome, into VPgpUpU(OH). These so called CREs are ...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2008.07.027
更新日期:2009-02-01 00:00:00
abstract::Subtype C is the most prevalent HIV-1 subtype globally and a common circulating recombinant form virus, CRF_BC, is predominant in China. It is unclear whether subtype C-based vaccines are capable of neutralizing CRF_BC viruses or can be evaluated using a neutralization assay based on CRF_BC pseudoviruses. Phylogenetic...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2010.09.012
更新日期:2011-01-01 00:00:00
abstract::Three dendrimeric peptides were synthesized in order to evaluate their immunogenicity and their potential protection against classical swine fever virus (CSFV) in domestic pigs. Construct 1, an optimized version of a previously used dendrimer, had four copies of a B-cell epitope derived from CSFV E2 glycoprotein conne...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2017.05.020
更新日期:2017-06-15 00:00:00
abstract::The genomes of eight hepatitis A virus (HAV) strains originating from far distant geographic regions such as Europe, North Africa, Middle and North America, Australia and The People's Republic of China were compared by RNase T1 oligonucleotide mapping. For this purpose, the viruses were propagated in cell cultures and...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(85)90020-6
更新日期:1985-12-01 00:00:00
abstract::Porcine deltacoronavirus (PDCoV), a newly discovered enteric coronavirus, is a causative agent of severe clinical diarrhea and intestinal pathological damage in piglets. As a first step toward understanding the effect of PDCoV on host cells, we elucidated mechanisms underlying the process of apoptotic cell death after...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2018.06.008
更新日期:2018-07-15 00:00:00
abstract::Infection by the human immunodeficiency virus type 1 (HIV-1) is initiated through interaction of its exterior envelope glycoprotein gp120 with the CD4 receptor on target cells. To address the possible role of N-glycosylation of HIV-1 gp120 in binding CD4, we mutated different conserved N-glycosylation site Asn-residue...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(90)90085-p
更新日期:1990-12-01 00:00:00
abstract::Orf virus infection causes a contagious pustular dermatitis characterized by extensive vascular changes that have been linked to a virally encoded vascular endothelial growth factor (VEGF). The VEGF genes of different strains of orf virus can vary extensively in amino acid sequence. Functional analyses of two major va...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2007.04.018
更新日期:2007-09-01 00:00:00
abstract::Certain insect cell lines have been shown to be permissive (TN-368) or semipermissive (IPLB-LD-652Y) for Autographa californica nuclear polyhedrosis virus (AcMNPV) replication (McClintock et al., 1986b). In this report DNA-binding proteins were identified in such cell:virus systems by hybridizing Western blots contain...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(91)90105-5
更新日期:1991-07-01 00:00:00
abstract:BACKGROUND:HTLV-2 infections are almost always asymptomatic, and diseases associated with the infection are rarely reported. Little information is available on the relationship between HTLV-2 proviral load and gender or expression of disease, especially among patients with HIV-1 co-infection. METHODS:We studied 77 HTL...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2008.01.015
更新日期:2008-07-01 00:00:00
abstract::Post-translational modifications (PTMs) are the chemical modifications of proteins after translation, and are very important to guarantee the proper biological functions of these proteins. Baculoviruses are pathogenic viruses that infect invertebrates and have large circular double-stranded DNA genomes. Many proteins ...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2020.197865
更新日期:2020-04-02 00:00:00
abstract::Okra leaf curl disease (OLCD) is the most important viral disease of okra in West Africa. In this study, a complex of begomoviruses and associated DNA satellites were identified in symptomatic okra plants from southwestern Cameroon. Sequence analyses showed that two of the plants (Lik1 and Njo5) were infected with a b...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2013.03.010
更新日期:2013-06-01 00:00:00
abstract::Typically plasma viremia is controlled in macaques challenged with R5 tropic SHIV162P3 during the chronic phase of infection. However, in a few infected animals it sustains for a prolonged period. Persistent plasma viremia in two such macaques led to a rapid decline of CD4+ T-cells in blood leading to the induction of...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2010.04.006
更新日期:2010-08-01 00:00:00