Abstract：
:Certain insect cell lines have been shown to be permissive (TN-368) or semipermissive (IPLB-LD-652Y) for Autographa californica nuclear polyhedrosis virus (AcMNPV) replication (McClintock et al., 1986b). In this report DNA-binding proteins were identified in such cell:virus systems by hybridizing Western blots containing uninfected and infected cell proteins with AcMNPV or host DNA probes. In the AcMNPV-infected TN-368 permissive cell system, 8 virus-induced DNA-binding proteins with molecular weights ranging from 67.5K to 18.75K were observed under the highest conditions of stringency. When these DNA-binding proteins were compared to structural proteins of AcMNPV, several appeared to be similar to those observed in SDS-PAGE protein profiles of nonoccluded virus (NOV) and occlusion body (PIBs) preparations. Using an AcMNPV occlusion negative mutant (L1GP-gal3) and an anti-AcMNPV-polyhedrin monoclonal antibody, a major DNA-binding protein (33.0K), observed in the permissive system, corresponded to polyhedrin and to a comigrating virus-induced DNA-binding protein. In the AcMNPV-infected IPLB-LD-652Y semipermissive cell system, no virus-induced DNA-binding proteins were detected. However, several host DNA-binding proteins were present but their ability to bind DNA decreased significantly following infection.
journal_name
Virus Resjournal_title
Virus researchauthors
McClintock JT,Guzo D,Guthrie KP,Dougherty EMdoi
10.1016/0168-1702(91)90105-5subject
Has Abstractpub_date
1991-07-01 00:00:00pages
133-45issue
2eissn
0168-1702issn
1872-7492pii
0168-1702(91)90105-5journal_volume
20pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract：:We recently described a giant double stranded DNA virus called Mimivirus, isolated from amoebae, which might represent a new pneumonia-associated human pathogen. Its unique morphological and genomic characteristics allowed us to propose Mimivirus as a member of a new distinct Nucleocytoplasmic Large DNA viruses family...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2005.07.011
更新日期：2006-04-01 00:00:00
abstract：:The entry of herpes simplex virus (HSV) into an oral epithelial cell line, primary normal human oral keratinocytes (NHOK) and gingival fibroblasts (GF) was examined. Infection of these cells by HSV-1 and HSV-2 was blocked by heparin. Further examination indicated that heparin reduced viral attachment but not penetrati...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(02)00055-2
更新日期：2002-06-01 00:00:00
abstract：:JCPyV and BKPyV are common human polyomaviruses that cause lifelong asymptomatic persistent infections in their hosts. In immunosuppressed individuals, increased replication of JCPyV and BKPyV cause significant disease. JCPyV causes a fatal and rapidly progressing demyelinating disease known as progressive multifocal ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.06.008
更新日期：2014-08-30 00:00:00
abstract：:The human respiratory coronavirus OC43 was grown on a human rectal tumor cell line and was isotopically labeled with amino acids, glucosamine, and orthophosphate to analyze virion structural proteins. Four major protein species were resolved by electrophoresis and many of their properties were deduced from digestion s...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(86)90013-4
更新日期：1986-08-01 00:00:00
abstract：:The varicella zoster virus (VZV) glycoprotein H (gH) stimulates VZV-specific immune responses and may be involved in virus penetration. This study reports the genomic map position and the DNA sequence of a simian varicella virus (SVV) homologue of the VZV gH gene. A 32P-labeled VZV gH-specific DNA probe hybridized to ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(95)00049-v
更新日期：1995-09-01 00:00:00
abstract：:We performed phylogenetic and cluster analysis of human rhinovirus species A (HRV-A) isolated from 76 children with acute respiratory infection in Yamagata prefecture, Japan during the period 2003-2007. Phylogenetic trees based on the nucleotide and amino acid sequences of the VP4/VP2 coding region showed that the pre...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.11.011
更新日期：2010-02-01 00:00:00
abstract：:To investigate the evolutionary origins of proteins encoded by the Poxviridae family of viruses, we examined all poxvirus protein coding genes using a method of characterizing and visualizing the similarity between these proteins and taxonomic subsets of proteins in GenBank. Our analysis divides poxvirus proteins into...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.05.006
更新日期：2009-09-01 00:00:00
abstract：:Our understanding of picornavirus RNA replication has improved over the past 10 years, due in large part to the discovery of cis-active RNA elements (CREs) within picornavirus RNA genomes. CREs function as templates for the conversion of VPg, the Viral Protein of the genome, into VPgpUpU(OH). These so called CREs are ...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2008.07.027
更新日期：2009-02-01 00:00:00
abstract：:Borna disease virus grows to low titres in persistently infected cells with an infectious particle to cell ratio of 0.01 to 0.05. Inclusion of n-butyrate in the growth medium enhances infectivity yields up to 1 log. This effect is time and concentration dependent. In hypertonic medium with an excess of NaCl, KCl or Na...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(85)90057-7
更新日期：1985-02-01 00:00:00
abstract：:Canine astrovirus RNA was detected in the stools of 17/63 (26.9%) samples, using either a broadly reactive consensus RT-PCR for astroviruses or random RT-PCR coupled with massive deep sequencing. The complete or nearly complete genome sequence of five canine astroviruses was reconstructed that allowed mapping the geno...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.12.005
更新日期：2017-03-15 00:00:00
abstract：:Since 2002 there has been a rise in arthrogryposis/hydranencephaly (AGH) incidence in Israel, caused by Akabane (AKA) and, possibly, Aino viruses. To test the ability to control the disease, three siRNA genes targeted to the S genome segment were designed and prepared in the form of siRNA cassettes. For the design all...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2006.02.009
更新日期：2006-09-01 00:00:00
abstract：:Fruit trees of temperate and tropical climates are of great economical importance worldwide and several viruses have been reported affecting their productivity and longevity. Fruit trees of different Brazilian regions displaying virus-like symptoms were evaluated for infection by circular DNA viruses. Seventy-four fru...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2015.07.005
更新日期：2015-12-02 00:00:00
abstract：:The core proteins of epizootic haemorrhagic disease virus (EHDV) have important roles to perform in maintaining the structure and function of the virus. A complete genetic and phylogenetic analysis was therefore performed on these proteins (and the genes that code for them) to allow comparison of the selective pressur...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.07.011
更新日期：2009-11-01 00:00:00
abstract：:Viruses of the genus 'Norwalk-like viruses' (NLVs) detected in humans have been genetically classified into two major genetic groups, genogroups I and II (GI and GII), which together are made up of at least 14 genetic subgroups. However, a comparable classification of NLVs in other species remains to be carried out. W...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(02)00107-7
更新日期：2002-08-01 00:00:00
abstract：:Quasispecies dynamics mediates adaptability of RNA viruses through a number of mechanisms reviewed in the present article, with emphasis on the medical implications for the hepatitis viruses. We discuss replicative and non-replicative molecular mechanisms of genome variation, modulating effects of mutant spectra, and ...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2007.02.001
更新日期：2007-08-01 00:00:00
abstract：:In the past few decades, dengue has spread rapidly and is an emerging disease in China. An unexpected dengue outbreak occurred in Xishuangbanna, Yunnan, China, resulting in 1331 patients in 2013. In order to obtain the complete genome information and perform mutation and evolutionary analysis of causative agent relate...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2017.06.015
更新日期：2017-06-15 00:00:00
abstract：:We here report the results of an investigation of the effect of interferon on the establishment of new infections by a retrovirus. For this study, we used an infectious but replication-incompetent retrovirus carrying a drug-resistance gene and assayed for infectivity by measuring drug-resistant colony formation. Mouse...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90075-0
更新日期：1989-08-01 00:00:00
abstract：:The mammalian reoviruses possess a genome composed of 10 double stranded RNA segments. The serotype 1 strain Lang M1 segment was sequenced and compared to the published type 3 sequence. Both segments were 2304 base-pairs long coding for the mu 2 protein predicted to be 736 amino acids long. The sequences were highly c...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(92)90042-8
更新日期：1992-02-01 00:00:00
abstract：:As master transcription factors for lipogenesis, sterol regulatory element-binding protein-1 (SREBP-1) has two isoforms, SREBP-1a and SREBP-1c. Hepatitis B virus X (HBx) can up-regulate the transcription of both SREBP-1a and SREBP-1c. HBx is a small protein consisting of 154 amino acids. Truncated forms of HBx, often ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2015.07.011
更新日期：2015-12-02 00:00:00
abstract：BACKGROUND:HTLV-2 infections are almost always asymptomatic, and diseases associated with the infection are rarely reported. Little information is available on the relationship between HTLV-2 proviral load and gender or expression of disease, especially among patients with HIV-1 co-infection. METHODS:We studied 77 HTL...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.01.015
更新日期：2008-07-01 00:00:00
abstract：:A simple and sensitive method for the detection of measles virus genome was developed, amplifying the regions encoding the nucleocapsid (N) protein and hemagglutinin (H) protein of measles virus by reverse transcriptase-polymerase chain reaction (RT-PCR). We examined a variety of measles patients: 28 patients with nat...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(94)00074-m
更新日期：1995-01-01 00:00:00
abstract：:Retroviruses and retrotransposons package genomic RNA into virus-like particles (VLPs) in a poorly understood process. Expression of the budding yeast retrotransposon Ty3 results in the formation of cytoplasmic Ty3 VLP assembly foci comprised of Ty3 RNA and proteins, and cellular factors associated with RNA processing...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.10.008
更新日期：2013-02-01 00:00:00
abstract：:Numerous host factors are required for the efficient replication of rotavirus, including the activation and inactivation of several cell signaling pathways. One of the cellular structures that are reorganized during rotavirus infection is the actin cytoskeleton. In this work, we report that the dynamics of the actin m...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2019.01.003
更新日期：2019-04-02 00:00:00
abstract：:Despite the fact that cells infected with wild type vaccinia virus synthesize viral DNA and assemble progeny virus particles within the cytoplasm, the host cell nucleus is required for a productive infection. Recent evidence suggests that vaccinia virus selectively recruits components from the host cell nucleus into t...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/0168-1702(87)90014-1
更新日期：1987-09-01 00:00:00
abstract：:The nucleotide sequences of the nucleoprotein (NP) genes of fowl plague virus (FPV) and of a temperature-sensitive (ts) mutant (ts81) derived therefrom have been determined. The ts81-NP nucleotide sequence possesses a single nucleotide substitution in comparison to the wild type. This causes an amino acid exchange at ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90058-0
更新日期：1989-02-01 00:00:00
abstract：:This study reports the detection by RT-PCR and molecular characterization of partial RdRp gene of picobirnavirus (PBV) dsRNA in fecal samples (n = 100) from a meat sheep flock in southern Brazil. The analysis of the results allowed the identification of two important characteristics of PBV infection. The first was the...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2018.06.016
更新日期：2018-08-15 00:00:00
abstract：:Two lines of LLC-MK2 cells persistently infected with human parainfluenza virus 3 (HPIV-3) have been maintained in culture for approximately 3 years. Subgenomic RNAs (putative defective interfering particle genomes) were detected in virions released from both persistently infected cultures. In one of the persistently ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(90)90039-e
更新日期：1990-04-01 00:00:00
abstract：:In 1995, a new human hepatitis virus belonging to the family Flaviviridae was described and designated hepatitis GBV-C. To investigate variations within the genome of GBV-C and to study the relationship of GBV-C to GBV-A/B or hepatitis C virus (HCV), we established a detection system using reverse transcriptase polyme...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(96)01377-9
更新日期：1996-12-01 00:00:00
abstract：:Influenza A viruses are characterized by a unique genome structure, causing genetic instability, especially to the genes of haemagglutinin and neuraminidase. The objectives of this research was molecular and phylogenetic analysis of influenza A(H3N2) strains that circulated in Northern Greece since 2004, particularly ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.07.006
更新日期：2009-11-01 00:00:00
abstract：:Hepatitis C virus (HCV) is a major cause of global morbidity and mortality, with an estimated 130-150 million infected individuals worldwide. HCV is a leading cause of chronic liver diseases including cirrhosis and hepatocellular carcinoma. Current treatment options in developing countries involve pegylated interferon...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.07.008
更新日期：2016-09-02 00:00:00
