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Sequence analysis of hepatitis GB virus C (GBV-C) isolates from 14 patients.

Abstract：

:In 1995, a new human hepatitis virus belonging to the family Flaviviridae was described and designated hepatitis GBV-C. To investigate variations within the genome of GBV-C and to study the relationship of GBV-C to GBV-A/B or hepatitis C virus (HCV), we established a detection system using reverse transcriptase polymerase chain reaction (RT-PCR) of the putative helicase region (NS3). So far, isolates derived from 14 different GBV-C-positive sera were analyzed (GBV-C/S3-36), showing 80.1-89.4% (mean: 85%) identical nucleotides. The deduced amino acid sequences revealed 97.3% homology. Nucleotide sequences of GBV-C/S3-36 revealed about 60% identity to GBV-A as well as to HCV, but only 56% identity to GBV-B. Amino acid sequences revealed 73.4 and 68.6% similarity to GBV-A and GBV-B, respectively, but a slightly higher percentage of 78.5% to HCV sequences. Thus, according to the putative GBV-C helicase sequence, a subtyping of GBV-C into different genotypes may be necessary.

journal_name

Virus Res

journal_title

Virus research

authors

Seipp S,Wahl R,Mueller H,Stremmel W,Theilmann L,Goeser T

doi

10.1016/s0168-1702(96)01377-9

subject

Has Abstract

pub_date

1996-12-01 00:00:00

pages

81-8

issue

1-2

eissn

0168-1702

issn

1872-7492

pii

S0168-1702(96)01377-9

journal_volume

46

pub_type

杂志文章

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