当前位置： SCI文献检索 > VIRUS RESEARCH期刊下所有文献 > HBx truncation mutants differentially modulate SREBP-1a and -1c transcription and HBV replication.

HBx truncation mutants differentially modulate SREBP-1a and -1c transcription and HBV replication.

Abstract：

:As master transcription factors for lipogenesis, sterol regulatory element-binding protein-1 (SREBP-1) has two isoforms, SREBP-1a and SREBP-1c. Hepatitis B virus X (HBx) can up-regulate the transcription of both SREBP-1a and SREBP-1c. HBx is a small protein consisting of 154 amino acids. Truncated forms of HBx, often found in the tissues after HBV infection, may have a role in the pathogenesis associated with HBV infection. In this study, we examined the effects of two HBx truncation mutants, HBx aa. 1-127 and HBx aa. 43-154, on the transcription of SREBP-1a and SREBP-1c. HBx 1-127 can up-regulate SREBP-1c, but not SREBP-1a transcription, whereas HBx 43-154 can activate SREBP-1a, but not SREBP-1c transcription. We further determined the activities of two HBV enhancers after the expression of the truncated HBx proteins. HBx 1-127 and HBx 43-154 can only up-regulate HBV enhancer I or HBV enhancer II, respectively. Knocking down SREBP-1 abrogates enhancer activation by HBx proteins, suggesting a role of SREBP-1. In addition, using HBV enhancer mutants, we found that the binding sequence for AP-1 on enhancer I is essential for its activation by HBx 1-127, whereas C/EBP and Sp1 sites are required for enhancer II activation by HBx 43-154. Finally, we showed that both HBx 1-127 and HBx 43-154 can increase HBV transcription and HBV replication dependent upon SREBP-1 because knocking down SREBP-1 abrogates the up-regulation. Furthermore, upon ectopic expression of either SREBP-1a or SREBP-1c, we showed that SREBP-1a is involved in HBV transcription and replication up-regulation by HBx 43-154, whereas SREBP-1c is involved in HBV transcription and replication up-regulation by HBx 1-127. Our results should help understand the interactions between HBV and the SREBP-1-mediated lipogenic pathway.

journal_name

Virus Res

journal_title

Virus research

authors

Wu Q,Liu Q

doi

10.1016/j.virusres.2015.07.011

subject

Has Abstract

pub_date

2015-12-02 00:00:00

pages

46-53

eissn

0168-1702

issn

1872-7492

pii

S0168-1702(15)30015-0

journal_volume

210

pub_type

杂志文章

相关文献

VIRUS RESEARCH文献大全
  • AIDS pathogenesis: the role of accessory gene mutations, leading to formation of long-lived persistently infected cells and/or apoptosis-inducing HIV-1 particles.

    abstract：:Human immunodeficiency virus type 1 (HIV-1) infection indirectly induces activation-dependent apoptosis in bystander immune CD4+ T-cells, a hallmark of AIDS pathogenesis. It is well known that this pathogenetic event is significantly correlated with a high virus load. Active viral replication occurs in HIV-1 asymptoma...

    journal_title：Virus research

    pub_type： 杂志文章,评审

    doi：10.1016/s0168-1702(97)00125-1

    authors： Ikuta K,Kameoka M,Luftig RB

    更新日期：1997-12-01 00:00:00

  • Plant subviral RNAs as a long noncoding RNA (lncRNA): Analogy with animal lncRNAs in host-virus interactions.

    abstract：:Satellite RNAs (satRNAs) and viroids belong to the group called subviral agents and are the smallest pathogens of plants. In general, small satRNAs and viroids are 300-400 nt in size and do not encode any functional proteins; they are thus regarded as so-called long noncoding RNAs (lncRNAs). These lncRNAs are receivin...

    journal_title：Virus research

    pub_type： 杂志文章,评审

    doi：10.1016/j.virusres.2015.06.016

    authors： Shimura H,Masuta C

    更新日期：2016-01-02 00:00:00

  • NF-κB is required for dengue virus NS5-induced RANTES expression.

    abstract：:Dengue virus (DENV) infection associates with renal disorders. Patients with dengue hemorrhagic fever and acute kidney injury have a high mortality rate. Increased levels of cytokines may contribute to the pathogenesis of DENV-induced kidney injury. Currently, molecular mechanisms how DENV induces kidney cell injury h...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2014.12.007

    authors： Khunchai S,Junking M,Suttitheptumrong A,Kooptiwut S,Haegeman G,Limjindaporn T,Yenchitsomanus PT

    更新日期：2015-02-02 00:00:00

  • Effect of reaction conditions and 3AB on the mutation rate of poliovirus RNA-dependent RNA polymerase in a alpha-complementation assay.

    abstract：:The fidelity of poliovirus RNA-dependent RNA polymerase (3Dpol) was determined using a modified alpha-complementation assay. Several polymerases have been analyzed by this approach allowing comparisons to be drawn. Various conditions including high and low MgCl(2), replacing MgCl(2) with MnCl(2), skewed nucleotide poo...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2009.10.006

    authors： DeStefano JJ

    更新日期：2010-01-01 00:00:00

  • Live attenuated pseudorabies virus developed using the CRISPR/Cas9 system.

    abstract：:Currently, pseudorabies virus (PRV) variant strains are outbreaking in China; these variants belong to genotype II PRV. The traditional Bartha-K61 vaccine has failed to provide complete protection against the emergent variant strains. Therefore, rapid attenuation of current epidemic strains is needed for effective PRV...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2016.09.004

    authors： Tang YD,Liu JT,Wang TY,An TQ,Sun MX,Wang SJ,Fang QQ,Hou LL,Tian ZJ,Cai XH

    更新日期：2016-10-02 00:00:00

  • Identification, sequence analysis and phylogeny of the lef-2 gene of Helicoverpa armigera single-nucleocapsid baculovirus.

    abstract：:The baculovirus late expression factor 2 (LEF-2) is involved in DNA replication, and most likely function as a primase processivity factor. Lef-2 genes have been found in multinucleocapsid nucleopolyhedroviruses (MNPVs) and in granuloviruses (GVs), but not yet in single-nucleocapsid NPV (SNPV). Here, a lef-2 gene homo...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/s0168-1702(99)00097-0

    authors： Chen X,Ijkel WF,Dominy C,de Andrade Zanotto PM,Hashimoto Y,Faktor O,Hayakawa T,Wang CH,Prekumar A,Mathavan S,Krell PJ,Hu Z,Vlak JM

    更新日期：1999-12-01 00:00:00

  • Beta-actin interacts with the E2 protein and is involved in the early replication of classical swine fever virus.

    abstract：:The E2 glycoprotein of classical swine fever virus (CSFV) is involved in viral infection and induction of neutralizing antibodies. Little is known about how E2 interacts with host cells. To understand the cellular factors involved in viral replication cycle, E2 was used as bait in yeast two-hybrid screens, resulting i...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2013.10.016

    authors： He F,Ling L,Liao Y,Li S,Han W,Zhao B,Sun Y,Qiu HJ

    更新日期：2014-01-22 00:00:00

  • The nucleotide sequence and genome organization of the whitefly transmitted sweetpotato mild mottle virus: a close relationship with members of the family Potyviridae.

    abstract：:Primers corresponding to conserved regions in the RNA-dependent RNA polymerase and the RACE procedure led to the cloning of the complete sweetpotato mild mottle virus (SPMMV) RNA genome. The assembled SPMMV genomic sequence was 10,818 nucleotides in length with a polyadenylated tract at the 3' terminus. The structure ...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/s0168-1702(97)00148-2

    authors： Colinet D,Kummert J,Lepoivre P

    更新日期：1998-02-01 00:00:00

  • Development of a mandarin fish Siniperca chuatsi fry cell line suitable for the study of infectious spleen and kidney necrosis virus (ISKNV).

    abstract：:Infectious spleen and kidney necrosis virus (ISKNV) is a typical species of the genus Megalocytivirus in the family Iridoviridae. However, until recently, no suitable piscine cell line is stably susceptible to ISKNV. Here, a continuous cell culture derived from the mandarin fish fry (MFF-1) was developed and has been ...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2008.04.004

    authors： Dong C,Weng S,Shi X,Xu X,Shi N,He J

    更新日期：2008-08-01 00:00:00

  • Coupled mutations in the 5'-untranslated region of the Sabin poliovirus strains during in vivo passages: structural and functional implications.

    abstract：:All entero- and rhinovirus RNAs sequenced thus far possess A and U residues at positions corresponding to nucleotides 480 and 525, respectively, of poliovirus type 1. These two nucleotides have been proposed previously to form a base pair. The single exception to this rule appears to be the Sabin type 1 strain, which ...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(91)90002-d

    authors： Muzychenko AR,Lipskaya GYu,Maslova SV,Svitkin YV,Pilipenko EV,Nottay BK,Kew OM,Agol VI

    更新日期：1991-10-01 00:00:00

  • A sensitive and rapid RNA silencing suppressor activity assay based on alfalfa mosaic virus expression vector.

    abstract：:Plant viruses express RNA silencing suppressor (RSS) proteins to counteract plant defence mechanisms. Here, we describe a method to assess the RSS activity based on an alfalfa mosaic virus (AMV) RNA 3 expression vector and transgenic Nicotiana tabacum plants that express the P1 and P2 subunits of the AMV replicase (P1...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2019.197733

    authors： Martínez-Pérez M,Navarro JA,Pallás V,Sánchez-Navarro JA

    更新日期：2019-10-15 00:00:00

  • Pattern of Epstein-Barr virus (EBV)-specific proteins synthesized during primary lytic infection of mouse lymphocytes by EBV.

    abstract：:Normal mouse lymphocytes were implanted with EBV receptors and exposed to the virus of P3HR-1 strain. 5% of the cells expressed early (EA) and viral capsid (VCA) antigens as assayed by immunofluorescence 24 h after the infection. Only 0.1% of cells expressed nuclear-like antigen (EBNA) 48 h post-infection. When labell...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(84)90016-9

    authors： Sinangil F,Volsky DJ

    更新日期：1984-01-01 00:00:00

  • Differential suppression of host cell protein synthesis and mRNA levels in herpes simplex virus-infected endothelial cells.

    abstract：:Earlier studies from this laboratory have shown that infection of vascular cells with herpes simplex virus 1 or 2 (HSV-1, HSV-2) results in the differential suppression of extracellular matrix proteins including fibronectin (FN), type IV collagen, thrombospondin (TSP) and Factor VIII von Willebrand protein. The presen...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(91)90047-y

    authors： Brinker JM,Ziaie Z,Kefalides NA

    更新日期：1991-05-01 00:00:00

  • Phylogenetic analysis of segment 10 from African horsesickness virus and cognate genes from other orbiviruses.

    abstract：:Utilizing the reverse transcriptase-polymerase chain reaction (RT-PCR) procedure, we have synthesized full-length copies of segment 10 from African horsesickness virus (AHSV) serotypes 1, 4 and 8. The genes were cloned, sequenced and compared with the sequence of the cognate gene from AHSV serotypes 3 and 9. Sequences...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(94)90052-3

    authors： de Sá RO,Zellner M,Grubman MJ

    更新日期：1994-08-01 00:00:00

  • Characterization of the infection of Aedes albopictus cell clones by Sindbis virus.

    abstract：:We have investigated the infection of Aedes albopictus (mosquito) cell clones by Sindbis virus. Variation in the multiplicity of infection (MOI) from ranges of 50-0.00005 pfu/cell was determined to have no effect on the progression of the infection to high acute phase titer, suggesting that intracellular factors alone...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/s0168-1702(97)01461-5

    authors： Karpf AR,Blake JM,Brown DT

    更新日期：1997-07-01 00:00:00

  • Sequence requirements for localization and packaging of Ty3 retroelement RNA.

    abstract：:Retroviruses and retrotransposons package genomic RNA into virus-like particles (VLPs) in a poorly understood process. Expression of the budding yeast retrotransposon Ty3 results in the formation of cytoplasmic Ty3 VLP assembly foci comprised of Ty3 RNA and proteins, and cellular factors associated with RNA processing...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2012.10.008

    authors： Clemens K,Bilanchone V,Beliakova-Bethell N,Larsen LS,Nguyen K,Sandmeyer S

    更新日期：2013-02-01 00:00:00

  • Comparison of the beet necrotic yellow vein virus P75 nucleotide sequences of Belgian type A and type B sources.

    abstract：:RNA-2 P75 open reading frame sequencing was conducted on BNYVV-infected sugar beet plants originating from 10 Belgian sources and was compared with sequences originating from France, Kazakhstan, Japan and China. This allowed the characterization of types A and B in Belgium. Sequence analysis confirmed that type A is i...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2004.07.007

    authors： Meunier A,Schmit JF,Bragard C

    更新日期：2005-03-01 00:00:00

  • Comparing PreXMRV-2 gag sequence diversity in laboratory and wild mice using deep sequencing.

    abstract：:It has recently been reported that the xenotropic murine leukemia virus-related virus (XMRV) derives from a laboratory recombinant. However, sequences with characteristics of the 5' half of XMRV (termed PreXMRV-2) have been identified in several laboratory mouse genomes and cell lines suggesting parts of the XMRV geno...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2012.06.026

    authors： Mayer J,Mazzoni CJ,Greenwood AD

    更新日期：2012-10-01 00:00:00

  • Effect of recombinant hybrid human interferon on replication and morphogenesis of HSV-1 in monkey cells.

    abstract：:Human recombinant alpha interferon, A/D, significantly reduced the replication and cell fusion induced by herpes simplex virus type 1 in monkey cells. Thin-section electron microscopy of interferon-treated monkey cells showed distinct assembly of nucleocapsids within the nucleus. Analysis of virus-specific proteins by...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(89)90051-8

    authors： Chatterjee S,Whitley RJ

    更新日期：1989-01-01 00:00:00

  • Analysis of antibody response to the measles virus using synthetic peptides of the fusion protein. Evidence of non-random pairing of T and B cell epitopes.

    abstract：:The measles virus induces a life-long immune response associated with antibodies specific for the fusion protein. To map the linear immunodominant recognition sites of the fusion (F) protein of the measles virus, we have reacted a complete set of 108 overlapping pentadecapeptides with purified IgG obtained from donor ...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(93)90095-5

    authors： Muller CP,Handtmann D,Brons NH,Weinmann M,Wiesmüller KH,Spahn G,Wiesneth M,Schneider F,Jung G

    更新日期：1993-12-01 00:00:00

  • Viral modulation of stress granules.

    abstract：:Following viral infection, the host responds by mounting a robust anti-viral response with the aim of creating an unfavorable environment for viral replication. As a countermeasure, viruses have elaborated mechanisms to subvert the host response in order to maintain viral protein synthesis and production. In the last ...

    journal_title：Virus research

    pub_type： 杂志文章,评审

    doi：10.1016/j.virusres.2012.06.004

    authors： Valiente-Echeverría F,Melnychuk L,Mouland AJ

    更新日期：2012-11-01 00:00:00

  • Proteins of duck influenza virus responsible for acquisition of pathogenicity in chickens.

    abstract：:Influenza virus rgVac1sub-P0 (H5N1) (rgVac1-P0), in which a pair of dibasic amino acid residues was introduced at the cleavage site of the HA of a reassortant of H5N2 and H7N1 viruses of duck origin, was low pathogenic in chickens. Vac1sub-P3 (H5N1) (Vac1-P3) was selected as a highly pathogenic avian influenza virus b...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2013.02.001

    authors： Yamamoto N,Soda K,Sakoda Y,Okamatsu M,Kida H

    更新日期：2013-05-01 00:00:00

  • A review of studies on animal reservoirs of the SARS coronavirus.

    abstract：:In this review, we summarize the researches on animal reservoirs of the SARS coronavirus (SARS-CoV). Masked palm civets were suspected as the origin of the SARS outbreak in 2003 and was confirmed as the direct origin of SARS cases with mild symptom in 2004. Sequence analysis of the SARS-CoV-like virus in masked palm c...

    journal_title：Virus research

    pub_type： 杂志文章,评审

    doi：10.1016/j.virusres.2007.03.012

    authors： Shi Z,Hu Z

    更新日期：2008-04-01 00:00:00

  • Aberrant expression of liver microRNA in chickens infected with subgroup J avian leukosis virus.

    abstract：:Subgroup J avian leukosis virus (ALV-J) is an oncogenic retrovirus primarily causing myeloid leukosis (ML) in broilers. Although ALV is well under control in a few countries including the USA, poultry industry in many parts of the world continues suffering from serious economic loss due to sporadic or widespread ALV i...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2012.07.003

    authors： Li H,Ji J,Xie Q,Shang H,Zhang H,Xin X,Chen F,Sun B,Xue C,Ma J,Bi Y

    更新日期：2012-10-01 00:00:00

  • A novel, highly divergent ssDNA virus identified in Brazil infecting apple, pear and grapevine.

    abstract：:Fruit trees of temperate and tropical climates are of great economical importance worldwide and several viruses have been reported affecting their productivity and longevity. Fruit trees of different Brazilian regions displaying virus-like symptoms were evaluated for infection by circular DNA viruses. Seventy-four fru...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2015.07.005

    authors： Basso MF,da Silva JC,Fajardo TV,Fontes EP,Zerbini FM

    更新日期：2015-12-02 00:00:00

  • Uptake of porcine parvovirus into host and nonhost cells suggests host specificity is determined by intracellular factors.

    abstract：:The uptake of porcine parvovirus (PPV) into host cells, permissive or nonpermissive for PPV replication, was monitored by autoradiography, immunofluorescent microscopy, and dual parameter flow cytometry. While both permissive and nonpermissive cells selectively took up the light (activated) form of the virus from a mi...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(88)90054-8

    authors： Ridpath JF,Mengeling WL

    更新日期：1988-04-01 00:00:00

  • Fusion of sendai virus with liposome depends on only F protein, but not HN protein.

    abstract：:Sendai virus is able to fuse with liposomes even without virus receptors. To determine the roles of envelope protein, hemagglutinin-neuraminidase (HN) and fusion (F) protein, in Sendai virus-liposome fusion, we treated the virus with proteases and examined its fusion with liposomes and the conditions of HN and F prote...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/s0168-1702(98)00137-3

    authors： Mizuguchi H,Nakanishi T,Kondoh M,Nakagawa T,Nakanishi M,Matsuyama T,Tsutsumi Y,Nakagawa S,Mayumi T

    更新日期：1999-02-01 00:00:00

  • Identification of mixed equine rhinitis B virus infections leading to further insight on the relationship between genotype, serotype and acid stability phenotype.

    abstract：:Equine rhinitis B virus (ERBV) is the single species in the genus Erbovirus, family Picornaviridae. Equine rhinitis B viruses exist in three serotypes and are associated with respiratory disease in horses. Members of the species vary in stability at acid pH. To date there has been discordance in genotype, serotype and...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2010.12.007

    authors： Horsington JJ,Gilkerson JR,Hartley CA

    更新日期：2011-02-01 00:00:00

  • The novel hemagglutinin-esterase genes of human torovirus and Breda virus.

    abstract：:Human torovirus (HTV) and Breda virus (BRV), members of the genus torovirus in the family Coronaviridae, are established infectious agents of humans and cattle, respectively. The hemagglutinin-esterase (HE) gene of Breda virus serotype 2 (BRV-2) has been identified and the nucleotide sequence for BRV serotype 1 (BRV-1...

    journal_title：Virus research

    pub_type： 杂志文章,收录出版

    doi：10.1016/s0168-1702(99)00088-x

    authors： Duckmanton L,Tellier R,Richardson C,Petric M

    更新日期：1999-11-01 00:00:00

  • Molecular characterization of begomoviruses and DNA satellites associated with okra leaf curl disease in Cameroon.

    abstract：:Okra leaf curl disease (OLCD) is the most important viral disease of okra in West Africa. In this study, a complex of begomoviruses and associated DNA satellites were identified in symptomatic okra plants from southwestern Cameroon. Sequence analyses showed that two of the plants (Lik1 and Njo5) were infected with a b...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2013.03.010

    authors： Leke WN,Sattar MN,Ngane EB,Ngeve JM,Kvarnheden A,Brown JK

    更新日期：2013-06-01 00:00:00