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A sensitive and rapid RNA silencing suppressor activity assay based on alfalfa mosaic virus expression vector.

Abstract：

:Plant viruses express RNA silencing suppressor (RSS) proteins to counteract plant defence mechanisms. Here, we describe a method to assess the RSS activity based on an alfalfa mosaic virus (AMV) RNA 3 expression vector and transgenic Nicotiana tabacum plants that express the P1 and P2 subunits of the AMV replicase (P12 plants). Inoculation of P12 plants with different AMV RNA 3 constructs expressing different HC-Pro mutants that differ in their RSS capabilities, revealed a perfect correlation between necrotic lesions on inoculated leaves and RSS activity. Protoplast analysis showed that the RSS activity correlated with the accumulation of the AMV RNAs. A direct comparison between three RSS activity assays and the AMV-P12 system revealed that the coat protein of carnation mottle virus displays RSS activity with the four assays and reduced the accumulation of the siRNAs.

journal_name

Virus Res

journal_title

Virus research

authors

Martínez-Pérez M,Navarro JA,Pallás V,Sánchez-Navarro JA

doi

10.1016/j.virusres.2019.197733

subject

Has Abstract

pub_date

2019-10-15 00:00:00

pages

197733

eissn

0168-1702

issn

1872-7492

pii

S0168-1702(19)30399-5

journal_volume

272

pub_type

杂志文章

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