A rapid method for the analysis of influenza virus genes: application to the reassortment of equine influenza virus genes.

Abstract:

:We describe a rapid method for genetic characterisation of influenza virus genes using reverse transcription and amplification by polymerase chain reaction (RT/PCR) of all virus segments simultaneously (multiplex RT/PCR) using primers based on the conserved terminal sequences. The product has been shown to be suitable for determination of partial nucleotide sequences which can be used to search nucleotide sequence databases and rapidly map the genetic origin of each segment. We illustrate the use of the method by analysing genetic reassortment in H7N7 equine influenza viruses.

journal_name

Virus Res

journal_title

Virus research

authors

Adeyefa CA,Quayle K,McCauley JW

doi

10.1016/0168-1702(94)90087-6

subject

Has Abstract

pub_date

1994-06-01 00:00:00

pages

391-9

issue

3

eissn

0168-1702

issn

1872-7492

pii

0168-1702(94)90087-6

journal_volume

32

pub_type

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