Abstract:
:We describe a rapid method for genetic characterisation of influenza virus genes using reverse transcription and amplification by polymerase chain reaction (RT/PCR) of all virus segments simultaneously (multiplex RT/PCR) using primers based on the conserved terminal sequences. The product has been shown to be suitable for determination of partial nucleotide sequences which can be used to search nucleotide sequence databases and rapidly map the genetic origin of each segment. We illustrate the use of the method by analysing genetic reassortment in H7N7 equine influenza viruses.
journal_name
Virus Resjournal_title
Virus researchauthors
Adeyefa CA,Quayle K,McCauley JWdoi
10.1016/0168-1702(94)90087-6subject
Has Abstractpub_date
1994-06-01 00:00:00pages
391-9issue
3eissn
0168-1702issn
1872-7492pii
0168-1702(94)90087-6journal_volume
32pub_type
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