Abstract:
:SARS-CoV 3a protein was a unique protein of SARS coronavirus (SARS-CoV), which was identified in SARS-CoV infected cells and SARS patients' specimen. Recent studies revealed that 3a could interact specifically with many SARS-CoV structural proteins, such as M, E and S protein. Expressed 3a protein was reported to localize to Golgi complex in SARS-CoV infected cells. In this study, it was shown that 3a protein was mainly located in Golgi apparatus with different tags at N- or C-terminus. The localization pattern was similar in different transfected cells. With the assay of truncated 3a protein, it was shown that 3a might contain three transmembrane regions, and the second or third region was properly responsible for Golgi localization. By ultra-centrifugation experiment with different extraction buffers, it was confirmed that 3a was an integral membrane protein and embedded in the phospholipid bilayer. Immunofluorescence assay indicated that 3a was co-localized with M protein in Golgi complex in co-transfected cells. These results provide a new insight for further study of the 3a protein on the pathogenesis of SARS-CoV.
journal_name
Virus Resjournal_title
Virus researchauthors
Yuan X,Li J,Shan Y,Yang Z,Zhao Z,Chen B,Yao Z,Dong B,Wang S,Chen J,Cong Ydoi
10.1016/j.virusres.2005.01.001subject
Has Abstractpub_date
2005-05-01 00:00:00pages
191-202issue
2eissn
0168-1702issn
1872-7492pii
S0168-1702(05)00002-Xjournal_volume
109pub_type
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