The human papillomavirus18 E7 protein inhibits CENP-C binding to α-satellite DNA.

Abstract:

:Human papillomavirus (HPV) infection leads to aneuploidy, a numerical chromosomal aberration that is caused by dysregulation of chromosomal segregation. We previously found that the E7 proteins of high-risk HPVs, but not of low-risk HPVs, could bind to centromere protein-C (CENP-C). In this study, we first found that CENP-C could bind centromere α-satellite DNAs using ChIP analysis and HA-tagged CENP-C/nuc transfected 293T cells. We then investigated if HA-CENP-C/nuc binding to α-satellite DNAs was affected by the E7 proteins of high- or low-risk HPVs. We found that transfection of the FLAG tagged HPV18 E7 inhibited the binding of HA-CENP-C/nuc to α-satellite DNAs. This finding was confirmed in HeLa S3 cells transfected with siRNA targeted to HPV18 E7 expression. We therefore speculate that altered function of kinetochores as a result of inhibition of CENP-C and α-satellite DNAs binding may be associated with the chromosomal abnormalities observed in HPV18-positive cancers.

journal_name

Virus Res

journal_title

Virus research

authors

Yaginuma Y,Yoshimoto M,Eguchi A,Tokuda A,Takahashi S

doi

10.1016/j.virusres.2015.04.019

subject

Has Abstract

pub_date

2015-07-02 00:00:00

pages

27-32

eissn

0168-1702

issn

1872-7492

pii

S0168-1702(15)00171-9

journal_volume

205

pub_type

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