Abstract：
:Several cell surface molecules have been implicated in rotavirus cell entry, however, their individual relevance during this process is unknown. In this work, the expression of integrins alpha2, beta2, and alpha v beta 3, the heat shock cognate protein 70, and of ganglioside GM1 in different cell lines of human and simian origin was correlated with the infectivity of four rotavirus strains. We observed that different combinations of receptor expression correlated with the infectivity of rotavirus strains, suggesting that the participation of several receptors is important for rotavirus infection. To characterize the relevance of integrins alpha2 and alpha v beta 3 in more detail, their expression was silenced using RNA interference. About 80% decrease in the cell content of integrins resulted in 15-30% decrease of infectivity of strains RRV and Wa when measured by a focus-forming assay, while there was no decrease of infectivity when measured by flow cytometry in integrin-deficient cells. Altogether these data suggest that integrins alpha2 and alpha v beta 3 do not play a major role in the rotavirus entry process.
journal_name
Virus Resjournal_title
Virus researchauthors
Isa P,Sánchez-Alemán MA,López S,Arias CFdoi
10.1016/j.virusres.2009.07.013subject
Has Abstractpub_date
2009-11-01 00:00:00pages
251-9issue
2eissn
0168-1702issn
1872-7492pii
S0168-1702(09)00248-2journal_volume
145pub_type
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