Abstract:
:The fidelity of poliovirus RNA-dependent RNA polymerase (3D(pol)) was determined using a system based on the fidelity of synthesis of the alpha-lac gene which codes for a subunit of beta-galactosidase. Synthesis products are screened for mutations by an alpha-complementation assay, in which the protein product from alpha-lac is used in trans to complement beta-galactosidase activity in bacteria that do not express alpha-Lac. Several polymerases have been analyzed by this approach allowing comparisons to be drawn. The assay included RNA synthesis by 3D(pol) on an RNA template that coded for the N-terminal region of alpha-Lac. The product of this reaction was used as a template for a second round of 3D(pol) synthesis and the resulting RNA was reverse transcribed to DNA by MMLV-RT. The DNA was amplified by PCR and inserted into a vector used to transform Escherichia coli. The bacteria were screened for beta-galactosidase activity by blue-white phenotype analysis with white or faint blue colonies scored as errors made during synthesis on alpha-lac. Results showed a mutation rate for 3D(pol) corresponding to approximately 4.5x10(-4) errors per base (one error in approximately 2200 bases). Analysis of mutations showed that base substitutions occurred with greater frequency than deletions and insertions.
journal_name
Virus Resjournal_title
Virus researchauthors
Wells VR,Plotch SJ,DeStefano JJdoi
10.1016/s0168-1702(00)00256-2subject
Has Abstractpub_date
2001-04-01 00:00:00pages
119-32issue
1-2eissn
0168-1702issn
1872-7492pii
S0168170200002562journal_volume
74pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract::Influenza A viruses are characterized by a unique genome structure, causing genetic instability, especially to the genes of haemagglutinin and neuraminidase. The objectives of this research was molecular and phylogenetic analysis of influenza A(H3N2) strains that circulated in Northern Greece since 2004, particularly ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2009.07.006
更新日期:2009-11-01 00:00:00
abstract::A cloned cDNA (1.65 kb) containing the complete glycoprotein gene of the Josiah strain of Lassa virus was inserted into the thymidine kinase (TK) gene of the New York Board of Health (WYETH) strain of vaccinia virus. The Lassa virus glycoprotein precursor, GPC, and the posttranslational cleavage products, G1 and G2, w...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(88)90033-0
更新日期:1988-02-01 00:00:00
abstract::Cold-passaged (CP) mutants derived from the JS strain of wild type wt parainfluenza type 3 virus (PIV3) are being evaluated as candidate live virus vaccines. The wt virus was serially passaged 45 times at low temperature and mutant clones with the cold-adapted (CA), temperature-sensitive (ts), and attenuation (ATT) ph...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(92)90049-f
更新日期:1992-03-01 00:00:00
abstract::The knowledge of parainfluenza type 5 (PIV-5) virion morphology is essentially based on the observation of negatively stained preparations in conventional transmission electron microscopy (CTEM). In this study, the ultrastructure of frozen-hydrated intact PIV-5 was examined by cryo-electron microscopy (cryo-EM). Cryo-...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2008.12.017
更新日期:2009-06-01 00:00:00
abstract::A double-stranded (ds) RNA, approximately 9.5kb in size; was identified in the MVC86 isolate of Valsa ceratosperma. Complete sequence of the dsRNA revealed a 9543-bp segment (excluding the 3' poly-A tail) that is predicted to encode a single large protein (P330). P330 has 63%, 49%, and 55% amino acid sequence identiti...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.02.008
更新日期:2012-05-01 00:00:00
abstract::Positive-strand RNA viruses exist as a quasi-species due to the incorporation of mutations into the viral genome during replication by the virus-encoded RNA-dependent RNA polymerase (RdRP). Therefore, the RdRP is often described as a low-fidelity enzyme. However, until recently, a complete description of the kinetic, ...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2004.11.004
更新日期:2005-02-01 00:00:00
abstract::Poliovirus (PV) infects susceptible cells through poliovirus receptor (PVR), which functions to bind virus and to convert its conformation. To study early infection process of PV, infection systems were employed using in vitro cultured cells and in vivo neural pathway of PVR transgenic (Tg) mice. For in vitro study, m...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(99)00040-4
更新日期:1999-08-01 00:00:00
abstract::Utilizing the reverse transcriptase-polymerase chain reaction (RT-PCR) procedure, we have synthesized full-length copies of segment 10 from African horsesickness virus (AHSV) serotypes 1, 4 and 8. The genes were cloned, sequenced and compared with the sequence of the cognate gene from AHSV serotypes 3 and 9. Sequences...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(94)90052-3
更新日期:1994-08-01 00:00:00
abstract::Human respiratory syncytial virus (HRSV) P protein, 241 amino acid long, is a structural homotetrameric phosphoprotein. Viral transcription and replication processes are dependent on functional P protein interactions inside viral ribonucleoprotein complexes (RNPs). Binding capacity to RNPs proteins and transcription a...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2007.11.013
更新日期:2008-03-01 00:00:00
abstract::The role of the C2 protein in the pathogenicity of tomato yellow leaf curl Sardinia virus (TYLCSV) was investigated. Here we report that Agrobacterium-mediated transient expression of TYLCSV C2 resulted in a strong hypersensitive response (HR) in Nicotiana benthamiana, N. tabacum, and Arabidopsis thaliana, with induct...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2016.01.014
更新日期:2016-04-02 00:00:00
abstract::Previous investigations suggest that the divergence of the group I alphabaculoviruses was later than that of the group II alphabaculoviruses, however, there is no quantitative data to support this hypothesis. To examine this theory, the evolutionary rates of the 30 core genes that are conserved among all baculoviruses...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2009.01.015
更新日期:2009-06-01 00:00:00
abstract::Several cell surface molecules have been implicated in rotavirus cell entry, however, their individual relevance during this process is unknown. In this work, the expression of integrins alpha2, beta2, and alpha v beta 3, the heat shock cognate protein 70, and of ganglioside GM1 in different cell lines of human and si...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2009.07.013
更新日期:2009-11-01 00:00:00
abstract::Cricket paralysis virus (Dicistroviridae: Cripavirus) (CrPV) naturally has a wide range of insect hosts which is reflected in its ability to infect several cultured insect cell lines. The expression of viral gene products is controlled by two kinds of internal ribosome entry site (IRES) elements, 5' and intergenic (IG...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(03)00139-4
更新日期:2003-08-01 00:00:00
abstract::RNA species of plant tripartite RNA viruses show distinct translational activities in vitro when the viral RNA concentration is high. However, it is not known what causes the differential translation of virion RNAs. Using an in vitro wheat germ translation system, we investigated the translation efficiencies and compe...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(00)00120-9
更新日期:2000-02-01 00:00:00
abstract::Transplacental viral infections are dependent upon complex interactions between feto-placental and maternal immune responses and the stage of fetal development at which the infection occurs. Bovine viral diarrhea virus (BVDV) has the ability to cross the placenta and infect the fetus. Infection early in gestation with...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.05.018
更新日期:2012-08-01 00:00:00
abstract::Infectious spleen and kidney necrosis virus (ISKNV) is the etiological agent that causes a pandemic and severe disease in fish characteristic of enlarged and damaged spleen and kidney. To identify viral proteins involved in infection and pathogenesis, we characterized five open reading frames (ORFs) of the ISKNV genom...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2006.09.003
更新日期:2007-02-01 00:00:00
abstract::Tick-borne encephalitis (TBE) is a serious human neurological disease caused by TBE virus (TBEV). However, the mechanisms of TBEV-caused pathogenesis remain unclear. The endoplasmic reticulum (ER) stress response, also defined as the unfolded protein response (UPR), is an important conserved molecular signaling pathwa...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2013.10.012
更新日期:2013-12-26 00:00:00
abstract::RNA-2 P75 open reading frame sequencing was conducted on BNYVV-infected sugar beet plants originating from 10 Belgian sources and was compared with sequences originating from France, Kazakhstan, Japan and China. This allowed the characterization of types A and B in Belgium. Sequence analysis confirmed that type A is i...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2004.07.007
更新日期:2005-03-01 00:00:00
abstract::Experimental infection studies with bluetongue virus (BTV) in the mammalian host have a history that stretches back to the late 18th century. Studies in a wide range of ruminant and camelid species as well as mice have been instrumental in understanding BTV transmission, bluetongue (BT) pathogenicity/pathogenesis, vir...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2013.12.044
更新日期:2014-03-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) is characteristic of genetically extensive variation. In this study, five SPF pigs were co-infected with two strains of PRRSV (JXwn06-81c and HB-1/3.9c), and 352 viruses were cloned by plaque assay from the sera of the infected pigs on days 3, 5, 7, 10, 14, 2...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2010.12.003
更新日期:2011-02-01 00:00:00
abstract::The porcine serum inhibitor alpha 2-macroglobulin prevents influenza virus from entering host cells by competing for the SA alpha 2, 6Gal-binding site of the hemagglutinin (HA). We studied a series of inhibitor-sensitive and inhibitor-resistant human and porcine influenza virus isolates of the H3N2 subtype, all of whi...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(96)01304-4
更新日期:1996-06-01 00:00:00
abstract::Variants assigned to GI-23 lineage of infectious bronchitis virus (IBV), formerly called Var2, have circulated for nearly 20 years only in countries of the Middle East. Strains of this lineage were first identified in Israel in 1998. More severe form of the virus appeared in 2006, when the second wave of Var2 epidemic...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2017.09.010
更新日期:2017-10-15 00:00:00
abstract::We previously established inducible-hepatitis C virus (HCV) transgenic mice, which expressed the HCV gene (nucleotides 294-3435) encoding the core, E1, E2, and NS2 proteins. The expression of these proteins is regulated by the Cre/loxP system and an adenovirus vector (AdV) that expresses Cre DNA recombinase (Cre) cont...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2011.05.019
更新日期:2011-09-01 00:00:00
abstract::Aphid species were counted on citrus trees in orchards in Valencia, Spain, in the spring and autumn of 1997, 1998 and 1999. Moericke yellow water traps, the 'sticky shoot' method and counts of established colonies were used in extensive surveys in which 29,502 aphids were recorded and identified. Aphis spiraecola and ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2003.12.018
更新日期:2004-03-01 00:00:00
abstract::Hepatitis C virus (HCV) is a modern-day pandemic; 2-3% of the world's population are thought to be infected with the virus and are subsequently at risk of developing end-stage liver diseases. The traditional standard of care (SOC) for HCV-infected patients has been limited to a regimen of pegylated-interferon alpha (p...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2012.09.007
更新日期:2012-12-01 00:00:00
abstract::The aim of the present study was to analyze the influence of virus origin, mammalian or mosquito cell-derived, on antiviral susceptibility of DENV-2 to entry inhibitors and the association of this effect with any alteration in the mode of entry into the cell. To this end, ten serial passages of DENV-2 were performed i...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2014.02.011
更新日期:2014-05-12 00:00:00
abstract::To investigate the genetic variability and evolution of rabbit hemorrhagic disease virus (RHDV) strains in China, VP60 gene sequences of eight new isolates collected from farms with RHD occurrences in China between 2009 and 2014 were analyzed, and compared with the reference sequence of the vaccine strain WF/China/200...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2016.01.018
更新日期:2016-04-02 00:00:00
abstract::African horse sickness (AHS) is a devastating viral disease affecting equines and has resulted in many disastrous epizootics. To date, no successful therapeutic treatment exists for AHS, and commercially used live-attenuated vaccines have various undesirable side effects. Previous studies have shown that mice inoculat...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2020.198284
更新日期:2021-01-06 00:00:00
abstract::Genome organization and gene expression of Borna disease virus (BDV) are remarkable for the overlap of open reading frames, transcription units and transcription signals, readthrough of transcription termination signals, differential use of translation initiation codons, and exploitation of the cellular splicing machi...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2009.03.016
更新日期:2009-09-01 00:00:00
abstract::Dengue (Den) viruses cause apoptosis in mammalian cells, but usually result in high progeny yields without evident damage in mosquito cells. By using subtractive hybridization, 13 potentially virus-induced genes were selected in Den-2 virus-infected Aedes albopictus C6/36 cells. Based on semi-quantitative and real-tim...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2006.11.002
更新日期:2007-03-01 00:00:00