Abstract：
:We have found that an HIV-1 accessory gene product Vpr enhanced HIV-1 reproduction in U1 cells chiefly by the induction of TNF, a proinflammatory cytokine, which was also known to be an activator of HIV-1 reproduction. We have generated the functional HIV-1 accessory gene product Vpr in bacterial cells. Vpr was generated in an Escherichia coli system (rVpr), purified with antibodies (Ab) to the 16 C-terminal amino acids of Vpr. The purified rVpr of 15 kDa was examined for its ability to upregulate HIV-1 reproduction in U1 cells, which is a reported function of the authentic Vpr. rVpr upregulated HIV-1 reproduction in U1 cells in a dose-dependent manner and induced the secretion of TNF. The upregulation of HIV-1 by rVpr was completely inhibited not only by anti-Vpr antibodies but also by anti-TNF antibody. These findings suggested that Vpr caused an HIV-1 reproduction in U1 cells through the induction of TNF.
journal_name
Virus Resjournal_title
Virus researchauthors
Nakamura T,Suzuki H,Okamoto T,Kotani S,Atsuji Y,Tanaka T,Ito Ydoi
10.1016/s0168-1702(02)00230-7subject
Has Abstractpub_date
2002-12-01 00:00:00pages
263-8issue
1-2eissn
0168-1702issn
1872-7492pii
S0168170202002307journal_volume
90pub_type
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