Abstract:
:The phylogenetic diversity of classical swine fever virus (CSFV) in China has been extensively studied previously, with the report of the classification of Chinese CSFVs into four subgroups within two of the established genotypes, but the antigenic differences amongst Chinese CSF viruses still remain unknown. To address this issue, 21 CSFV field strains isolated in China between 1996 and 2006 were grown in cell culture and characterized in comparison with two Chinese reference strains: a virulent strain Shimen and a vaccine strain CSF lapinized virus (hog cholera lapinized virus in China, HCLV), by indirect immunofluorescence assay (IFA) with a panel of 28 monoclonal antibodies (mAbs) against four pestiviruses, CSFV, bovine viral diarrhoea virus-1 (BVDV-1), bovine viral diarrhoea virus-2 (BVDV-2) and border disease virus (BDV). All 23 CSFV strains reacted only with CSFV-specific mAbs, not with those raised against BVDV-1, BVDV-2 and BDV. Of the former mAbs, those directed against CSFV E2 protein recognized more isolates than those directed against E(rns) and NS2/3. Of nine CSFV E2-specific mAbs used, WH303 and WH302 reacted with all 23 strains, confirming their value in differentiating CSFV from other pestiviruses. Furthermore, different strains had different patterns of reactivity with CSFV-specific mAbs, and mAbs other than WH303 and WH302 did not recognize all strains. This study provides the first evidence for the existence of antigenic differences among Chinese CSFVs.
journal_name
Virus Resjournal_title
Virus researchauthors
Zhu Y,Shi Z,Drew TW,Wang Q,Qiu H,Guo H,Tu Cdoi
10.1016/j.virusres.2009.02.011subject
Has Abstractpub_date
2009-06-01 00:00:00pages
169-74issue
1-2eissn
0168-1702issn
1872-7492pii
S0168-1702(09)00069-0journal_volume
142pub_type
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