Abstract：
:PCR amplification of proviral DNA extracted from peripheral blood lymphocytes of three Chinese pigs (Banna minipig inbreed (BMI), Wu-Zhi-Shan pig (WZSP) and Neijiang pig (NJP)), using primers corresponding to highly conserved regions of reverse transcriptase (RT) of pol gene and nucleocapsid sequence of gag gene. PCR products were then extracted and cloned into pGEM-T vector. Phylogenetic analysis of the nucleotide sequences of PERV-BMI, PERV-WZSP and PERV-WZSP revealed that they were of retroviral origin. Phylogenetic trees were constructed from the translated amino acids of PERVs and other type C retrovirus, as well as lentivirus of GenBank. The research demonstrated that PERVs of Chinese pigs and other PERVs were closely related to other pathogenic type C retroviruses. From the gag analysis, a novel subgroup of PERV was identified and this novel sequence described in this report would allow such investigation to be actively pursued.
journal_name
Virus Resjournal_title
Virus researchauthors
Li Z,Ping Y,Shengfu L,Hong B,Youping L,Yangzhi Z,Jingqiu Cdoi
10.1016/j.virusres.2004.05.007subject
Has Abstractpub_date
2004-10-01 00:00:00pages
167-73issue
2eissn
0168-1702issn
1872-7492pii
S0168170204002461journal_volume
105pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract：:One hundred and twenty-four rabies viruses (RABV) were isolated from humans and eight species of mammals in Colombia during 1994-2005. To determine the genetic and reservoir-associated diversity cDNA fragments encoding 88 amino acids at the carboxyl terminus of the nucleoprotein were sequenced and used in phylogenetic...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2007.06.008
更新日期：2007-12-01 00:00:00
abstract：:Post-translational modification of target proteins by the Small Ubiquitin-like Modifier (SUMO) plays a critical role in regulation of many cellular processes including transcription, RNA processing, protein trafficking, DNA repair, and chromosome segregation, and is also often hijacked by viral infections. Epstein-Bar...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2020.197962
更新日期：2020-07-02 00:00:00
abstract：:Prion diseases or Transmissible Spongiform Encephalopathies (TSEs) are a group of fatal neurodegenerative disorders affecting several mammalian species being Creutzfeldt-Jacob Disease (CJD) the most representative in human beings, scrapie in ovine, Bovine Spongiform Encephalopathy (BSE) in bovine and Chronic Wasting D...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2015.04.014
更新日期：2015-09-02 00:00:00
abstract：:In this review, we discuss possible models of bacteriophage-virus interactions. The first is based on the mechanism by which phages may interact indirectly with viruses. Its essence is that bacteriophage-derived nucleic acid may inhibit pathogenic virus infection. It seems that this phenomenon can be partly explained ...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2005.01.009
更新日期：2005-06-01 00:00:00
abstract：:The immune response to a naked plasmid DNA encoding the nucleoprotein (N protein) of porcine transmissible gastroenteritis virus (TGEV) was investigated in this study. A complementary DNA of the entire N gene was amplified by RT-PCR, and inserted into a mammalian expression vector (pcDNA3.1) to construct a recombinant...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(01)00333-1
更新日期：2001-11-28 00:00:00
abstract：:Human metapneumovirus (hMPV) is a major cause of respiratory tract infections in children, elderly and immunocompromised hosts, for which no vaccine or treatment are currently available. Oxidative stress and inflammatory responses represent important pathogenic mechanism(s) of hMPV infection. Here, we explored the pot...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2015.01.018
更新日期：2015-03-16 00:00:00
abstract：:Serological parameters intend to describe antibody response to influenza vaccine in a population. However, there is uncertainty about the mathematical appropriateness and the biological or clinical meaning of conventionally used parameters. Theoretical considerations and exploration of a data-set of 16 studies with an...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.02.024
更新日期：2004-07-01 00:00:00
abstract：:Caprine herpesvirus 1 (CpHV-1) is responsible of systemic infection in neonatal kids as well as abortion and fertility disorders in adult goats. This virus is closely related to bovine herpesvirus 1 (BoHV-1) which causes infectious bovine rhinotracheitis. Glycoprotein D (gD) mediates important functions in alphaherpes...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2005.07.009
更新日期：2006-02-01 00:00:00
abstract：:Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis, a malignant B cell lymphoma. BLV has spread worldwide and causes serious problems. After infection, the BLV genome is integrated into the host DNA and can be amplified during periods of latency. We previously designed degenerate primers us...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2015.08.020
更新日期：2015-12-02 00:00:00
abstract：:We have developed a system for the preparation of La Crosse virus (LAC) and Hantaan virus (HTN) pseudotypes using a murine leukemia virus vector. After concentration, the pseudotypes were present in quantities sufficient to analyze cell tropism and neutralization. Cells resistant to LAC could not be infected with the ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(99)00070-2
更新日期：1999-10-01 00:00:00
abstract：:Hepatitis E is acute hepatitis caused by infection of hepatitis E virus (HEV) via a fecal-to-oral or zoonotic route. HEV is a small, non-enveloped virus containing positive strand RNA as a genome. Recently, the three-dimensional structures of the HEV-like particles and spike domain protruded from the surface of the pa...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2011.03.015
更新日期：2011-10-01 00:00:00
abstract：:Canine distemper virus (CDV) is a major pathogen of dogs and represents a serious threat to both unvaccinated and vaccinated animals. This study surveyed dogs with or without clinical signs related to canine distemper from different regions of Brazil from 2008 to 2012. A total of 155 out of 386 animals were found to b...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.12.024
更新日期：2014-02-13 00:00:00
abstract：:The resurgence of Chikungunya virus (CHIKV) in the form of unprecedented explosive epidemic after a gap of 32 years in India is a point of major public health concern. In 2007 again there was outbreak in Kerala, India, affecting more than 25,000 cases with many reported mortalities. To understand the molecular basis o...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.02.004
更新日期：2008-07-01 00:00:00
abstract：:Tomato spotted wilt virus (TSWV) is a plant-infecting member of the family Bunyaviridae. TSWV encodes two envelope glycoproteins, G(N) and G(C), which are required for virus infection of the arthropod vector. Other members of the Bunyaviridae enter host cells by pH-dependent endocytosis. During this process, the glyco...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2005.01.007
更新日期：2005-06-01 00:00:00
abstract：:With the aim to detect what kind of cells, in addition to erythroid progenitors, could be involved in the pathogenesis of B19 infection in some connective tissue diseases, primary cultures of human fibroblasts (HF) and endothelial cells (HUVEC) were exposed to a B19 positive serum (350 genome copies/cell). The presenc...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2005.05.003
更新日期：2005-12-01 00:00:00
abstract：:Porcine reproductive and respiratory syndrome (PRRS) is a major disease affecting pigs worldwide and resulting in considerable economic losses. While PRRS is a global phenomenon, the causative viruses PRRSV-1 (first detected in Europe) and PRRSV-2 (isolated in North America) are genetically and biologically distinct. ...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2014.12.026
更新日期：2015-04-16 00:00:00
abstract：:Porcine reproductive and respiratory syndrome virus (PRRSV) induces apoptosis in cultured cells and in infected tissues of pig, and the GP5 protein was reported to be responsible. To study the role of apoptosis during PRRSV infection, we established a subline of HeLa cells stably expressing the PRRSV GP5 protein under...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.03.001
更新日期：2004-08-01 00:00:00
abstract：:A sero-epidemiologic survey of 74 IVDUs and 86 age-matched controls was performed to investigate the prevalence, clinical significance, and genetic distribution of GB virus C (GBV-C)/hepatitis G virus (HGV) infection among intravenous drug users (IVDUs) in Japan. GBV-C/HGV RNA was detected by reverse transcriptase-hem...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(97)01470-6
更新日期：1997-06-01 00:00:00
abstract：:A 50-kDa protein that binds to viral particles in solid-phase assays and that is recognized by anti-idiotypic antibodies made against anti-viral glycoproteins G1/G2 (anti-Ids) has been proposed as a receptor candidate for tomato spotted wilt tospovirus (TSWV) in its main thrips vector, Frankliniella occidentalis Perga...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(00)00123-4
更新日期：2000-04-01 00:00:00
abstract：:Enterovirus 71 (EV71) is a single-stranded RNA virus that belongs to Picornaviridae family. It causes the hand-foot-and-mouth disease and fatal neurological diseases in young children and infants. The mechanism of EV71 pathogenesis remains obscure. The intestinal tract is the initial site of EV71 replication, but no o...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.05.002
更新日期：2013-09-01 00:00:00
abstract：:A putative nucleoside triphosphate phosphohydrolase (NTPase) gene of African swine fever virus was identified by using a degenerate oligonucleotide probe derived from the nucleoside triphosphate binding motif, which is highly conserved among viral and cellular NTPases. The probe hybridized with fragments SalI E and Ec...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(93)90016-g
更新日期：1993-10-01 00:00:00
abstract：:The recent discovery of a wide range of henipavirus-like viruses circulating in Megabats in Africa raises the question as to the zoonotic potential of these pathogens given the high human mortality rates seen with their pathogenic relatives Nipah virus and Hendra virus. In the absence of cultured infectious African He...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.01.003
更新日期：2014-03-06 00:00:00
abstract：:The broad bean strain of Tobacco mosaic virus (TMV-B) infects Nicotiana tabacum White Burley systemically whereas the tomato strain of T. mosaic virus (ToMV-S1) induces necrotic local lesions and is restricted to inoculated leaves. To examine the possible role of the viral movement protein (MP) in these symptom differ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(02)00083-7
更新日期：2002-07-01 00:00:00
abstract：:Ranaviruses (family Iridoviridae, genus Ranavirus) have been recognized as emerging infectious pathogens and caused a great loss to the global biodiversity. Thymidine kinase (TK) and deoxyuridine triphosphatase (dUTPase, DUT, encoded by ORF 67R) are ubiquitous, existing in iridoviruses and other organisms. Previous st...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.01.008
更新日期：2016-03-02 00:00:00
abstract：:Fourteen isolates of bovine herpesvirus 1 (BHV-1) found representative of more than 100 isolates studied, were compared by restriction fragment pattern analyses and molecularly characterized. A number of evolutionary links between the variants originally associated with infectious bovine rhinotracheitis and the varian...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(96)01383-4
更新日期：1996-12-01 00:00:00
abstract：:The knowledge of parainfluenza type 5 (PIV-5) virion morphology is essentially based on the observation of negatively stained preparations in conventional transmission electron microscopy (CTEM). In this study, the ultrastructure of frozen-hydrated intact PIV-5 was examined by cryo-electron microscopy (cryo-EM). Cryo-...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.12.017
更新日期：2009-06-01 00:00:00
abstract：:The paramyxo- and pneumoviruses are members of the order Mononegavirales, a group of viruses with non-segmented, negative strand RNA genomes. The polymerases of these viruses are multi-functional complexes, capable of transcribing subgenomic capped and polyadenylated mRNAs and replicating the genome. Although there is...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2017.01.008
更新日期：2017-04-15 00:00:00
abstract：:Forty Chinese PCV2 strains collected between 2004 and 2008 were sequenced and their genetic variations were analyzed together with nine previous PCV2 isolates. Phylogenetic analysis indicated that these Chinese PCV2 strains could be divided into four genotypes (PCV-2a, PCV-2b, PCV-2d and PCV-2e), and the genotype PCV-...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.05.015
更新日期：2009-10-01 00:00:00
abstract：:Several cell surface molecules have been implicated in rotavirus cell entry, however, their individual relevance during this process is unknown. In this work, the expression of integrins alpha2, beta2, and alpha v beta 3, the heat shock cognate protein 70, and of ganglioside GM1 in different cell lines of human and si...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.07.013
更新日期：2009-11-01 00:00:00
abstract：:Infectious spleen and kidney necrosis virus (ISKNV) is the etiological agent that causes a pandemic and severe disease in fish characteristic of enlarged and damaged spleen and kidney. To identify viral proteins involved in infection and pathogenesis, we characterized five open reading frames (ORFs) of the ISKNV genom...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2006.09.003
更新日期：2007-02-01 00:00:00
