Abstract：
:Human metapneumovirus (hMPV) is a major cause of respiratory tract infections in children, elderly and immunocompromised hosts, for which no vaccine or treatment are currently available. Oxidative stress and inflammatory responses represent important pathogenic mechanism(s) of hMPV infection. Here, we explored the potential protective role of dietary antioxidants in hMPV infection. Treatment of airway epithelial cells with resveratrol and quercetin during hMPV infection significantly reduced cellular oxidative damage, inflammatory mediator secretion and viral replication, without affecting viral gene transcription and protein synthesis, indicating that inhibition of viral replication occurred at the level of viral assembly and/or release. Modulation of proinflammatory mediator expression occurred through the inhibition of transcription factor nuclear factor (NF)-κB and interferon regulatory factor (IRF)-3 binding to their cognate site of endogenous gene promoters. Our results indicate the use of dietary antioxidants as an effective treatment approach for modulating hMPV induced lung oxidative damage and inflammation.
journal_name
Virus Resjournal_title
Virus researchauthors
Komaravelli N,Kelley JP,Garofalo MP,Wu H,Casola A,Kolli Ddoi
10.1016/j.virusres.2015.01.018subject
Has Abstractpub_date
2015-03-16 00:00:00pages
19-23eissn
0168-1702issn
1872-7492pii
S0168-1702(15)00028-3journal_volume
200pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract：:Northern blot analysis revealed the presence of varicella-zoster virus (VZV) gene 27 transcripts in infected cells. The Sal I-G DNA fragment, located in the unique long segment of the VZV genome and containing overlapping genes 26 and 27, was analyzed in an in vitro transcription-translation system. Translation of RNA...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(88)90015-9
更新日期：1988-05-01 00:00:00
abstract：:Reports of human infections with highly pathogenic H5N1 avian influenza viruses in many countries in Asia and Africa with varying case fatality rates highlight the pandemic potential of these viruses. In order to contain a rapidly spreading influenza virus in a pandemic scenario, a vaccine which can induce rapid and r...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.09.013
更新日期：2013-12-26 00:00:00
abstract：:Emergent agricultural pathogens cause severe damage worldwide and their invasive potential is significantly increased by global trade, crop intensification and climate change. Standard surveillance and diagnostic protocols need to be evaluated and implemented, particularly with diseases caused by a wide range of patho...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2020.197959
更新日期：2020-08-01 00:00:00
abstract：:Retroviruses and retrotransposons package genomic RNA into virus-like particles (VLPs) in a poorly understood process. Expression of the budding yeast retrotransposon Ty3 results in the formation of cytoplasmic Ty3 VLP assembly foci comprised of Ty3 RNA and proteins, and cellular factors associated with RNA processing...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.10.008
更新日期：2013-02-01 00:00:00
abstract：:The capsid structures of the 7 serotypes of foot-and-mouth disease virus have been compared utilizing a series of neutralizing monoclonal antibodies which were previously shown to recognize at least 4 distinct epitopes on type A12 virus. A radioimmune binding assay using radioactively labeled antigens and the monoclon...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(86)90055-9
更新日期：1986-10-01 00:00:00
abstract：:A sero-epidemiologic survey of 74 IVDUs and 86 age-matched controls was performed to investigate the prevalence, clinical significance, and genetic distribution of GB virus C (GBV-C)/hepatitis G virus (HGV) infection among intravenous drug users (IVDUs) in Japan. GBV-C/HGV RNA was detected by reverse transcriptase-hem...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(97)01470-6
更新日期：1997-06-01 00:00:00
abstract：:Our understanding of picornavirus RNA replication has improved over the past 10 years, due in large part to the discovery of cis-active RNA elements (CREs) within picornavirus RNA genomes. CREs function as templates for the conversion of VPg, the Viral Protein of the genome, into VPgpUpU(OH). These so called CREs are ...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2008.07.027
更新日期：2009-02-01 00:00:00
abstract：:We have rescued a transfectant influenza virus, NA/TAIL(-), whose neuraminidase (NA) protein lacks the predicted cytoplasmic tail. The virus was attenuated (one log10 reduction) both in tissue culture and in mouse lungs. Attenuation correlated with a 50% reduction of the level of NA in infected cells and levels of inc...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(95)00017-k
更新日期：1995-06-01 00:00:00
abstract：:The varicella zoster virus (VZV) glycoprotein H (gH) stimulates VZV-specific immune responses and may be involved in virus penetration. This study reports the genomic map position and the DNA sequence of a simian varicella virus (SVV) homologue of the VZV gH gene. A 32P-labeled VZV gH-specific DNA probe hybridized to ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(95)00049-v
更新日期：1995-09-01 00:00:00
abstract：:The diagnosis of infectious diseases is sometimes difficult because of extensive immunological cross-reactivity between related viral antigens. On the path of constructing sero-specific antigens, we have identified residues involved in sero-specific and cross-reactive recognition of the nucleocapsid proteins (NPs) of ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.06.003
更新日期：2008-10-01 00:00:00
abstract：:The DNA polymerase from Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) was identified and sequenced, and its amino acid sequence was compared with other viral DNA polymerases to identify conserved regions and to reconstruct a phylogenetic tree. The sequence analysis of the AgMNPV DNA polymerase gene revealed the ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2005.01.010
更新日期：2005-06-01 00:00:00
abstract：:West Nile virus (WNV) and Japanese encephalitis virus (JEV), the members of JEV serocomplex group are pathogens of global health concern. The co-circulation of these viruses poses challenges in effective diagnostics due to antigenic similarity between the E-protein of these viruses. The present study aimed to design c...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2011.08.008
更新日期：2012-01-01 00:00:00
abstract：:The genomic RNA of vitiviruses contains 5 open reading frames (ORF). ORF3 encodes a protein to which the function of a movement protein (MP) was assigned, based on sequence homology with other viral proteins. The aim of the research described in this paper was to gain further insight in distribution profile of the ORF...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2011.10.015
更新日期：2012-01-01 00:00:00
abstract：:Mosquito-borne alphaviruses are disseminated globally and cause febrile illness in humans and animals. Since the prevalence and diversity of alphaviruses has not been previously investigated in Zambia, reverse transcription PCR was employed as a broad-spectrum approach for the detection of alphaviruses in mosquitoes. ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2018.04.005
更新日期：2018-05-02 00:00:00
abstract：:The CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9) system has been widely used for viral genome editing, transcription regulation and chromosomal localization in eukaryotic cells. In this study, a guide RNA (gRNA) that specifically recognizes HSV-1 viral genomes...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2017.03.010
更新日期：2018-01-15 00:00:00
abstract：:The identification of ligand-binding sites is often the first step in drug targeting and design. To date there are numerous computational tools available to predict ligand binding sites. These tools can guide or mitigate the need for experimental methods to identify binding sites, which often require significant resou...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.05.029
更新日期：2016-08-15 00:00:00
abstract：:The mechanism by which hepatitis B virus (HBV) infection causes severe inflammatory liver diseases is multifactorial and related to interactions with cell signaling pathways and the ensuing inflammatory response. Activation of JAK/STAT/SOCS signaling is essential for the induction of cellular antiviral responses, cont...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.12.003
更新日期：2010-03-01 00:00:00
abstract：:The hemagglutinin (HA) protein of influenza virus binds to terminal sialic acid residues present on cell surface glycoproteins and glycolipids. The specific amino acids involved in this interaction have been identified for a H3 subtype HA from the human non-pathogenic virus, A/Aichi/2/68, by both crystallographic and ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(01)00407-5
更新日期：2002-02-26 00:00:00
abstract：:The core proteins of epizootic haemorrhagic disease virus (EHDV) have important roles to perform in maintaining the structure and function of the virus. A complete genetic and phylogenetic analysis was therefore performed on these proteins (and the genes that code for them) to allow comparison of the selective pressur...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.07.011
更新日期：2009-11-01 00:00:00
abstract：:The coat protein (CP) is the only structural protein present in the polyprotein of bean common mosaic virus. The well known characteristics of the CP are self-oligomerization and nucleic acid binding activity. The studies of the coat protein mutants revealed that the oligomeric property of CP solely depends on the ami...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2019.197755
更新日期：2019-11-01 00:00:00
abstract：:Herein we describe the construction of recombinant human rhinoviruses (rHRVs) encoding HIV Gag or Tat by inserting the full length tat gene or regions of the gag gene flanked by sequences encoding the HRV 2A protease cleavage site into the junction between HRV genes encoding structural (P1) and non-structural (P2) pro...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2015.04.002
更新日期：2015-05-04 00:00:00
abstract：:A comprehensive phylogenetic study based on the hemagglutinin (H) protein of all known African measles virus (MV) isolates is presented. The study includes 64 new H gene sequences from Ghana. Nigeria and South Africa as well as viruses from Zambia and The Gambia for which only incomplete sequencing data were available...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(99)00072-6
更新日期：1999-07-01 00:00:00
abstract：:The first influenza pandemic of this century was declared in April of 2009, with the emergence of a novel H1N1 influenza A virus strain (H1N1pdm). Understanding the evolution of H1N1pdm populations within the South American region is essential for studying global diversification, emergence, resistance and vaccine effi...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.09.002
更新日期：2012-12-01 00:00:00
abstract：:Experimental infection studies with bluetongue virus (BTV) in the mammalian host have a history that stretches back to the late 18th century. Studies in a wide range of ruminant and camelid species as well as mice have been instrumental in understanding BTV transmission, bluetongue (BT) pathogenicity/pathogenesis, vir...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2013.12.044
更新日期：2014-03-01 00:00:00
abstract：:The antigenic properties of 21 Japanese field isolates and two Australian strains of Akabane (AKA) virus (Simbu serogroup, bunyavirus) isolated from 1959 to 1990 were compared by enzyme-linked immunosorbent assay (ELISA), plaque-reduction neutralization (PRNT) and hemagglutination inhibition (HI) tests using monoclona...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(96)01415-3
更新日期：1997-02-01 00:00:00
abstract：:Five premature termination mutations and five missense mutations were introduced into the portion of cloned Moloney murine leukemia virus (M-MuLV) DNA encoding the Env cytoplasmic domain. All of the mutant DNAs gave rise to replication-competent virus after transfection of NIH/3T3 cells, but several of the mutant DNAs...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(95)01278-8
更新日期：1996-03-01 00:00:00
abstract：:Nucleic acid probes prepared directly from bluetongue virus (BTV) genomic double-stranded RNA (dsRNA) have been used to identify the functionally equivalent genome segments from six distinct isolates of BTV after their separation in both agarose and polyacrylamide gel electrophoresis systems. Variations in the rate, a...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(88)90078-0
更新日期：1988-06-01 00:00:00
abstract：:Peste-des-petits-ruminants virus (PPRV) (family Paramyxoviridae, genus Morbillivirus) causes an acute febrile illness in sheep and goats resulting in significant morbidity and mortality in infected herds. The paramyxoviruses all have negative sense, non-segmented RNA genomes and their host range and pathogenic determi...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2007.01.015
更新日期：2007-06-01 00:00:00
abstract：:The 2015 Brazilian Zika virus outbreak sparked a rapid response to control the spread of the virus. What was first understood to be a mild self-resolving infection is now linked to significant neurological defects in both neonates and adults. The WHO declared the 2016 Zika epidemic a public health emergency and issued...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2017.07.025
更新日期：2018-08-02 00:00:00
abstract：:The entire genome of a mid-Atlantic raccoon strain rabies virus (RRV) isolated in Canada was sequenced; this is the second North American wildlife rabies virus isolate to be fully characterized. The overall organization and length of the genome was similar to that of other lyssaviruses. The nucleotide sequence identit...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.04.029
更新日期：2008-09-01 00:00:00
