Abstract：
:The BKRF2, BKRF3 and BKRF4 genes of Epstein-Barr virus (EBV) are located close together in the viral genome, which encode glycoprotein L, uracil-DNA glycosylase and a tegument protein, respectively. Here, we demonstrate that the BKRF2 gene behaves as a true-late lytic gene, whereas the BKRF3 and BKRF4 genes belong to the early lytic gene family. Our results further reveal that both BKRF3 and BKRF4 promoters are new synergistic targets of Zta and Rta, two EBV latent-to-lytic switch transactivators. Multiple Rta- and Zta-responsive elements within the BKRF3 and BKRF4 promoters were identified and characterized experimentally. Importantly, we show that DNA methylation is absolutely required for activation of the BKRF4 promoter by Zta alone or in combination with Rta. Moreover, we find that sodium butyrate, an inducing agent of EBV reactivation, is capable of activating the BKRF4 promoter through a mechanism independent of Zta and Rta. Overall, our studies highlight the complexity of transcriptional regulation of lytic genes within the BKRF2-BKRF3-BKRF4 gene locus.
journal_name
Virus Resjournal_title
Virus researchauthors
Chen LW,Hung CH,Wang SS,Yen JB,Liu AC,Hung YH,Chang PJdoi
10.1016/j.virusres.2018.08.005subject
Has Abstractpub_date
2018-09-02 00:00:00pages
76-89eissn
0168-1702issn
1872-7492pii
S0168-1702(18)30388-5journal_volume
256pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract：:We have used the polymerase chain reaction (PCR) to obtain sensitive detection and identification of poliovirus RNA genomes. Primer pairs were designed to permit identification of each Sabin poliovaccine strain by the electrophoretic mobilities of the amplified DNA products (Sabin 1: 97 bp; Sabin 2: 71 bp; Sabin 3: 44...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(91)90107-7
更新日期：1991-07-01 00:00:00
abstract：:Bovine enteroviruses, which belong to the Picornaviridae family, can cause clinical symptoms in cattle and are excreted in feces. In this study, a cytolytic virus was isolated from Madin-Darby bovine kidney (MDBK) cells from fecal samples of bovine with severe diarrhea and hemorrhagic intestinal mucosa that had been o...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.07.032
更新日期：2014-10-13 00:00:00
abstract：:This review compares the well-studied RNase H activities of human immunodeficiency virus, type 1 (HIV-1) and Moloney murine leukemia virus (MoMLV) reverse transcriptases. The RNase H domains of HIV-1 and MoMLV are structurally very similar, with functions assigned to conserved subregions like the RNase H primer grip a...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2007.12.007
更新日期：2008-06-01 00:00:00
abstract：:Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, is a causative agent of porcine enteric disease characterized by acute watery diarrhea and dehydration in sucking piglet. Similar to other coronaviruses, PEDV spike protein mediates its cell entry by binding to cellular receptors and induc...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.07.022
更新日期：2014-10-13 00:00:00
abstract：:The identification of ligand-binding sites is often the first step in drug targeting and design. To date there are numerous computational tools available to predict ligand binding sites. These tools can guide or mitigate the need for experimental methods to identify binding sites, which often require significant resou...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.05.029
更新日期：2016-08-15 00:00:00
abstract：:The coat protein (CP) is the only structural protein present in the polyprotein of bean common mosaic virus. The well known characteristics of the CP are self-oligomerization and nucleic acid binding activity. The studies of the coat protein mutants revealed that the oligomeric property of CP solely depends on the ami...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2019.197755
更新日期：2019-11-01 00:00:00
abstract：:Human herpesvirus 6 (HHV-6) is now classified into two distinct variants such as HHV-6 variant A(HHV-6A) and B(HHV-6B) (Ablashi et al., Arch. Virol. 129, 1993, 1-4) and the DNA of HHV-6A strain U1102 was completely sequenced (Gompels et al., Virology 209, 1995, 29-51). We have sequenced a 30-kilobase pair (kbp) (genom...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(97)00099-3
更新日期：1997-11-01 00:00:00
abstract：:Vaccines against parainfluenza (PIV) and respiratory syncytial viruses (RSV) that are currently being developed include both live and subunit vaccines. Candidate live PIV vaccines that have been found to be attenuated and efficacious in rodents or primate models are (1) cold-adapted, temperature-sensitive mutants of P...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/0168-1702(88)90063-9
更新日期：1988-08-01 00:00:00
abstract：:The human poliovirus receptor (PVR) is a cell surface protein with a multitude of functions in human biology. PVR was initially identified as the receptor for the human poliovirus and recent discoveries have given a greater insight into both its morphology and its function. Alternative splicing of the PVR gene results...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2017.09.001
更新日期：2017-10-15 00:00:00
abstract：:Conditionally replicating human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) variants that can be switched on and off at will are attractive tools for HIV and SIV research. We constructed HIV and SIV variants in which the natural transcription control mechanism was replaced by the doxycycline (...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2015.05.004
更新日期：2016-05-02 00:00:00
abstract：:The 2015 Brazilian Zika virus outbreak sparked a rapid response to control the spread of the virus. What was first understood to be a mild self-resolving infection is now linked to significant neurological defects in both neonates and adults. The WHO declared the 2016 Zika epidemic a public health emergency and issued...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2017.07.025
更新日期：2018-08-02 00:00:00
abstract：:Monocotyledonous plant infecting mastreviruses (family Geminiviridae) have been found in the Old World. The greatest diversity of these viruses has been found in Africa but this may simply reflect the more extensive sampling that has been done there. To provide a better understanding of mastrevirus diversity in Austra...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2012.07.018
更新日期：2012-10-01 00:00:00
abstract：:The compound LPRP-Et-97543 was isolated from Liriope platyphylla roots and was observed to have potential anti-viral effects in HepG2.2.15 cells against hepatitis B virus (HBV). The antiviral mode was further clarified, and the HBV-transfected Huh7 cells were used as the platform. During viral gene expression, LPRP-Et...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2014.07.015
更新日期：2014-11-04 00:00:00
abstract：:Currently, pseudorabies virus (PRV) variant strains are outbreaking in China; these variants belong to genotype II PRV. The traditional Bartha-K61 vaccine has failed to provide complete protection against the emergent variant strains. Therefore, rapid attenuation of current epidemic strains is needed for effective PRV...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.09.004
更新日期：2016-10-02 00:00:00
abstract：:We have recently identified and sequenced a molecular clone of the serogroup 2 simian retrovirus (SRV), D2/RHE/OR/V1, that retains an enhanced ability to infect specific T cell lines. In this report, using deletion mutagenesis, we localized the psi packaging signal, necessary for packaging of D2/RHE/OR/V1 particles, t...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(01)00236-2
更新日期：2001-06-01 00:00:00
abstract：:The subcellular distribution of influenza polymerase PA subunit has been studied using a SV40-recombinant virus (SVPA76), which allows the expression and accumulation of this protein in COS-1 cells. In contrast to the complete nuclear localization observed for the PA subunit several hours after influenza virus infecti...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(92)90031-4
更新日期：1992-06-01 00:00:00
abstract：:Small interfering RNAs (siRNAs) have been shown to direct sequence-specific inhibition of gene expression in mammalian cells. siRNAs are RNA duplexes of 21-23 nucleotides (nts) with approximately 2nt 3' overhangs that can induce degradation of their homologous target mRNAs without interferon responses in mammalian cel...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2004.01.015
更新日期：2004-06-01 00:00:00
abstract：:The ultrastructure of the equine morbillivirus (EMV) which was implicated in the death of one human and fourteen horses in Queensland, Australia during September 1994 and a 36 year old man from Queensland in October 1995 is described. The ultrastructure of the virus and the intracellular virus-specific structures are ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(96)01307-x
更新日期：1996-07-01 00:00:00
abstract：:The core proteins of epizootic haemorrhagic disease virus (EHDV) have important roles to perform in maintaining the structure and function of the virus. A complete genetic and phylogenetic analysis was therefore performed on these proteins (and the genes that code for them) to allow comparison of the selective pressur...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.07.011
更新日期：2009-11-01 00:00:00
abstract：:Noroviruses belong to the Caliciviridae family of single-stranded positive-sense RNA viruses. The genus Norovirus includes seven genogroups (designated GI-GVII), of which GI, GII and GIV infect humans. Human noroviruses are responsible for widespread outbreaks of acute gastroenteritis and represent one of the most com...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2016.12.018
更新日期：2017-04-15 00:00:00
abstract：:Papillomaviruses infect keratinocytes and their reproduction is tied to differentiation of the skin. The E2 protein of papillomaviruses is a multifunctional early protein that binds specifically to the viral DNA to regulate genome transcription, replication, and segregation. All of these are nuclear events that requir...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2010.02.011
更新日期：2010-06-01 00:00:00
abstract：:Adenoviruses, types 2 and 12 induce amplification of SV40 DNA sequences in cells of the SV40-transformed human newborn kidney cell line, NB-E. Similarly, integrated hepatitis B virus DNA sequences in the human hepatoma cell line, PLC/*PRF/5, and bovine papillomavirus (BPV) DNA sequences in BPV-transformed mouse cells ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(90)90079-q
更新日期：1990-08-01 00:00:00
abstract：:Detection of Chronic bee paralysis virus (CBPV) is reported for the first time in two species of ants (Camponotus vagus and Formica rufa) and in Varroa destructor. A quantitative real-time PCR (qPCR) method was used to detect and quantify CBPV in infected bees, ants and mites. A minus-strand-specific RT-PCR was used t...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2007.12.011
更新日期：2008-05-01 00:00:00
abstract：:Cool-season food legumes (faba bean, lentil, chickpea and pea) and cereals (bread and durum wheat and barley) are the most important and widely cultivated crops in West Asia and North Africa (WANA), where they are the main source of carbohydrates and protein for the majority of the population. Persistently transmitted...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2008.12.007
更新日期：2009-05-01 00:00:00
abstract：:Rhabdoviruses cause devastating diseases in aquaculture, resulting in enormous economic losses. Our previous studies indicated that imidazole arctigenin derivatives possessed antiviral activities against aquatic rhabdoviruses. Based on the data of structure-activity relationship, a new imidazole arctigenin derivative,...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2020.198019
更新日期：2020-08-01 00:00:00
abstract：:Typically plasma viremia is controlled in macaques challenged with R5 tropic SHIV162P3 during the chronic phase of infection. However, in a few infected animals it sustains for a prolonged period. Persistent plasma viremia in two such macaques led to a rapid decline of CD4+ T-cells in blood leading to the induction of...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2010.04.006
更新日期：2010-08-01 00:00:00
abstract：:Friesian calves were infected with one of three isolates of bovine viral diarrhoea virus (BVDV) and used to establish parameters for an in vitro model of BVDV-reactive T-cell responses in cattle. The study assessed virus clearance, seroconversion, maturation of lymphoproliferative responses (both during and following ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(00)00131-3
更新日期：2000-03-01 00:00:00
abstract：:The protein and glycoprotein composition of a sucrose gradient fraction from vaccinia infected cells treated with rifampicin was studied. This particulate fraction contained cytoplasmic membranes and pleomorphic membranous structures. The glycoproteins (89, 42 and 20-23 kDa, respectively) were identified as the same g...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(90)90077-o
更新日期：1990-08-01 00:00:00
abstract：:A recombinant baculovirus containing a cDNA which encodes haemagglutinin-neuraminidase (HN) of Newcastle disease virus (NDV) was constructed. Spodoptera frugiperda cells infected with this recombinant virus produced a large amount of HN glycoprotein similar to the authentic HN in size. The recombinant HN glycoprotein ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(91)90059-5
更新日期：1991-06-01 00:00:00
abstract：:Sendai virus is able to fuse with liposomes even without virus receptors. To determine the roles of envelope protein, hemagglutinin-neuraminidase (HN) and fusion (F) protein, in Sendai virus-liposome fusion, we treated the virus with proteases and examined its fusion with liposomes and the conditions of HN and F prote...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(98)00137-3
更新日期：1999-02-01 00:00:00
