当前位置： SCI文献检索 > VIRUS RESEARCH期刊下所有文献 > Recombinant influenza H7 hemagglutinin containing CFLLC minidomain in the transmembrane domain showed enhanced cross-protection in mice.

Recombinant influenza H7 hemagglutinin containing CFLLC minidomain in the transmembrane domain showed enhanced cross-protection in mice.

Abstract：

:Since February 2013, H7N9 influenza virus, causing human infections with high mortality in China, has been a potential pandemic threat. The H7N9 viruses are found to diverge into distinct genotypes as other influenza viruses; thus a vaccine that can provide sufficient cross-protection against different genotypes of H7N9 viruses is urgently needed. Our previous studies demonstrated that the HA-based structural design approach by introducing a CFLLC minidomain into transmembrane domain (TM) of H1, H5 or H9 hemagglutinin (HA) proteins by replacing with H3 subtype HA TM could enhance their cross-protection. In this study, we used Sf9 insect cell expression system to express recombinant H7 HA proteins H7-53WT, in which HA gene was derived from H7N9-53 strain, and H7-53TM containing CFLLC minidomian by replacing its TM domain with H3 HA TM. We investigated whether introduction of CFLLC minidomain into H7 HA (H7-53TM) could increase its cross-reactivity and cross-protection against different genotypes of H7N9 viruses. The results showed that the H7-53TM either with or without squalene adjuvant induced increased HI antibodies, serum IgG antibodies, and IFN-γ production to a panel of 7 H7N9 viruses in mice. Vaccinated animals with H7-53TM alone showed complete protection against challenge with heterologous H7N9-MCX strain, while H7-53WT alone showed incomplete protection (80%). Furthermore, mice vaccinated with H7-53TM HA showed less body weight loss and less pulmonary lesions and inflammation after challenge with homologous or heterologous H7N9 viruses, comparing to H7-53WT. In summary, this study presents a better subunit vaccine candidate (H7-53TM) against potential H7N9 pandemic.

journal_name

Virus Res

journal_title

Virus research

authors

Wang Y,Zhang Y,Wu J,Lin Y,Wu Z,Wei Y,Wei X,Qin J,Xue C,Liu GD,Cao Y

doi

10.1016/j.virusres.2017.09.008

subject

Has Abstract

pub_date

2017-10-15 00:00:00

pages

16-23

eissn

0168-1702

issn

1872-7492

pii

S0168-1702(17)30099-0

journal_volume

242

pub_type

杂志文章

相关文献

VIRUS RESEARCH文献大全
  • The pathogenic and vaccine strains of equine infectious anemia virus differentially induce cytokine and chemokine expression and apoptosis in macrophages.

    abstract：:The attenuated equine infectious anemia virus (EIAV) vaccine was the first attenuated lentivirus vaccine to be used in a large-scale application and has been used to successfully control the spread of equine infectious anemia (EIA) in China. To better understand the potential role of cytokines in the pathogenesis of E...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2011.06.028

    authors： Lin YZ,Cao XZ,Li L,Li L,Jiang CG,Wang XF,Ma J,Zhou JH

    更新日期：2011-09-01 00:00:00

  • Conditioned medium from persistently RSV-infected macrophages alters transcriptional profile and inflammatory response of non-infected macrophages.

    abstract：:Cells susceptible to persistent viral infections undergo important changes in their biological functions as a consequence of the expression of viral gene products that are capable of altering the gene expression profile of the host cell. Previously, we reported that persistence of the RSV genome in a mouse macrophage ...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2017.01.001

    authors： Rivera-Toledo E,Salido-Guadarrama I,Rodríguez-Dorantes M,Torres-González L,Santiago-Olivares C,Gómez B

    更新日期：2017-02-15 00:00:00

  • Differential suppression of host cell protein synthesis and mRNA levels in herpes simplex virus-infected endothelial cells.

    abstract：:Earlier studies from this laboratory have shown that infection of vascular cells with herpes simplex virus 1 or 2 (HSV-1, HSV-2) results in the differential suppression of extracellular matrix proteins including fibronectin (FN), type IV collagen, thrombospondin (TSP) and Factor VIII von Willebrand protein. The presen...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(91)90047-y

    authors： Brinker JM,Ziaie Z,Kefalides NA

    更新日期：1991-05-01 00:00:00

  • MicroRNA-4331-5p promotes FMDV replication through inhibiting interferon pathways in PK-15 cells.

    abstract：:MicroRNAs play vital roles in regulating the battle between pathogens and host cells during viral challenging. MiR-4331 aggravates transmissible gastroenteritis virus (TGEV) -induced mitochondrial damage, also suppresses transcription of TGEV gene 7 via targeting cellular CDCA7. Otherwise, miR-4331-5p affects H1N1/200...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2020.198064

    authors： Ren T,Wang Y,Chen H,Wang K,Gao X,Liu L,Zhang Y,Sun Y

    更新日期：2020-09-01 00:00:00

  • The genome sequence and transmission of an iflavirus from the brown planthopper, Nilaparvata lugens.

    abstract：:A previously unknown iflavirus has been identified in a laboratory colony of the brown planthopper, Nilaparvata lugens. The iflavirus-like sequence was first identified in contig sequences obtained from transcriptome sequencing (RNA-seq) of the brown planthopper. The complete viral genome was resequenced using the San...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2013.06.005

    authors： Murakami R,Suetsugu Y,Kobayashi T,Nakashima N

    更新日期：2013-09-01 00:00:00

  • A new variant of rabbit hemorrhagic disease virus G2-like strain isolated in China.

    abstract：:To investigate the genetic variability and evolution of rabbit hemorrhagic disease virus (RHDV) strains in China, VP60 gene sequences of eight new isolates collected from farms with RHD occurrences in China between 2009 and 2014 were analyzed, and compared with the reference sequence of the vaccine strain WF/China/200...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2016.01.018

    authors： Hu B,Fan Z,Wang F,Song Y,Wei H,Liu X,Qiu R,Xu W,Yuan W,Xue J

    更新日期：2016-04-02 00:00:00

  • Amino acid at position 95 of the matrix protein is a cytopathic determinant of rabies virus.

    abstract：:The molecular mechanism involved in cytopathogenicity of rabies virus has not been fully elucidated yet. A fixed rabies virus Nishigahara strain does not induce clear cytopathic effect (CPE) in mouse neuroblastoma (NA) cells, whereas Ni-CE strain, which was established after 100 passages of Nishigahara strain in chick...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2008.05.011

    authors： Mita T,Shimizu K,Ito N,Yamada K,Ito Y,Sugiyama M,Minamoto N

    更新日期：2008-10-01 00:00:00

  • Leader (L) of Theiler's murine encephalomyelitis virus (TMEV) is required for virus growth in a murine macrophage-like cell line.

    abstract：:Theiler's murine encephalomyelitis virus is divided into two subgroups on the basis of their different biological activities. GDVII subgroup strains cause acute and fatal encephalomyelitis in mice, while TO or DA subgroup strains cause non-fatal polioencephalomyelitis in weanling mice followed by virus persistence and...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2009.11.004

    authors： Ichinose-Asakura K,Taniura N,Himeda T,Nojiri M,Okuwa T,Ohara Y

    更新日期：2010-02-01 00:00:00

  • Prevalence of multiple subtypes of influenza A virus in Japanese wild raccoons.

    abstract：:Raccoons (Procyon lotor), which are not native to Japan, have been suspected to transmit various pathogens by frequent intrusion into agricultural and residential areas. To determine influenza A virus seropositivity in raccoons in Japan, we examined a total of 634 raccoons captured in 19 towns (A-S) from 2009 to 2012....

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2014.05.004

    authors： Yamaguchi E,Sashika M,Fujii K,Kobayashi K,Bui VN,Ogawa H,Imai K

    更新日期：2014-08-30 00:00:00

  • Avian influenza and Newcastle disease viruses from northern pintail in Japan: isolation, characterization and inter-annual comparisons during 2006-2008.

    abstract：:Since wild ducks constitute a vital element in the epizootiology of avian influenza viruses (AIVs) as well as avian paramyxoviruses (APMVs) and play a key role in the ecology and inter-species transmission of these viruses, it is crucial to elucidate the diversity and prevalence of these viruses within these bird popu...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2009.02.018

    authors： Jahangir A,Ruenphet S,Ueda S,Ueno Y,Shoham D,Shindo J,Okamura M,Nakamura M,Takehara K

    更新日期：2009-07-01 00:00:00

  • Polyomavirus large T-antigen binds the "pRb related' protein p130 through sequences in conserved region 2.

    abstract：:The transforming potential of DNA tumor viruses derives mainly from the ability of their encoded oncogene products to interact with cellular proteins. Many of these viral oncoproteins share regions of sequence similarity, designated conserved region 1 and 2, which have been implicated in complex formation with pRb, th...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/s0168-1702(96)01404-9

    authors： Desjardins P,Pilon AA,Hassell JA,Mes-Masson AM

    更新日期：1997-01-01 00:00:00

  • Effect of recombinant hybrid human interferon on replication and morphogenesis of HSV-1 in monkey cells.

    abstract：:Human recombinant alpha interferon, A/D, significantly reduced the replication and cell fusion induced by herpes simplex virus type 1 in monkey cells. Thin-section electron microscopy of interferon-treated monkey cells showed distinct assembly of nucleocapsids within the nucleus. Analysis of virus-specific proteins by...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(89)90051-8

    authors： Chatterjee S,Whitley RJ

    更新日期：1989-01-01 00:00:00

  • Porcine deltacoronavirus induces caspase-dependent apoptosis through activation of the cytochrome c-mediated intrinsic mitochondrial pathway.

    abstract：:Porcine deltacoronavirus (PDCoV), a newly discovered enteric coronavirus, is a causative agent of severe clinical diarrhea and intestinal pathological damage in piglets. As a first step toward understanding the effect of PDCoV on host cells, we elucidated mechanisms underlying the process of apoptotic cell death after...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2018.06.008

    authors： Lee YJ,Lee C

    更新日期：2018-07-15 00:00:00

  • Characterization of a panel of anti-phosphoprotein monoclonal antibodies generated against the raccoon strain of rabies virus.

    abstract：:The generation of a new panel of 32 monoclonal antibodies (MAbs) reactive with the P protein of the raccoon strain of rabies virus is described. Through a series of analyses employing competitive ELISA and immunoblotting, these MAbs were classified into eight groups, each defining an antigenic site, thereby increasing...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2010.06.014

    authors： Nadin-Davis SA,Fehlner-Gardiner C,Sheen M,Wandeler AI

    更新日期：2010-09-01 00:00:00

  • In vivo tropisms and kinetics of rat theilovirus infection in immunocompetent and immunodeficient rats.

    abstract：:Rat theilovirus (RTV) is a cardiovirus related to Theiler's murine encephalomyelitis virus. While RTV is a prevalent viral pathogen of rats used in biomedical research, the pathogenesis and characterization of RTV infections is not well understood. In the studies reported herein, we used immunohistochemistry to identi...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2011.07.014

    authors： Drake MT,Besch-Williford C,Myles MH,Davis JW,Livingston RS

    更新日期：2011-09-01 00:00:00

  • Host cell proteases: Critical determinants of coronavirus tropism and pathogenesis.

    abstract：:Coronaviruses are a large group of enveloped, single-stranded positive-sense RNA viruses that infect a wide range of avian and mammalian species, including humans. The emergence of deadly human coronaviruses, severe acute respiratory syndrome coronavirus (SARS-CoV), and Middle East respiratory syndrome coronavirus (ME...

    journal_title：Virus research

    pub_type： 杂志文章,评审

    doi：10.1016/j.virusres.2014.11.021

    authors： Millet JK,Whittaker GR

    更新日期：2015-04-16 00:00:00

  • Poliovirus Receptor: More than a simple viral receptor.

    abstract：:The human poliovirus receptor (PVR) is a cell surface protein with a multitude of functions in human biology. PVR was initially identified as the receptor for the human poliovirus and recent discoveries have given a greater insight into both its morphology and its function. Alternative splicing of the PVR gene results...

    journal_title：Virus research

    pub_type： 杂志文章,评审

    doi：10.1016/j.virusres.2017.09.001

    authors： Bowers JR,Readler JM,Sharma P,Excoffon KJDA

    更新日期：2017-10-15 00:00:00

  • Exchange of genetic sequences of long terminal repeat and the env gene by a promiscuous primate type D retrovirus.

    abstract：:Squirrel monkey retrovirus (SMRV) is a New World primate type D retrovirus. It was shown that SMRV-related sequences could be detected in another New World species, the skunk. It was further suggested that SMRV and an Old World primate type C retrovirus, baboon endogenous virus (BaEV), may have exchanged their env gen...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/s0168-1702(03)00178-3

    authors： Chiu CN,Mitra R,Chiu IM

    更新日期：2003-10-01 00:00:00

  • Analysis of genome segments from six different isolates of bluetongue virus using RNA-RNA hybridisation: a generalised coding assignment for bluetongue viruses.

    abstract：:Nucleic acid probes prepared directly from bluetongue virus (BTV) genomic double-stranded RNA (dsRNA) have been used to identify the functionally equivalent genome segments from six distinct isolates of BTV after their separation in both agarose and polyacrylamide gel electrophoresis systems. Variations in the rate, a...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(88)90078-0

    authors： Pedley S,Mohamed ME,Mertens PP

    更新日期：1988-06-01 00:00:00

  • Temporal evolutionary analysis of re-emerging recombinant GII.P16_GII.2 norovirus with acute gastroenteritis in patients from Hubei Province of China, 2017.

    abstract：:Norovirus (NoV) is a major pathogenic agent of human acute viral gastroenteritis that occurs worldwide. In March 2017, a series of acute NoV-associated gastroenteritis outbreaks occurred in Hubei Province in central China. Here, we sought to better understand the main genotypes and potential evolutionary advantages of...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2018.03.016

    authors： Li J,Zhang T,Cai K,Jiang Y,Guan X,Zhan J,Zou W,Yang Z,Xing X,Wu Y,Song Y,Yu X,Xu J

    更新日期：2018-04-02 00:00:00

  • Location of neutralizing epitopes defined by monoclonal antibodies generated against the outer capsid polypeptide, VP1, of foot-and-mouth disease virus A12.

    abstract：:The epitopes of six monoclonal antibodies generated against type A12 foot-and-mouth disease virus (FMDV) VP1 or its largest cyanogen bromide fragment (13 kd) were characterized. Five of these monoclonal antibodies neutralized viral infectivity. Solid-phase and competitive antigen binding assays using virion-derived an...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(84)90006-6

    authors： Robertson BH,Morgan DO,Moore DM

    更新日期：1984-09-01 00:00:00

  • Characterization of caprine herpesvirus 1 glycoprotein D gene and its translation product.

    abstract：:Caprine herpesvirus 1 (CpHV-1) is responsible of systemic infection in neonatal kids as well as abortion and fertility disorders in adult goats. This virus is closely related to bovine herpesvirus 1 (BoHV-1) which causes infectious bovine rhinotracheitis. Glycoprotein D (gD) mediates important functions in alphaherpes...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2005.07.009

    authors： Keuser V,Detry B,Thiry J,de Fays K,Schynts F,Pastoret PP,Vanderplasschen A,Thiry E

    更新日期：2006-02-01 00:00:00

  • Heterogeneity of the L2 gene of field isolates of bluetongue virus serotype 17 from the San Joaquin Valley of California.

    abstract：:Genome segment 2 (L2) from six field isolates of bluetongue virus (BTV) serotype 17 was sequenced by cycling sequencing after the amplification of the viral cDNA by the polymerase chain reaction. The viruses were isolated from sheep, cattle and a goat in the San Joaquin Valley of California during the years 1981 and 1...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(94)90072-8

    authors： de Mattos CA,de Mattos CC,Osburn BI,MacLachlan NJ

    更新日期：1994-01-01 00:00:00

  • Analysis and solution of false-positives when testing CVA16 sera using an antibody assay against the EV71 virus.

    abstract：:Hand, foot and mouth disease (HFMD) in humans is caused mainly by Enterovirus 71(EV71) and Coxsackievirus A16 (CVA16). EV71 is associated with severe HFMD cases but not CVA16. Use of IgM-capture enzyme-linked immunosorbent assay (ELISA) is important for the early diagnosis of EV71 infection, but cross-reactivity of th...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2013.04.015

    authors： Wang C,You A,Tian X,Zhao M,Chen Y,Lin T,Zheng J,Xiao M,Zhang Y,Kuang L,Zhou Z,Zhu B

    更新日期：2013-09-01 00:00:00

  • Sphingomyelin induces structural alteration in canine parvovirus capsid.

    abstract：:One of the essential steps in canine parvovirus (CPV) infection, the release from endosomal vesicles, is dominated by interactions between the virus capsid and the endosomal membranes. In this study, the effect of sphingomyelin and phosphatidyl serine on canine parvovirus capsid and on the phospholipase A(2) (PLA(2)) ...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2007.10.008

    authors： Pakkanen K,Karttunen J,Virtanen S,Vuento M

    更新日期：2008-03-01 00:00:00

  • The entire nucleotide sequence of the genome of human hepatitis A virus (isolate MBB).

    abstract：:Hepatitis A virus (HAV) is an important human pathogen causing hepatitis, with high incidence in developed as well as in developing countries. No vaccines are available. In order to determine the primary structure of the HAV genome, we have prepared cDNAs from viral RNA and cloned these into plasmid pBR322. These clon...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(87)90026-8

    authors： Paul AV,Tada H,von der Helm K,Wissel T,Kiehn R,Wimmer E,Deinhardt F

    更新日期：1987-08-01 00:00:00

  • Regulation of varicella zoster virus gene 27 translation in vitro by upstream sequences.

    abstract：:Northern blot analysis revealed the presence of varicella-zoster virus (VZV) gene 27 transcripts in infected cells. The Sal I-G DNA fragment, located in the unique long segment of the VZV genome and containing overlapping genes 26 and 27, was analyzed in an in vitro transcription-translation system. Translation of RNA...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(88)90015-9

    authors： Mahalingam R,Wellish M,Cabirac G,Gilden D,Vafai A

    更新日期：1988-05-01 00:00:00

  • Differential roles of the 5' untranslated regions of cucumber mosaic virus RNAs 1, 2, 3 and 4 in translational competition.

    abstract：:RNA species of plant tripartite RNA viruses show distinct translational activities in vitro when the viral RNA concentration is high. However, it is not known what causes the differential translation of virion RNAs. Using an in vitro wheat germ translation system, we investigated the translation efficiencies and compe...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/s0168-1702(00)00120-9

    authors： Kwon CS,Chung WI

    更新日期：2000-02-01 00:00:00

  • The gene encoding the late nonstructural 36K protein of vaccinia virus is essential for virus reproduction.

    abstract：:Two genetic markers--the thymidine kinase gene of herpes simplex virus, and the beta-galactosidase gene of Escherichia coli--were incorporated into the 36K protein gene (IL1 gene according to the nomenclature of the Copenhagen strain of vaccinia virus; Goebel et al., 1990) from the HindIII-P DNA fragment of the LIVP s...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(93)90027-k

    authors： Shchelkunov SN,Ryazankina OI,Gashnikov PV

    更新日期：1993-06-01 00:00:00

  • Regulatory properties of the integrated long terminal repeat of the feline immunodeficiency virus.

    abstract：:To examine the regulatory properties of feline immunodeficiency virus (FIV) long terminal repeat (LTR) integrated into host chromatin, Crandell feline kidney cells were stably transfected with the FIV LTR that directs the bacterial chloramphenicol acetyltransferase (CAT) gene. Using these cells, we examined the effect...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(96)01284-1

    authors： Ikeda Y,Kawaguchi Y,Tomonaga K,Inoshima Y,Kohmoto M,Miyazawa T,Mikami T

    更新日期：1996-04-01 00:00:00