Abstract：
:Two genetic markers--the thymidine kinase gene of herpes simplex virus, and the beta-galactosidase gene of Escherichia coli--were incorporated into the 36K protein gene (IL1 gene according to the nomenclature of the Copenhagen strain of vaccinia virus; Goebel et al., 1990) from the HindIII-P DNA fragment of the LIVP strain (variant of Lister strain) of vaccinia virus (VV). After recombination of the obtained integration plasmid pVZ64-TK with the VV genome (tk-), it was found that the resultant TK+ viruses were unstable with respect to the Lac+ phenotype. On the basis of hybridization of DNA fragments of selected clones, a scheme for the formation of hybrid viruses is proposed, and an approach to a simple phenotypical discrimination between essential and non-essential genes for VV viability is described.
journal_name
Virus Resjournal_title
Virus researchauthors
Shchelkunov SN,Ryazankina OI,Gashnikov PVdoi
10.1016/0168-1702(93)90027-ksubject
Has Abstractpub_date
1993-06-01 00:00:00pages
273-83issue
3eissn
0168-1702issn
1872-7492pii
0168-1702(93)90027-Kjournal_volume
28pub_type
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