当前位置： SCI文献检索 > VIRUS RESEARCH期刊下所有文献 > Enhanced immune responses against Japanese encephalitis virus using recombinant adenoviruses coexpressing Japanese encephalitis virus envelope and porcine interleukin-6 proteins in mice.

Enhanced immune responses against Japanese encephalitis virus using recombinant adenoviruses coexpressing Japanese encephalitis virus envelope and porcine interleukin-6 proteins in mice.

Abstract：

:Japanese encephalitis is a reproductive disorder caused by Japanese encephalitis virus (JEV) in swine. Previous studies have demonstrated that recombinant adenovirus serotype 5 (Ad5) may be a potential vaccine candidate because it can express JEV envelope epitopes and induce immune responses against JEV. Still, it will be necessary to develop an adjuvant that can enhance both humoral and cellular immune responses to the recombinant antigen delivered by non-replicating Ad5. In this study, we investigated the systemic immune responses of BALB/c mice immunized with recombinant adenovirus expressing JEV envelope epitopes in combination with porcine interleukin-6 (rAdE-IL-6).The rAdE-IL-6 immunized group had the highest titers of anti-JEV antibody as detected by an enzyme-linked immunosorbent assay (ELISA), as well as the highest levels of neutralizing antibody (1:75) as detected by a serum neutralization test. Similarly, higher concentrations of interferon-gamma (834.7pg/ml) and interleukin-6 (IL-6) (229.7pg/ml) were detected in the rAdE-IL-6 group using an ELISA assay. These data indicate that immunized BALB/c induce a strong cellular response against rAdE-IL-6. Furthermore, after challenge with the virulent JEV SCYA201201 strain, the rAdE-IL-6 group generated an immune protective response 70% greater than that of the control group, indicating that rAdE-IL-6 induced a protective immune response against JEV challenge in mice. The results from this study demonstrated that IL-6 is a strong adjuvant that can enhance both humoral and cellular immune responses in mice. Furthermore, a recombinant adenovirus coexpressing JEV envelope epitopes and porcine IL-6 protein may be an effective vaccine in animals.

journal_name

Virus Res

journal_title

Virus research

authors

Liu H,Wu R,Liu K,Yuan L,Huang X,Wen Y,Ma X,Yan Q,Zhao Q,Wen X,Cao S

doi

10.1016/j.virusres.2016.05.025

subject

Has Abstract

pub_date

2016-08-15 00:00:00

pages

34-40

eissn

0168-1702

issn

1872-7492

pii

S0168-1702(16)30061-2

journal_volume

222

pub_type

杂志文章

相关文献

VIRUS RESEARCH文献大全
  • Recombinant-baculovirus-expressed PB2 subunit of the influenza A virus RNA polymerase binds cap groups as an isolated subunit.

    abstract：:The influenza A virus RNA-dependent RNA polymerase catalyzes several reactions in transcription and replication of the genome RNA. The first step in viral mRNA synthesis is the recognition of the 5' end cap structure of host cell hnRNA and the cleavage of the RNA substrate between 10 and 14 nucleotides from the 5' end...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(96)01289-0

    authors： Shi L,Galarza JM,Summers DF

    更新日期：1996-06-01 00:00:00

  • Maturation efficiency of viral glycoproteins in the ER impacts the production of influenza A virus.

    abstract：:We have studied which steps are enhanced in the infectious cycle of influenza A virus in Madin-Darby canine kidney (MDCK) cells, a cell line investigated for use in the production of an influenza vaccine because of its ability to yield high levels of virus. We have confirmed that MDCK had the highest production levels...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2008.04.028

    authors： Ueda M,Yamate M,Du A,Daidoji T,Okuno Y,Ikuta K,Nakaya T

    更新日期：2008-09-01 00:00:00

  • Gene expression regulated by gene VI of caulimovirus: transactivation of downstream genes of transcripts by gene VI of peanut chlorotic streak virus in transgenic tobacco.

    abstract：:Here we document that the gene VI product of peanut chlorotic streak virus (PClSV), a newly characterized member of the group, transactivates the translation of dicistronic transcripts. Dicistronic expression units have been analyzed both in protoplast transient expression experiments and in transgenic tobacco plants....

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/s0168-1702(98)00088-4

    authors： Maiti IB,Richins RD,Shepherd RJ

    更新日期：1998-10-01 00:00:00

  • The capsid protein of vesicular exanthema of swine virus serotype A48: relationship to the capsid protein of other animal caliciviruses.

    abstract：:Vesicular exanthema of swine virus (VESV), the prototype calicivirus, is the etiologic agent of the porcine disease vesicular exanthema of swine (VES). VES is characterized by vesicle formation on the extremities, mouth and snout and causes abortions and stillbirths if infection occurs during pregnancy. VESV is consid...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/s0168-1702(98)00013-6

    authors： Neill JD,Meyer RF,Seal BS

    更新日期：1998-03-01 00:00:00

  • Effect of reaction conditions and 3AB on the mutation rate of poliovirus RNA-dependent RNA polymerase in a alpha-complementation assay.

    abstract：:The fidelity of poliovirus RNA-dependent RNA polymerase (3Dpol) was determined using a modified alpha-complementation assay. Several polymerases have been analyzed by this approach allowing comparisons to be drawn. Various conditions including high and low MgCl(2), replacing MgCl(2) with MnCl(2), skewed nucleotide poo...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2009.10.006

    authors： DeStefano JJ

    更新日期：2010-01-01 00:00:00

  • A review of experimental infections with bluetongue virus in the mammalian host.

    abstract：:Experimental infection studies with bluetongue virus (BTV) in the mammalian host have a history that stretches back to the late 18th century. Studies in a wide range of ruminant and camelid species as well as mice have been instrumental in understanding BTV transmission, bluetongue (BT) pathogenicity/pathogenesis, vir...

    journal_title：Virus research

    pub_type： 杂志文章,评审

    doi：10.1016/j.virusres.2013.12.044

    authors： Coetzee P,van Vuuren M,Venter EH,Stokstad M

    更新日期：2014-03-01 00:00:00

  • Leader (L) of Theiler's murine encephalomyelitis virus (TMEV) is required for virus growth in a murine macrophage-like cell line.

    abstract：:Theiler's murine encephalomyelitis virus is divided into two subgroups on the basis of their different biological activities. GDVII subgroup strains cause acute and fatal encephalomyelitis in mice, while TO or DA subgroup strains cause non-fatal polioencephalomyelitis in weanling mice followed by virus persistence and...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2009.11.004

    authors： Ichinose-Asakura K,Taniura N,Himeda T,Nojiri M,Okuwa T,Ohara Y

    更新日期：2010-02-01 00:00:00

  • Surveillance and diagnostics of the emergent Sri Lankan cassava mosaic virus (Fam. Geminiviridae) in Southeast Asia.

    abstract：:Emergent agricultural pathogens cause severe damage worldwide and their invasive potential is significantly increased by global trade, crop intensification and climate change. Standard surveillance and diagnostic protocols need to be evaluated and implemented, particularly with diseases caused by a wide range of patho...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2020.197959

    authors： Siriwan W,Jimenez J,Hemniam N,Saokham K,Lopez-Alvarez D,Leiva AM,Martinez A,Mwanzia L,Becerra Lopez-Lavalle LA,Cuellar WJ

    更新日期：2020-08-01 00:00:00

  • Avian influenza and Newcastle disease viruses from northern pintail in Japan: isolation, characterization and inter-annual comparisons during 2006-2008.

    abstract：:Since wild ducks constitute a vital element in the epizootiology of avian influenza viruses (AIVs) as well as avian paramyxoviruses (APMVs) and play a key role in the ecology and inter-species transmission of these viruses, it is crucial to elucidate the diversity and prevalence of these viruses within these bird popu...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2009.02.018

    authors： Jahangir A,Ruenphet S,Ueda S,Ueno Y,Shoham D,Shindo J,Okamura M,Nakamura M,Takehara K

    更新日期：2009-07-01 00:00:00

  • Antigenic differentiation of classical swine fever viruses in China by monoclonal antibodies.

    abstract：:The phylogenetic diversity of classical swine fever virus (CSFV) in China has been extensively studied previously, with the report of the classification of Chinese CSFVs into four subgroups within two of the established genotypes, but the antigenic differences amongst Chinese CSF viruses still remain unknown. To addre...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2009.02.011

    authors： Zhu Y,Shi Z,Drew TW,Wang Q,Qiu H,Guo H,Tu C

    更新日期：2009-06-01 00:00:00

  • Host gene expression changes in cattle infected with Alcelaphine herpesvirus 1.

    abstract：:Malignant catarrhal fever is a lymphoproliferative disease of cattle and other ungulates caused by infection with gamma-herpesviruses of the genus Macavirus. These viruses do not establish a productive infection but instead replicate in a cell-associated fashion in T lymphocytes, leading to systemic immune dysregulati...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2012.08.011

    authors： Russell GC,Benavides J,Grant DM,Todd H,Thomson J,Puri V,Nath M,Haig DM

    更新日期：2012-10-01 00:00:00

  • Trans-activation of viral tk promoters by proteins encoded by varicella zoster virus open reading frames 61 and 62.

    abstract：:Plasmids containing the varicella zoster virus (VZV) open reading frames (ORFs) 61 and 62 were used in a transient co-transfection assay to test for trans-activation of the VZV and herpes simplex virus type 1 (HSV-1) thymidine kinase (tk) promoters. The trans-activating potential of the polypeptides encoded by these V...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(90)90013-2

    authors： Cabirac GF,Mahalingam R,Wellish M,Gilden DH

    更新日期：1990-01-01 00:00:00

  • Conservation and polymorphism of EBV RPMS1 gene in EBV-associated tumors and healthy individuals from endemic and non-endemic nasopharyngeal carcinoma areas in China.

    abstract：:As a member of the BamHI-A rightward transcripts family of the Epstein-Barr virus (EBV), RPMS1 expression has been confirmed in all EBV-associated tumors. However, few studies have investigated the single-nucleotide polymorphisms (SNPs) of RPMS1, and only one SNP site (g155391a) has been reported to be associated with...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2018.04.009

    authors： Wu S,Liu W,Li H,Zhao Z,Yang Y,Xiao H,Song Y,Luo B

    更新日期：2018-05-02 00:00:00

  • Genetic susceptibility to feline infectious peritonitis in Birman cats.

    abstract：:Genetic factors are presumed to influence the incidence of feline infectious peritonitis (FIP), especially among pedigreed cats. However, proof for the existence of such factors has been limited and mainly anecdotal. Therefore, we sought evidence for genetic susceptibility to FIP using feline high density single nucle...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2013.04.006

    authors： Golovko L,Lyons LA,Liu H,Sørensen A,Wehnert S,Pedersen NC

    更新日期：2013-07-01 00:00:00

  • Recombination analyses between two strains of porcine reproductive and respiratory syndrome virus in vivo.

    abstract：:Porcine reproductive and respiratory syndrome virus (PRRSV) is characteristic of genetically extensive variation. In this study, five SPF pigs were co-infected with two strains of PRRSV (JXwn06-81c and HB-1/3.9c), and 352 viruses were cloned by plaque assay from the sera of the infected pigs on days 3, 5, 7, 10, 14, 2...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2010.12.003

    authors： Liu D,Zhou R,Zhang J,Zhou L,Jiang Q,Guo X,Ge X,Yang H

    更新日期：2011-02-01 00:00:00

  • Reverse genetics for peste-des-petits-ruminants virus (PPRV): promoter and protein specificities.

    abstract：:Peste-des-petits-ruminants virus (PPRV) (family Paramyxoviridae, genus Morbillivirus) causes an acute febrile illness in sheep and goats resulting in significant morbidity and mortality in infected herds. The paramyxoviruses all have negative sense, non-segmented RNA genomes and their host range and pathogenic determi...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2007.01.015

    authors： Bailey D,Chard LS,Dash P,Barrett T,Banyard AC

    更新日期：2007-06-01 00:00:00

  • Recombinant equine herpesvirus 1 (EHV-1) vaccine protects pigs against challenge with influenza A(H1N1)pmd09.

    abstract：:Swine influenza virus (SIV) is not only an important respiratory pathogen in pigs but also a threat to human health. The pandemic influenza A(H1N1)pdm09 virus likely originated in swine through reassortment between a North American triple reassortant and Eurasian avian-like SIV. The North American triple reassortant v...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2013.01.004

    authors： Said A,Lange E,Beer M,Damiani A,Osterrieder N

    更新日期：2013-05-01 00:00:00

  • Phylogenetic analysis of the RNA-dependent RNA polymerase (RdRp) and a predicted structural protein (pSP) of the Chronic bee paralysis virus (CBPV) isolated from various geographic regions.

    abstract：:Chronic bee paralysis virus (CBPV) is responsible for chronic paralysis, an infectious and contagious disease of adult honey bees (Apis mellifera L.). The full-length nucleotide sequences of the two major RNAs of CBPV have previously been characterized. The Orf3 of RNA1 has shown significant similarities to the RNA-de...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2009.04.025

    authors： Blanchard P,Schurr F,Olivier V,Celle O,Antùnez K,Bakonyi T,Berthoud H,Haubruge E,Higes M,Kasprzak S,Koeglberger H,Kryger P,Thiéry R,Ribière M

    更新日期：2009-09-01 00:00:00

  • Detection of intersubtype recombinants with respect to env and nef genes of HIV-1 among female sex workers in Calcutta, India.

    abstract：:HIV-1 detected among female sex workers in Calcutta, India was characterized in respect to env and nef genes. A total of 39 HIV-1 seropositive samples were used in the study. Phylogenetic analysis of the nucleotide sequences of respective regions showed that 22 out of 39 samples (56.4%) were infected with subtype C wi...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2007.06.013

    authors： Bhanja P,Sengupta S,Banerjee D,Sarkar K,Jana S,Chakrabarti S

    更新日期：2007-12-01 00:00:00

  • Comparative full genome analysis revealed E1: A226V shift in 2007 Indian Chikungunya virus isolates.

    abstract：:The resurgence of Chikungunya virus (CHIKV) in the form of unprecedented explosive epidemic after a gap of 32 years in India is a point of major public health concern. In 2007 again there was outbreak in Kerala, India, affecting more than 25,000 cases with many reported mortalities. To understand the molecular basis o...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2008.02.004

    authors： Santhosh SR,Dash PK,Parida MM,Khan M,Tiwari M,Lakshmana Rao PV

    更新日期：2008-07-01 00:00:00

  • Cervical and anal HPV infections in HIV positive women and men.

    abstract：:The goal of this review is to summarize recently published epidemiological information that contribute to understanding the natural history of cervical and human papillomavirus (HPV) infection and their associated lesions among human immunodeficiency virus (HIV) infected women and men. HIV-positive women and men are m...

    journal_title：Virus research

    pub_type： 杂志文章,评审

    doi：10.1016/s0168-1702(02)00189-2

    authors： de Sanjosé S,Palefsky J

    更新日期：2002-11-01 00:00:00

  • Correlation of cellular factors and differential scrapie prion permissiveness in ovine microglia.

    abstract：:Prion diseases are fatal neurodegenerative disorders by which the native cellular prion protein (PrPC) is misfolded into an accumulating, disease-associated isoform (PrPD). To improve the understanding of prion pathogenesis and develop effective treatments, it is essential to elucidate factors contributing to cellular...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2017.07.016

    authors： Dinkel KD,Schneider DA,Muñoz-Gutiérrez JF,McElliott VR,Stanton JB

    更新日期：2017-08-15 00:00:00

  • Two evolutionary strategies of influenza viruses to escape host non-specific inhibitors: alteration of hemagglutinin or neuraminidase specificity.

    abstract：:The porcine serum inhibitor alpha 2-macroglobulin prevents influenza virus from entering host cells by competing for the SA alpha 2, 6Gal-binding site of the hemagglutinin (HA). We studied a series of inhibitor-sensitive and inhibitor-resistant human and porcine influenza virus isolates of the H3N2 subtype, all of whi...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(96)01304-4

    authors： Gimsa U,Grötzinger I,Gimsa J

    更新日期：1996-06-01 00:00:00

  • Impaired cardiac microvascular endothelial cells function induced by Coxsackievirus B3 infection and its potential role in cardiac fibrosis.

    abstract：:CVB3 virus tropism and tissue access are modulated by cardiac microvascular endothelial cells (CMVECs) in the context of microvasculature. This study was designed to examine biological behaviors of CMVECs following CVB3 infection and its possible effects on cardiac remodeling. Data demonstrated that CVB3 increased cas...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2012.07.027

    authors： Xie Y,Liao J,Li M,Wang X,Yang Y,Ge J,Chen R,Chen H

    更新日期：2012-10-01 00:00:00

  • Detection and identification of vaccine-related polioviruses by the polymerase chain reaction.

    abstract：:We have used the polymerase chain reaction (PCR) to obtain sensitive detection and identification of poliovirus RNA genomes. Primer pairs were designed to permit identification of each Sabin poliovaccine strain by the electrophoretic mobilities of the amplified DNA products (Sabin 1: 97 bp; Sabin 2: 71 bp; Sabin 3: 44...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(91)90107-7

    authors： Yang CF,De L,Holloway BP,Pallansch MA,Kew OM

    更新日期：1991-07-01 00:00:00

  • Encephalomyocarditis virus is potentially derived from eastern bent-wing bats living in East Asian countries.

    abstract：:Bats are reservoir hosts of many zoonotic viruses and identification of viruses that they carry is important. This study aimed to use high throughput screening to identify the viruses in fecal guano of Taiwanese insectivorous bats caves in order to obtain more information on bat-derived pathogenic viruses in East Asia...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2018.10.020

    authors： Doysabas KCC,Oba M,Furuta M,Iida K,Omatsu T,Furuya T,Okada T,Sutummaporn K,Shimoda H,Wong ML,Wu CH,Ohmori Y,Kobayashi R,Hengjan Y,Yonemitsu K,Kuwata R,Kim YK,Han SH,Sohn JH,Han SH,Suzuki K,Kimura J,Maeda K,O

    更新日期：2019-01-02 00:00:00

  • Proteomic analysis of the host response in the bursa of Fabricius of chickens infected with Marek's disease virus.

    abstract：:Marek's disease virus (MDV) is an oncogenic herpesvirus that causes a disease in chickens characterized by tumor formation and immunosuppression. In this paper, we identified differentially expressed proteins in the bursa of Fabricius of chickens infected with the highly virulent MDV strain RB1B, using two-dimensional...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/j.virusres.2010.08.010

    authors： Lu Z,Qin A,Qian K,Chen X,Jin W,Zhu Y,Eltahir YM

    更新日期：2010-11-01 00:00:00

  • The pathogenesis of soluble PrP fragments containing Aβ binding sites.

    abstract：:Prion protein (PrP) has proven to bind amyloid beta (Aβ) oligomers with high affinity, changing our understanding of both prion diseases (PD) and Alzheimer's disease (AD) at the molecular and phenotypic levels, although the latter currently lacks sufficient attentions. Transgenic mice expressing anchorless PrP develop...

    journal_title：Virus research

    pub_type： 杂志文章,评审

    doi：10.1016/j.virusres.2015.10.023

    authors： Li B

    更新日期：2016-01-04 00:00:00

  • Reduced virulence of a Junin virus mutant is associated with restricted multiplication in murine cells.

    abstract：:C167, a mutant derived from the XJC13 strain of Junin virus, is highly attenuated in its pathogenic properties for newborn mice. Whereas 10(2).PFU of XJC13 injected intracerebrally killed 100% of two-day-old mice, the mutant showed no detectable lethality. Survival of mice infected with C167 was associated with a redu...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(89)90074-9

    authors： Scolaro LA,Mersich SE,Damonte EB

    更新日期：1989-08-01 00:00:00

  • Characterisation of Wongorr virus, an Australian orbivirus.

    abstract：:Sequence analyses of VP3 gene segments of Wongorr virus isolates from the Northern Territory of Australia were compared with the cognate gene segments from Picola and Paroo River viruses. Previous serological investigations had demonstrated some relationships between these viruses, however VP3 gene sequence and phylog...

    journal_title：Virus research

    pub_type： 杂志文章

    doi：10.1016/0168-1702(96)01344-5

    authors： Parkes H,Gould AR

    更新日期：1996-10-01 00:00:00