Abstract:
:The nucleocapsids formation is a pivotal step of hepatitis B virus (HBV) life cycle. The inhibition of HBV nucleocapsids assembly is a promising strategy for the anti-HBV treatment. HBc78-117 is an internal fragment of hepatitis B core protein (HBc). In this study, we used lentiviral vector to deliver HBc78-117 cDNA sequence into HepG2.2.15 cells and examined the effect of HBc78-117 on HBV replication. We confirmed by immunoprecipitation analysis that HBc78-117 interacted with full-length HBc in HepG2.2.15 cells. The nucleocapsids and HBV DNA replication intermediates were markedly reduced in the cells expressing HBc78-117, although HBV pregenome RNA was not affected. The level of HBV DNA was also significantly reduced in culture supernatant. These suggest that HBc78-117 can inhibit HBV DNA replication by interfering with nucleocapsids assembly.
journal_name
Virus Resjournal_title
Virus researchauthors
Han J,Pan X,Gao Y,Weil Ldoi
10.1016/j.virusres.2010.03.005subject
Has Abstractpub_date
2010-06-01 00:00:00pages
129-34issue
1-2eissn
0168-1702issn
1872-7492pii
S0168-1702(10)00105-Xjournal_volume
150pub_type
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