Abstract：
:To model HCV resistance to a treatment with interferon-alpha (IFN-alpha) and ribavirin, Huh7 cells, bearing HCV subgenomic replicons, were treated with these compounds for several weeks. Analysis of the cell clones, which were able to support replication of HCV RNA in the presence of high concentrations of these antivirals, demonstrated that the observed resistance was due to changes in the host cell phenotype but not to the emergence of resistant variants of the replicon. No changes in the type I IFN receptor mRNA levels or sequences were found in IFN-treated cells suggesting that the observed resistance of replicon-containing cells to IFN-alpha was caused by modifications of some other cellular factors. The resistance of cells to high concentrations of ribavirin was due to a single point mutation in the NS5A gene of the HCV replicon, and was not associated with a defect in a ribavirin uptake. This mutation, however, did not change the sensitivity of the replicon itself to this antiviral.
journal_name
Virus Resjournal_title
Virus researchauthors
Aus dem Siepen M,Oniangue-Ndza C,Wiese M,Ross S,Roggendorf M,Viazov Sdoi
10.1016/j.virusres.2006.12.007subject
Has Abstractpub_date
2007-04-01 00:00:00pages
109-13issue
1eissn
0168-1702issn
1872-7492pii
S0168-1702(06)00372-8journal_volume
125pub_type
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