Abstract:
:The compound LPRP-Et-97543 was isolated from Liriope platyphylla roots and was observed to have potential anti-viral effects in HepG2.2.15 cells against hepatitis B virus (HBV). The antiviral mode was further clarified, and the HBV-transfected Huh7 cells were used as the platform. During viral gene expression, LPRP-Et-97543 treatment had apparent effects on the viral precore/pregenomic and S/preS RNA. Promoter activity analysis demonstrated that LPRP-Et-97543 significantly reduced Core, S, and preS but not X promoter activities. Further examination showed that putative signaling pathways were involved in this inhibitory effect, indicating that NF-κB may serve a putative mediator of HBV gene regulation with LPRP-Et-97543. In addition, the nuclear expression of p65/p50 NF-κB member proteins was attenuated with LPRP-Et-97543 and augmented cytoplasmic IκBα protein levels but without affecting the expression of these proteins in HBV non-transfected cells during treatment. Moreover, LPRP-Et-97543 reduced the binding activity of NF-κB protein to CS1 element of HBV surface gene in a gel retardation analysis and inhibited CS1 containing promoter activity in HBV expressed cells. However, HBV transfection significantly enhances CS1 containing promoter activity without compound treatment in cells. Finally, transfection of the p65 expression plasmid significantly reversed the inhibitory effect of LPRP-Et-97543 on the replicated HBV DNA level in HBV positive cells. In conclusion, this study suggests that the mechanism of HBV inhibition by LPRP-Et-97543 may involve the feedback regulation of viral gene expression and viral DNA replication by HBV viral proteins, which interferes with the NF-κB signaling pathway.
journal_name
Virus Resjournal_title
Virus researchauthors
Huang TJ,Tsai YC,Chiang SY,Wang GJ,Kuo YC,Chang YC,Wu YY,Wu YCdoi
10.1016/j.virusres.2014.07.015subject
Has Abstractpub_date
2014-11-04 00:00:00pages
16-24eissn
0168-1702issn
1872-7492pii
S0168-1702(14)00281-0journal_volume
192pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract::The K1L protein of vaccinia virus is required for its growth in certain cell lines (RK-13 and human). The cowpox host-range protein CP77 has been shown to complement K1L function in RK-13 cells, despite a lack of homology between the two proteins except for ankyrin repeats. We investigated the role of ankyrin repeats ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2005.06.003
更新日期:2005-12-01 00:00:00
abstract::Eighteen monoclonal antibodies were produced against three Australian bovine enterovirus serogroups. Based on their binding patterns to 14S subunits, natural empty capsids and virions of 4 selected viral isolates (K2577, SL305, 66/27, J2129) in two types of quantitative ELISAs, the 18 antibodies were shown to belong t...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(90)90050-l
更新日期:1990-07-01 00:00:00
abstract::Meliacine (MA), an antiviral principle present in partially purified leaf extracts of Melia azedarach L., prevents the development of herpetic stromal keratitis (HSK) in mice by diminishing the viral load in the eye and the severity of lesions caused by a virus-induced immunopathological reaction. The tetranortriterpe...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2008.12.013
更新日期:2009-04-01 00:00:00
abstract::The T-helper response to the measles virus nucleoprotein (NP) has been studied in mice. The T-cell proliferative response was measured in lymphocytes from mice immunized with either a vaccinia measles-NP recombinant virus or a mouse neuro-adapted measles virus. A T-cell response was obtained with lymphocytes from H2d ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(92)90088-q
更新日期:1992-01-01 00:00:00
abstract::We describe a rapid method for genetic characterisation of influenza virus genes using reverse transcription and amplification by polymerase chain reaction (RT/PCR) of all virus segments simultaneously (multiplex RT/PCR) using primers based on the conserved terminal sequences. The product has been shown to be suitable...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(94)90087-6
更新日期:1994-06-01 00:00:00
abstract::Human torovirus (HTV) and Breda virus (BRV), members of the genus torovirus in the family Coronaviridae, are established infectious agents of humans and cattle, respectively. The hemagglutinin-esterase (HE) gene of Breda virus serotype 2 (BRV-2) has been identified and the nucleotide sequence for BRV serotype 1 (BRV-1...
journal_title:Virus research
pub_type: 杂志文章,收录出版
doi:10.1016/s0168-1702(99)00088-x
更新日期:1999-11-01 00:00:00
abstract::The nucleotide sequence of the matrix gene (M) of a recently identified morbillivirus, phocid distemper virus (PDV), was determined and the amino acid composition deduced. The M gene of PDV shared many characteristics with the corresponding gene in other morbilliviruses. The nucleotide homology with the closely relate...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(92)90064-g
更新日期:1992-04-01 00:00:00
abstract::The complete nucleotide sequence (2209 nucleotides) of the RNA 3 of the cucumber mosaic virus strain Trk7 was determined. Trk7-CMV possesses two open reading frames which encode the 3a protein (279 amino acids) and the coat protein (218 amino acids). Sequence analysis proved the earlier biological and serological clas...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(94)90030-2
更新日期:1994-03-01 00:00:00
abstract::In recent years, the spread of Duck Hepatitis A Viruses (DHAVs) has represented a serious threat and significant economic impact in duck industry of China. The sixteen reported DHAV isolates (15 DHAV-1 strains and one DHAV-3) were identified from infected ducks with clinical symptoms in China between 2009 and 2012. In...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2013.10.007
更新日期:2013-12-26 00:00:00
abstract::Variants assigned to GI-23 lineage of infectious bronchitis virus (IBV), formerly called Var2, have circulated for nearly 20 years only in countries of the Middle East. Strains of this lineage were first identified in Israel in 1998. More severe form of the virus appeared in 2006, when the second wave of Var2 epidemic...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2017.09.010
更新日期:2017-10-15 00:00:00
abstract::Transposable elements are valuable for somatic and germ-line transformation. However, long interspersed nuclear elements (LINEs) have not been used because of poor information on the transposition mechanism. We have developed a novel gene delivery system combining baculovirus AcNPV and two silkworm LINEs, SART1 and R1...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2007.03.014
更新日期:2007-07-01 00:00:00
abstract::The sub-cellular location and mechanism of membrane association of NS3 and NS2-3 polypeptides of bovine viral diarrhoea virus (BVDV) have been examined. Both NS3 and NS2-3 proteins were detected in post-nuclear membrane fractions but not in cytosolic fractions of BVDV infected cells; a proportion of NS3, but not NS2-3...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(03)00223-5
更新日期:2003-11-01 00:00:00
abstract::African swine fever virus (ASFV) is the etiological agent of a contagious and often lethal disease of domestic pigs that has significant economic consequences for the swine industry. The viral genome encodes for more than 150 genes, and only a select few of these genes have been studied in some detail. Here we report ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2016.07.013
更新日期:2016-09-02 00:00:00
abstract::HCV is an ideal model to study how the infected cell is altered to allow the establishment of a chronic infection. After infection, the transcriptome of the cell changes in response to the virus or to the antiviral pathways induced by infection. The cell has evolved to sense HCV soon after infection and to activate an...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2015.10.001
更新日期:2016-01-02 00:00:00
abstract::A molecular epidemiological study was performed on 13 Korean virus isolates, which were collected from wild and domestic animals diagnosed as rabid between 1998 and 2004. Seven samples were from domestic animals such as dogs and cattle infected by rabid raccoon dogs (Nyctereutes procyonoides koreensis), and the rest o...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2005.06.004
更新日期:2005-12-01 00:00:00
abstract::As a member of the BamHI-A rightward transcripts family of the Epstein-Barr virus (EBV), RPMS1 expression has been confirmed in all EBV-associated tumors. However, few studies have investigated the single-nucleotide polymorphisms (SNPs) of RPMS1, and only one SNP site (g155391a) has been reported to be associated with...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2018.04.009
更新日期:2018-05-02 00:00:00
abstract::The measles virus induces a life-long immune response associated with antibodies specific for the fusion protein. To map the linear immunodominant recognition sites of the fusion (F) protein of the measles virus, we have reacted a complete set of 108 overlapping pentadecapeptides with purified IgG obtained from donor ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(93)90095-5
更新日期:1993-12-01 00:00:00
abstract::Porcine enteric coronaviruses (CoVs) cause severe disease in the porcine herds worldwide, leading to important economic losses. Despite the knowledge of these viruses since the 1970s, vaccination strategies have not been implemented, leading to continuous re-emergence of novel virulent strains. Live attenuated vaccine...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2016.07.003
更新日期:2016-12-02 00:00:00
abstract::Transmissible spongiform encephalopathies (TSE) are a heterogeneous group of neurodegenerative disorders. The common feature of these diseases is the pathological conversion of the normal cellular prion protein (PrP(C)) into a β-structure-rich conformer-termed PrP(Sc). The latter can induce a self-perpetuating process...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2014.10.020
更新日期:2015-09-02 00:00:00
abstract::The inner capsid protein VP6 of group A rotavirus possesses group and subgroup epitope specificities. Avian rotaviruses have a unique VP6 that is antigenically different from its mammalian counterpart. The lack of information on the VP6 protein of chicken rotavirus strain, CH2, at the genetic and antigenic level was a...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2008.07.008
更新日期:2008-11-01 00:00:00
abstract::The A antigen gene from a very virulent strain of Marek's disease virus, RB-1B, has been cloned and the nucleotide sequence determined. The predicted amino acid sequence showed 99% identity to that determined for the MDV GA A antigen (Coussens and Velicer, J. Virol. 62, 2373-2379, 1988) over all but the carboxy-termin...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(89)90094-4
更新日期:1989-04-01 00:00:00
abstract::Norovirus (NoV) is a major pathogenic agent of human acute viral gastroenteritis that occurs worldwide. In March 2017, a series of acute NoV-associated gastroenteritis outbreaks occurred in Hubei Province in central China. Here, we sought to better understand the main genotypes and potential evolutionary advantages of...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2018.03.016
更新日期:2018-04-02 00:00:00
abstract::Expression of the rotavirus enterotoxin (NSP4) in transfected monkey kidney cells was found to result in a dramatic re-modeling of the microtubule (MT) network. This important centrosome organized cytoskeletal element was dissolved by expression of NSP4 and re-formed in a ring array at the periphery of the cell, simil...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2011.10.011
更新日期:2012-01-01 00:00:00
abstract::Five premature termination mutations and five missense mutations were introduced into the portion of cloned Moloney murine leukemia virus (M-MuLV) DNA encoding the Env cytoplasmic domain. All of the mutant DNAs gave rise to replication-competent virus after transfection of NIH/3T3 cells, but several of the mutant DNAs...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(95)01278-8
更新日期:1996-03-01 00:00:00
abstract::DNA polymerase (DNApol) is highly conserved in baculovirus and is required for viral DNA replication. However, little is known about gammabaculovirus DNApol. Here DNApol of the gammabaculovirus Neodiprion lecontei nucleopolyhedrovirus (NeleNPV) was cloned into a dnapol-null alphabaculovirus AcMNPV bacmid, creating Bac...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2019.04.005
更新日期:2019-06-01 00:00:00
abstract::We have developed a system for the preparation of La Crosse virus (LAC) and Hantaan virus (HTN) pseudotypes using a murine leukemia virus vector. After concentration, the pseudotypes were present in quantities sufficient to analyze cell tropism and neutralization. Cells resistant to LAC could not be infected with the ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(99)00070-2
更新日期:1999-10-01 00:00:00
abstract::Virus entry into host cells relies on interactions between viral and host structures including lipids, carbohydrates and proteins. Particularly, protein-protein interactions between viral surface proteins and host proteins as well as secondary host protein-protein interactions play a pivotal role in coordinating virus...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2015.09.006
更新日期:2016-06-15 00:00:00
abstract::Phylogenetic analysis has been extensively used as a tool for the reconstruction of epidemiological relations for research or for forensic purposes. It was our objective to assess the sensitivity of different phylogenetic methods and various phylogenetic programs to reconstruct epidemiological links among HIV-1 infect...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.03.002
更新日期:2012-06-01 00:00:00
abstract::SARS-CoV 3a protein was a unique protein of SARS coronavirus (SARS-CoV), which was identified in SARS-CoV infected cells and SARS patients' specimen. Recent studies revealed that 3a could interact specifically with many SARS-CoV structural proteins, such as M, E and S protein. Expressed 3a protein was reported to loca...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2005.01.001
更新日期:2005-05-01 00:00:00
abstract::Marek's disease virus (MDV) is an oncogenic herpesvirus that causes a disease in chickens characterized by tumor formation and immunosuppression. In this paper, we identified differentially expressed proteins in the bursa of Fabricius of chickens infected with the highly virulent MDV strain RB1B, using two-dimensional...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2010.08.010
更新日期:2010-11-01 00:00:00