Abstract:
:In this study, recombinant human interleukin-2 (rhIL-2) containing poly(lactic-co-glycolic acid) (PLGA) microparticles were prepared for pulmonary administration by modified w/o/w double emulsion solvent extraction method and the effects of various formulation parameters on the physicochemical properties of the microparticles were investigated. Microparticles in suitable size for pulmonary administration (4.02 µm) were obtained by increasing dichloromethane volume used in the organic phase. Also, a very high encapsulation efficiency (99.22%) value could be reached in these microparticles. In the sodium dodecyl sulphate-polyacrylamide gel electrophoresis analysis, rhIL-2 extracted from microparticles having a similar band with native rhIL-2 showed that the protein was not affected by the encapsulation process. The release curves of microparticles exhibited a biphasic fashion, characterized by a fast release phase at initial 1 day, followed by a slower one on the remaining days. Bioactivity investigations using T cells show that rhIL-2 encapsulated in PLGA microparticles retain their biological activity.
journal_name
J Microencapsuljournal_title
Journal of microencapsulationauthors
Devrim B,Bozkir A,Canefe Kdoi
10.3109/02652048.2011.599438subject
Has Abstractpub_date
2011-01-01 00:00:00pages
582-94issue
6eissn
0265-2048issn
1464-5246journal_volume
28pub_type
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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doi:10.3109/02652049409040444
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.3109/02652049009040467
更新日期:1990-10-01 00:00:00
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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doi:10.3109/02652048.2012.692397
更新日期:2012-01-01 00:00:00
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journal_title:Journal of microencapsulation
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更新日期:2004-03-01 00:00:00
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
pub_type: 杂志文章
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journal_title:Journal of microencapsulation
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abstract::To overcome the recurrence problem in bladder tumours; nanoparticles with positive surface charge may improve interaction with biological membranes for intravesical administration. The aim of this study was to design, develop and evaluate (in vitro-in vivo) cationic nanoparticles based on chitosan, poly-L-lysine or po...
journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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abstract::The chitosan microspheres were prepared by a membrane emulsification method with variations of the N2 gas pressure and the chitosan concentration. The pressure of N2 gas was varied within the range from 0.2 x 10(5) to 0.8 x 10(5) Pa at chitosan concentration 1.5 wt%. In addition, the concentration of chitosan was vari...
journal_title:Journal of microencapsulation
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journal_title:Journal of microencapsulation
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更新日期:2008-12-01 00:00:00
abstract::Poly-D,L-lactide (PDLLA) and polylactide-co-glycolide (PLGA) microspheres containing thymopentin have been prepared by a water-in-oil-in-water-emulsion/solvent evaporation technique. The goal is to stabilize the active compound thymopentin, and to prolong its therapeutic activity, by embedding the drug in a polymeric ...
journal_title:Journal of microencapsulation
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abstract::The size dependent and size independent contributions to the mechanical properties of poly(ethyleneglycol) microspheres with encapsulated air, made for medical ultrasound imaging, have been studied. A relation between the size of microspheres in a reference group and their acoustic attenuation efficacy was derived by ...
journal_title:Journal of microencapsulation
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更新日期:2000-05-01 00:00:00