Abstract:
:Transplantation of cells genetically modified to produce neurotrophins is a promising spinal cord repair strategy. Previously we showed that fibroblasts engineered to produce brain-derived neurotrophic factor (Fb/BDNF) microencapsulated in alginate survive, continue to grow and express bioactive BDNF. We here compared the effects of different alginate crosslinkers on dorsal root ganglia (DRG) neurite growth using alginate-encapsulated Fb/BDNF. Aqueous sodium alginate (±Fb/BDNF) was contacted with different calcium salts, and used as substrate for DRG growth. Length, number and orientation of neurites were measured. Chloride or carbonate salts promoted significantly more neurite growth than sulphate, with or without Fb/BDNF, although encapsulated Fb/BDNF stimulated significantly more neurite growth than cell-free. An Fb/BDNF-derived neurotrophin concentration gradient directionally guided DRG neurite growth. This positive effect of alginate-encapsulated Fb/BDNF on neurite growth/guidance shows promise for enhanced regeneration and guidance of axons towards a specific target in the injured spinal cord.
journal_name
J Microencapsuljournal_title
Journal of microencapsulationauthors
Francis NL,Shanbhag MS,Fischer I,Wheatley MAdoi
10.3109/02652048.2011.569765subject
Has Abstractpub_date
2011-01-01 00:00:00pages
353-62issue
5eissn
0265-2048issn
1464-5246journal_volume
28pub_type
杂志文章abstract::Radioembolization is used in diagnostic imaging of the lungs and for radioembolization therapy of hepatic tumours. Presently, 99mTc labelled macroaggregates or microspheres of human serum albumin (HAM) are used for this purpose. Poly lactic acid (PLA) is biodegradable, like HAM, and, unlike HAM, is not a blood product...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.1080/026520400405750
更新日期:2000-07-01 00:00:00
abstract:CONTEXT:Solid lipid nanoparticles (SLNs) can efficiently and efficaciously incorporate anti-cancer agents. OBJECTIVE:To prepare and characterise tamoxifen (TAM)-loaded SLNs. MATERIALS AND METHODS:Glyceryl monostearate, Tween-80, and trehalose were used in SLNs. SLNs were tested via dynamic light scattering (DLS), tra...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.3109/02652048.2014.898707
更新日期:2014-01-01 00:00:00
abstract::Polylactic acid microcapsules containing randomly distributed hydrocortisone particles were prepared. The rate of release of hydrocortisone from the microcapsules in pH 7.4 phosphate buffer was found to be largely increased by the presence of polysorbate 80, cetylpyridinium chloride, or aerosol OT in the dissolution m...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.3109/02652048809036721
更新日期:1988-01-01 00:00:00
abstract::The effect of lecithin and starch on viability of alginate encapsulated probiotics was determined at different temperatures. Probiotic organisms (1% v/v>10Log CFU ml(-1)) were encapsulated using alginate (2% w/v), gelatinized starches (2% w/v) and lecithin (0-4% w/v) and stored in sealed containers at 4, 23 and 37 deg...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.3109/02652040902982183
更新日期:2010-01-01 00:00:00
abstract::Different classes of excipients with potential for use in the design of novel pelletized formulations manufactured by extrusion-spheronization were examined using factorial experiments. Among the various silicates examined in a model mix with microcrystalline cellulose and lactose wetted with water, kaolin, talc and V...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.3109/02652049709006817
更新日期:1997-09-01 00:00:00
abstract::The objective of this study was selection of most influential variable for the preparation of gastro adhesive nanoparticles of acyclovir. Effect of formulation and processing variables on various response variables were studied by a Taguchi standard orthogonal array L8 design. Independent variables studied were the am...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.3109/02652048.2013.863398
更新日期:2014-01-01 00:00:00
abstract::The physical entrapment of a hydrophilic drug within degradable microspheres is generally difficult because of poor entrapment yield and/or fast release, depending on the microsphere fabrication method. In order to counter the effects of drug hydrophilicity, it is proposed to covalently attach the drug to lactic acid ...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.1080/026520400417667
更新日期:2000-09-01 00:00:00
abstract::Gelatin/gum arabic (GA) microcapsules were prepared by a convenient one step complex coacervation, in which TiO2 nanoparticles modified by stearic acid (SA) and homodispersed in tetrachloroethylene (TCE) were encapsulated. The modified TiO2 was characterized by Fourier transform infrared spectroscopy (FT-IR), dynamic ...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.1080/02652040802127806
更新日期:2009-02-01 00:00:00
abstract::Neutral phosphatidylcholine/cholesterol (10 : 3) liposomes with the reovirus M cell attachment protein sigma 1 were made by means of detergent dialysis method. An immunological evaluation method revealed an incorporation efficiency (pg protein/microgram lipid) of 314. Binding studies with mouse fibroblasts (L929 cells...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.3109/02652049009021848
更新日期:1990-07-01 00:00:00
abstract::The real issue in the development of oral controlled release dosage forms is not just to prolong the delivery of drugs but also to prolong the presence of dosage forms in the stomach in order to improve the bioavailability of drugs with a 'narrow absorption window'. In the present study, an anti-ulcer drug, ranitidine...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.1080/02652040801973101
更新日期:2008-08-01 00:00:00
abstract::This study demonstrates the feasibility of using a single-capillary electrospray (ES) system to generate novel alpha-lipoic acid encapsulated poly(ethylene oxide)-chitosan (ALA/PEO/CS) particles with a monodispersed diameter. Scanning electron microscopic images (SEM) and dynamic light scattering (DLS) results indicat...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.3109/02652048.2013.863395
更新日期:2014-01-01 00:00:00
abstract::Latex particles coated with either amino or carboxyl groups were labelled with 99mTc. Two labelling methods were used: (1) direct labelling with tin reduction and (2) ligand exchange with tin pyrophosphate. Labelling efficiency and the radiochemical impurities were determined by thin-layer chromatography. Effect of [S...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.3109/02652049309015313
更新日期:1993-01-01 00:00:00
abstract::Prototype fragrances, prepared from common fragrance components, were extracted with water, recovered, and characterized by gas chromatography before and after the water treatment, revealing a significant loss of the more water-soluble components. Unextracted prototype fragrances were also microencapsulated by a gelat...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.3109/02652049209021244
更新日期:1992-07-01 00:00:00
abstract::Biodegradable hollow capsules filled with fluorescently labelled bovine serum albumin (BSA) as a model drug were prepared via layer-by-layer (LbL) self-assembly of type-I collagen (COL) and hyaluronic acid (HA) using calcium carbonate micro-particles and co-precipitation method. Capsules loaded with fluorescein isothi...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.3109/02652048.2013.834995
更新日期:2014-01-01 00:00:00
abstract::A considerable effort has been spent in the past three decades to investigate various aspects of liposomes as novel drug delivery systems. In 1990, the first amphotericin B (AmB) liposomal preparation (L-AmB) under the brand name AmBisome was introduced into the market by Vestar. The successful marketing of the produc...
journal_title:Journal of microencapsulation
pub_type: 杂志文章,评审
doi:10.3109/02652049809006844
更新日期:1998-03-01 00:00:00
abstract::The aim of this study was to investigate how glass transition temperature (Tg) influenced polymer microsphere formation and degradation of three chemically, similar novel salicylatebased poly(anhydride-esters): poly[1,6-bis(o-carboxyphenoxy)hexanoate] (CPH), Tg = 59 degrees C; poly[1,8-bis(o-carboxyphenoxy)octanoate] ...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.1080/02652040600776481
更新日期:2006-09-01 00:00:00
abstract::Freeze-drying is an effective way to improve long-term physical stability of nanosuspension in drug delivery applications. Nanosuspension also known as suspension of nanoparticles. In this study, the effect of freeze-drying with different cryoprotectants on the physicochemical characteristics of resveratrol (RSV) nano...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.1080/02652048.2018.1462416
更新日期:2018-05-01 00:00:00
abstract::Hyaluronidase is a protein whose enzymatic activity is successfully employed in extravasation therapy. Taking into account that several proteins (e.g. gelatin and albumin) have been employed as natural polymers for the preparation of microspheres, this work approaches a comprehensive investigation on hyaluronidase inj...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.3109/02652049809006838
更新日期:1998-01-01 00:00:00
abstract::The body distribution of surfactant-coated and non-coated poly(methyl methacrylate) nanoparticles with a size of 131 +/- 30 nm after intravenous injection into rats was investigated. The coating materials were poloxamine 904, poloxamine 908, poloxamine 1508, poloxamer 338, and Brij 35. These materials were preselected...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.3109/02652049209021219
更新日期:1992-01-01 00:00:00
abstract::This study optimized and evaluated the conditions for surface coating of microspheres using a spray-dryer. Four formulations of Bromophenol blue (BPB)-loaded albumin microspheres were prepared using a spray-dryer, cross-linked at different concentrations and time periods. One of the optimized formulations with the des...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.1080/02652040802476187
更新日期:2008-12-01 00:00:00
abstract::Chitosan microspheres were prepared by an emulsion-phase separation technique without the use of chemical cross-linking agents; alternatively, ionotropic gelation was employed in a w/o emulsion. The possibility of three kinds of anions (tripolyphosphate, citrate and sulphate) to interact with chitosan was investigated...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.1080/02652040010000415
更新日期:2001-03-01 00:00:00
abstract::Captopril microcapsules were prepared using four different viscosity grades of ethyl cellulose (core: wall ratios 1:1, 1:2 and 1:3) by temperature induced coacervation from cyclohexane. In vitro dissolution studies in 0.1 M hydrochloric acid showed that the drug release was dependent on the core to wall ratio, the vis...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.3109/02652049009028424
更新日期:1990-01-01 00:00:00
abstract::Graft copolymer polyethylenimine-graft-poly(N-vinylpyrrolidone) (PEI-g-PVP) was prepared by coupling mono carboxyl-terminated PVP (PVP-COOH) with PEI using N,N'-dicyclohexylcarbodiimide (DCC) and N-hydroxysuccinimide (NHS) as coupling agents. In aqueous medium, PVP-g-PEI can self-assemble into stable polyion complex m...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.3109/02652048.2010.534823
更新日期:2011-01-01 00:00:00
abstract::The aim of the present study is to construct a type of polyion complex micelles made of PF127-PEI copolymer and cholic acid (CA) and to evaluate the potential of this type of micelles as a targeted drug delivery system for paclitaxel (PTX). To further improve the targeting capability of micelles, folate was also incor...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.3109/02652048.2014.940010
更新日期:2014-01-01 00:00:00
abstract::Chlorogenic acid (CA) is a natural compound used as an antioxidant in the preparation of food, drugs, and cosmetics. Due to their low stability and bioavailability, many researchers have studied the encapsulation of CA in various delivery colloidal systems. The aim of this study was to evaluate the stability of water-...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.1080/02652048.2018.1559246
更新日期:2018-09-01 00:00:00
abstract::The influence of drug particle size on the production of enteric beads by a polymer precipitation technique was investigated. Drug particle dimensions are known to play an important role in most microencapsulation techniques. Bead morphology was greatly influenced by drug particle size, and spherical shaped beads coul...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.1080/02652040050161729
更新日期:2000-11-01 00:00:00
abstract::Eudragit RS microspheres containing verapamil HCl for oral use were prepared using three different dispersing agents: aluminium tristearate, magnesium stearate and sucrose stearate, by a solvent evaporation method. The effects of the type and concentration of the dispersing agents and the inner phase polymer concentra...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.1080/02652040310001637893
更新日期:2004-03-01 00:00:00
abstract::Magnetic and pH-responsive chitosan microcapsules (MPRCMCs) were prepared by a simple sonochemical method. Superparamagnetic oleic acid modified Fe3O4 nanoparticles (OA-Fe3O4 NPs) and hydrophobic drugs could be directly loaded into MPRCMCs during sonication. The obtained microcapsules had a well-defined spherical morp...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.3109/02652048.2016.1144817
更新日期:2016-01-01 00:00:00
abstract::This article describes the physicochemical properties of chitosan-coated liposomes containing skin-protecting agents, coenzyme Q10 and alpha-lipoic acid (CCAL). CCAL had a spherical shell-core structure and liposomes inverted the surface charge from negative to positive after coating with chitosan. Compared with the u...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.3109/02652048.2014.973072
更新日期:2015-01-01 00:00:00
abstract::This work demonstrates Box-Behnken design (BBD)'s capability in exploring scientific principles governing a process, different from its use in process optimisation. We have investigated nanoprecipitation (NP) of temozolomide with polycaprolactone. Five factors, surfactant, stirring speed (SS), dropping rate (DR), phas...
journal_title:Journal of microencapsulation
pub_type: 杂志文章
doi:10.3109/02652048.2014.950710
更新日期:2015-01-01 00:00:00