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Easy genotyping of complement C3 'slow' and 'fast' allotypes by tetra-primer amplification refractory mutation system PCR.

Abstract：

:Complement C3 'slow' and 'fast' allotypes are associated with immune-mediated disorders and may affect the outcome of renal transplantation. We report a tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) that provides a rapid, reproducible and cost-effective method to genotype both complement C3 'slow' and 'fast' alleles by a single tube reaction.

journal_name

Mol Cell Probes

journal_title

Molecular and cellular probes

authors

Peruzzi B,Serra M,Pescucci C,Sica M,Lastraioli S,Rondelli T,Pedemonte S,Risitano AM,De Angioletti M,Piccioli P,Notaro R

doi

10.1016/j.mcp.2010.07.002

subject

Has Abstract

pub_date

2010-12-01 00:00:00

pages

401-2

issue

6

eissn

0890-8508

issn

1096-1194

pii

S0890-8508(10)00042-3

journal_volume

24

pub_type

杂志文章

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