当前位置： SCI文献检索 > MOLECULAR AND CELLULAR PROBES期刊下所有文献 > Multiple paternity and sperm storage in captive Hermann's tortoises, Testudo hermanni boettgeri determined from amniotic fluid adhering to the eggshell.

Multiple paternity and sperm storage in captive Hermann's tortoises, Testudo hermanni boettgeri determined from amniotic fluid adhering to the eggshell.

Abstract：

:We identified multiple paternity in 52.9% of the clutches of Hermann's tortoise Testudo hermanni boettgeri using polymorphic microsatellite markers. In addition we demonstrated sperm storage across seasons. DNA was extracted from the amniotic fluid adhering to the eggshell's inner surface, a procedure suitable for easy, non-invasive DNA sampling in conservation and breeding programs. To improve the informative value of monomorphic single tandem repeat (STR) markers we additionally analyzed single nucleotide polymorphism (SNP) variability.

journal_name

Mol Cell Probes

journal_title

Molecular and cellular probes

authors

Farke CM,Olek K,Gerding WM,Distler C

doi

10.1016/j.mcp.2015.05.009

subject

Has Abstract

pub_date

2015-08-01 00:00:00

pages

254-7

issue

4

eissn

0890-8508

issn

1096-1194

pii

S0890-8508(15)30002-5

journal_volume

29

pub_type

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