Expression patterns of miR-146a and miR-146b in mastitis infected dairy cattle.

abstract：:We have developed a new methodology for fluorescence turn-on detection of DNA methyltransferase (MTase) activity based on terminal deoxynucleotidyl transferase (TdT) using a thioflavin T probe. This method is highly selective and sensitive. The fluorescence intensity was direct proportion to Dam MTase concentration in...

journal_title：Molecular and cellular probes

authors： Ma C,Liu H,Li W,Chen H,Jin S,Wang J,Wang J

更新日期：2016-04-01 00:00:00

abstract：:Recombinase polymerase amplification assays using real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the gD gene of pseudorabies virus (PRV). Both assays were performed at 39 °C within 20 min. The sensitivity of the real-time RPA assay and the RPA L...

journal_title：Molecular and cellular probes

authors： Yang Y,Qin X,Zhang W,Li Z,Zhang S,Li Y,Zhang Z

更新日期：2017-06-01 00:00:00

abstract：:Genetic polymorphism among patients with acute lymphoblastic leukemia (ALL) is an important factor in the effectiveness and toxicity of anti-leukemic drugs. Genotyping of various polymorphisms that impact the outcome of anti-leukemic drug therapy (pharmacogenetics) presents an attractive approach for developing indivi...

journal_title：Molecular and cellular probes

authors： Kang HJ,Oh Y,Chun SM,Seo YJ,Shin HY,Kim CW,Ahn HS,Han BD

更新日期：2008-06-01 00:00:00

abstract：:Microsatellites have recently been used for linkage analysis of genetic diseases and for DNA fingerprinting in forensic medicine. In the present study the heterozygosity, PIC values and allele distributions of four microsatellites, D8S85, D8S88, D5S346 and D7S460, in an Italian population have been investigated. After...

journal_title：Molecular and cellular probes

authors： Sirchia SM,Garagiola I,De Andreis C,Gazzoli I,Gramegna M,Colucci G

更新日期：1996-04-01 00:00:00

abstract：:The cellular retinoic acid binding protein-II (CRABP-II) is an intracellular protein involved in the transmission of the vitamin A-derived signal which regulates genes responsible for lipid metabolism and adipocyte differentiation. Cellular Retinoic Acid Binding Protein-II gene (CRABP-II) (GDB 134819) is located on ch...

journal_title：Molecular and cellular probes

authors： Salazar J,Ferré R,Vallvé JC,Pocoví M,Cabezas MC,Masana L,Ribalta J

更新日期：2003-02-01 00:00:00

abstract：:This report describes detailed taxonomic and phylogenetic analysis of 15 non-tuberculous mycobacteria (NTMs) isolated from human pathological specimens in a Caribbean setting (12 slow-growers and three rapid-growers) that were not identified by cultural and biochemical tests and drug-susceptibility results. These isol...

journal_title：Molecular and cellular probes

authors： Ferdinand S,Legrand E,Goh KS,Berchel M,Mazzarelli G,Sola C,Tortoli E,Rastogi N

更新日期：2004-12-01 00:00:00

abstract：:There is a growing need for low-cost, rapid and reliable diagnostic results in veterinary medicine. Point-of-care (POC) tests have tremendous advantages over existing laboratory-based tests, due to their intrinsic low-cost and rapidity. A considerable number of POC tests are presently available, mostly in dipstick or ...

journal_title：Molecular and cellular probes

authors： Busin V,Wells B,Kersaudy-Kerhoas M,Shu W,Burgess ST

更新日期：2016-10-01 00:00:00

abstract：:The present report describes a real-time PCR-based procedure to reliably determine the quantity of Leishmania amastigotes in relation to the amount of host tissue in histological skin sections from canine and equine cases of cutaneous leishmaniasis. The novel diagnostic Leishmania-PCR has a detection limit of <0.02 am...

journal_title：Molecular and cellular probes

authors： Müller N,Hentrich B,Frey CF,Welle M

更新日期：2015-12-01 00:00:00

abstract：:The vast majority of first-trimester pregnancy losses are the consequence of numerical aberrations in fetal chromosomes, which may involve nearly all chromosomes. Although commercial probes for all chromosomes are available for multiplex ligation-dependent probe amplification (MLPA) and fluorescence in situ hybridizat...

journal_title：Molecular and cellular probes

authors： Wu T,Zhu Y,Hong L,Lin Q,Chen C,Yang J,Ye L,Huang W,Zeng Y

更新日期：2019-08-01 00:00:00

abstract：:Arcobacter is a recently described species, previously considered part of the Campylobacter family. A sensitive assay such as that provided by PCR could help to distinguish the closely related Arcobacter from Campylobacter. A PCR method to specifically detect both Campylobacter jejuni and Arcobacter butzleri in the sa...

journal_title：Molecular and cellular probes

authors： Winters DK,Slavik MF

更新日期：2000-04-01 00:00:00

abstract：:The quantitative evaluation of mosaicism for uniparental disomy (UPD) involving a restricted chromosomal region requires the availability of a sensitive and reproducible method that is capable of detecting even a small percentage of disomic cells and avoiding false positive and false negative results. The occurrence o...

journal_title：Molecular and cellular probes

authors： Russo S,Mencarelli M,Cavalleri F,Selicorni A,Cogliati F,Larizza L

更新日期：2003-12-01 00:00:00

abstract：:Members of the Mycobacterium chelonae complex (MCC), namely M. chelonae, Mycobacterium abscessus and Mycobacterium immunogenum, have been implicated in nosocomial infections and occupational respiratory illnesses like hypersensitivity pneumonitis (HP) associated with contaminated metalworking fluid (MWF) exposures. Cl...

journal_title：Molecular and cellular probes

authors： Selvaraju SB,Khan IU,Yadav JS

更新日期：2005-04-01 00:00:00

abstract：:Six stable hybridoma cell lines secreting monoclonal antibodies to human calpastatin were established. All monoclonal antibodies belong to the IgG1 subclass and recognized different epitopes on calpastatin. At least two groups were distinguished; the first group was specific for muscle-type (M-) calpastatin and the se...

journal_title：Molecular and cellular probes

authors： Yokota H,Katayama M,Hino F,Kato I,Takano E,Maki M,Hatanaka M,Murachi T

更新日期：1991-08-01 00:00:00

abstract：:To produce hybrids, one member of the parental line is genetically made male-sterile. This male-sterile trait is encoded by mitochondria so that it is maternally inherited. Consequently, the progeny of a male-sterile plant is fully sterile. Nevertheless, during the handling of cytoplasmic male-sterile seed stocks, som...

journal_title：Molecular and cellular probes

authors： Santoni S,Faivre-Rampant P,Moreau E,Bervillé A

更新日期：1991-02-01 00:00:00

abstract：:A multiplex PCR for the simultaneous detection of some pathogenic genes of enteropathogenic, enterotoxigenic and verocytotoxin-producing Escherichia coli was developed. In this study primers found in literature as well as primers to the purpose designed were used. In this way, it was possible to generate specific frag...

journal_title：Molecular and cellular probes

authors： Bottero MT,Dalmasso A,Soglia D,Rosati S,Decastelli L,Civera T

更新日期：2004-08-01 00:00:00

abstract：:The detection of hepatitis C virus (HCV) RNA by nested polymerase chain reaction (PCR) is believed to be the most reliable method to diagnose HCV infections. A pitfall of nested PCR is that it is prone to contamination. Single step reverse transcription-PCR (RT-PCR) was performed, prospectively, on 80 sera from 59 pat...

journal_title：Molecular and cellular probes

authors： Aslanzadeh J,Padilla BB,Shanley JD

更新日期：1996-06-01 00:00:00

abstract：:Using an acute promyelocytic leukemia (APL) preclinical model, we show that oncogene-specific PCR (Polymerase Chain Reaction)-based assays allow to evaluate the efficacy of immunotherapy combining all-trans retinoic acid (ATRA) and a DNA-based vaccine targeting the promyelocytic leukemia-retinoic acid receptor alpha (...

journal_title：Molecular and cellular probes

authors： Pokorna K,Le Pogam C,Chopin M,Balitrand N,Reboul M,Cassinat B,Chomienne C,Padua RA,Pla M

更新日期：2013-02-01 00:00:00

abstract：:Detection of Ehrlichia chaffeensis is necessary to study interactions between the parasite and its vertebrate and invertebrate hosts. The purpose of this study was to develop a sensitive, specific PCR assay for E. chaffeensis based on the outer membrane protein gene, p28. Candidate primer sets were identified and rank...

journal_title：Molecular and cellular probes

authors： Wagner ER,Bremer WG,Rikihisa Y,Ewing SA,Needham GR,Unver A,Wang X,Stich RW

更新日期：2004-04-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) is responsible for severe infections in immunocompromised patients. Viral load has recently been identified as one of the major risk factors for subsequent development of HCMV disease. In this context, we developed a protocol allowing rapid, sensitive and precise quantification of HCMV DNA...

journal_title：Molecular and cellular probes

authors： Poirier-Toulemonde AS,Imbert-Marcille BM,Ferré-Aubineau V,Besse B,Le Roux MG,Cantarovich D,Billaudel S

更新日期：1997-02-01 00:00:00

abstract：:Advanced reflectance-based optical techniques for in vivo imaging often suffer from low contrast between neoplastic and normal tissue and are unable to image early biomolecular changes associated with carcinogenesis, thus limiting their clinical value. In this study, we exploit the resonance light scattering property ...

journal_title：Molecular and cellular probes

authors： Kah JC,Olivo MC,Lee CG,Sheppard CJ

更新日期：2008-02-01 00:00:00

abstract：:Both Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV) can cause high mortality and morbidity in Muscovy ducklings. MDPVs and GPVs share high nucleotide identity, which can cause errors during differential diagnosis. In this study, the NS genes of both MDPVs and GPVs were chosen for the design of specific prim...

journal_title：Molecular and cellular probes

authors： Wan C,Cheng L,Chen C,Liu R,Shi S,Fu G,Chen H,Fu Q,Huang Y

更新日期：2019-10-01 00:00:00

abstract：:An assay for rapid detection of herpes simplex virus in infected cells is described. The assay utilizes in situ hybridization with photobiotin-labelled double-stranded DNA probes prepared from HSV-1 DNA cloned in plasmid vectors. The assay provided an alternative method for earlier detection of virus in cell cultures ...

journal_title：Molecular and cellular probes

authors： Chantratita W,Henchal EA,Yoosook C

更新日期：1989-12-01 00:00:00

abstract：:Some receptor tyrosine kinase genes are mutated in inherited and somatically acquired human cancers. To permit mutational analysis, the complete genomic structure of the human EPHA1 gene on chromosome 7q34 was determined and oligonucleotide pairs were designed to amplify coding regions. The gene contains 18 exons, two...

journal_title：Molecular and cellular probes

authors： Owshalimpur D,Kelley MJ

更新日期：1999-06-01 00:00:00

abstract：:Actinobacillus pleuropneumoniae is the etiological agent of swine contagious pleuropneumoniae, which is distributed globally and associated with severe economic losses in the pig rearing industry. In this study, a real-time recombinase polymerase amplification assay (real-time RPA) based on the apxIVA gene was develop...

journal_title：Molecular and cellular probes

authors： Li R,Wang J,Liu L,Zhang R,Hao X,Han Q,Wang J,Yuan W

更新日期：2019-06-01 00:00:00

abstract：AIM:Though Endostar (ES) could inhibit tumor growth by inhibiting tumor angiogenesis, other possible mechanisms have been less reported. This study aims to investigate the role of ES in the treatment of lung cancer from the perspective of macrophage-mediated epithelial mesenchymal transformation (EMT). METHODS:THP1 ce...

journal_title：Molecular and cellular probes

authors： Shen Y,Chen Q,Li L

更新日期：2019-06-01 00:00:00

abstract：:Variable number tandem repeat (VNTR) is a frequently employed typing method of Mycobacterium avium paratuberculosis (MAP) isolates. Based on whole genome sequencing in a previous study, allelic diversity at some VNTR loci seems to over- or under-estimate the actual phylogenetic variance among isolates. Interestingly, ...

journal_title：Molecular and cellular probes

authors： Fawzy A,Zschöck M,Ewers C,Eisenberg T

更新日期：2016-06-01 00:00:00

abstract：:DNA aptamers (PSA-H and MT32) were applied for the detection of Apple stem pitting virus (ASPV) isolates using an Enzyme-Linked Oligonucleotide Assay (ELONA) and Western blot analysis. The specificity and effectiveness of aptamers were verified in comparison to a conventional Enzyme Linked Immunosorbent Assay (ELISA)....

journal_title：Molecular and cellular probes

authors： Komorowska B,Hasiów-Jaroszewska B,Minicka J

更新日期：2017-12-01 00:00:00

abstract：:Angiostrongylus cantonensis, a rat lungworm, can cause eosinophilic meningitis and angiostrongyliasis in humans following ingestion of contaminated foods or intermediate/paratenic hosts with infective larvae. The snail Achatina fulica is one of the important intermediate hosts of A. cantonensis and is commonly eaten b...

journal_title：Molecular and cellular probes

authors： Liu CY,Song HQ,Zhang RL,Chen MX,Xu MJ,Ai L,Chen XG,Zhan XM,Liang SH,Yuan ZG,Lin RQ,Zhu XQ

更新日期：2011-08-01 00:00:00

abstract：:In this paper we describe a reverse-transcription nested polymerase chain reaction method for detecting human astrovirus serotype 1. It has been evaluated on 56 UK diarrhoeal stool specimens and six non-UK specimens. The method has greater sensitivity than electron microscopy and may be a useful test in areas such as ...

journal_title：Molecular and cellular probes

authors： Shi M,Sikotra S,Lee T,Kurtz JB,Getty B,Hart CA,Myint SH

更新日期：1994-12-01 00:00:00

abstract：:Quantification of nucleic acids, especially of mRNA, is increasingly important in biomedical research. The recently developed quantitative real-time polymerase chain reaction (PCR) - a highly sensitive technology for the rapid, accurate and reproducible quantification of gene expression - offers major advantages over ...

journal_title：Molecular and cellular probes

authors： Fronhoffs S,Totzke G,Stier S,Wernert N,Rothe M,Brüning T,Koch B,Sachinidis A,Vetter H,Ko Y

更新日期：2002-04-01 00:00:00