Evaluation of two aneuploidy screening tests for chorionic villus samples: Multiplex ligation-dependent probe amplification and fluorescence in situ hybridization.

abstract：:The single nucleotide polymorphism (SNP) genotyping is currently considered as a particularly valuable tool for the diagnosis of different pathologies. For this reason, over the past several years a great deal of effort has been devoted to developing accurate, rapid, and cost-effective technologies for SNP analysis. A...

journal_title：Molecular and cellular probes

authors： Etlik O,Koksal V,Arican-Baris ST,Baris I

更新日期：2011-08-01 00:00:00

abstract：:The INK4a/ARF locus encodes two cell cycle-regulatory proteins, p16(INK4a)and p14(ARF). Inactivation of the p16(INK4a)(MTS1) tumor suppressor gene by mutations, promoter methylation or gene deletions is a common event in the development of many different human tumors. The present report describes a novel polyA mononuc...

journal_title：Molecular and cellular probes

authors： Chaubert P,Burri N,Cousin P,Shaw P

更新日期：2001-06-01 00:00:00

abstract：:Many highly homologous genes are present in the murine major histocompatibility complex (MHC) class I gene family. Consequently, it is difficult to distinguish between RNA transcripts of individual class I genes solely on the basis of nucleic acid hybridization analysis using DNA probes over 50 base pairs long. To avo...

journal_title：Molecular and cellular probes

authors： Mellor AL

更新日期：1987-09-01 00:00:00

abstract：:In a previous study, we reported the performance of a PCR assay amplifying 287-bp of the 16S rRNA gene of thermo-tolerant Campylobacter (C. jejuni, C. lari, C. coli) through an international ring-trial involving 12 participating laboratories. Based on the validated set of primers, a LightCycler real-time PCR assay (LC...

journal_title：Molecular and cellular probes

authors： Perelle S,Josefsen M,Hoorfar J,Dilasser F,Grout J,Fach P

更新日期：2004-10-01 00:00:00

abstract：:Recombinase polymerase amplification assays using real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the gD gene of pseudorabies virus (PRV). Both assays were performed at 39 °C within 20 min. The sensitivity of the real-time RPA assay and the RPA L...

journal_title：Molecular and cellular probes

authors： Yang Y,Qin X,Zhang W,Li Z,Zhang S,Li Y,Zhang Z

更新日期：2017-06-01 00:00:00

abstract：:DNA samples of 2303 individuals from nine different population groups were screened for variant -175g-->t in the promoter region of the low-density lipoprotein receptor (LDLR) gene. The -175g-->t variant detected at carrier frequencies of 3-10% in different African population groups was absent in the Caucasian and Asi...

journal_title：Molecular and cellular probes

authors： Hoogendijk CF,Scholtz CL,Pimstone SM,Ehrenborg E,Kastelein JJ,Defesche JC,Thiart R,du Plessis L,de Villiers JN,Zaahl MG,Delport R,Rubinsztein DC,Raffel LJ,Grim CE,Mediene-Benchekor S,Amouyel P,Brousseau T,Steyn K,Lomb

更新日期：2003-08-01 00:00:00

abstract：:In this study, we identified, using an established oligonucleotide microarray platform for the parasitic nematode Haemonchus contortus, transcripts that are 'conserved' between serum-activated and non-activated L3s of Ancylostoma caninum (aL3 and L3, respectively) and H. contortus by cross-species hybridization (CSH) ...

journal_title：Molecular and cellular probes

authors： Cantacessi C,Loukas A,Campbell BE,Mulvenna J,Ong EK,Zhong W,Sternberg PW,Otranto D,Gasser RB

更新日期：2009-02-01 00:00:00

abstract：:Angiostrongylus cantonensis, a rat lungworm, can cause eosinophilic meningitis and angiostrongyliasis in humans following ingestion of contaminated foods or intermediate/paratenic hosts with infective larvae. The snail Achatina fulica is one of the important intermediate hosts of A. cantonensis and is commonly eaten b...

journal_title：Molecular and cellular probes

authors： Liu CY,Song HQ,Zhang RL,Chen MX,Xu MJ,Ai L,Chen XG,Zhan XM,Liang SH,Yuan ZG,Lin RQ,Zhu XQ

更新日期：2011-08-01 00:00:00

abstract：:Complement C3 'slow' and 'fast' allotypes are associated with immune-mediated disorders and may affect the outcome of renal transplantation. We report a tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) that provides a rapid, reproducible and cost-effective method to genotype both complement C3 's...

journal_title：Molecular and cellular probes

authors： Peruzzi B,Serra M,Pescucci C,Sica M,Lastraioli S,Rondelli T,Pedemonte S,Risitano AM,De Angioletti M,Piccioli P,Notaro R

更新日期：2010-12-01 00:00:00

abstract：:Yersinia pestis, the causative agent of plague mainly infects rodents, while humans are the accidental host. The conventional diagnostic methods available for Y. pestis exhibit cross-reactivity with other enteropathogenic bacteria which makes its detection difficult. Rapid and reliable point-of-care detection of Y. pe...

journal_title：Molecular and cellular probes

authors： Singh R,Pal V,Tripathi NK,Goel AK

更新日期：2020-12-01 00:00:00

abstract：:We have developed a single tube TaqMan(®) real-time PCR assay that differentiates the full-length and truncated erm(41) gene to predict inducible resistance to clarithromycin in Mycobacterium abscessus. A study of 87 clinical isolates found this assay to be 90.8% concordant to conventional drug susceptibility testing ...

journal_title：Molecular and cellular probes

authors： Zhu YC,Mitchell KK,Nazarian EJ,Escuyer VE,Musser KA

更新日期：2015-12-01 00:00:00

abstract：:Actinobacillus pleuropneumoniae is the etiological agent of swine contagious pleuropneumoniae, which is distributed globally and associated with severe economic losses in the pig rearing industry. In this study, a real-time recombinase polymerase amplification assay (real-time RPA) based on the apxIVA gene was develop...

journal_title：Molecular and cellular probes

authors： Li R,Wang J,Liu L,Zhang R,Hao X,Han Q,Wang J,Yuan W

更新日期：2019-06-01 00:00:00

abstract：:Human infection with the parasitic nematode Enterobius vermicularis occurs worldwide, particularly in children. Although its prevalence may exceed 35% in some parts of the world, molecular studies of E. vermicularis in humans are limited. The aim of the present study was to investigate the genetic variation within E. ...

journal_title：Molecular and cellular probes

authors： Piperaki ET,Spanakos G,Patsantara G,Vassalou E,Vakalis N,Tsakris A

更新日期：2011-04-01 00:00:00

abstract：:The phenotypic detection of Campylobacter concisus, a species of considerable genomic and phenotypic heterogeneity, has proven to be rather tedious in the past. Although alternative methods like DNA:DNA hybridization, immunotyping or whole-cell protein electrophoresis are valuable for the specific detection of C. conc...

journal_title：Molecular and cellular probes

authors： Bastyns K,Chapelle S,Vandamme P,Goossens H,De Wachter R

更新日期：1995-08-01 00:00:00

abstract：:A polymerase chain reaction (PCR) method specific for Mycoplasma gallisepticum (MG) was evaluated. The PCR method was found to detect as few as two colour changing units (CCU) of MG and did not give false positive reactions with other avian mycoplasmas. In chickens inoculated with either MG or Mycoplasma synoviae (MS)...

journal_title：Molecular and cellular probes

authors： Slavik MF,Wang RF,Cao WW

更新日期：1993-12-01 00:00:00

abstract：:Hypertriglyceridemia (HTG) plays an important role in the development and progression of atherosclerosis. It is inherited in an autosomal dominant pattern with a frequency of approximately 1:1,000,000 worldwide. Previous study has demonstrated that more than six genes underlie this disorder. In addition, copy number v...

journal_title：Molecular and cellular probes

authors： Li JJ,Chen YQ,Fan LL,Jin JY,Guo S,Xiang R

更新日期：2018-02-01 00:00:00

abstract：:Genetic polymorphism among patients with acute lymphoblastic leukemia (ALL) is an important factor in the effectiveness and toxicity of anti-leukemic drugs. Genotyping of various polymorphisms that impact the outcome of anti-leukemic drug therapy (pharmacogenetics) presents an attractive approach for developing indivi...

journal_title：Molecular and cellular probes

authors： Kang HJ,Oh Y,Chun SM,Seo YJ,Shin HY,Kim CW,Ahn HS,Han BD

更新日期：2008-06-01 00:00:00

abstract：:Citrus plants are natural hosts of five viroid species and a large number of sequence variants. Because of their small size, viroids lend themselves to various RT-PCR approaches for their detection and further characterization. The one-step RT-PCR approach proposed here is based on the synthesis of viroid-cDNA by reve...

journal_title：Molecular and cellular probes

authors： Bernad L,Duran-Vila N

更新日期：2006-04-01 00:00:00

abstract：:Several studies have shown that BK viral load in plasma and urine are reliable markers for the detection of BK virus associated nephropathy (BKVAN) in renal transplant patients. We developed a quantitative real time PCR assay based on TaqMan technology for the measurement of BK viral load in plasma and urine. Consider...

journal_title：Molecular and cellular probes

authors： Mitui M,Leos NK,Lacey D,Doern C,Rogers BB,Park JY

更新日期：2013-10-01 00:00:00

abstract：:Beet Necrotic Yellow Vein Virus (BNYVV) was detected by enzyme-linked immunosorbent assay (ELISA) and RNA/DNA dot hybridization using either radiolabelled or non-radioactive probes. Dot hybridization specifically distinguished isolates that could not be distinguished by ELISA. The detection thresholds for ELISA, hybri...

journal_title：Molecular and cellular probes

authors： Sakamoto H,Lemaire O,Merdinoglu D,Guesdon JL

更新日期：1989-06-01 00:00:00

abstract：:As part of the development of the ligase chain reaction (LCR) into a tool which can be used by a wide variety of researchers, we have investigated several analytical detection systems for the products of this amplification reaction. While early work with this technology has used gel electrophoresis to separate the LCR...

journal_title：Molecular and cellular probes

authors： Winn-Deen ES,Batt CA,Wiedmann M

更新日期：1993-06-01 00:00:00

abstract：:The aim of the present study was to evaluate a mixed phase DNA hybridization assay for detection of Bordetella pertussis and Bordetella parapertussis in nasopharyngeal aspirates from patients with suspected pertussis. Among 179 consecutive patients with own or parental suspicion of pertussis, the diagnosis was confirm...

journal_title：Molecular and cellular probes

authors： Reizenstein E,Morfeldt E,Granström G,Granström M,Löfdahl S

更新日期：1990-08-01 00:00:00

abstract：:Genomic deletions/duplications detected by array comparative genomic hybridization (aCGH) should be confirmed by an independent technology. This approach allows also to test, at low cost, inheritance of the imbalance. In the present study we explored the use of quantitative PCR (qPCR) to confirm aCGH-detected potentia...

journal_title：Molecular and cellular probes

authors： Baldan F,Passon N,Burra S,Demori E,Russo PD,Damante G

更新日期：2019-08-01 00:00:00

abstract：:The human SNAIL is an important developmental protein involved in the formation of mesoderm and neural crest. The protein contains three classic and one atypical zinc-finger motif. The SNAI1 gene is composed of three exons. We have identified three SNPs in non-coding regions, two in the 5'UTR and one in intron 1, whic...

journal_title：Molecular and cellular probes

authors： Okajima K,Paznekas WA,Burstyn T,Jabs EW

更新日期：2001-02-01 00:00:00

abstract：:The flagellin genes of four Burkholderia pseudomallei and two Burkholderia cepacia clinical isolates were studied by a polymerase chain reaction (PCR)-based isolation method using the same pair of primers. The PCR-amplification products of the isolates showed a single band of about 1.1 kb, which is similar to a type I...

journal_title：Molecular and cellular probes

authors： Tungpradabkul S,Wajanarogana S,Tunpiboonsak S,Panyim S

更新日期：1999-04-01 00:00:00

abstract：:This study evaluates five cryptic plasmid-derived DNA probes in a 4-h slot-blot hybridization assay for the detection of Chlamydia trachomatis in cultures and clinical specimens. The probes, consisting of either the entire cloned 7.5 kbp cryptic plasmid pSE8 or one of four Hin dIII/Eco RI fragments measuring 710, 1055...

journal_title：Molecular and cellular probes

authors： Cano RJ,Murrieta CM,Spaulding DC,Pascual A

更新日期：1991-12-01 00:00:00

abstract：:A PCR-enzyme-linked immunosorbent assay (PCR-ELISA) was developed for direct identification of Pseudomonas aeruginosa from positive BACTEC blood culture bottles. PCR primers were designed to target a 249 bp sequence of the oprI gene in P. aeruginosa. Biotin-labeled probe (PA3) targeted to the species-specific motif we...

journal_title：Molecular and cellular probes

authors： Kurupati P,Kumarasinghe G,Laa Poh C

更新日期：2005-12-01 00:00:00

abstract：:The spectrum of cystic fibrosis (CF) mutations has been determined in many populations of different ethnic and geographic origins. However, in the south of Europe, the commonest mutation, delta F508, accounts for only about 50% of CF chromosomes, while identification of most of the other mutant alleles has not been ac...

journal_title：Molecular and cellular probes

authors： Férec C,Novelli G,Verlingue C,Quéré I,Dallapiccola B,Audrézet MP,Mercier B

更新日期：1995-04-01 00:00:00

abstract：:A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the nucleocapsid phosphoprotein gene of infectious bronchitis virus (IBV) was developed. The detection limits for the IBV RT-LAMP assay were 10(1) 50% egg infection dose (EID(50)) per 50 microl of titrated viruses and no cross-rea...

journal_title：Molecular and cellular probes

authors： Chen HT,Zhang J,Ma YP,Ma LN,Ding YZ,Liu XT,Cai XP,Ma LQ,Zhang YG,Liu YS

更新日期：2010-04-01 00:00:00

abstract：:Detection of Ehrlichia chaffeensis is necessary to study interactions between the parasite and its vertebrate and invertebrate hosts. The purpose of this study was to develop a sensitive, specific PCR assay for E. chaffeensis based on the outer membrane protein gene, p28. Candidate primer sets were identified and rank...

journal_title：Molecular and cellular probes

authors： Wagner ER,Bremer WG,Rikihisa Y,Ewing SA,Needham GR,Unver A,Wang X,Stich RW

更新日期：2004-04-01 00:00:00